LOCUS       RR200937                8646 bp    DNA     circular  SYN 
DEFINITION  L1cam (Myc-DDK-tagged ORF) - Rat L1 cell adhesion molecule (L1cam), (10 ug)
ACCESSION   NM_017345
VERSION     NM_017345.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4803..4894 
                     /label="Myc-DDK"  
     misc_feature    4924..5509 
                     /label="PolyA signal"  
     misc_feature    4981..5000 
                     /label="Restriction Enzyme"  
     misc_feature    5785..6457 
                     /label="ColE1"  
     misc_feature    6887..7681 
                     /label="Kan/Neo"  
     misc_feature    7716..8018 
                     /label="SV40 ori"  
     misc_feature    8104..8581 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4785..4791 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4785 
                     /label="L1cam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCATGATG CTGTGGTACG TGTTGCCTCT TCTCCTCTGC AGCCCCTGCC 
     1081 TGCTCATACA GATTCCTGAT GAATATAAAG GACACCATGT ACTGGAGCCA CCTGTCATCA 
     1141 CAGAACAGTC TCCACGGCGC CTGGTTGTCT TCCCAACAGA TGACATAAGC CTCAAATGTG 
     1201 AAGCCAGAGG CAGACCCCAA GTGGAGTTCC GCTGGACGAA AGATGGCATC CACTTCAAAC 
     1261 CTAAGGAAGA ATTGGGTGTA GTGGTACACG AGGCACCCTA TTCTGGCTCC TTCACCATCG 
     1321 AAGGCAACAA CAGCTTTGCC CAGAGGTTTC AGGGCATCTA TCGCTGCTAT GCCAGCAATA 
     1381 ATCTAGGAAC TGCCATGTCG CATGAGATCC AGCTCGTGGC TGAGGGTGCC CCCAAATGGC 
     1441 CGAAGGAGAC TGTAAAACCC GTGGAAGTGG AGGAAGGAGA ATCAGTAGTT CTACCTTGCA 
     1501 ATCCTCCACC CAGTGCAGCC CCACTTAGGA TCTACTGGAT GAACAGCAAG ATTTTGCACA 
     1561 TCAAACAAGA TGAGCGGGTG TCCATGGGCC AGAACGGAGA CCTATATTTT GCCAATGTGC 
     1621 TTACCTCAGA CAATCATTCA GACTACATCT GCAATGCCCA CTTCCCTGGC ACCCGGACCA 
     1681 TCATTCAAAA GGAACCTATT GACCTCCGGG TCAAGCCCAC CAACAGCATG ATTGACCGGA 
     1741 AGCCACGCCT GCTCTTCCCC ACAAACTCCA GCAGTCACCT CGTGGCCTTG CAGGGCCAGT 
     1801 CATTAATCCT GGAGTGCATT GCTGAGGGAT TCCCTACACC CACCATCAAG TGGCTGCACC 
     1861 CCAGTGACCC TATGCCAACA GACCGTGTTA TCTACCAGAA CCATAACAAG ACACTGCAGC 
     1921 TCCTCAATGT GGGCGAGGAA GATGATGGCG AGTATACCTG CCTTGCTGAG AACTCACTGG 
     1981 GCAGTGCTCG GCATGCCTAC TATGTCACTG TGGAAGCTGC CCCATACTGG CTGCAGAAGC 
     2041 CCCAGAGTCA TTTGTATGGG CCAGGAGAGA CTGCCCGCCT AGACTGCCAA GTCCAGGGCA 
     2101 GGCCCCAACC AGAGGTCACC TGGAGAATCA ACGGAATGTC TATAGAGAAG GTGAACAAGG 
     2161 ACCAGAAGTA CCGGATTGAG CAGGGGTCTT TGATCCTGAG TAATGTGCAA CCAAGTGACA 
     2221 CAATGGTGAC CCAGTGTGAA GCTCGCAACC AGCATGGGCT CCTACTAGCC AATGCCTATA 
     2281 TCTATGTTGT CCAGCTGCCA GCCAGGATCC TAACAAAAGA CAATCAGACA TACATGGCAG 
     2341 TAGAGGGCAG TACTGCTTAC TTGCTGTGCA AAGCCTTTGG AGCTCCTGTT CCCAGTGTCC 
     2401 AGTGGCTGGA TGAGGAAGGA ACCACAGTGC TTCAGGATGA AAGATTTTTC CCCTATGCCA 
     2461 ATGGACACCT GGGCATCAGA GATCTCCAGG CCAATGACAC TGGACGCTAT TTCTGCCAGG 
     2521 CTGCCAATGA CCAGAACAAT GTGACCATTT TGGCTAACCT ACAGGTTAAA GAAGCAACCC 
     2581 AGATCACACA AGGACCCCGG AGCACAATTG AGAAGAAAGG TGCAAGGGTG ACATTCACGT 
     2641 GCCAGGCCTC CTTTGACCCC TCTTTACAAG CCAGCATCAC TTGGCGTGGA GATGGGAGAG 
     2701 ACCTCCAGGA ACGTGGAGAC AGTGACAAGT ATTTCATAGA AGATGGGCAA CTTGTCATCA 
     2761 AGAGCCTGGA CTACAGTGAC CAGGGAGACT ACAGTTGTGT GGCCAGCACT GAACTGGATG 
     2821 AGGTGGAGAG CAGGGCACAA CTCTTAGTGG TGGGAAGCCC TGGGCCAGTG CCTCACCTGG 
     2881 AGCTGTCCGA CCGCCACTTG CTGAAGCAGA GCCAGGTGCA CTTGTCTTGG AGCCCTGCTG 
     2941 AAGACCACAA CTCTCCCATT GAGAAATATG ACATTGAATT TGAGGACAAG GAAATGGCTC 
     3001 CTGAGAAATG GTTCAGTCTA GGCAAGGTGC CAGGAAATCA GACCTCTACT ACCCTCAAGC 
     3061 TGTCCCCCTA TGTCCACTAC ACCTTTCGGG TCACTGCCAT TAACAAATAT GGTCCTGGAG 
     3121 AACCCAGCCC TGTCTCTGAG ACTGTAGTCA CACCTGAGGC AGCCCCAGAG AAGAACCCTG 
     3181 TGGATGTGAG AGGGGAAGGA AATGAGACCA ACAATATGGT CATCACATGG AAGCCCCTTC 
     3241 GGTGGATGGA TTGGAATGCC CCCCAGATTC AGTACCGTGT ACAGTGGCGA CCACTGGGCA 
     3301 AACAAGAGAC CTGGAAGGAA CAGACCGTGA GCGACCCCTT CCTGGTGGTG TCTAACACTT 
     3361 CCACATTTGT GCCTTATGAG ATCAAAGTCC AGGCAGTGAA CAACCAGGGG AAGGGCCCTG 
     3421 AGCCCCAGGT CACCATTGGC TATTCAGGGG AAGACTACCC CCAGGTGAGC CCTGAGCTTG 
     3481 AAGACATCAC AATCTTCAAC TCAAGCACTG TGCTGGTCAG GTGGAGGCCT GTGGACTTGG 
     3541 CCCAGGTTAA GGGCCACCTC AGGGGATACA ATGTAACGTA CTGGTGGAAG GGCAGTCAGA 
     3601 GAAAGCACAG CAAGAGGCAT GTCCACAAAA GTCACATGGT GGTACCTGCG AACACCACCA 
     3661 GTGCCATCCT CAGTGGTTTG CGTCCTTACA GCTCTTATCA TGTAGAGGTA CAGGCCTTTA 
     3721 ATGGGCGGGG CTTAGGGCCT GCAAGTGAAT GGACCTTCAG CACCCCAGAG GGAGTGCCTG 
     3781 GCCACCCTGA GGCATTACAT CTGGAGTGCC AGTCGGACAC TAGCCTGCTA CTGCACTGGC 
     3841 AGCCACCACT CAGCCACAAT GGAGTGCTCA CTGGCTACCT GCTCTCTTAC CATCCCTTGG 
     3901 ATGGGGAAAG CAAAGAGCAG TTGTTCTTCA ACCTTTCGGA CCCAGAGCTC CGGACTCATA 
     3961 ATCTCACCAA CCTCAACCCT GATCTACAGT ACCGCTTCCA GCTTCAGGCC ACCACCCATC 
     4021 AGGGTCCTGG TGAGGCCATT GTGCGTGAAG GAGGCACTAT GGCCCTATTT GGCAAGCCAG 
     4081 ATTTTGGCAA CATTTCAGTC ACAGCAGGTG AAAACTACAG TGTGGTCTCC TGGGTCCCTC 
     4141 GGGAGGGCCA GTGCAATTTC AGGTTCCACA TCCTGTTCAA AGCCTTGCCA GAAGGGAAAG 
     4201 TGAGCCCTGA TCACCAGCCT CAGCCTCAAT ATGTGAGCTA CAATCAGAGC TCCTACACAC 
     4261 AGTGGGACCT ACAGCCTGAC ACCAAATATG AGATCCACCT GATGAGGGAG AAGGTCCTCT 
     4321 TGCACCATCT GGCTGTGAAG ACTAATGGCA CTGGCCCCGT GCGAGTTTCT ACTACAGGTA 
     4381 GCTTTGCCTC CGAGGGCTGG TTCATCGCCT TTGTCAGTGC TATCATTCTC TTGCTCCTCA 
     4441 TCCTGCTCAT CCTCTGCTTC ATCAAACGCA GCAAGGGCGG CAAATATTCA GTGAAGGACA 
     4501 AGGAGGACAC TCAGGTAGAT TCCGAGGCCC GGCCCATGAA AGACGAGACC TTCGGCGAGT 
     4561 ACAGTGACAA TGAAGAGAAG GCCTTCGGCA GCAGCCAGCC ATCTCTCAAT GGAGACATCA 
     4621 AACCCCTAGG CAGTGATGAC AGTCTGGCTG ATTATGGGGG CAGTGTGGAT GTCCAGTTCA 
     4681 ATGAGGATGG CTCTTTCATC GGCCAATACA GTGGCAAAAA AGAGAAGGAG GCAGCGGGAG 
     4741 GCAATGACAG CTCAGGGGCT ACCTCTCCTA TCAATCCTGC AGTAGCCCTA GAAACGCGTA 
     4801 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4861 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4921 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4981 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5041 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5101 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5161 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5221 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5281 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5341 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5401 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5461 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5521 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5581 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5641 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5701 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5761 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5821 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5881 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5941 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6001 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6061 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6121 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6181 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6241 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6301 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6361 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6421 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6481 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6541 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6601 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6661 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6721 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6781 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6841 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6901 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6961 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7021 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7081 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7141 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7201 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7261 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7321 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7381 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7441 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7501 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7561 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7621 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7681 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7741 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7801 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7861 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8221 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8281 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8341 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8401 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8461 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8521 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8581 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8641 AATTTT
//