LOCUS       RR200298                6714 bp    DNA     circular  SYN 
DEFINITION  Begain (Myc-DDK-tagged ORF) - Rat brain-enriched guanylate kinase-associated (Begain), transcript variant 2, (10 ug)
ACCESSION   NM_024163
VERSION     NM_024163.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2871..2962 
                     /label="Myc-DDK"  
     misc_feature    2992..3577 
                     /label="PolyA signal"  
     misc_feature    3049..3068 
                     /label="Restriction Enzyme"  
     misc_feature    3853..4525 
                     /label="ColE1"  
     misc_feature    4955..5749 
                     /label="Kan/Neo"  
     misc_feature    5784..6086 
                     /label="SV40 ori"  
     misc_feature    6172..6649 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2853..2859 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2853 
                     /label="Begain"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGACGGGC GGCCGGCGGC CGGGCCGGCT TCGCCGGGCG GCCTCTGCCG 
     1081 CAGACATGGA GAAACTCAGT GCGCTGCAGG AACAGAAGGG CGAGCTGCGC AAGCGCCTGT 
     1141 CCTACACCAC GCACAAGTTG GAGAAGCTGG AGACGGAGTT TGACTCCACC CGCCACTATC 
     1201 TGGAGATTGA GCTGAGGCGC GCACAGGAGG AGCTGGACAA GGTCACCGAG AAACTGCGCA 
     1261 GGATTCAGAG CAACTACATG GCACTCCAGA GGATCAACCA AGAGCTGGAA GACAAGCTGT 
     1321 ACCGGATGGG CCAGCACTAT GAGGAAGAGA AGCGTGCCAT GAGCCACGAG ATTGTCGCCC 
     1381 TCAACAGCCA CCTGCTGGAG GCTAAGGTGA CCATTGACAA GCTGTCAGAA GACAACGAGC 
     1441 TCTATAGGAA GGACTGCAAT CTAGCGGCCC AGCTGCTGCA GTGCAGCCAG ACCTACGGCA 
     1501 GGGTCCATAA GGTGTCCGAG CTGCCCTCGG ACTTCCAGCA GCGTGTGAGC CTGCATATGG 
     1561 AGAAGCATGG CTGCAGCCTG CCGTCCCCAC TGTGCCATCC GTCCTACGCC GACAGCGTGC 
     1621 CCACCTGCGT CATCGCCAAG GTGCTGGAGA AGCCCGACCC TGGCAGCCTG TCCTCGCGCA 
     1681 TGTCGGATGC CTCGGCCCGC GACCTGGCCT ACCGCGACGG AGTGGAGAAC CCGGGCCCGC 
     1741 GACCCCCGTA CAAGGGAGAC ATCTACTGCA GCGACACGGC TCTCTACTGC CCTGACGAGC 
     1801 GAGATCACGA CCGGCGGCCC AGCGTGGACA CGCCGGTGAC CGACGTGGGC TTCCTGCGTG 
     1861 CGCAGAATTC CACCGACAGC CTGGCGGAAG AGGAGGAGGC CGAGGCGGCG GCCTTCCCGG 
     1921 AGGCCTACCG TCGCGAGGCC TTCCAGGGCT ACGCGGCCTC GCTGCCCACG TCCAGCTCCT 
     1981 ACTCCAGCTT CAGCGCCACG TCCGAGGAGA AGGAGCACGC GCAGGCCAGC ACGCTGACCG 
     2041 CCTCGCAGCA GGCCATCTAC CTGAACAGCC GCGAAGAGCT CTTCAGCCGC AAGCCGCCCT 
     2101 CCGCCACCTA CGGCAGCAGC CCTCGCTACG CCAAGGCCGC GGCCACCCTG GGCTCCCCGC 
     2161 TCGAGGCCCA GGTAGCCCCA GGCTTCGCTC GGACTGTGTC TCCGTACCCG GCCGAGCCCT 
     2221 ACCGCTATCC GGCCTCCCAG CAGGCTCTCA TGCCTCCCAA CCTGTGGAGC CTGCGGGCCA 
     2281 AGCCGAGCGG TAACCGGCTT GCCGCCCGGG AGGACATTCG AGGCCAGTGG CGGCCCCTGA 
     2341 GCGTGGAGGA TGTGGGCGCC TACTCTTACC AGGCCGGCGC TGCAGGCCGC GCCGCCTCGC 
     2401 CCTGCAACTT CTCAGAACGT TTCTACGGCG GCGGTGGCGG CGGCGGCAGC CCGGGCAAGA 
     2461 ATGCCGAGGG CCGTGCCAGC CCCCTCTATG CCAGCTACAA AGCCGATAGT TTCTCGGAGG 
     2521 GCGATGACCT CTCCCAGGGT CATCTGGCCG AGCCCTGCTT CCTCCGAGCG GGTGGTGATC 
     2581 TGAGCCTCAG CCCCAGCCGT TCAGCTGATC CTCTCCCTGG CTATGCCACC AGTGACGGGG 
     2641 ATGGGGATAG GCTCGGGGTG CAGCTGTGTG GGCCTGGCAG TAGCCCGGAG CCCGAGCACG 
     2701 GCTCCCGGGA TTCCCTGGAG CCTAGCTCCA TGGAGGCCTC TCCCGAAATG CACCCTCCAA 
     2761 CCCGCCTCAG CCCCCAGCAG GCCTTCCCAA GGACTGGAGG CTCTGGGCTG AGCCGCAAGG 
     2821 ACAGTCTCAC TAAGGCCCAG CTCTACGGAA CCCTGCTCAA CACGCGTACG CGGCCGCTCG 
     2881 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2941 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3001 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3061 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3121 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3181 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3241 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3301 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3361 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3421 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3481 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3541 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3601 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3661 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3721 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3781 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3841 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3901 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3961 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4021 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4081 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4141 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4201 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4261 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4321 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4381 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4441 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4501 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4561 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4621 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4681 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4741 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4801 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4861 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4921 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4981 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5041 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5101 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5161 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5221 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5281 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5341 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5401 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5461 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5521 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5581 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5641 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5701 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5761 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5821 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5881 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5941 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6001 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6061 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6121 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6181 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6241 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6301 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6361 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6421 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6481 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6541 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6601 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6661 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//