LOCUS       RR200737                6711 bp    DNA     circular  SYN 
DEFINITION  Mylk2 (Myc-DDK-tagged ORF) - Rat myosin light chain kinase 2 (Mylk2), (10 ug)
ACCESSION   NM_057209
VERSION     NM_057209.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2868..2959 
                     /label="Myc-DDK"  
     misc_feature    2989..3574 
                     /label="PolyA signal"  
     misc_feature    3046..3065 
                     /label="Restriction Enzyme"  
     misc_feature    3850..4522 
                     /label="ColE1"  
     misc_feature    4952..5746 
                     /label="Kan/Neo"  
     misc_feature    5781..6083 
                     /label="SV40 ori"  
     misc_feature    6169..6646 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2850..2856 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2850 
                     /label="Mylk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTACAGAA AACGGAGCAG TTGAGCTGGG AACCCAGAGT CTGTCAACAG 
     1081 ACCACCCACC TACAGACGCA GCAGGTGATG GATCTCCAGC TTCAGAGAAA GAACCCAGCC 
     1141 TCCCAGACAC AGAGAAAGAC CTGGGCCCCA CTAACACGAA GAAAGACCCT GGCGCGCCGG 
     1201 ATCCAAAGAA AAATCCAGAT CCACCCTCCC TGAAGAAAAC TCCCGAAGCC CCTGGTCCAG 
     1261 AGAAAAAGGG TGACTCGGCC CCAGCTTCAG CCAGCAACCA GGGCCCTTCA GGAGAGGGTG 
     1321 ATGGAGGTGG GGGCCCTGCT GAAGGCGGTA CGGGGCCTCC TGCAGTCTTG CCCCAGCCCA 
     1381 CTGCCACAGC TGATGCCAGC ATCCAGAAGC TGGACGCCAC GCAAGCGCCC TCGGGCAACC 
     1441 AGGAATCTGG AGAGGCCAAG GCAGGCAAGA AGGCAGCAGA ATGCCGAGAA GCAGGACGAA 
     1501 GGGGCTCACC CGCCTTTCTT CACAGCCCTA GCTGCCCCGC GATCATCTCC TGTTCTGAGA 
     1561 AGACACTGGC CATGAAGCCC TTAAGCGAGA CAACAGAGCT CATCTTTGCA GGGGTGTCTG 
     1621 AAACCCCTGA TCCCCAAGAT CCTGGTCCAG CTAAGGACGA AGGAGGGACG AACACGCTGG 
     1681 CAGACGGGAA AGAAGAGGCA GAGGCAGGCC AGGCTGAGCA GGCTAAGGTG CAAGGGGACA 
     1741 CCTCCCAGAG GATCGGGTTC CAGGCTGTTC CCTCAGAGAG AGCAGAGGTG GGGCAGGCCC 
     1801 TCTGTCTCAC GGCCAAGGAG GAAGACTGCT TCCAAATTCT GGATGATTGT CCACCACCCC 
     1861 CAGCCCCCTT CCCACACAGG ATCGTGGAGC TGAGGACCGG AAATGTCAGC AGTGAATTCA 
     1921 GCATGAACTC AAAGGAGGCA TTGGGAGGTG GCAAGTTTGG AGCTGTGTGC ACCTGCACGG 
     1981 AGAGATCCAC AGGCCTCAAG CTGGCAGCCA AGGTCATCAA GAAACAGACC CCCAAGGACA 
     2041 AGGAAATGGT GTTGCTGGAG ATCGAGGTCA TGAACCAGCT GAACCACCGC AATTTGATCC 
     2101 AGCTGTACTC AGCCATTGAG ACCTCTCACG AGATCATCCT GTTCATGGAG TACATTGAGG 
     2161 GCGGCGAGCT CTTTGAGAGG ATCGTGGATG AGGACTACCA ACTGACGGAA GTGGATACCA 
     2221 TGGTGTTCGT CAGGCAGATC TGCGACGGGA TCCTCTTCAT GCACAAGATG AGGGTCCTGC 
     2281 ACTTGGACCT CAAGCCAGAG AACATCCTGT GTGTGAATAC CACCGGGCAC TTGGTGAAGA 
     2341 TAATTGACTT CGGCCTGGCA AGAAGGTATA ACCCCAATGA GAAGCTGAAG GTGAATTTTG 
     2401 GGACCCCGGA GTTCCTGTCA CCAGAGGTGG TGAACTATGA CCAGATCTCT GACAAGACGG 
     2461 ACATGTGGAG TCTTGGGGTG ATCACCTACA TGCTACTGAG TGGCCTCTCT CCCTTCCTGG 
     2521 GCGACGATGA CACGGAGACC CTAAACAACG TCCTGTCTGC CAACTGGTAC TTTGATGAGG 
     2581 AGACTTTTGA AGCTGTGTCA GACGAGGCCA AAGACTTCGT TTCCAATCTT ATCACGAAGG 
     2641 ACCAGAGTGC CAGAATGAGT GCTGAGCAGT GCCTCGCCCA CCCCTGGCTC AACAACCTGG 
     2701 CAGAAAAAGC CAAGCGCTGC AACCGTCGTC TCAAGTCCCA GATCCTGCTT AAGAAATACC 
     2761 TCATGAAGAG GCGCTGGAAG AAAAACTTCA TTGCTGTCAG CGCACGCAAT CGCTTCAAGA 
     2821 AGATCAGTAG CTCGGGGGCA CTGATGGCTT TGGGGGTCAC GCGTACGCGG CCGCTCGAGC 
     2881 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     2941 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3001 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3061 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3121 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3181 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3241 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3301 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3361 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3421 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3481 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3541 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3601 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3661 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3721 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3781 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     3841 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     3901 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     3961 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4021 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4081 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4141 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4201 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4261 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4321 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4381 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4441 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4501 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4561 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4621 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4681 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4741 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4801 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     4861 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     4921 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     4981 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5041 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5101 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5161 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5221 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5281 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5341 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5401 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5461 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5521 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5581 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5641 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5701 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5761 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     5821 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     5881 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     5941 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6001 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6061 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6121 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6181 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6241 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6301 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6361 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6421 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6481 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6541 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6601 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6661 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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