LOCUS       RR200421                8133 bp    DNA     circular  SYN 
DEFINITION  Cacna2d1 (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant 2, (10 ug)
ACCESSION   NM_001110848
VERSION     NM_001110848.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4290..4381 
                     /label="Myc-DDK"  
     misc_feature    4411..4996 
                     /label="PolyA signal"  
     misc_feature    4468..4487 
                     /label="Restriction Enzyme"  
     misc_feature    5272..5944 
                     /label="ColE1"  
     misc_feature    6374..7168 
                     /label="Kan/Neo"  
     misc_feature    7203..7505 
                     /label="SV40 ori"  
     misc_feature    7591..8068 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4272..4278 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4272 
                     /label="Cacna2d1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTGGC TGCCTGCTGG CCTTGACTCT GACACTTTTC CAATCTTGGC 
     1081 TGATCGGCCC CTCGAGCGAG GAGCCCTTCC CTTCGCCCGT CACTATCAAG TCATGGGTGG 
     1141 ATAAAATGCA GGAAGACCTT GTCACACTGG CAAAGACAGC AAGTGGAGTG ACTCAGCTTG 
     1201 CTGACATTTA TGAAAAATAC CAAGATTTGT ATACTGTGGA GCCAAATAAT GCACGCCAAC 
     1261 TGGTTGAAAT TGCAGCTAGA GACATTGAGA AACTTCTGAG CAACAGATCT AAAGCCCTGG 
     1321 TGCGCCTGGC TATGGAAGCA GAGAAAGTCC AAGCCGCCCA CCAATGGAGG GAAGATTTTG 
     1381 CAAGCAACGA AGTTGTCTAC TACAACGCTA AAGACGATCT TGATCCTGAA AGAAATGAGA 
     1441 GTGAGTCAGG CAGCCAACGG ATTAAACCTG TTTTCATCGA AGACGCTAAT TTTGGACGCC 
     1501 AGATATCCTA TCAACATGCA GCGGTCCATA TTCCCACGGA CATCTATGAG GGCTCAACCA 
     1561 TAGTGTTAAA CGAACTCAAC TGGACAAGTG CCTTAGATGA AGTTTTCAAA AGAAATCGAG 
     1621 ATGAAGACCC TACATTGCTG TGGCAAGTGT TTGGCAGCGC CACCGGCCTG GCCCGATATT 
     1681 ATCCAGCTTC TCCGTGGGTG GATAACAGCA GAACTCCAAA CAAGATCGAT CTCTACGATG 
     1741 TACGCAGAAG ACCATGGTAC ATCCAGGGAG CTGCATCCCC CAAAGACATG CTCATTCTGG 
     1801 TGGACGTGAG TGGAAGTGTG AGCGGATTGA CTCTGAAACT CATCCGGACA TCTGTCTCTG 
     1861 AGATGTTAGA AACCCTTTCC GATGATGATT TCGTGAATGT AGCTTCATTT AACAGCAATG 
     1921 CTCAGGATGT GAGTTGTTTC CAGCACCTGG TTCAAGCGAA TGTAAGGAAC AAGAAGGTGC 
     1981 TGAAGGATGC GGTGAATAAC ATCACAGCAA AGGGGATAAC AGATTACAAG AAAGGCTTTA 
     2041 GCTTCGCGTT TGAACAGCTA CTTAATTATA ATGTTTCCAG AGCCAATTGT AATAAGATCA 
     2101 TCATGTTATT CACGGATGGA GGAGAAGAGA GAGCCCAGGA GATATTTGCC AAGTACAATA 
     2161 AAGACAAAAA AGTCCGCGTG TTTACATTTT CTGTTGGTCA ACATAATTAT GACAGAGGAC 
     2221 CTATTCAGTG GATGGCTTGT GAAAACAAAG GTTATTATTA TGAGATTCCC TCTATTGGTG 
     2281 CAATAAGAAT CAATACTCAG GAATATCTAG ATGTTCTGGG AAGACCAATG GTTTTAGCCG 
     2341 GGGACAAAGC AAAGCAAGTC CAGTGGACAA ATGTGTATTT GGATGCTCTG GAGCTGGGAC 
     2401 TTGTCATTAC TGGAACTCTA CCGGTCTTCA ACGTCACTGG CCAGTCTGAA AATAAGACTA 
     2461 ACTTGAAGAA CCAGTTGATT CTTGGTGTGA TGGGCGTTGA TGTGTCTCTG GAAGATATTA 
     2521 AGAGATTGAC ACCACGTTTT ACACTGTGCC CCAACGGTTA CTATTTTGCA ATCGATCCTA 
     2581 ATGGTTATGT CTTACTGCAT CCAAATCTTC AGCCAAAGAA CCCCAAATCT CAGGAGCCGG 
     2641 TCACACTGGA TTTTCTCGAT GCTGAGTTAG AGAATGATAT TAAAGTGGAG ATTCGAAATA 
     2701 AAATGATAGA CGGAGAAAGT GGAGAAAAAA CATTCAGAAC TCTGGTCAAA TCTCAAGATG 
     2761 AGAGATTTAT TGACAAAGGA AATCGAACAT ACACATGGAC ACCTGTCAAT GGCACAGATT 
     2821 ACAGTTTGGC CTTGGTATTG CCAACCTACA GTTTTTACTA TATAAAAGCC AAAATAGAAG 
     2881 AGACAATAAC TCAGGCCAGA TATTCAGAAA CCCTGAAGCC AGACAATTTT GAAGAATCTG 
     2941 GCTACACTTT CATAGCACCA AGGGAATACT GCAATGACCT GAAACCTTCA GATAATAACA 
     3001 CTGAATTTCT TTTAAATTTC AATGAATTTA TTGATAGGAA AACTCCAAAC AACCCTTCCT 
     3061 GTAATACAGA TTTGATTAAT AGAATCTTGC TGGATGCGGG TTTTACAAAT GAACTTGTCC 
     3121 AAAATTATTG GAGTAAACAG AAAAATATCA AAGGAGTGAA GGCACGCTTT GTGGTGACTG 
     3181 ATGGCGGAAT TACAAGAGTT TACCCCAAAG AGGCTGGAGA AAACTGGCAA GAGAACCCAG 
     3241 AGACGTATGA GGACAGCTTC TACAAACGGA GCCTAGATAA CGATAACTAC GTTTTCACTG 
     3301 CGCCCTACTT CAACAAAAGT GGACCTGGTG CCTATGAATC TGGAATTATG GTAAGCAAAG 
     3361 CTGTAGAACT GTACATCCAA GGAAAACTTC TTAAGCCTGC AGTTGTGGGA ATTAAAATTG 
     3421 ACGTAAACTC CTGGATAGAA AATTTTACCA AAACTTCAAT CAGGGATCCG TGTGCTGGTC 
     3481 CAGTTTGTGA CTGCAAAAGA AACAGTGATG TAATGGACTG TGTCATTCTA GATGATGGTG 
     3541 GATTTCTTCT GATGGCCAAT CATGATGATT ACACTAATCA GATTGGACGT TTTTTTGGAG 
     3601 AGATTGACCC GAGCATGATG AGACACCTGG TTAATATATC ACTTTATGCA TTCAATAAAT 
     3661 CATATGACTA TCAGTCTGTG TGTGATCCAG GGGCAGCACC AAAGCAAGGA GCAGGACATC 
     3721 GCTCAGCCTA TGTGCCATCA ATTACAGACA TACTCCAGAT TGGCTGGTGG GCCACTGCTG 
     3781 CCGCCTGGTC AATTCTCCAG CAGCTACTCT TGAGTTTGAC ATTTCCACGG CTCCTTGAGG 
     3841 CAGTTGAAAT GGAAGAGGAT GACTTCACAG CTTCCCTGTC TAAGCAGAGC TGCATCACAG 
     3901 AACAAACTCA GTACTTCTTC AAGAACGATA CTAAATCATT CAGTGGTTTA CTGGACTGTG 
     3961 GAAACTGTTC CAGGATCTTT CATGTTGAGA AGCTTATGAA CACCAACCTA GTGTTCATAA 
     4021 TGGTGGAGAG CAAGGGAACA TGTCCGTGTG ACACGCGGCT GCTCATGCAA GCAGAACAGA 
     4081 CTTCTGATGG TCCAGATCCT TGCGACATGG TTAAGCAGCC CAGATACCGA AAAGGACCTG 
     4141 ATGTCTGCTT TGATAACAAT GTGCTGGAGG ATTATACCGA CTGTGGTGGT GTTTCTGGAT 
     4201 TAAACCCTTC CTTATGGTCT ATCTTTGGAC TCCAATTTAT ACTCCTTTGG CTGGTATCTG 
     4261 GCAGCAGACA CTATCTATGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4321 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4381 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4441 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4501 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4561 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4621 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4681 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4741 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4801 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4861 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4921 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4981 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5041 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5101 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5161 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5761 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5821 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5881 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5941 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6001 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6061 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6121 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6181 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6241 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6301 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6361 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6421 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6481 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6541 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6601 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6661 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6721 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6781 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6841 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6901 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6961 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7021 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7081 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7141 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7201 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7261 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7321 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7381 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7441 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7561 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7621 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7681 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7741 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7801 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7861 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7921 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7981 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8041 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8101 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//