LOCUS       RR200286                7620 bp    DNA     circular  SYN 
DEFINITION  Lig1 (Myc-DDK-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug)
ACCESSION   NM_001024268
VERSION     NM_001024268.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="Lig1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAGAAGT ATTATGTCAT TTTTCCAACC CACAACAACA GAAGGTAAAG 
     1081 CGAAGAAGCC AGAGAAGGAG ATACCCAGCA GCATCAGAGA GAAGGAACCC CCTCCAAAGG 
     1141 TGGCATTGAA GGAGAGGAAT CGAGCAGTGC CTGAGAGTGA TTCTCCAGTG AAGAGGCCAG 
     1201 GAAGGAAGGT GGCCCAGGTT CTGAGCAGTG AAGGGGAGGA CGAAGATGAA GCCCCTGGCA 
     1261 CCCCCCAAGT CCAAAAGCCT GTGTCAGACT CCAAACAGAG CTCTCCTCCC AGCCCTGACT 
     1321 CCTGTCCTGA GAACAGCAGC CCCTCCATGG ACATCTCCCC ATCAGGATTC CCAAAGCGCC 
     1381 GAACTGCACG GAAGCAGCTC CCAAAACGGA CAATTCAAGA CACTCTTGAG GAGCACAATG 
     1441 AAGACAAAGA CAAAGCAGCT AAGAAAAGGA AAAAAGAAGA TACACAGACC CCAACAGAAA 
     1501 GCCTCACAGA GGCTGAAGAA GTAAAACAGA AGGAAGAACA GGTGGAGGAC CAGCCCACAG 
     1561 TACCCCCTGA GCCCACAGAG TCCCCTGAGT CAGTAACCCT CACAAAGACA GAGAACATTC 
     1621 CAATGTGTAA GGCAGGTGTG AAACAGAAGC CTCAGGAAGA AGAACAGAGC AAGCCTCCTG 
     1681 CCAGGGGTGC CAAGCCACTG AGCAGTTTCT TCACACCCCG AAAACCAGCA GTGAAAACTG 
     1741 AAGTCAAACA AGAAGAGTCA GACACTCCAA GGAAGGAAGA GACCAAGGGA GCCCCGGATC 
     1801 CAACCAATTA TAATCCTTCC AAGAGTAACT ACCATCCCAT TGAAGATGCC TGCTGGAAAC 
     1861 ATGGCCAAAA GGTTCCTTTT CTTGCTGTGG CCCGGACATT TGAGAAGATT GAGGAGGTGT 
     1921 CTGCTCGGCT TAAGATGGTG GAGACACTGA GCAACCTGCT GCGCTCCGTG GTGGCCCTGT 
     1981 CCCCAACAGA CCTGCTCCCT GTTCTCTATC TCAGCCTCAA CCGCCTTGGG CCGCCTCAGC 
     2041 AGGGTCTAGA ACTGGGTGTT GGTGATGGTG TCCTCCTCAA GGCAGTTGCC CAGGCCACAG 
     2101 GTCGTCAGCT GGAGTCCATC CGGGCTGAGG TAGCTGAGAA GGGGGATGTG GGGCTAGTGG 
     2161 CGGAGAACAG CCGAAGCACT CAGAGGCTCA TGCTGCCTCC ACCTCCACTT ACTGTTTCTG 
     2221 GAGTCTTTAC CAAATTCTGT GACATTGCCC GGCTCACTGG CAGTGCTTCC ATGGCCAAGA 
     2281 AAATGGACAT TATCAAGGGC CTTTTTGTTG CCTGCCGCTA CTCAGAAGCC CGGTTTATTG 
     2341 CCAGGTCCCT AAGTGGGCGG CTGCGCCTTG GGCTGGCTGA GCAGTCAGTA CTGGCTGCTC 
     2401 TTGCCCAGGC TGTGAGCCTC ACACCCCCTG GCCAAGAATT TCCCACAGCC GTGGTGGATG 
     2461 CTGGGAAGGG CAAGACAGCA GAGGCCAGAA AGATGTGGTT GGAAGAACAA GGCATGATCT 
     2521 TGAAGCAAAC CTTCTGTGAG GTGCCTGATC TGGATCGCAT CATCCCTGTG CTGCTGGAAC 
     2581 ATGGCTTGGA AAGTCTCCCA GAGCACTGCA AGCTGAGCCC AGGGGTTCCT CTTAAACCAA 
     2641 TGCTGGCCCA TCCCACTCGG GGTGTCCGTG AGGTCCTGAA ACGCTTTGAG GAGGTCGACT 
     2701 TCACCTGCGA GTATAAATAC GATGGGCAGC GGGCACAGAT ACATGTTCTG GAAGGTGGAG 
     2761 AAGTGAAGAT CTTCAGCAGG AACCAGGAAG ACAACTCAGG AAAGTATCCT GACATCATCA 
     2821 GCCGCATCCC CAAGATTAAA CACCCCTCAG TCACATCTTT CATTCTGGAC ACTGAGGCTG 
     2881 TGGCCTGGGA CCGGGAAAAG AAGCAGATCC AGCCATTCCA AGTGCTCACC ACACGCAAGC 
     2941 GCAAGGAGGT TGATGCATCG GAGATACAGG TACAAGTGTG TCTGTATGCT TTTGACCTCA 
     3001 TCTACCTCAA CGGAGAGTCC CTGGCTCGCC AGCCCCTGTC TCGACGCCGG CAGCTACTCC 
     3061 GTGAGAACTT TGTGGAGACA GAGGGTGAGT TTGTCTTTGC CACCTCCTTG GACACCAAGG 
     3121 ACATCGAGCA GATTGCCGAG TTCTTAGAGC AGTCAGTGAA GGACTCCTGT GAGGGCCTGA 
     3181 TGGTGAAGAC CCTGGATGTT GATGCTACCT ATGAGATCGC CAAGAGGTCA CACAACTGGC 
     3241 TCAAGCTGAA GAAGGACTAC CTTGAAGGTG TGGGCGACAC TCTAGACCTT GTGGTGATTG 
     3301 GTGCCTACCT GGGCCGGGGG AAGCGGGCTG GCCGGTATGG GGGCTTCCTG TTGGCTGCCT 
     3361 ACGATGAGGA GAGTGAAGAG CTGCAGGCCA TATGCAAGCT GGGAACCGGA TTCAGCGACG 
     3421 AGGAGCTGGA AGAACATCAT CAGAGCATGC AGGCCCTTTT ATTGCCTACC CCACGCCCCT 
     3481 ATGTGAGGAT TGATGGGGCA GTGGCCCCAG ACCACTGGCT AGACCCGAGT GTTGTGTGGG 
     3541 AGGTGAAATG TGCTGATCTC TCCCTGTCTC CCATCTACCC TGCTGCTCGG GGCCTGGTGG 
     3601 ACAAAGAAAA AGGGATCTCC CTTCGTTTCC CTCGGTTCAT TCGTGTCCGT GAGGATAAGC 
     3661 AGCCAGAGCA GGCCACTACC AGTGACCAGG TGGCCTCTTT GTACCGGAAG CAGAGTCAGA 
     3721 TTCAGAACCA GCAAAGCTCA GACTTGGACT CTGATGTTGA AGATTACACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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