LOCUS       RR200532                8154 bp    DNA     circular  SYN 
DEFINITION  Acly (Myc-DDK-tagged ORF) - Rat ATP citrate lyase (Acly), transcript variant 2, (10 ug)
ACCESSION   NM_001111095
VERSION     NM_001111095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="Acly"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGCCAAG GCAATTTCAG AGCAGACCGG CAAAGAACTC CTTTACAAGT 
     1081 ACATCTGTAC CACCTCAGCC ATCCAGAACC GGTTCAAGTA TGCCCGGGTT ACTCCCGACA 
     1141 CAGACTGGGC CCATCTCCTG CAGGACCACC CCTGGCTGCT TAGCCAGAGC TTGGTAGTCA 
     1201 AGCCGGACCA GCTGATCAAA CGTCGAGGAA AGCTTGGTCT AGTCGGGGTC AACCTCTCTC 
     1261 TGGATGGAGT CAAATCCTGG CTGAAACCTC GACTGGGACA TGAGGCCACC GTCGGCAAGG 
     1321 CCAAAGGCTT CCTCAAGAAC TTTCTGATTG AGCCCTTCGT CCCCCACAGT CAGGCGGAGG 
     1381 AGTTCTACGT GTGCATCTAT GCTACCCGGG AAGGAGACTA CGTCCTGTTC CACCATGAAG 
     1441 GGGGTGTGGA TGTGGGCGAT GTGGACACCA AAGCCCAGAA GCTGCTTGTG GGTGTGGACG 
     1501 AGAAACTGAA CGCTGAAGAC ATTAAGAGAC ACCTGTTGGT CCACGCCCCC GAAGACAAGA 
     1561 AAGAAATCCT GGCCAGCTTC ATCTCCGGCC TATTCAATTT CTACGAAGAT CTTTACTTCA 
     1621 CCTACCTTGA GATCAACCCC CTTGTGGTGA CCAAAGATGG TGTCTACATC CTTGACCTGG 
     1681 CGGCCAAGGT GGACGCCACT GCTGACTACA TCTGCAAAGT CAAGTGGGGT GATATAGAGT 
     1741 TCCCTCCCCC CTTTGGGCGT GAGGCATACC CAGAGGAAGC CTACATTGCA GACCTGGATG 
     1801 CCAAAAGTGG GGCGAGCTTG AAGCTGACCT TGCTGAACCC CAAGGGGCGG ATCTGGACCA 
     1861 TGGTTGCCGG GGGTGGCGCC TCTGTCGTGT ACAGTGATAC CATCTGTGAT CTTGGAGGTG 
     1921 TCAACGAACT GGCGAATTAC GGGGAGTACT CTGGTGCCCC CAGTGAACAA CAGACCTATG 
     1981 ACTACGCCAA GACCATCCTC TCACTTATGA CTCGAGAGAA GCACCCGGAT GGCAAGATCC 
     2041 TCATCATTGG AGGCAGCATT GCAAACTTCA CCAACGTGGC CGCCACCTTC AAGGGCATTG 
     2101 TGAGAGCAAT TCGAGATTAC CAGGGTCCCC TGAAGGAGCA CGAGGTCACC ATCTTTGTTC 
     2161 GAAGAGGTGG CCCGAACTAT CAAGAGGGAT TACGAGTGAT GGGAGAAGTT GGGAAGACCA 
     2221 CTGGAATCCC CATCCATGTC TTTGGCACAG AAACTCACAT GACGGCCATT GTGGGCATGG 
     2281 CCCTGGGCCA CCGGCCCATT CCCAACCAGC CACCCACAGC GGCTCACACT GCCAACTTCC 
     2341 TCCTTAATGC CAGTGGGAGC ACATCGACAC CAGCACCCAG CAGGACAGCG TCTTTTTCCG 
     2401 AGTCCAGAGC TGACGAGGTG GCCCCTGCAA AGAAAGCCAA GCCAGCCATG CCCCAAGGAA 
     2461 AGAGTGCCAC CCTCTTCAGC CGACATACCA AGGCTATCGT GTGGGGCATG CAGACCCGGG 
     2521 CTGTGCAAGG CATGCTGGAC TTTGACTACG TGTGCTCCCG AGATGAGCCT TCAGTGGCTG 
     2581 CTATGGTCTA CCCGTTCACG GGGGATCATA AGCAGAAGTT TTACTGGGGA CACAAGGAAA 
     2641 TCCTGATCCC TGTCTTCAAG AACATGGCTG ACGCCATGAA AAAGCATCCG GAGGTAGACG 
     2701 TGCTGATCAA CTTTGCATCT CTGCGATCGG CTTATGACAG CACCATGGAG ACCATGAACT 
     2761 ATGCACAGAT CCGGACCATA GCCATCATAG CAGAAGGCAT CCCTGAGGCT CTCACACGGA 
     2821 AGCTCATCAA GAAGGCAGAC CAGAAGGGCG TGACCATCAT TGGGCCAGCC ACGGTTGGGG 
     2881 GCATCAAGCC TGGATGCTTT AAGATTGGGA ATACTGGTGG GATGCTGGAC AACATCCTGG 
     2941 CCTCCAAACT GTATCGCCCA GGCAGTGTGG CCTACGTCTC GCGTTCAGGA GGCATGTCTA 
     3001 ACGAACTCAA TAATATCATC TCTCGGACCA CAGATGGTGT CTACGAGGGT GTTGCCATCG 
     3061 GCGGGGACAG GTACCCTGGG TCCACATTCA TGGATCACGT GCTGCGTTAC CAAGACACTC 
     3121 CAGGAGTCAA GATGATTGTA GTTCTTGGGG AGATAGGGGG TACAGAAGAA TATAAGATCT 
     3181 GCCGGGGCAT CAAGGAGGGC CGCCTCACCA AGCCAGTGGT CTGCTGGTGC ATCGGGACCT 
     3241 GTGCCACCAT GTTCTCTTCT GAGGTCCAGT TTGGCCACGC TGGGGCTTGT GCCAACCAGG 
     3301 CTTCTGAAAC GGCAGTAGCC AAGAACCAGG CCTTGAAGGA AGCGGGAGTG TTTGTGCCCC 
     3361 GAAGCTTTGA TGAGCTCGGA GAAATCATTC AGTCCGTGTA TGAAGATCTT GTGGCCAAAG 
     3421 GCGCCATTGT ACCTGCTCAG GAAGTGCCAC CTCCAACAGT ACCCATGGAC TACTCTTGGG 
     3481 CCAGGGAGCT GGGTTTAATC CGAAAACCTG CCTCATTCAT GACCAGCATC TGTGACGAGC 
     3541 GGGGGCAGGA ACTCATTTAT GCGGGCATGC CCATCACCGA GGTCTTCAAG GAAGAGATGG 
     3601 GCATTGGTGG TGTCCTGGGC CTCCTCTGGT TCCAGAGAAG GTTGCCCAAG TATTCCTGCC 
     3661 AGTTCATTGA GATGTGTCTC ATGGTCACCG CTGATCACGG GCCAGCTGTC TCCGGGGCCC 
     3721 ATAACACTAT CATCTGTGCT CGGGCTGGGA AGGACCTGGT CTCCAGCCTC ACCTCAGGGC 
     3781 TGCTCACCAT TGGGGACCGG TTTGGGGGTG CCTTGGACGC AGCAGCGAAG ATGTTCAGTA 
     3841 AAGCCTTTGA CAGCGGCATT ATTCCCATGG AGTTTGTGAA CAAGATGAAG AAGGAGGGGA 
     3901 AACTGATCAT GGGCATCGGC CATCGAGTCA AATCGATAAA CAACCCAGAC ATGCGAGTGC 
     3961 AGATCCTCAA AGACTTTGTC AAACAGCACT TCCCCGCCAC CCCGCTGCTC GACTATGCAC 
     4021 TGGAAGTGGA GAAAATCACC ACCTCAAAGA AGCCAAATCT TATCCTGAAC GTGGATGGTT 
     4081 TCATCGGCGT TGCGTTTGTG GACATGCTTA GGAACTGTGG CTCCTTCACC CGGGAGGAAG 
     4141 CTGACGAGTA TGTTGACATT GGAGCCCTCA ATGGCGTCTT TGTGCTGGGA AGGAGTATGG 
     4201 GCTTCATCGG GCACTATCTT GACCAGAAGA GGCTGAAGCA AGGGCTGTAT CGTCACCCCT 
     4261 GGGACGACAT TTCCTATGTT CTCCCGGAAC ACATGAGCAT GACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//