LOCUS       RR200483                8235 bp    DNA     circular  SYN 
DEFINITION  Ret (Myc-DDK-tagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 1, (10 ug)
ACCESSION   NM_012643
VERSION     NM_012643.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4390..4481 
                     /label="Myc-DDK"  
     misc_feature    4511..5096 
                     /label="PolyA signal"  
     misc_feature    4568..4587 
                     /label="Restriction Enzyme"  
     misc_feature    5372..6044 
                     /label="ColE1"  
     misc_feature    6474..7268 
                     /label="Kan/Neo"  
     misc_feature    7303..7605 
                     /label="SV40 ori"  
     misc_feature    7691..8168 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4365..4372 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4365 
                     /label="Ret"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAAAGCG AGGTCCGGCG CCGCAGGGCT GGGGCTGAAG CTGTTTTTGC 
     1081 TGCTGCCGCT ACTGGGAGAA GCCCCGCTGG GTCTCTACTT CTCAAGGGAT GCTTACTGGG 
     1141 AGAGGCTGTA TGTGGACCAG CCAGCTGGCA CACCTCTGCT CTATGTCCAT GCCCTACGGG 
     1201 ATGCCCCTGG AGAAGTGCCC AGCTTCCGCC TGGGCCAGTA TCTCTATGGC GTCTACCGCA 
     1261 CGCGTCTGCA TGAGAATGAC TGGATCCACA TCGATTCGGG CACTGGCCTC CTCTACCTCA 
     1321 ATCAGAGCCT GGACCATAGT TCCTGGGAGC AGCTCAGCAT CCGCAATGGC GGCTTCCCCT 
     1381 TGCTCACCGT CTTCCTCCAG GTCTTCCTGG GGTCCACAGC CCAGAGAGAG GGAGAGTGTC 
     1441 ATTGGCCAGG CTGTGCCCGT GTGTACTTCT CCTTCATCAA CGACACCTTC CCAAATTGTA 
     1501 GCTCCTTCAA AGCCCGGGAT CTCTGCACCC CGGAGACGGG TGTGTCCTTC CGCATCAGGG 
     1561 AGAACAGGCC CCCTGGCACC TTCTACCAGT TCCGCATGCT ACCTGTGCAG TTCCTTTGTC 
     1621 CTAACATCAG TGTGAAGTAC AAACTCTTAG AAGGGGACGG TCTGCCCTTC CGTTGTGACC 
     1681 CCGACTGTCT GGAGGTGAGC ACGCGGTGGG CGCTGGATCG GGAGCTTCAG GAGAAGTATG 
     1741 TGCTGGAGGC TGAGTGCGCA GTGGCAGGCC CTGGAGCCAA CAAGGAGAAG GTGGCCGTGT 
     1801 CCTTCCCGGT GACGGTGTAT GATGAAGACG ACTCCCCGCC CACCTTCTCC GGAGGTGTGG 
     1861 GCACCGCCAG TGCTGTGGTG GAGTTTAAGC GGAAGGAGGG CACTGTGGTA GCCACTCTGC 
     1921 AGGTGTTTGA TGCAGATGTG GTGCCAGCAT CTGGGGAGCT GGTGAGGCGG TACACAAGCA 
     1981 CACTACTCTC AGGGGATTCC TGGGCCCAGC AGACCTTCCG GGTGGAGCAC ACACCCAACG 
     2041 AGACCTTGGT CCAGTCCAAC AACAACTCCG TGCGGGCAAC CATGCACAAT TACAAGCTGG 
     2101 TTCTCAACAG GAGCCTGTCC ATCTCAGAGA GCCGAGTCCT GCAGCTAGTA GTCCTGGTCA 
     2161 ATGACTCAGA CTTCCAGGGG CCTGGGTCAG GTGTTCTCTT CCTCCATTTC AACGTGTCTG 
     2221 TGCTGCCTGT CACCCTGAAC CTACCCATGG CCTACTCCTT CCCAGTGAAT AGGAGAGCCC 
     2281 GCCGTTATGC CCAGATTGGG AAAGTTTGCG TGGAGAACTG CCAGGAGTTC AGCGGTGTCT 
     2341 CCATCCAGTA CAAGCTGCAG CCCTCCAGCA CCAACTGCAG TGCCCTAGGT GTGGTCACCT 
     2401 CAACAGAAGA CACCTCAGGG ACCCTATATG TAAATGACAC GGAGGCCCTG CGGCGACCTG 
     2461 AGTGTACCGA GCTTCAGTAC ACAGTGGTAG CCACTGACCG GCAGACCCGC AGGCAGACCC 
     2521 AGGCTTCGTT AGTCGTCACA GTGGAGGGGA CATACATTGC AGAAGAAGTG GGCTGCCCCA 
     2581 AGTCCTGTGC AGTAAACAAG AGGCGACCTG AGTGTGAGGA GTGTGGTGGC CTGGGTTCTC 
     2641 CAACTGGCAG ATGTGAGTGG CGTCAGGGAG ATGGTAAAGG GATCACCAGG AACTTCTCCA 
     2701 CCTGTTCTCC TAGCACCAGG ACCTGTCCTG ATGGCCACTG TGATGCTCTG GAGAGCCGGG 
     2761 ATATCAACAT TTGCCCCCAG GACTGTCTCC GTGGCCCCAT TGTTGGCGGG CATGAGCGAG 
     2821 GGGAGCGCCA GGGGATTAAA GCCGGCTATG GCATCTGCAA CTGTTTCCCT GATGAGAAGA 
     2881 AGTGCTTCTG CGAGCCAGAG GACAGCCAGG GCCCATTGTG CGATGAGCTG TGCCGTACGG 
     2941 TCATCACAGC CGCTGTCCTC TTCTCCTTCA TAATCTCTGT CCTGCTGTCC ACCTTCTGCA 
     3001 TCCACCGCTA CCACAAGCAT GCGCACAAGC CACCCATCGC GTCAGCCGAA ATGACCTTCT 
     3061 GCCGGCCGGC CCAGGGCTTC CCAATCAGCT ATTCTTCCTC GGGCACCCGC CGGCCCTCAC 
     3121 TGGACTCCAT GGAGAACCAG GTCTCTGTGG ACTCTTTCAA GATCCCGGAG GATCCGAAGT 
     3181 GGGAATTTCC TCGGAAGAAC TTAGTTCTTG GGAAAACCCT GGGAGAAGGC GAGTTTGGAA 
     3241 AAGTAGTCAA GGCCACAGCC TTCCGTCTGA AAGGCCGGGC AGGATACACC ACAGTGGCTG 
     3301 TGAAAATGCT GAAAGAAAAC GCCTCCCAGA GTGAACTACG AGACCTGCTC TCTGAGTTCA 
     3361 ACCTTCTGAA ACAAGTCAAC CATCCACATG TCATCAAGTT GTACGGGGCT TGCAGCCAGG 
     3421 ATGGGCCACT TCTTCTCATT GTGGAGTATG CAAAGTATGG ATCCCTGCGG GGGTTCCTGC 
     3481 GGGACAGCCG CAAGATCGGG CCTGCCTATG TGAGCAGTGG AGGCAGCCGC AATTCCAGCT 
     3541 CCCTGGACCA CCCAGACGAA AGGGTGCTGA CCATGGGCGA CCTCATCTCC TTCGCCTGGC 
     3601 AGATCTCGAG GGGTATGCAG TACTTGGCTG AAATGAAGCT CGTACATCGA GACTTAGCTG 
     3661 CCAGAAACAT CTTGGTGGCA GAGGGACGGA AGATGAAGAT CTCTGACTTT GGGCTGTCCC 
     3721 GAGATGTTTA TGAAGAAGAT TCCTATGTGA AGAAAAGCAA GGGCCGGATT CCCGTCAAAT 
     3781 GGATGGCAAT CGAGTCTCTC TTCGATCACA TCTATACCAC TCAAAGTGAT GTGTGGTCCT 
     3841 TTGGAGTGCT GCTATGGGAG ATTGTGACCC TGGGAGGCAA CCCCTACCCT GGAATTCCTC 
     3901 CTGAACGACT CTTCAACCTT CTGAAGACAG GCCACAGGAT GGAGAGGCCA GACAACTGCA 
     3961 GCGAGGAAAT GTACCGCCTG ATGCTGCAGT GCTGGAAGCA GGAGCCGGAC AAGAGGCCAG 
     4021 TATTTGCTGA CATCAGCAAG GATCTGGAGA AGATGATGGT CAAAAGCAGA GACTACTTGG 
     4081 ACCTGGCTGC ATCCACCCCT TCGGACTCAC TGCTCTATGA CGATGGGCTC TCGGAAGAGG 
     4141 AGACGCCCCT GGTGGACTGT AACAGTGCTC CCCTCCCGCG CTCCCTCCCT TCCACATGGA 
     4201 TTGAAAACAA ACTCTATGGC ATGTCAGACC CGAACTGGCC TGGAGAGAGT CCTGTACCAC 
     4261 TCACGAGAGC CGATGGCACT AGCACTGGGT TCCCAAGATA TGCAAATGAT AGTGTATATG 
     4321 CTAACTGGAT GGTTTCACCC TCAGCGGCAA AATTAATGGA CACATTTGAT AGTAGCGGAC 
     4381 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//