LOCUS       RR200403                6079 bp    DNA     circular  SYN 
DEFINITION  Oprm1 (Myc-DDK-tagged ORF) - Rat opioid receptor, mu 1 (Oprm1), transcript variant 1, (10 ug)
ACCESSION   NM_013071
VERSION     NM_013071.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2236..2327 
                     /label="Myc-DDK"  
     misc_feature    2357..2942 
                     /label="PolyA signal"  
     misc_feature    2414..2433 
                     /label="Restriction Enzyme"  
     misc_feature    3218..3890 
                     /label="ColE1"  
     misc_feature    4320..5114 
                     /label="Kan/Neo"  
     misc_feature    5149..5451 
                     /label="SV40 ori"  
     misc_feature    5537..6014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2218..2224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2218 
                     /label="Oprm1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGCAGC ACCGGCCCAG GGAACACCAG CGACTGCTCA GACCCCTTAG 
     1081 CTCAGGCAAG TTGCTCCCCA GCACCTGGCT CCTGGCTCAA CTTGTCCCAC GTTGATGGCA 
     1141 ACCAGTCCGA TCCATGCGGT CTGAACCGCA CCGGGCTTGG CGGGAACGAC AGCCTGTGCC 
     1201 CTCAGACCGG CAGCCCTTCC ATGGTCACAG CCATTACCAT CATGGCCCTC TACTCTATCG 
     1261 TGTGTGTAGT GGGCCTCTTC GGAAACTTCC TGGTCATGTA TGTGATTGTA AGATACACCA 
     1321 AAATGAAGAC TGCCACCAAC ATCTACATTT TCAACCTTGC TCTGGCAGAC GCCTTAGCGA 
     1381 CCAGTACACT GCCCTTTCAG AGTGTCAACT ACCTGATGGG AACATGGCCC TTCGGAACCA 
     1441 TCCTCTGCAA GATCGTGATC TCAATAGATT ACTACAACAT GTTCACCAGC ATATTCACCC 
     1501 TCTGCACCAT GAGCGTGGAC CGCTACATTG CTGTCTGCCA CCCAGTCAAA GCCCTGGATT 
     1561 TCCGTACCCC CCGAAATGCC AAAATCGTCA ACGTCTGCAA CTGGATCCTC TCTTCTGCCA 
     1621 TCGGTCTGCC TGTAATGTTC ATGGCAACCA CAAAATACAG GCAGGGGTCC ATAGATTGCA 
     1681 CCCTCACGTT CTCCCACCCA ACCTGGTACT GGGAGAACCT GCTCAAAATC TGTGTCTTTA 
     1741 TCTTCGCTTT CATCATGCCG GTCCTCATCA TCACTGTGTG TTACGGCCTG ATGATCTTAC 
     1801 GACTCAAGAG CGTTCGCATG CTATCGGGCT CCAAAGAAAA GGACAGGAAT CTGCGCAGGA 
     1861 TCACCCGGAT GGTGCTGGTG GTCGTGGCTG TATTTATCGT CTGCTGGACC CCCATCCACA 
     1921 TCTACGTCAT CATCAAAGCG CTGATCACGA TTCCAGAAAC CACATTTCAG ACCGTTTCCT 
     1981 GGCACTTCTG CATTGCTTTG GGTTACACGA ACAGCTGCCT GAATCCAGTT CTTTACGCCT 
     2041 TCCTGGATGA AAACTTCAAG CGATGCTTCA GAGAGTTCTG CATCCCAACC TCGTCCACGA 
     2101 TCGAACAGCA AAACTCCACT CGAGTCCGTC AGAACACTAG GGAACATCCC TCCACGGCTA 
     2161 ATACAGTGGA TCGAACTAAC CACCAGCTAG AAAATCTGGA GGCAGAAACT GCTCCATTGC 
     2221 CCGCCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA 
     2281 AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT 
     2341 AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT 
     2401 GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT 
     2461 CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG 
     2521 CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG 
     2581 GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT 
     2641 CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT 
     2701 TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA 
     2761 ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC 
     2821 TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC 
     2881 ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC 
     2941 TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC 
     3001 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     3061 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     3121 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     3181 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     3241 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     3301 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     3361 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     3421 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     3481 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     3541 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     3601 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     3661 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     3721 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     3781 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     3841 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG 
     3901 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG 
     3961 AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT 
     4021 GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT 
     4081 TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT 
     4141 CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA 
     4201 TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG 
     4261 TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA 
     4321 GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG 
     4381 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC 
     4441 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA 
     4501 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC 
     4561 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC 
     4621 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC 
     4681 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC 
     4741 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG 
     4801 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT 
     4861 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA 
     4921 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC 
     4981 AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT 
     5041 TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC 
     5101 GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT 
     5161 TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG 
     5221 AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG 
     5281 CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG 
     5341 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     5401 ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     5461 GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT 
     5521 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 
     5581 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 
     5641 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 
     5701 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 
     5761 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 
     5821 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 
     5881 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 
     5941 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 
     6001 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 
     6061 AAAATTTAAC GCGAATTTT
//