LOCUS       RR200466                8307 bp    DNA     circular  SYN 
DEFINITION  Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug)
ACCESSION   NM_021845
VERSION     NM_021845.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4462..4553 
                     /label="Myc-DDK"  
     misc_feature    4583..5168 
                     /label="PolyA signal"  
     misc_feature    4640..4659 
                     /label="Restriction Enzyme"  
     misc_feature    5444..6116 
                     /label="ColE1"  
     misc_feature    6546..7340 
                     /label="Kan/Neo"  
     misc_feature    7375..7677 
                     /label="SV40 ori"  
     misc_feature    7763..8240 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4460 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="Gnas"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCATGCTC AACTGCCTCC ACGGCAATAA TATGTCAGGA CAACACGATA 
     1081 TCCCCCCTGA AGTCGGGGAC CAGCCCGAGC AAGAACCTTT GGAAGCCCAA GGGGCCGCTG 
     1141 CCCCCGGTGC TGGGGTTGGC CCAGCCGAAG AAATGGAGAC CGAACCGTCT AACAATGAGC 
     1201 CCATCCCCGA CGAGACTGAC AGTGAGGTCT GTGGACCCCC TGAAGACTCC AAATCTGACA 
     1261 TCCAGAGCCC CAGTCAGGCC TTCGAGGAAG TCCAAGTGGG CGGAGACTAC AGCCCACCTC 
     1321 CAGAGGAAGC CATGCCATTC GAGATCCAAC AGCCCAGCCT GGGAGATTTC TGGCCCACCC 
     1381 TGGAGCAGCC TGGACCATCT GGGACCCCAT CAGGCATCAA AGCCTTCAAC CCAGCGATTT 
     1441 TGGAGCCCGG GACCCCCACT GGTGCACACC CAGGCCTGGG AGCCTATAGC CCCCCACCAG 
     1501 AAGAAGCTAT GCCATTTGAG TTCAATGAGC CTGCCCAGGA AGACCGTTGC CAGCCTCCCT 
     1561 TGCAAGTCCC AGACCTTGCG CCAGGGGGTC CGGAAGCATG GGTCTCCAGA GCTCTTCCCG 
     1621 CGGAGCCCGG GAACCTCGGA TTTGAAAACA CTGGCTTCCG AGAAGACTAC AGCCCTCCAC 
     1681 CTGAAGAATC TGTGCCATTT CAGCTCGATG GAGAAGAATT CGGGGGCGAT AGCCCACCCC 
     1741 CAGGACTCCC GCGAGTCACG CCACAAATCG GCATTGGCGG CGAGTTCCCG ACAGTCGCGG 
     1801 TCCCGAGTAC GCTCTGCCTC GCTCCCGCCG CGAACGCGCC TCCCCTCTGG GTCCAGGGCG 
     1861 CCATTGGCAG ACCATTCCGC GAGGCTGTCA GATCTCCTAA CTTCGCATAC GACATTTCCC 
     1921 CAATGGAGAT CACCAGACCC CTGCTTGAGA TTGGCAGAGC CTCCACTGGG GTCGACGACG 
     1981 ACACCGCTGT CAATATGGAC AGCCCCCCAA TCGCAAGTGA TGGCCCGCCC ATCGAAGTCT 
     2041 CGGGAGCCCC AGTTAAGAGC GAGCACGCAA AGAGACCCCC ACTTGAGCGA CAAGCAGCCG 
     2101 AGACAGGAAA CAGCCCTATC AGCAGCACCA CTGCAGAGGA GGCAAAAGTC CCCTCCCTCG 
     2161 AGCGAGGAGA AGGATCCCCC ACCCAACCTG AAACAGTGCA TATCAAGCCA GCTCCTGTTG 
     2221 CGGAGTCCGG AACGGACTCT TCCAAAGCCG ATCCGGACTC AGCTACACAC GCAGTTCTTC 
     2281 AGATCGGTCC TGAGGAAGTC GGAGGAGTCC CAACCATGCC CACCGATCTT CCGCCTGCTT 
     2341 CTGAAGATGC GGGCCCAGAT GTCCGGGCAG AACCAGACGG AGGGACAGCC CCAGCCACCC 
     2401 CTGCCGAGTC CGAAGACAAC AGAGAACCAG CCGCCGCCGC CGCCGCCGAG CCAGCCGCCG 
     2461 AGCCAGCCGC TGAGCCAGCC GCCGAGCCAG CCGCCGAGCC AGCCGCCGAG CCAGCTGCCG 
     2521 AGGCAGTCCC TGACACCGAA GCCGAGTCTG CCTCCGGGGC AGTCCCCGAC ACCCAAGAGG 
     2581 AGCCCGCAGC CGCGGCAGCC TCTGCCACGC CGGCGGAGCC TGCCGCCCGG GCAGCCCCCG 
     2641 TCACCCCTAC GGAGCCCGCT ACCCGGGCTG TCCCTTCTGC CAGAGCCCAT CCAGCCGCCG 
     2701 GGGCTGTCCC TGGAGCCTCA GCGATGTCAG CCGCTGCTAG GGCAGCCGCC GCTAGAGCAG 
     2761 CCTATGCAGG TCCTCTGGTC TGGGGAGCCA GGTCACTCTC GGCTACTCCA GCCGCTCGGG 
     2821 CATCCCTCCC TGCCCGCGCA GCAGCTGCCG CCCGAGCAGC CTCTGCTGCC CGCGCAGTCG 
     2881 CTGCCGGCCG GTCAGCCTCT GCCGCCCCAA GCAGGGCCCA TCTTAGACCC CCCAGCCCGG 
     2941 AGATCCAGGT TGCTGACCCG CCTACTCCGC GGCCTGCTCC GCGGCCGAGT GCCTGGCCTG 
     3001 ACAAATACGA ACGTGGCCGA AGCTGCTGCA GGTATGAGGC TGCGTCCGGC ATCTGCGAGA 
     3061 TCGAGTCCTC CAGCGATGAG TCGGAAGAAG GGGCCACTGG CTGCTTCCAG TGGCTTCTGC 
     3121 GGCGAAACCG CCGCCCTGGC CAGCCCCGGA GCCACACAGT CGGGAGCAAC CCGGTCCGCA 
     3181 ACTTCTTCGC CCGAGCCTTC GGAAGCTGCT TCGGTCTATC TGAGTGTACC CGATCACGAT 
     3241 CCCTCAGCCC CGGGAAGGCC AAGGATCCTA TGGAAGAGAG GCGCAAACAG ATGCGCAAAG 
     3301 AAGCCATGGA GATGCGAGAG CAGAAGCGCG CAGATAAGAA ACGCAGCAAG CTCATCGACA 
     3361 AGCAACTGGA GGAGGAGAAG ATGGACTACA TGTGTACACA CCGCCTGCTG CTTCTAGGTG 
     3421 CTGGAGAGTC TGGCAAAAGC ACCATTGTGA AGCAGATGAG GATCCTACAT GTTAATGGGT 
     3481 TTAACGGAGA GGGCGGCGAA GAGGACCCGC AGGCTGCAAG GAGCAACAGC GATGGTGAGA 
     3541 AGGCCACCAA AGTGCAGGAC ATCAAAAACA ACCTGAAGGA GGCCATTGAA ACCATTGTGG 
     3601 CCGCCATGAG CAACCTGGTG CCCCCCGTGG AGCTGGCCAA CCCTGAGAAC CAGTTCAGAG 
     3661 TGGACTACAT TCTGAGCGTG ATGAACGTGC CAAACTTTGA CTTCCCACCT GAATTCTATG 
     3721 AGCATGCCAA GGCTCTGTGG GAGGATGAGG GAGTTCGTGC CTGCTACGAG CGCTCCAACG 
     3781 AGTACCAGCT GATCGACTGT GCCCAGTACT TCCTGGACAA GATTGATGTG ATCAAGCAGG 
     3841 CCGACTACGT GCCAAGTGAC CAGGACCTGC TTCGCTGCCG CGTCCTGACC TCTGGAATCT 
     3901 TTGAGACCAA GTTCCAGGTG GACAAAGTCA ACTTCCACAT GTTCGATGTG GGCGGCCAGC 
     3961 GCGATGAACG CCGCAAGTGG ATCCAGTGCT TCAATGATGT GACTGCCATC ATCTTCGTGG 
     4021 TGGCCAGCAG CAGCTACAAC ATGGTCATCC GGGAGGACAA CCAGACCAAC CGTCTGCAGG 
     4081 AGGCTCTGAA CCTCTTCAAG AGCATCTGGA ACAACAGATG GCTGCGTACC ATCTCTGTGA 
     4141 TCCTCTTCCT CAACAAGCAA GATCTGCTTG CTGAGAAGGT CCTCGCTGGG AAATCGAAGA 
     4201 TTGAGGACTA CTTTCCAGAG TTCGCTCGCT ACACCACTCC TGAGGATGCG ACTCCCGAGC 
     4261 CCGGAGAGGA CCCACGCGTG ACCCGGGCCA AGTACTTCAT CCGGGATGAG TTTCTGAGAA 
     4321 TCAGCACTGC TAGTGGAGAT GGACGTCACT ACTGCTACCC TCACTTTACC TGCGCCGTGG 
     4381 ACACTGAGAA CATCCGCCGT GTCTTCAACG ACTGCCGTGA CATCATCCAG CGCATGCATC 
     4441 TTCGCCAATA CGAGCTGCTC ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4501 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4561 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4621 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4681 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4741 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4801 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4861 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4921 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4981 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5041 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5101 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5161 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5221 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5281 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5341 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5401 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5461 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5521 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5581 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5641 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5701 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5761 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5821 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5881 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5941 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6001 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6061 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6121 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6181 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6241 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6301 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6361 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6421 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6481 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6541 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6601 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6661 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6721 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6781 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6841 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6901 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6961 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7021 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7081 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7141 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7201 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7261 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7321 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7381 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7441 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7501 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7741 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7801 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7861 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7921 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7981 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8041 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8101 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8161 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8221 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8281 ATTTAACAAA AATTTAACGC GAATTTT
//