LOCUS       RR200423                6816 bp    DNA     circular  SYN 
DEFINITION  Bcan (Myc-DDK-tagged ORF) - Rat brevican (Bcan), transcript variant 2, (10 ug)
ACCESSION   NM_012916
VERSION     NM_012916.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2973..3064 
                     /label="Myc-DDK"  
     misc_feature    3094..3679 
                     /label="PolyA signal"  
     misc_feature    3151..3170 
                     /label="Restriction Enzyme"  
     misc_feature    3955..4627 
                     /label="ColE1"  
     misc_feature    5057..5851 
                     /label="Kan/Neo"  
     misc_feature    5886..6188 
                     /label="SV40 ori"  
     misc_feature    6274..6751 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2955..2961 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2955 
                     /label="Bcan"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCCATTG CTTCTGTCCC TGCTGGCAGC TCTGGTCCTG ACCCAAGCCC 
     1081 CTGCAGCCCT CGCTGATGAC CTGAAAGAAG ACAGCTCAGA GGATCGAGCC TTTCGGGTGC 
     1141 GCATCGGTGC CGCGCAGCTG CGGGGTGTGC TGGGCGGTGC CCTGGCCATC CCATGCCACG 
     1201 TCCACCACCT GAGGCCGCCG CCCAGCCGCC GGGCCGCGCC GGGCTTTCCC CGAGTCAAAT 
     1261 GGACCTTCCT GTCCGGGGAC CGGGAGGTGG AGGTGCTGGT GGCGCGCGGG CTGCGCGTCA 
     1321 AGGTAAACGA AGCCTATCGG TTCCGCGTGG CGCTGCCTGC CTACCCCGCA TCGCTCACAG 
     1381 ATGTGTCTTT AGTATTGAGC GAACTGCGGC CCAATGATTC CGGGGTCTAT CGCTGCGAGG 
     1441 TCCAGCACGG TATCGACGAC AGCAGTGATG CTGTGGAAGT CAAGGTCAAA GGGGTCGTCT 
     1501 TCCTCTACCG AGAGGGCTCT GCCCGCTATG CTTTCTCCTT CGCTGGAGCC CAGGAAGCCT 
     1561 GTGCTCGCAT CGGAGCCCGA ATTGCCACCC CTGAGCAGCT GTATGCTGCC TACCTCGGCG 
     1621 GCTATGAACA GTGTGATGCT GGCTGGCTGT CCGACCAAAC CGTGAGGTAC CCCATCCAGA 
     1681 ACCCACGAGA AGCCTGTTAT GGAGACATGG ATGGCTACCC TGGAGTGCGG AATTACGGAG 
     1741 TGGTGGGTCC TGATGATCTC TACGATGTCT ACTGTTATGC CGAAGACCTA AATGGAGAAC 
     1801 TGTTCCTAGG TGCCCCTCCC GGCAAGCTGA CGTGGGAGGA GGCTCGGGAC TACTGTCTGG 
     1861 AACGCGGTGC TCAGATCGCT AGCACGGGCC AGCTATACGC GGCATGGAAT GGCGGCTTGG 
     1921 ACAGATGTAG CCCTGGCTGG CTGGCTGATG GCAGTGTGCG GTACCCCATC ATCACGCCCA 
     1981 GCCAACGCTG TGGGGGAGGC CTGCCAGGAG TCAAGACCCT CTTCCTCTTT CCCAACCAGA 
     2041 CTGGCTTCCC CAGCAAGCAG AACCGCTTCA ATGTCTACTG CTTCCGAGAC TCTGCCCATC 
     2101 CCTCTGCCTT CTCTGAGGCC TCCAGCCCAG CCTCTGATGG ACTAGAGGCC ATTGTCACAG 
     2161 TGACAGAGAA GCTGGAGGAA CTGCAGTTGC CTCAGGAAGC TGTGGAGAGC GAGTCTCGTG 
     2221 GGGCGATCTA CTCCATCCCC ATCACAGAAG ATGGGGGAGG AGGAAGCTCT ACCCCAGAAG 
     2281 ACCCAGCAGA GGCCCCCAGG ACTCCTCTAG AATCAGAAAC CCAATCCGTT GCACCACCTA 
     2341 CCGGGTCCTC AGAAGAGGAA GGCGAAGCCC TGGAGGAAGA AGAAAGATTC AAAGACACAG 
     2401 AGACTCCGAA GGAAGAGAAG GAGCAGGAGA ACCTGTGGGT GTGGCCCACG GAGCTCAGCA 
     2461 GCCCTCTCCC TACTGGCTTG GAAACAGAGC ACTCACTCTC CCAGGTGTCC CCACCAGCCC 
     2521 AGGCAGTTCT ACAGCTGGGT GCATCACCTT CTCCCAGGCC TCCAAGGGTC CATGGACCGC 
     2581 CTGCAGAGAC TTTGCAACCC CCAAGGGAGG GAAGCCTCAC ATCTACTCCA GATGGGGCAA 
     2641 GAGAAGTAGC GGGGGAAACT GGGAGCCCTG AGCTCTCTGG GGTTCCTCGA GAAAGCGAGG 
     2701 AGGCAGGAAG CTCCAGCTTG GAGGATGGCC CTTCCCTCCT TCCAGCGACA TGGGCCCCTG 
     2761 TGGGTACCAG GGAGCTGGAG ACCCCCTCAG AAGAGAAGTC TGGAAGAACT GTTCTGACAG 
     2821 GCACATCAGT GCAGGCCCAG CCAGTGCTGC CCACCGACAG TGCCAGCCGA GGTGGAGTGG 
     2881 CTGTGGCTCC CTCATCAGGT AATTCTGCCG AAGGCTCGAT GCCCGCCTTT CTCCTTTTCC 
     2941 TCCTCCTCCA GCTCTGGGCC ACCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3001 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3061 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3121 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3181 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3241 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3301 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3361 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3421 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3481 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3541 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3601 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3661 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3721 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3781 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3841 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3901 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3961 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4021 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4081 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4141 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4201 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4261 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4321 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4381 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4441 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4501 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4561 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4621 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4681 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4741 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4801 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4861 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4921 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4981 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5041 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5101 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5161 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5221 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5281 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5341 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5401 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5461 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5521 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5581 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5641 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5701 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5761 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5821 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5881 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5941 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6001 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6061 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6121 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6181 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6241 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6301 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6361 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6421 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6481 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6541 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6601 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6661 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6721 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6781 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//