LOCUS       RR200594                7200 bp    DNA     circular  SYN 
DEFINITION  Pank4 (Myc-DDK-tagged ORF) - Rat pantothenate kinase 4 (Pank4), (10 ug)
ACCESSION   NM_133531
VERSION     NM_133531.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3357..3448 
                     /label="Myc-DDK"  
     misc_feature    3478..4063 
                     /label="PolyA signal"  
     misc_feature    3535..3554 
                     /label="Restriction Enzyme"  
     misc_feature    4339..5011 
                     /label="ColE1"  
     misc_feature    5441..6235 
                     /label="Kan/Neo"  
     misc_feature    6270..6572 
                     /label="SV40 ori"  
     misc_feature    6658..7135 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3339..3345 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3339 
                     /label="Pank4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTGC CGGGCGAGTG GCGGCGGGAG CGGCGGGGAC AGTCTGGACA 
     1081 AGAGCATCAC GCTGCCCCCC GACGAGATCT TCCGAAACCT GGAGAACGCC AAGCGCTTCG 
     1141 CCATTGATAT AGGTGGATCA CTGACCAAGT TGGCATACTA TTCCACAGTA CAGCACAAAG 
     1201 TGGCCAAAGT GAGGTCTTTT GACCACCCAG GAAAGGACGC AGAACAGGAC CATGAGCCGC 
     1261 CCTATGAGAT CTCAGTTCAG GAGGAGATCA CAGCTCGTCT GCATTTCATC AAGTTTGAGA 
     1321 ACACCTACAT GGAAGCCTGC CTGGACTTTA TCAGAGACCA CCTTGTCAAC ACTGAGACCA 
     1381 AGGTCATCCA GGCCACAGGG GGTGGAGCCT ACAAGTTCAA GGACCTCATC GAGGAGAAGC 
     1441 TGCGTCTGAA GGTGGACAAA GAGGATGTGA TGACCTGCTT GATTAAGGGG TGCAACTTCG 
     1501 TGCTGAAGAA CATCCCACAT GAGGCCTTCA TGTACCAGAA AGACTCAGAC CCAGAGTTTC 
     1561 GGTTTCAGAC AAATCACCCG AACATCTTCC CCTACCTCCT AGTCAACATT GGCTCTGGAG 
     1621 TCTCCATCGT GAAGGTGGAG ACAGAGGACC GGTTCGAGTG GATTGGTGGG AGCTCCATTG 
     1681 GAGGAGGCAC CTTCTGGGGG CTTGGGGCTC TGCTCACCAA AACAAAGAAG TTTGATGAGC 
     1741 TGCTGCAGCT GGCTTCCAGA GGCCGGCACG CCAATGTTGA CATGCTGGTA CAAGACATCT 
     1801 ATGGAGGGGC CCACCAGACC CTGGGGCTGA GCGGCAATCT CATCGCAAGC AGTTTTGGGA 
     1861 AGTCAGCCAC TGCTGACAGA GAGTTCTCCA AAGAAGACAT GGCCAAGAGC CTGCTGCACA 
     1921 TGATCAGCAA TGACATTGGA CAGCTCGCCT GTCTCTACGC CAAGCTTCAT GGCCTAGACA 
     1981 GGGTCTACTT TGGGGGTTTC TTCATCCGGG GTCACCCTGT GACCATGCGC ACCATCACCT 
     2041 ACAGCATTAA CTTCTTCTCT AAGGGTGAAG TCCAGGCACT CTTCCTGAGA CATGAAGGCT 
     2101 ACCTGGGAGC CATCGGGGCA TTTTTGAAAG GAGCCGAGCA AGATAATCCT AACCAGTACA 
     2161 GCTGGGGTGA GAACTACGCG GCCAGCTCCG GGCTGATGAG CACGGCGCCG GAGCTGTGCC 
     2221 CGACACAGCG GGCAAGGAGC GGCACATTTG ACCTGCTGGA AATGGACCGC CTGGAGAGAC 
     2281 CCCTGGTCAA CCTGCCCCTC CTCCTGGACC CATCCTCCTA TGTGCCCGAC ACGGTAGACC 
     2341 TCACTGACGA TGCTCTGGCC CGACAGTACT GGCTCACATG CTTTGAGGAG GCTCTGGATG 
     2401 GGGTGGTGAA GCGAGCTGTG GCCAGCCAGC CAGAATCCGT GGATGCAGCT GAGAGGGCAG 
     2461 AGAAGTTCCG TCAGAAGTAC TGGGGCAAAC TTCAGACTCT GAGGCACCAG CCCTTTGCTT 
     2521 ATGGGACGCT GACTGTGCGC AGCCTGTTGG ACACAAGAGA GCACTGTTTG AATGAGTTCA 
     2581 ACTTCCCAGA CCCCTACTCC AAGGTGAAGC AGAAAGAAAA CGGCCTCGCG CTAAAGTGCT 
     2641 TTCAGAGCGT GACTCGCTCG CTGGACTCAC TAGGCTGGGA GGAGCGGCAG CTGGCCCTGG 
     2701 TGAAGGGGCT GTTAGCTGGG AATGTCTTTG ACTGGGGAGC CAAGGCTGTG TCTGACGTCC 
     2761 TGGAATCGGA CCCCCAGTTT GGGTTTGAAG AAGCAAAGAG AAAATTGCAA GAACGGCCCT 
     2821 GGCTGGTGGA TTCCTACACC AAGTGGCTCC AGAGGTTAAA GGGGCCCCCT CATAAATGTG 
     2881 CCTTAATTTT CGCAGATAAC AGTGGAATAG ACATCATTTT GGGAGTCTTC CCCTTTGTCA 
     2941 GGGAGCTACT CTGTAGAGGG ATAGAGGTCA TCTTGGCATG CAACTCAGGC CCTGCCCTGA 
     3001 ACGATGTGAC CTACAGTGAG TCTCTCATTG TGGCAGAACG CATTGCAGCC ATGGACCCCA 
     3061 TCATCTGCAC TGCACTCAGA GAAGACAGGC TACTGCTGGT GCAGACCGGT TCCAGTCCCC 
     3121 CATGCCTAGA TCTCAGCCGC CTAGATAAGG GACTGGCTGT GCTGGTGCGG GAGCGTGGTG 
     3181 CCGACCTGGT GGTCATCGAG GGAATGGGCC GTGCTGTCCA CACCAACTAC CATGCTTTGC 
     3241 TGCGATGTGA GAGTCTCAAG CTGGCCGTGG TGAAGAACGC CTGGCTGGCT GAGCGTCTGG 
     3301 GTGGCCAGCT CTTCAGTGTC ATCTTCAAGT ACGAGGTCCC AGCCGAGACG CGTACGCGGC 
     3361 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3421 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3481 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3541 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3601 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3661 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3721 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3781 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3841 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3901 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3961 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4021 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4081 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4141 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4201 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4261 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4321 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4381 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4441 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4501 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4561 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4621 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4681 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4741 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4801 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4861 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4921 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4981 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5041 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5101 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5161 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5221 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5281 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5341 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5401 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5461 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5521 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5581 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5641 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5701 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5761 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5821 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5881 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5941 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6001 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6061 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6121 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6181 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6241 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6301 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6361 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6421 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6481 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6541 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6601 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6661 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6721 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6781 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6841 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6901 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6961 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7021 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7081 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7141 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//