LOCUS       RR200497                6897 bp    DNA     circular  SYN 
DEFINITION  Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug)
ACCESSION   NM_017032
VERSION     NM_017032.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3054..3145 
                     /label="Myc-DDK"  
     misc_feature    3175..3760 
                     /label="PolyA signal"  
     misc_feature    3232..3251 
                     /label="Restriction Enzyme"  
     misc_feature    4036..4708 
                     /label="ColE1"  
     misc_feature    5138..5932 
                     /label="Kan/Neo"  
     misc_feature    5967..6269 
                     /label="SV40 ori"  
     misc_feature    6355..6832 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3036..3042 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3036 
                     /label="Pde4d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCATGTG AATACCTTCC CTTTCAGAAG GCATTCCTGG ATATGTTTCG 
     1081 ATGTGGACAA TGGCACATCA GCGGGACGGA GTCCCTTGGA TCCCATGACC AGCCCAGGGT 
     1141 CTGGGCTAAT TCTCCAAGCA AACTTTGTGC ACAGTCAACG CCGGGAGTCC TTCCTGTATC 
     1201 GATCAGACAG CGATTATGAC CTCTCTCCAA AGTCTATGTC CAGGAACTCC TCGATTGCCA 
     1261 GTGATATACA TGGAGATGAC TTGATTGTGA CTCCATTTGC TCAGGTCTTG GCCAGCCTTC 
     1321 GAACTGTAAG AAACAACTTT GCTGCATTAA CTAATTTGCA AGATCGCGCA CCCAGCAAAA 
     1381 GATCACCCAT GTGCAACCAA CCATCCATCA ACAAAGCCAC CATCACAGAG GAGGCCTACC 
     1441 AGAAACTGGC CAGCGAGACC CTGGAGGAGC TGGACTGGTG TCTGGACCAG CTAGAGACCC 
     1501 TGCAGACCAG GCACTCCGTC AGTGAGATGG CCTCCAACAA GTTCAAGAGG ATGCTTAATC 
     1561 GGGAGCTCAC CCACCTCTCT GAAATGAGTC GGTCTGGCAA CCAGGTGTCG GAGTACATAT 
     1621 CAAACACGTT CTTAGATAAG CAACATGAAG TAGAAATTCC CTCTCCGACT CAGAAGGAAA 
     1681 AAGAGAAGAA GAAAAGGCCG ATGTCACAGA TCAGTGGGGT CAAGAAGTTG ATGCACAGCT 
     1741 CCAGCCTGAC CAATTCCTGC ATTCCAAGAT TTGGGGTTAA AACAGAGCAG GAAGATGTCC 
     1801 TGGCCAAGGA ACTAGAAGAC GTGAACAAGT GGGGCCTCCA CGTTTTCCGA ATAGCGGAGC 
     1861 TGTCTGGCAA CCGGCCTCTG ACTGTTATCA TGCACACCAT TTTTCAGGAA CGAGATTTGT 
     1921 TAAAAACGTT TAAAATCCCA GTGGACACTT TGATTACGTA TCTTATGACT CTAGAAGACC 
     1981 ATTACCATGC TGACGTGGCC TATCACAACA ACATCCATGC TGCAGATGTC GTCCAGTCAA 
     2041 CTCATGTGCT GCTCTCTACA CCCGCTTTGG AGGCTGTTTT CACTGACTTG GAGATTCTCG 
     2101 CGGCCATTTT TGCCAGTGCA ATACATGATG TGGATCATCC TGGTGTGTCA AATCAATTTC 
     2161 TGATCAATAC AAACTCGGAA CTTGCCTTGA TGTACAACGA CTCCTCCGTC TTAGAGAATC 
     2221 ATCATTTGGC TGTGGGCTTT AAGTTGCTCC AGGAAGAAAA CTGTGACATT TTCCAGAATC 
     2281 TGACCAAAAA GCAAAGACAA TCTTTAAGGA AAATGGCCAT TGACATTGTA CTAGCGACAG 
     2341 ACATGTCAAA GCACATGAAT CTGCTGGCTG ATCTGAAAAC AATGGTTGAA ACGAAGAAGG 
     2401 TGACGAGCTC TGGCGTCCTC CTCCTTGATA ACTATTCTGA CAGGATCCAG GTCCTCCAGA 
     2461 ATATGGTGCA CTGTGCAGAC CTGAGCAACC CCACAAAGCC ACTCCAGCTC TACCGCCAGT 
     2521 GGACGGACCG GATAATGGAG GAGTTCTTCC GTCAGGGGGA CCGGGAGCGT GAGCGTGGCA 
     2581 TGGAGATAAG TCCCATGTGT GACAAGCACA ACGCCTCTGT GGAAAAATCA CAGGTGGGCT 
     2641 TCATAGACTA CATTGTTCAT CCACTCTGGG AGACATGGGC AGACCTCGTA CATCCCGACG 
     2701 CCCAGGACAT TTTGGACACT TTGGAGGACA ATCGTGAGTG GTACCAGAGC ACAATCCCCC 
     2761 AGAGCCCCTC CCCTGCACCT GATGACCAAG AGGACGGCCG TCAGGGACAG ACTGAAAAAT 
     2821 TCCAGTTCGA ACTAACCTTA GAGGAAGATG GCGAGTCAGA CACTGAAAAG GACAGTGGAA 
     2881 GTCAAGTGGA GGAAGACACT AGCTGCAGTG ACTCTAAGAC TCTGTGCACC CAAGACTCAG 
     2941 AGTCCACTGA AATTCCCCTT GACGAGCAGG TTGAAGAGGA GGCTGTAGCA GAAGAGGAAA 
     3001 GCCAGCCCCA AACTGGCGTC GCAGACGATT GTTGTCCTGA CACGACGCGT ACGCGGCCGC 
     3061 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3121 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3181 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3241 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3301 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3361 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3421 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3481 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3541 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3601 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3661 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3721 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3781 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3841 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     3901 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     3961 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4021 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4081 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4141 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4201 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4261 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4321 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4381 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4441 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4501 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4561 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4621 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4681 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4741 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4801 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4861 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     4921 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     4981 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5041 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5101 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5161 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5221 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5281 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5341 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5401 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5461 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5521 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5581 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5641 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5701 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5761 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5821 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     5881 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     5941 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6001 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6061 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6121 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6181 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6241 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6301 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6361 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6421 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6481 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6541 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6601 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6661 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6721 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6781 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6841 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//