LOCUS       RR201131                8280 bp    DNA     circular  SYN 
DEFINITION  Egf (Myc-DDK-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug)
ACCESSION   NM_012842
VERSION     NM_012842.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4437..4528 
                     /label="Myc-DDK"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    4615..4634 
                     /label="Restriction Enzyme"  
     misc_feature    5419..6091 
                     /label="ColE1"  
     misc_feature    6521..7315 
                     /label="Kan/Neo"  
     misc_feature    7350..7652 
                     /label="SV40 ori"  
     misc_feature    7738..8215 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4425 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="Egf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTTCTCG CTCACCTTCC TGTCGGTGTT TTTAAAGATT ACTGTACTCA 
     1081 GTGTCACAGC ACAGCAGACC AGGAACTGTC AGTCAGGTCC TCTCGAGAGA AGCGGGACTA 
     1141 CCACGTATGC CGCCGCCGGT CCTCCCAGGT TCCTGATTTT CTTACAAGGA AACAGCATCT 
     1201 TTCGGATTAA CACAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCC GGCGTCTCAG 
     1261 TGGTCATGGA TTTTCATTAC AAGGAAGAGA GACTCTATTG GGTGGATTTA GAAAGACAAC 
     1321 TTTTGCAAAG AGTTTTCTTT AATGGGTCAG GACAAGAGAC AGTGTGCAAG GTGGATAAGA 
     1381 ATGTGTCTGG GCTGGCCATA AACTGGATAG ATGGGGAGAT TCTCCGGACG GACCGATGGA 
     1441 AGGGAGTCAT CACAGTAACA GATATGAACG GGAACAATTC CCGTGTTCTT CTGAGTTCCT 
     1501 TAAAACGTCC TGCAAATATA TTAGTGGATC CAACAGAGAG GTTGATATTT TGGTCTTCAG 
     1561 TGGTGACTGG CAACCTTCAC AGAGCAGATC TCGGGGGTAT GGATGTAAAA ACACTGCTGG 
     1621 AGGCACCAGA GAGGATATCA GTGCTGATTC TGGATATCCT GGACAAAAGG CTCTTCTGGG 
     1681 CTCAGGACGG TAGAGAAGGA AGCCACGGTT ACATTCACTC CTGTGACTAT AACGGTGGCT 
     1741 CCATCCATCA TATCAGACAT CAAGCACGGC ACGATTTGCT TACTATGGCC ATTTTCGGTG 
     1801 ACAAGATCTT ATACTCAGCA CTGAAAGAGA AGGCGATTTG GATAGCCGAC AAACACACTG 
     1861 GGAAGAATGT GGTTCGAGTT AACCTCGATC CAGCCTCTGT GCCGCCAAGA GAACTGAGAG 
     1921 TCGTGCACCT ACATGCACAG CCCGGGACAG AGAACCGTGC TCAGGCCTCT GACTCCGAAC 
     1981 GATGCAAACA GAGAAGAGGA CAGTGTCTCT ACAGTCTCTC TGAGCGAGAC CCCAACTCAG 
     2041 ACTCGTCGGC ATGCGCTGAA GGCTATACGT TAAGCCGAGA CCGGAAGTAC TGCGAAGATG 
     2101 TCAATGAGTG TGCCTTGCAG AATCACGGCT GTACTCTTGG GTGTGAAAAC ATCCCTGGAT 
     2161 CCTATTACTG CACATGCCCT ACAGGCTTTG TTCTGCTTCC TGATGGGAAA CGATGTCACG 
     2221 AACTTGTTGC CTGTCCAGGC AACAGATCAG AGTGTAGCCA TGATTGCATC CTGACATCAG 
     2281 ATGGTCCTCT GTGCATCTGT CCAGCAGGTT CAGTGCTCGG AAAAGATGGG AAGACATGCA 
     2341 CTGGTTGTTC CTTCTCCGAT AATGGTGGAT GCAGCCAGAT CTGCCTTCCT CTCAGCCTAG 
     2401 CATCCTGGGA ATGTGATTGC TTTCCTGGGT ACGACCTACA ATTGGACCGA AAGAGCTGTG 
     2461 CAGCTTCCAT GGGACCGCAG CCATTTTTAC TGTTTGCAAA TTCCCAGGAC ATACGACACA 
     2521 TGCATTTTGA TGGAACAGAC TACAAAACTC TGCTCAGCCG GCAGATGGGA ATGGTTTTTG 
     2581 CCTTGGATTA TGACCCCGTG GAAAGCAAGA TATATTTTGC ACAGACAGCC CTGAAGTGGA 
     2641 TAGAGAGGGC TAATCTGGAT GGCTCCCAGC GAGAAAGACG GATCACGGAA GGAGTAGACA 
     2701 CGCCAGAAGG TCTTGCCGTG GACTGGATTG GCCGGAGAAT CTACTGGACG GACAGTGGGA 
     2761 AGTCTGTCAT TGAAGGGAGT GATTTGAGCG GGAAGCATCA TCAAATAATC ATCAAAGAGA 
     2821 GCATCTCAAG GCCACGAGGA ATAGCTGTGC ATCCAAAGGC CAGGAGACTA TTCTGGACGG 
     2881 ACACGGGGAT GTCTCCGCGG ATTGAAAGCT CTTCCCTTCA AGGTTCTGAC CGGACGCTGA 
     2941 TAGCCAGCTC TAATCTACTG GAACCCAGTG GAATCGCGAT TGACTACTTA ACAGACACTT 
     3001 TGTACTGGTG TGACACCAAG CTGTCTGTGA TTGAAATGGC CGATCTAGAT GGTTCCAAAC 
     3061 GCCGCAGACT TACCCAGAAC GATGTAGGTC ACCCATTCTC TCTAGCTGTG TTTGAGGATC 
     3121 ACGTGTGGTT CTCGGATTGG GCTATCCCAT CGGTAATAAG GGTGAACAAG AGGACTGGTC 
     3181 AAAACAGGGT ACGTCTCCGA GGCAGCATGC TGAAGCCCTC GTCACTGGTT GTGGTCCACC 
     3241 CATTGGCAAA ACCAGGTGCA GACCCCTGCT TACACAGGAA TGGAGGCTGT GAACACATCT 
     3301 GCCAAGAGAG CCTGGGCACG GCTCAGTGTC TGTGTCGGGA AGGATTCGTG AAGGCCCCAG 
     3361 ATGGGAAAAT GTGTCTCACT CGGAAGGATG ATCAGATACT GGCCGGTGAC AATGCTGATC 
     3421 TTAGTAAAGA GGTGGCATCG TTGGACAACT CCCCTAAGGC TTATGTACCA GACGATGATA 
     3481 GGACAGAGTC CTCCACACTA GTGGCTGAGA TCATGGTGTC AGGGCTGAAC TATGAAGATG 
     3541 ACTGCGGCCC TGGTGGGTGT GGCAGCCATG CCCACTGTAT TTCAGAGGGA GAGGCAGCTG 
     3601 TGTGTCAGTG TTTGAAAGGA TTTGCTGGCG ATGGAAACCT GTGTTCTGAT ATAGACGAAT 
     3661 GTGAGCTGGG TAGCTCAGAC TGTCCTCCCA CCTCGTCCAG GTGCATCAAC ACCGAAGGTG 
     3721 GCTATGTCTG CCAATGCTCA GAAGGCTACG AGGGAGATGG GATCTACTGT CTCGACGTTG 
     3781 ATGAGTGCCA GCAGGGGTCG CACGGCTGCA GCGAGAATGC CACCTGCACC AACACGGAGG 
     3841 GAGGCTACAA CTGCACCTGT GCAGGCTGCC CATCAGCACC TGGACTGCCT TGCCCTGACT 
     3901 CTACCTCACC CTCTCTCCTT GGAAAAGATG GCTGCCACTG GGTCCGAAAC AGTAACACAG 
     3961 GATGCCCGCC GTCGTACGAT GGGTACTGCC TCAATGGTGG CGTGTGCATG TATGTTGAAT 
     4021 CCGTGGACCG CTACGTGTGC AACTGTGTCA TTGGCTATAT TGGAGAACGA TGTCAGCACC 
     4081 GAGACTTACG TTGGTGGAAG CTGCGCCATG CTGACTACGG GCAGAGGCAC GACATCACTG 
     4141 TGGTGTCTGT CTGTGTGGTG GCGCTGGCCC TGCTGCTCCT CTTAGGGATG TGGGGGACTT 
     4201 ACTACTACAG GACTCGGAAG CAGCTATCAG AGAGCTCAAA GAAGCCTTCC GAAGAGTCAA 
     4261 GCAGCAACGT GAGCAGTAAC GGGCCTGACA GCAGCGGGGC TGGGGTGTCT TCTGGTCCCC 
     4321 AACCTTGGTT TGTGGTCCTA GAGGAACACC AACAGCCCAA GAATGGGCGT CTGCCTGCCG 
     4381 CTGGCACGAA CGGCGCAGTA GTAGAGGCTG GCCTGTCTTC CTCCCTGACG CGTACGCGGC 
     4441 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4501 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4561 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4621 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4681 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4741 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4801 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4861 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4921 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4981 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5041 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5101 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5161 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5221 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5281 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5341 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5461 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5521 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5581 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5641 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5761 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5821 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5881 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5941 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6061 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6121 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6181 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6241 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6301 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6361 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6421 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6481 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6541 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6601 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6661 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6721 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6781 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6841 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6901 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6961 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7021 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7081 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7141 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7201 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7261 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7321 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7381 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7441 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7501 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7681 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7741 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7801 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7861 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7921 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7981 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8041 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8101 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8161 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8221 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//