LOCUS       RR201250                8322 bp    DNA     circular  SYN 
DEFINITION  Nos2 (Myc-DDK-tagged ORF) - Rat nitric oxide synthase 2, inducible (Nos2), (10 ug)
ACCESSION   NM_012611
VERSION     NM_012611.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4479..4570 
                     /label="Myc-DDK"  
     misc_feature    4600..5185 
                     /label="PolyA signal"  
     misc_feature    4657..4676 
                     /label="Restriction Enzyme"  
     misc_feature    5461..6133 
                     /label="ColE1"  
     misc_feature    6563..7357 
                     /label="Kan/Neo"  
     misc_feature    7392..7694 
                     /label="SV40 ori"  
     misc_feature    7780..8257 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4461..4467 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4461 
                     /label="Nos2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGCCCC TGGAAGTTTC TCTTCAGAGT CAAATCCTAC CAAGGTGACC 
     1081 TGAAAGAGGA AAAGGACATT AACAACAACG TGGAGAAAAC CCCAGGTGCT ATTCCCAGCC 
     1141 CAACAACACA GGATGACCCT AAGAGTCACA AGCATCAAAA TGGTTTCCCC CAGTTCCTCA 
     1201 CTGGGACTGC ACAGAATGTT CCAGAATCCC TGGACAAGCT GCATGTGACT CCATCGACCC 
     1261 GCCCACAGCA CGTGAGGATC AAAAACTGGG GCAATGGAGA GATTTTTCAC GACACCCTTC 
     1321 ACCACAAGGC CACCTCGGAT ATCTCTTGCA AGTCCAAATT ATGCATGGGG TCCATCATGA 
     1381 ACTCCAAGAG TTTGACCAGA GGACCCAGAG ACAAGCCCAC CCCAGTGGAG GAGCTTCTGC 
     1441 CTCAAGCCAT TGAATTCATT AACCAGTATT ATGGCTCCTT CAAAGAGGCA AAAATAGAGG 
     1501 AACATCTGGC CAGGCTGGAA GCCGTAACAA AGGAAATAGA AACAACAGGA ACCTACCAGC 
     1561 TCACTCTGGA TGAGCTCATC TTTGCCACCA AGATGGCCTG GAGGAACGCC CCTCGCTGCA 
     1621 TCGGCAGGAT TCAGTGGTCC AACCTGCAGG TCTTCGATGC CCGGAGCTGT AGCACTGCAT 
     1681 CAGAAATGTT CCAGCATATC TGCAGACACA TACTTTACGC CACTAACAGT GGCAACATCA 
     1741 GGTCGGCCAT TACTGTGTTC CCCCAGCGGA GCGATGGGAA GCATGACTTC CGGATCTGGA 
     1801 ATTCCCAGCT CATCCGGTAC GCTGGCTACC AGATGCCCGA TGGCACCATC AGAGGGGATC 
     1861 CTGCCACCTT GGAGTTCACC CAGTTGTGCA TCGACCTGGG CTGGAAGCCC CGCTATGGCC 
     1921 GCTTCGATGT GCTGCCTCTG GTCCTGCAGG CTCACGGTCA AGATCCAGAG GTCTTTGAAA 
     1981 TCCCTCCTGA TCTTGTGCTG GAGGTGACCA TGGAGCATCC CAAGTACGAG TGGTTCCAGG 
     2041 AGCTCGGGCT GAAGTGGTAT GCGCTGCCTG CCGTGGCCAA CATGCTCCTG GAGGTGGGTG 
     2101 GCCTCGAGTT CCCAGCCTGC CCCTTCAATG GTTGGTACAT GGGCACCGAG ATTGGAGTCC 
     2161 GAGACTTCTG TGACACACAG CGCTACAACA TCCTGGAGGA AGTGGGCAGG AGCATGGGCC 
     2221 TGGAGACCCA CACACTGGCC TCCCTCTGGA AAGACCGGGC TGTCACCGAG ATCAATGCAG 
     2281 CTGTGCTCCA TAGTTTTCAG AAGCAGAATG TGACCATCAT GGACCACCAC ACAGCCTCAG 
     2341 AGTCCTTCAT GAAGCACATG CAGAATGAGT ACCGGGCCCG AGGAGGCTGC CCTGCAGACT 
     2401 GGATTTGGCT GGTCCCTCCG GTGTCCGGGA GCATCACCCC TGTGTTCCAC CAGGAGATGT 
     2461 TGAACTACGT CCTATCTCCA TTCTACTACT ACCAGATCGA GCCCTGGAAG ACCCACATCT 
     2521 GGCAGGATGA GAAGCTGAGG CCCAGGAGGA GAGAGATCCG GTTCACAGTC TTGGTGAAAG 
     2581 CGGTGTTCTT TGCTTCTGTG CTAATGCGGA AGGTCATGGC TTCCCGCGTC AGAGCCACAG 
     2641 TCCTCTTTGC TACTGAGACA GGAAAGTCGG AAGCGCTAGC CAGGGACCTG GCTGCCTTGT 
     2701 TCAGCTACGC CTTCAACACC AAGGTTGTCT GCATGGAACA GTATAAGGCA AACACCTTGG 
     2761 AAGAGGAACA ACTACTGCTG GTGGTTACAA GCACATTTGG CAATGGAGAC TGCCCCAGCA 
     2821 ATGGGCAGAC TCTGAAGAAA TCTCTGTTCA TGATGAAAGA ACTCGGGCAT ACCTTCAGGT 
     2881 ATGCGGTATT TGGCCTGGGC TCCAGCATGT ACCCTCAGTT CTGTGCCTTT GCTCATGACA 
     2941 TCGACCAGAA ACTGTCTCAC CTGGGAGCCT CCCAGCTTGC CCCAACCGGA GAAGGGGACG 
     3001 AACTCAGCGG GCAGGAGGAC GCCTTCCGCA GCTGGGCTGT GCAAACCTTC CGGGCAGCCT 
     3061 GTGAGACGTT CGATGTTCGA AGCAAACATT GCATTCAGAT CCCGAAACGC TACACTTCCA 
     3121 ACGCAACATG GGAGCCAGAG CAGTACAAGC TCACCCAGAG CCCAGAGTCT CTAGACCTCA 
     3181 ACAAAGCTCT CAGCAGCATC CACGCCAAGA ACGTGTTCAC CATGAGGCTG AAATCCCTCC 
     3241 AGAATCTGCA GAGTGAGAAG TCCAGCCGCA CCACCCTCCT TGTTCAACTC ACCTTCGAGG 
     3301 GCAGCCGAGG CCCCAGCTAC CTACCTGGGG AACACCTGGG GATTTTCCCA GGCAACCAGA 
     3361 CGGCCCTGGT GCAAGGGATC TTGGAGCGAG TTGTGGATTG TTCTTCGCCA GACCAAACTG 
     3421 TGTGCCTGGA GGTTCTAGAT GAGAGTGGCA GCTACTGGGT CAAAGACAAG AGGCTTCCCC 
     3481 CCTGCTCACT CAGGCAAGCC CTCACCTACT TCCTGGACAT CACTACCCCT CCCACCCAGC 
     3541 TGCAGCTCCA CAAGCTGGCC CGCTTTGCCA CGGAAGAGAC GCACAGGCAG AGGTTGGAGG 
     3601 CCTTGTGTCA GCCCTCAGAG TACAACGATT GGAAGTTCAG CAACAACCCC ACGTTCCTGG 
     3661 AGGTGCTGGA AGAGTTCCCA TCATTGCGTG TGCCTGCTGC CTTCCTGCTG TTGCAGCTCC 
     3721 CCATTCTGAA GCCCCGCTAC TACTCCATCA GCTCCTCCCA GGACCACACC CCCTCGGAGG 
     3781 TCCACCTCAC TGTGGCTGTG GTCACCTATC GCACCCGAGA TGGTCAGGGT CCCCTGCACC 
     3841 ATGGCGTCTG CAGCACTTGG ATCAATAACC TGAAGCCCGA AGACCCAGTG CCCTGCTTTG 
     3901 TGCGGAGTGT CAGTGGCTTC CAGCTCCCTG AGGACCCCTC CCAGCCCTGC ATCCTCATTG 
     3961 GGCCCGGTAC AGGCATTGCC CCCTTCCGAA GTTTCTGGCA GCAGCGGCTC CATGACTCTC 
     4021 AGCACAGAGG GCTCAAAGGA GGCCGCATGA CCTTGGTGTT TGGGTGCAGG CACCCAGAGG 
     4081 AGGACCACCT CTATCAGGAA GAAATGCAGG AGATGGTCCG CAAGGGAGTG TTGTTCCAGG 
     4141 TGCACACAGG CTACTCCCGG CTGCCCGGAA AACCCAAGGT CTACGTTCAA GACATCCTGC 
     4201 AGAAAGAGCT GGCCGACGAG GTGTTCAGCG TGCTCCACGG GGAGCAGGGC CACCTTTATG 
     4261 TTTGTGGCGA TGTGCGCATG GCTCGGGATG TGGCTACCAC TTTGAAGAAG CTGGTGGCCG 
     4321 CCAAGCTGAA CTTGAGTGAG GAGCAGGTTG AGGATTACTT CTTCCAGCTC AAGAGCCAGA 
     4381 AACGTTATCA TGAGGATATC TTCGGTGCGG TCTTTTCCTA TGGAGCAAAA AAGGGCAACA 
     4441 CCTTGGAGGA GCCCAAAGGC ACAAGACTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4501 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4561 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4621 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4681 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4741 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4801 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4861 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4921 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4981 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5041 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5101 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5161 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5221 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5281 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5341 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5401 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5461 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5521 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5581 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5641 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5701 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5761 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5821 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5881 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5941 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6001 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6061 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6121 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6181 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6241 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6301 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6361 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6421 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6481 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6541 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6601 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6661 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6721 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6781 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6841 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6901 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6961 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7021 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7081 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7141 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7201 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7261 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7321 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7381 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7441 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7501 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7561 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7741 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7861 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7921 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7981 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8041 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8101 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8161 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8221 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8281 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//