LOCUS       RR201086                8655 bp    DNA     circular  SYN 
DEFINITION  Ehmt2 (Myc-DDK-tagged ORF) - Rat euchromatic histone lysine N-methyltransferase 2 (Ehmt2), (10 ug)
ACCESSION   NM_212463
VERSION     NM_212463.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4812..4903 
                     /label="Myc-DDK"  
     misc_feature    4933..5518 
                     /label="PolyA signal"  
     misc_feature    4990..5009 
                     /label="Restriction Enzyme"  
     misc_feature    5794..6466 
                     /label="ColE1"  
     misc_feature    6896..7690 
                     /label="Kan/Neo"  
     misc_feature    7725..8027 
                     /label="SV40 ori"  
     misc_feature    8113..8590 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4809..4815 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4809 
                     /label="Ehmt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGGTCTG CCGAGAGGGA GGGGGCTGAT GCGGGCCCGG GGGCGGGGGC 
     1081 GTGCGGCCCC CACGGGCGGC CGCGGCCGGG GTCGCGGGGG CGCCCACCGT GGACGAGGTA 
     1141 GGCCCCGAAG CCTGCTCTCG CTGCCCAGGG CCCAGGCGTC TTGGGCCCCC CAGCTGCCTG 
     1201 CCGGGCTGAC CGGCCCTCCG GTCCCTTGTC TCCCCTCCCA GGGGGAGGCC CCCGCTGAGA 
     1261 TGGGGGCGCT GCTGCTGGAG AAGGAGCCCC GAGGAGCCGC CGAGAGAGTT CATGGCTCTT 
     1321 TGGGGGACAC CTCTCATAGT GAGGAGACCC TTCCCAAGGC CAACCCCGAC TCCTTGGAGC 
     1381 CTACCGGCCC CTCCTCTCCG GCCTCTGTCA CTGTCACCGT CGGCGATGAG GGGGCTGACA 
     1441 CCCCTGTCGG GGCCACACCG CTCATCGGGG AGGAACCCGA GAACCTGGAG GGAGATGGGG 
     1501 GTCGAATCCT GCTGGGCCAT GCCACAAAGT CATTCCCCTC TTCCCCCAGC AAGGGGGGTG 
     1561 CCTGTCCCAG TCGGGCCAAA ATGTCAATGA CAGGGGCAGG AAAGTCGCCC CCCTCGGTCC 
     1621 AGAGTTTGGC CATGAGGCTG TTGAGCATGC CTGGGGCCCA GGGAGCTGCA ACTGCTGGGC 
     1681 CTGAGCCCCC TCCGGCAACA ACTGCCGGCC AGGAGGGGCA GCCCAAAGTA CACCGAGCTC 
     1741 GGAAAACCAT GTCCAAGCCT AGCAACGGAC AGCCTCCAGT CCCTGAGAAG CGGCCCCCTG 
     1801 AAGTCCAGCA TTTCCGCATG AGTGACGACA TGCATCTGGG AAAGGTGACT TCAGATGTGG 
     1861 CCAAACGGAG GAAGTTGACC TCGGGCAGCC TGTCAGAGGA CTTGGGCTCT GCTGGGGGCT 
     1921 CAGGAGAAGT GATCCTGGAG AAGGGAGAGC CCAGGCCTCT GGAGGAGTGG GAGACGGTGG 
     1981 TGGGCGATGA CTTCAGCCTG TACTATGACG CGTACTCTGT GGATGAGCGG GTGGACTCTG 
     2041 ACAGCAAGTC TGAAGTCGAA GCTCTAGCTG AACAGTTGAG TGAAGAGGAG GAGGAGGAAG 
     2101 AGGAGGAAGA AGAAGAGGAA GAGGAGGAAG AGGAGGAGGA GGAAGAGGAG GAGGAGGATG 
     2161 AGGAGTCGGG CAACCAGTCA GACAGGAGTG GCTCTAGCGG CCGACGCAAG GCCAAGAAGA 
     2221 AATGGCGGAA AGACAGCCCA TGGGTGAAGC CATCCAGGAA ACGGCGGAAG CGAGAGCCTC 
     2281 CGAGGGCCAA GGAGCCCAGA GGAGTGAATG GTGTGGGTTC CTCAGGGCCC AGTGAGTACA 
     2341 TGGAGGTTCC TCTGGGGTCC CTGGAGCTGC CCAGCGAGGG GACCCTCTCC CCCAACCACG 
     2401 CTGGGGTCTC CAATGACACG TCTTCGCTGG AGACGGAGCG TGGGTTTGAG GAGCTGCCCC 
     2461 TCTGCAGCTG CCGCATGGAG GCGCCCAAGA TTGACCGCAT CAGCGAGAGA GCAGGACACA 
     2521 AGTGTATGGC CACCGAAAGT GTGGACGGAG AGCTGCTGGG TTGCAATGCC GCCATCTTGA 
     2581 AGCGGGAGAC CATGCGGCCG TCTAGCCGTG TGGCGCTGAT GGTACTGTGT GAGGCCCATC 
     2641 GAGCCCGCAT GGTCAAGCAC CATTGCTGCC CAGGCTGTGG CTACTTCTGC ACGGCGGGCA 
     2701 CCTTCCTGGA GTGTCACCCC GACTTCCGTG TAGCTCACCG CTTCCATAAG GCCTGCGTGT 
     2761 CCCAGCTCAA TGGGATGGTT TTCTGTCCCC ACTGTGGGGA GGACGCCTCA GAGGCCCAGG 
     2821 AGGTGACCAT CCCTCGGGGC GATGGGGGAA CACCCCCGGT TGGCACTGTG GCGCCTGCCC 
     2881 CGCCACCCCT GGCACACGAC GCCCCAGGGC GAGCGGATAC CTCCCAGCCC AGTGCCCGAA 
     2941 TGCGAGGACA CGGAGAGCCA AGGCGCCCAC CCTGTGATCC CCTGGCCGAC ACCATTGACA 
     3001 GCTCGGGGCC TTCTCTGACT CTGCCTAATG GCGGCTGCCT CTCAGCTGTG GGTCTGCCCC 
     3061 CAGGGCCAGG CCGGGAAGCC CTGGAAAAAG CCTTGGTCAT CCAGGAGTCC GAGAGGAGGA 
     3121 AGAAGCTGCG GTTCCACCCG CGGCAGCTCT ACCTGTCGGT GAAGCAGGGG GAGCTGCAGA 
     3181 AGGTGATCCT CATGCTGCTC GACAACCTGG ACCCCAACTT CCAGAGCGAC CAGCAGAGCA 
     3241 AGCGCACGCC CCTGCACGCA GCCGCCCAGA AGGGGTCCGT GGAGATCTGT CACGTGCTGC 
     3301 TACAGGCAGG AGCCAACATC AACGCCGTGG ATAAGCAGCA GCGCACCCCG CTGATGGAGG 
     3361 CCGTGGTGAA CAACCACCTG GAGGTGGCTC GCTACATGGT GCAGCTGGGC GGCTGTGTGT 
     3421 ACAGCAAGGA AGAGGATGGC TCTACCTGTC TCCATCATGC GGCCAAAATT GGGAACTTGG 
     3481 AAATGGTCAG CCTGCTGCTG AGCACAGGGC AGGTGGACGT CAATGCCCAG GACAGTGGGG 
     3541 GCTGGACACC CATCATCTGG GCAGCCGAGC ACAAGCACAT CGACGTGATC CGTATGCTGC 
     3601 TGACCCGGGG CGCCGACGTC ACCCTTACCG ACAACGAGGA AAACATCTGC CTGCACTGGG 
     3661 CTTCCTTCAC GGGTAGTGCC GCTATTGCCG AGGTCCTCCT GAATGCCCAG TGCGACCTCC 
     3721 ATGCTGTCAA CTACCACGGG GACACGCCCC TGCACATAGC CGCCAGGGAG AGCTACCATG 
     3781 ACTGCGTTCT GTTGTTCCTG TCCCGTGGAG CCAACCCTGA GCTTCGGAAC AAAGAAGGGG 
     3841 ACACGGCGTG GGATCTGACC CCAGAGCGCT CCGATGTGTG GTTTGCACTG CAGCTCAATC 
     3901 GCAAGCTTAG GCTCGGGGTA GGGAACCGGG CTGTCCGCAC CGAGAAGATC ATCTGCCGGG 
     3961 ATGTAGCCCG AGGCTATGAG AATGTGCCCA TTCCCTGTGT CAATGGCGTG GATGGGGAGC 
     4021 CATGCCCAGA GGACTATAAG TACATCTCCG AGAACTGTGA AACGTCCACC ATGAACATCG 
     4081 ACCGAAACAT CACTCACCTG CAGCACTGCA CGTGTGTTGA CGACTGCTCC AGCTCCAACT 
     4141 GCCTGTGTGG CCAGCTAAGC ATCCGATGCT GGTATGATAA GGACGGGCGG CTGCTCCAGG 
     4201 AGTTTAACAA GATCGAGCCC CCCCTGATCT TTGAGTGTAA CCAGGCATGC TCCTGCTGGA 
     4261 GAAGCTGCAA GAACCGCGTG GTGCAGAGCG GCATCAAGGT GCGGCTGCAG CTCTACCGGA 
     4321 CGGCCAAGAT GGGCTGGGGG GTCCGTGCCT TGCAGACCAT TCCCCAGGGC ACGTTCATCT 
     4381 GCGAGTACGT CGGGGAGCTG ATCTCCGACG CCGAGGCAGA CGTGAGAGAG GATGATTCTT 
     4441 ACCTCTTCGA CTTAGACAAC AAGGATGGTG AGGTCTACTG CATTGATGCC CGTTACTATG 
     4501 GCAACATCAG CCGCTTCATT AACCACCTGT GCGACCCCAA CATCATCCCT GTTCGGGTCT 
     4561 TCATGCTGCA TCAAGACCTA CGGTTCCCTC GCATCGCCTT CTTCAGCTCC AGGGACATCC 
     4621 GGACCGGGGA GGAGCTGGGG TTTGACTACG GCGACCGGTT CTGGGATATC AAGAGCAAGT 
     4681 ATTTCACCTG CCAGTGTGGC TCTGAGAAGT GCAAGCATTC AGCTGAGGCC ATCGCCCTGG 
     4741 AGCAGAGCCG CCTGGCCCGT CTGGACCCCC ACCCGGAGCT GCTCCCTGAC CTCAGCTCCC 
     4801 TGCCCCCCAT CAACACCCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4861 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4921 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4981 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5041 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5101 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5161 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5221 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5281 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5341 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5401 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5461 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5521 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5581 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5641 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5701 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6481 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6541 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6601 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6661 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6721 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6781 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6841 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6901 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6961 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7021 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7081 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7141 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7201 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7261 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7321 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7381 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7441 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7501 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7561 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7621 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7681 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7741 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7801 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7861 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8221 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8281 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8341 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8401 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8461 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8521 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8581 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8641 TTTAACGCGA ATTTT
//