LOCUS       RR200573                8676 bp    DNA     circular  SYN 
DEFINITION  Plcg2 (Myc-DDK-tagged ORF) - Rat phospholipase C, gamma 2 (Plcg2), (10 ug)
ACCESSION   NM_017168
VERSION     NM_017168.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="Plcg2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACCATG GTCAACGTGG ACACCCTTCC AGAATATGAG AAGAGTCAGA 
     1081 TCAAGAGAGC CCTGGAGCTA GGGACGGTGA TGACCGTATT CAGTGCCCGC AAATCCACCC 
     1141 CAGAGCGGAG GACAGTACAG ATGATCATGG AGACCCGGCA GGTGGCATGG AGCAAGACAG 
     1201 CAGATAAGAT CGAAGGCTTC TTGGACATCA TGGAAATAAA GGAAATCCGG CCAGGGAAGA 
     1261 ACTCCAAGGA CTTTGAGCGA GCCAAGGCTG TCCGTCACAA GGCAGACTGC TGCTTCACCA 
     1321 TCTTCTACGG CACCCAGTTC GTGCTCAGCA CTCTCAGTCT GGCAACCGAC TCAAAGGAGG 
     1381 ATGCAGTGAA GTGGCTCTCT GGCTTGAAGA TCTTACACCA GGAAGCGATG AACGCATCCA 
     1441 CTCCCACAAT GATTGAGAGT TGGCTGCGGA AACAGATTTA TTCAGTAGAT CAAACCCGAA 
     1501 GAAACAGCAT CAGCCTCCGG GAGCTGAAGA CCATCTTGCC CCTGGTCAAC TTCAAAGTGA 
     1561 GCGGCATCAA GTTCCTCAAG GACAAGCTGG TGGAAATTGG CGCCCAGAAA GATGAGCTCA 
     1621 GTTTTGAACA GTTCCATCTC TTCTATAAAA AACTCATGTT TGAGCAGCAG AAGTCGATAC 
     1681 TTGACGAATT CAAAAAAGAC TCCTCCGTGT TCATCCTAGG GAACACAGAC CGGCCTGATG 
     1741 CCTCAGCCGT CTACCTGCAA GACTTCCAGA GGTTTCTTTT ACATGAACAG CAGGAGCTAT 
     1801 GGGCTCAGGA TCTGAACAAA GTCCGGGAGC GGATGACCAA GTTTATCGAT GACACAATGA 
     1861 GGGAGACCGC AGAGCCCTTC TTGTTTGTGG ATGAGTTCCT CACGTATCTG TTCTCACGGG 
     1921 AGAACAGCAT CTGGGACGAG AAGTACGATG CCGTGGACAT GCAGGACATG AACAACCCCT 
     1981 TGTCCCATTA CTGGATCTCC TCTTCCCACA ACACGTACCT CACTGGAGAC CAGCTGCGTA 
     2041 GCGAGTCCTC CACGGAAGCG TATATCCGCT GTCTGCGTGC TGGTTGTCGC TGCATCGAGT 
     2101 TGGATTGCTG GGATGGGCCT GACGGGAAAC CCATAATCTA TCACGGTTGG ACACGGACCA 
     2161 CCAAGATCAA GTTTGATGAT GTTGTTCAGG CCATCCGGGA TCATGCCTTT GTTACCTCCA 
     2221 GCTTTCCCGT GATTCTGTCT ATCGAGGAGC ACTGCAGTGT GGAGCAGCAA CGTCACATGG 
     2281 CCAAGGTCTT CAAGGAAGTG TTAGGAGACC TGCTGTTGAC GAAGCCCACG GAGGCCAGTG 
     2341 CTGACCAGCT GCCCTCACCC AGCCAGCTTC GTGAGAAGAT CATTATCAAG CATAAGAAGC 
     2401 TGGGCCCCCG AGGTGATGTC GATGTCAATG TGGAGGACAA GAAAGATGAG CACAAGACCC 
     2461 AGGGCGAGCT GTATATGTGG GACTCCATCG ACCAGAAATG GACTCGCCAC TACTGTGCTA 
     2521 TTGCGGACGC CAAGCTGTCC TTCAGTGACG ACATTGAACA GACTGTGGAA GAGGATCCGG 
     2581 TCCAGGACAC TCCCCCCACG GAGCTACATT TTGGGGAGAA ATGGTTCCAT AAGAAGGTGG 
     2641 AGAGCAGGAC CAGTGCGGAG AAGCTGCTGC AGGAGTACTG TGCCGAGACT GGGGCCAAGG 
     2701 ATGGCACCTT CCTGGTGCGG GAGAGCGAGA CCTTCCCCAA CGACTACACA CTCTCCTTCT 
     2761 GGCGGTCTGG CCGGGTGCAG CACTGCCGAA TCCGGTCTAC CATGGAGGGT GGGGTCATGA 
     2821 AGTACTACCT GACTGACAAC CTCACGTTCA ACAGCATCTA CGCCCTCATC CAGCACTACC 
     2881 GGGAGGCACA CCTGCGCTGT GCGGAGTTCG AGCTGCGGCT CACAGACCCG GTGCCCAACC 
     2941 CCAACCCACA TGAGTCCAAG CCGTGGTACT ATGACAGGCT GAGCAGGGGG GAAGCAGAGG 
     3001 ACATGTTGAT GCGGATTCCC CGAGATGGAG CCTTCCTCAT TCGGAAGCGG GAGGGGACCG 
     3061 ACTCTTACGC CATCACCTTC AGGGCCAGGG GCAAGGTGAA ACATTGCCGT ATCAACCGGG 
     3121 ACGGACGTCA CTTTGTGCTG GGGACCTCTG CCTACTTTGA GAGCCTGGTG GAGCTCGTCA 
     3181 GCTACTATGA GAAGCACGCA CTCTACCGCA AGATGAGGCT TCGCTACCCC GTGACCCCGG 
     3241 AGCTCCTGGA ACGATATAAT ATGGAAAGAG ACATCAACTC TCTCTATGAC GTCAGCAGGA 
     3301 TGTATGTGGA CCCAAGTGAG ATCAACCCTT CCATGCCTCA GAGGACTGTG AAAGCACTCT 
     3361 ATGACTACAA AGCCAAGCGC AGCGATGAGC TGACCTTCTG CCGAGGTGCC CTCATCCACA 
     3421 ACGTCTCCAA GGAGCCGGGA GGATGGTGGA AAGGGGACTA TGGGACCCGG ATCCAGCAGT 
     3481 ATTTCCCATC TAACTACGTT GAAGATATCT CGGCAGGCGA TGCTGAGGAG ATGGAAAAGC 
     3541 AGATTATTGA AGACAACCCC CTCGGGTCTC TCTGCAGAGG CATATTGGAT CTTAATACCT 
     3601 ACAATGTGGT GAAGGCCCCC CAGGGGAAAA ACCAGAAAGC CTTCGTCTTT ATCCTGGAGC 
     3661 CTAAGAAACA GGGGGACCCT CCTGTGGAGT TCGCGACTGA CCGTGTGGAG GAACTGTTTG 
     3721 AGTGGTTTCA GAGTATACGA GAGATCACTT GGAAGATCGA CACCAAGGAG AACAACATGA 
     3781 AGTACTGGGA GAGGAATCAG TCCATTGCTA TTGAGCTCTC TGACTTGGTT GTGTACTGCA 
     3841 AGCCGACTAG CAAAACCAAG GACCATTTGG AAAATCCTGA CTTCCGGGAA ATTCGCTCTT 
     3901 TTGTGGAGAC AAAGGCAGAC AGCATTGTCA GGCAAAAGCC CGTGGATCTA TTGCGGTACA 
     3961 ACCAGAAGGG CCTGACCCGT GTCTACCCCA AGGGACAGAG AGTTGACTCT TCCAACTACG 
     4021 ACCCCTTCCG TCTGTGGCTG TGTGGCTCCC AGATGGTGGC GCTCAATTTC CAGACTCCAG 
     4081 ACAAGTACAT GCAGATGAAC CATGCATTGT TTTCGCTCAA TGGGCGGACA GGTTACGTCC 
     4141 TGCAGCCCGA GAGCATGCGG TCTGAGAAGT ATGACCCGAT GCCCCCGGAG TCCCAGAGGA 
     4201 AGATCCTGAT GACACTCACT GTCAAGGTTC TTGGTGCACG CCACCTCCCT AAACTAGGGC 
     4261 GGAGTATCGC CTGTCCCTTT GTAGAGGTGG AAATCTGTGG GGCCGAGTAT GACAGCAACA 
     4321 AATTCAAGAC GACGGTTGTG AATGACAACG GTCTCAGCCC TGTCTGGGCT CCAACTCAGG 
     4381 AGAAGGTGAC ATTTGAAATT TATGACCCAA ACCTTGCGTT CCTACGCTTT CTGGTCTACG 
     4441 AAGAAGACAT GTTCAGTGAC CCCAACTTCC TGGCTCATGC CACATACCCC ATTAAAGGCA 
     4501 TCAAATCAGG ATTTAGATCA GTCCCTCTGA AGAACGGGTA CAGTGAAGAC ATCGAGCTGG 
     4561 CATCCCTCCT GGTTTTCTGT GAGATGCGGC CAGTCCTGGA GAGTGAAGAA GAACTCTATT 
     4621 CCTCCTGTCG CCAGCTGCGG AGGCGGCAGG AAGAGCTCAA CAACCAGCTC TTCCTGTATG 
     4681 ACACACACCA GAACCTGCGA GGAGCCAACC GGGATGCCCT GGTGAAGGAG TTCAATGTTA 
     4741 ACGAGAATCA GCTGCGGCTG TACCAGGAGA AGTGTAACCG GAGGCTGAGA GAGAAGAGAG 
     4801 TGAGTAACAG CAGGTTCTAC TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//