LOCUS       RR200378                7641 bp    DNA     circular  SYN 
DEFINITION  Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug)
ACCESSION   NM_017241
VERSION     NM_017241.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3798..3889 
                     /label="Myc-DDK"  
     misc_feature    3919..4504 
                     /label="PolyA signal"  
     misc_feature    3976..3995 
                     /label="Restriction Enzyme"  
     misc_feature    4780..5452 
                     /label="ColE1"  
     misc_feature    5882..6676 
                     /label="Kan/Neo"  
     misc_feature    6711..7013 
                     /label="SV40 ori"  
     misc_feature    7099..7576 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3780..3786 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3780 
                     /label="Grik1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCAGC ACAGTCCTTA TCCAACCCGG GCTCTGGACC AGGGACACCA 
     1081 GCTGGACACT CCTCTATTTC CTGTGCTACA TCCTCCCTCA GACCTCCCCT CAAGTGCTCA 
     1141 GGATCGGAGG GATTTTTGAA ACTGTGGAAA ATGAACCTGT TAATGTTGAA GAATTAGCTT 
     1201 TCAAGTTTGC AGTCACCAGT ATTAACCGAA ACCGAACCTT GATGCCCAAT ACCACATTAA 
     1261 CCTATGACAT CCAGAGAATT AATCTTTTTG ATAGTTTTGA AGCCTCCCGA AGAGCATGCG 
     1321 ACCAGCTGGC TCTCGGGGTG GCCGCACTCT TCGGCCCTTC CCACAGCTCC TCCGTCAGTG 
     1381 CTGTACAGTC TATTTGCAAT GCTCTGGAAG TTCCACACAT TCAGACTCGC TGGAAACACC 
     1441 CTTCCGTGGA CAGCAGAGAC CTATTTTATA TCAACCTCTA CCCGGACTAT GCGGCTATCA 
     1501 GCAGGGCGGT CCTGGATTTG GTCCTCTATT ACAACTGGAA AACAGTGACG GTGGTGTATG 
     1561 AAGACAGCAC AGGTCTAATT CGTCTGCAAG AGCTCATCAA AGCTCCCTCC AGATACAACA 
     1621 TTAAAATCAA AATCCGCCAG CTTCCCCCTG CGAATAAAGA CGCCAAACCT CTGCTCAAGG 
     1681 AGATGAAGAA AAGCAAAGAG TTCTATGTGA TATTTGATTG TTCGCACGAA ACAGCTGCGG 
     1741 AAATTCTTAA GCAGATTTTG TTCATGGGCA TGATGACTGA ATATTATCAC TACTTCTTCA 
     1801 CAACCCTGGA CTTGTTTGCT TTAGATCTGG AACTCTATAG GTACAGCGGT GTAAATATGA 
     1861 CTGGATTTCG GTTGCTGAAT ATTGACAACC CTCACGTGTC ATCCATCATT GAGAAGTGGT 
     1921 CCATGGAGAG GTTGCAGGCC CCGCCCAGAC CCGAGACTGG TCTTCTGGAT GGCATGATGA 
     1981 CAACTGAAGC AGCGCTGATG TACGATGCTG TGTACATGGT AGCCATTGCG TCCCACCGTG 
     2041 CCTCTCAGCT GACCGTCAGC TCCCTGCAGT GCCATCGACA TAAGCCATGG CGCCTTGGAC 
     2101 CCAGATTTAT GAACCTCATC AAAGAGGCTC GGTGGGACGG CTTGACTGGG CGGATCACCT 
     2161 TCAATAAGAC CGATGGCTTG AGAAAGGATT TTGACCTGGA CATTATCAGT CTCAAAGAGG 
     2221 AAGGAACTGA AAAGGCCTCT GGTGAAGTGT CTAAACACTT GTATAAAGTG TGGAAGAAGA 
     2281 TTGGGATTTG GAACTCCAAC AGTGGGCTGA ACATGACGGA TGGCAACAGA GACAGGTCCA 
     2341 ACAATATCAC GGACTCGCTG GCTAACCGCA CACTCATTGT CACCACTATT CTGGAAGAGC 
     2401 CCTACGTGAT GTACAGGAAA TCCGATAAGC CCTTGTATGG AAACGACAGG TTTGAAGGAT 
     2461 ATTGCCTGGA TCTGCTGAAA GAACTGTCCA ATATCCTGGG TTTTCTTTAC GATGTTAAAC 
     2521 TGGTTCCTGA TGGCAAATAT GGAGCACAGA ATGACAAAGG GGAATGGAAT GGGATGGTAA 
     2581 AAGAACTCAT CGACCACAGA GCTGACCTGG CAGTGGCCCC TCTCACCATC ACATACGTAC 
     2641 GGGAGAAAGT CATTGACTTC TCCAAGCCCT TCATGACCCT GGGCATTAGC ATCCTTTACC 
     2701 GGAAGCCCAA TGGAACCAAC CCGGGTGTCT TCTCCTTCCT CAACCCCCTA TCTCCGGACA 
     2761 TTTGGATGTA CGTGCTGCTC GCCTGCCTAG GAGTCAGTTG TGTACTGTTT GTGATTGCGA 
     2821 GGTTCACACC CTACGAGTGG TATAACCCCC ACCCATGCAA CCCCGACTCA GACGTGGTGG 
     2881 AAAACAATTT CACTTTGCTA AATAGTTTCT GGTTTGGAGT TGGAGCTCTC ATGCAGCAAG 
     2941 GATCAGAGCT GATGCCCAAG GCTCTATCGA CCAGAATAGT TGGAGGAATA TGGTGGTTTT 
     3001 TCACCCTAAT CATCATTTCA TCCTACACGG CCAACCTGGC TGCCTTCTTG ACGGTAGAAA 
     3061 GAATGGAATC CCCCATCGAT TCCGCAGACG ATCTGGCCAA ACAAACCAAG ATAGAATATG 
     3121 GGGCAGTCAG AGATGGCTCG ACGATGACCT TCTTCAAGAA ATCAAAGATC TCCACCTATG 
     3181 AGAAAATGTG GGCTTTCATG AGCAGTAGAC AGCAGAGCGC ACTGGTTAAA AACAGTGACG 
     3241 AGGGGATCCA AAGGGTGCTC ACCACCGACT ACGCACTGCT GATGGAGTCC ACCAGCATTG 
     3301 AGTATGTGAC GCAGAGGAAC TGCAACCTCA CTCAGATCGG GGGCCTCATA GACTCCAAAG 
     3361 GCTATGGAGT GGGGACGCCT ATCGGCTCCC CTTACCGGGA TAAAATTACG ATTGCCATTC 
     3421 TTCAACTGCA AGAAGAAGGG AAGCTTCATA TGATGAAAGA GAAGTGGTGG AGGGGGAATG 
     3481 GCTGCCCTGA AGAAGACAGT AAGGAAGCCA GTGCTCTGGG AGTGGAAAAT ATCGGCGGCA 
     3541 TCTTCATTGT TCTGGCTGCA GGACTCGTGC TTTCTGTGTT TGTAGCCATT GGAGAATTTT 
     3601 TATACAAATC ACGGAAGAAC AATGACGTTG AGCAGTGTCT CTCTTTCAAT GCCATCATGG 
     3661 AAGAGCTGGG AATATCCCTC AAGAATCAGA AAAAATTAAA GAAAAAGTCA AGAACTAAGG 
     3721 GCAAATCTTC TTTCACAAGT ATCCTTACTT GTCACCAGAG ACGAACTCAG AGAAAAGAGA 
     3781 CAGTGGCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3841 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3901 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3961 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4021 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4081 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4141 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4201 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4261 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4321 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4381 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4441 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4501 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4561 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4621 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4681 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4741 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4801 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4861 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4921 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4981 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5041 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5101 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5161 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5221 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5281 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5341 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5401 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5461 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5521 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5581 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5641 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5701 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5761 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5821 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5881 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5941 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6001 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6061 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6121 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6181 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6241 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6301 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6361 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6421 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6481 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6541 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6601 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6661 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6721 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6781 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6841 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6901 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6961 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7081 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7141 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7201 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7261 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7321 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7381 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7441 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7501 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7561 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7621 CAAAAATTTA ACGCGAATTT T
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