LOCUS       RR200327                7359 bp    DNA     circular  SYN 
DEFINITION  Prom1 (Myc-DDK-tagged ORF) - Rat prominin 1 (Prom1), transcript variant 2, (10 ug)
ACCESSION   NM_001110137
VERSION     NM_001110137.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3516..3607 
                     /label="Myc-DDK"  
     misc_feature    3637..4222 
                     /label="PolyA signal"  
     misc_feature    3694..3713 
                     /label="Restriction Enzyme"  
     misc_feature    4498..5170 
                     /label="ColE1"  
     misc_feature    5600..6394 
                     /label="Kan/Neo"  
     misc_feature    6429..6731 
                     /label="SV40 ori"  
     misc_feature    6817..7294 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3498..3504 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3498 
                     /label="Prom1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTCGTA TTCAGTGTCC TGCTGCTACT GGGGCTGTGT GGAAAGATGG 
     1081 CTTCAGGAGG TCAGCCTGCA TTCGATAACA CTCCCGGGGC TTTGAATTAT GAATTGCCTA 
     1141 CCACAGAATA TGAGACCCAA GACACCTTCA ATGCTGGGAT TATTGACCCT CTCTACCAAA 
     1201 TGGTGCACAT CTTCCTCAAC GTGGTCCAGC CGAATGACTT CCCTCAAGAT TTGGTCAAAA 
     1261 AACTCATACA GAAGAGGTTT GACATCTCGG TTGATACCAA GGAGGTCGCC ATCTATGAAA 
     1321 TTGGAGTTCT TATCTGCGTC ATCCTGGGCC TGCTGTTCAT TTTCCTCATG CCCCTGGTGG 
     1381 GCTTCTTCTT TTGTATGTGC CGTTGCTGTA ACAAATGCGG CGGAGAAATG CACCAGCGGC 
     1441 AGAAGCAGAA CGAGTCGTGC CGGAGGAAGT GCTTAGCCAT CTCCCTCTTG CTGATTTGTC 
     1501 TGCTCATGAG TCTTGGCATC GCGTTTGGCT TTGTGGCCAA CCAGCAGACA AGGACTCGGA 
     1561 TCCAAAGGAC CCAGAAGCTG GCAGAGAGCA ATTACAGAGA CTTGCGGGCT CTCCTGACTG 
     1621 AAGCCCCAAA GCAAATCGAC TATATTCTGG GGCAATACAA CACAACCAAG AACAAGGCAT 
     1681 TCTCAGACCT GGACAGTATC GATTCCGTGC TGGGAGGCAG AATAAAGGGC CAACTAAAAC 
     1741 CCAAAGTTAC TCCTGTTCTC GAAGAGATTA AGGCCATGGC AACAGCCATT AGACAGACCA 
     1801 AGGATGCCCT GCAGAACATG AGCAGCAGTC TGAAAAGTCT ACGTGACGCA AGCACCCAGC 
     1861 TCAGTACCAA CCTGACGTCT GTGAGAAACA GCATCGAGAA TTCACTCAAC AGCAACGACT 
     1921 GTGCTTCAGA CCCAGCCAGC AAGATCTGTG ATAGCCTCAG ACCACAGCTG AGCAATCTGG 
     1981 GGAGCAACCA CAACGGAAGT CAGCTCCCAT CAGTGGACAG GGAACTCAAC ACCGTTAATG 
     2041 ATGTCGACAG AACCGATCTG GAGAGCCTCG TCAAAAGGGG GTATATGTCG ATTGATGAAA 
     2101 TACCCAATAT GATACAAAAC CAAACTGGGG ATGTCATCAA AGATGTCAAA AAGACCTTGG 
     2161 ATTCTGTTAG CTCCAAGGTT AAGAACATGA GCCAAAGTAT TCCTGTCGAG GAGGTGCTGC 
     2221 TTCAGTTCTC CCACTACCTT AATGACAGCA ACAGGTACAT CCACGAGTCG CTGCCCAGAG 
     2281 TGGAAGAATA TGACTCATAC TGGTGGCTGG GTGGCTTAAT AGTCTGCTTC CTGCTGACCC 
     2341 TCATTGTGAC TTTCTTTTAC CTGGGCTTGC TGTGTGGTGT GTTTGGCTAT GACAAGCGTG 
     2401 CCACCCCAAC CAGAAGAGGC TGCGTGTCCA ACACTGGAGG CATCTTCCTC ATGGCTGGGG 
     2461 TTGGATTCAG CTTCCTTTTT TGCTGGATAT TGATGATCCT TGTGGTTCTT ACTTTTGTTG 
     2521 TTGGTGCAAA TGTGGAAAAG TTGCTCTGTG AACCTTATGA AAACAAGAAA TTACTACAGG 
     2581 TTTTGGATAC TCCCTACCTG CTCAACGATC AATGGCAATT CTATCTCTCT GGCATACTAC 
     2641 TCAAAAACCC AGACATTAAC ATGACCTTTG AGCAAGTCTA CAGGGATTGC AAAAGAGGTC 
     2701 GAGGAGTGTA TGCCACTTTT CAGCTTGAGA ATGTCTTCAA CATCACTGAG AATTTCAACA 
     2761 TTGAGAGGCT TTCTGAAGAC ATAGTGAAGG AGTTGGAAAA GCTGAATGTG AACATTGACA 
     2821 GCATCGAACT GTTGGACAAA ACAGGAAGGA AGAGCCTCGA GGACTTTGCA CAATCTGGGA 
     2881 TAGATAGAAT CAATTATTCC ATGTACTTAC AGGAGGCTGA GAAACCCCCT ACCAAAGTGG 
     2941 ACCTGCTGAC ATTTGCCTCT TTTCTGGAAA CAGAAGCAAA CCAGTTGCCT GACGGAAATC 
     3001 TGAAACAGGC CTTCTTAATG GATGCACAGA ACATTCGAGC CATCCACCAG CAGCACGTCC 
     3061 CTCCTGTGCA GCAGTCACTG AATTCATTAA AACAAAGTGT CTGGGCCCTT AAGCAAACAA 
     3121 GCAGCAAGTT GCCGGAGGAA GTGAAGAAGG TCCTTGCCTC TTTGGATTCT GCTCAGCATT 
     3181 TCCTCACCAG TAACCTCTCC TCCATCGTTA TTGGAGAAAC AAAGAAGTTT GGGAGAACAA 
     3241 TAATAGGCTA CTTTGAACAT TATCTGCAAT GGGTCTTGTA CGCCATCACA GAGAAGATGA 
     3301 CATCCTGCAA ACCCATGATT ACAGCAATGG ACTCTGCTGT TAATGGCATT CTGTGTAGCT 
     3361 ATGTTGCTGA CCCTCTGAAT CTGTTCTGGT TCGGCATAGG GAAAGCCACC ATGCTCTTAC 
     3421 TTCCGGCTGT AATCATCGCT ATCAAGTTGG CCAAGTACTA TCGCAGGATG GATTCAGAGG 
     3481 ATGTATATGA TGACCCGTCT CGATACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3541 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3601 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3661 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3721 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3781 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3841 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3901 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3961 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4021 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4081 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4141 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4201 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4261 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4321 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4381 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4441 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4501 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4561 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4621 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4681 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4741 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4801 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4861 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4921 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4981 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5041 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5101 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5161 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5221 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5281 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5341 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5401 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5461 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5521 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5581 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5641 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5701 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5761 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5821 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5881 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5941 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6001 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6061 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6121 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6181 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6241 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6301 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6361 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6421 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6481 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6541 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6601 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6661 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6721 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6781 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6841 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6901 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6961 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7021 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7081 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7141 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7201 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7261 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7321 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//