LOCUS       RR200272                7695 bp    DNA     circular  SYN 
DEFINITION  Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug)
ACCESSION   NM_001143847
VERSION     NM_001143847.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3850..3941 
                     /label="Myc-DDK"  
     misc_feature    3971..4556 
                     /label="PolyA signal"  
     misc_feature    4028..4047 
                     /label="Restriction Enzyme"  
     misc_feature    4832..5504 
                     /label="ColE1"  
     misc_feature    5934..6728 
                     /label="Kan/Neo"  
     misc_feature    6763..7065 
                     /label="SV40 ori"  
     misc_feature    7151..7628 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3825..3832 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3825 
                     /label="Pde2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCAGGCA TGCGGCCACT CCATCCTCTG CAGGAGCCAG CAGTACCCGG 
     1081 CAGCGCGACC GGCTGAGCCG CGGGGCCAGC AGGTCTTCCT CAAGCCGGAC GAACCGCCGC 
     1141 CGCAGCCATG CGCCGACAGC CTGCAGGATG CTTTGCTGAG CCTGGGCGCC GTTATCGACA 
     1201 TCGCGGGCCT TCGACAGGCT GCCAAGGATG CCCTCTCGGC CGTGCTCCCC AAAGTGGAGA 
     1261 CTGTCTACAC CTACCTGGTA GATGGGGAGT CCAGGCTGGT GTGTGAGGAC CCCCCTCATG 
     1321 AGCTGCCACA GGAAGGAAAA ATTCGAGAGG CTGTGATTTC TCGGAAGCGG CTGAGCTGCG 
     1381 ATGGGCTGGG ACCTTCAGAC CTACTAGGAA AGCCCTTGGC CAGGCTGGTG GCTCCACTGG 
     1441 CCCCTGACAC GCAAGTGCTG GTCATACCCC TGCTGGACAA GGAGACTGGA ACTGTGGCAG 
     1501 CCGTCATCTT GGTGCACTGT GGCCAGCTGA GTGACAGTGA GGAGCAGAGC CTGCAGGTGG 
     1561 TGGAGAAGCA TGCTCTGGTG GCCCTGCAGA GGGTGCAGGC CCTGCAGCAG CGCAGGCCTG 
     1621 AAGCTGTTCA GAACACCTCA GCGGATCCCT CTGAAGATCA AAAGGATGAG AAGGGATACA 
     1681 CCGCCCATGA CCGAAAGATC CTGCAACTGT GTGGAGAACT CTATGACTTG GATGCCACTT 
     1741 CTCTGCAGCT CAAAGTCCTT CGATATCTTC AGCAGGAGAC ACAGGCCACT CACTGCTGCC 
     1801 TCCTGCTGGT GTCAGAGGAC AACCTGCAGC TTTCCTGCAA GGTCATTGGA GAGAAAGTGC 
     1861 TGGGAGAAGA GGTCAGCTTT CCATTGACCA TGGGACGCCT GGGCCAGGTG GTGGAGGACA 
     1921 AACAGTGTAT CCAGTTGAAG GACCTAACCT CTGACGATGT GCAACAGCTA CAAAACATGT 
     1981 TGGGCTGTGA GCTTCGGGCT ATGCTATGTG TCCCTGTCAT CAGTCGAGCC ACTGACCAGG 
     2041 TGGTGGCCCT GGCTTGCGCC TTCAACAAGC TTGGAGGAGA CTTCTTCACA GATGAGGATG 
     2101 AACGTGCGAT CCAACACTGC TTCCACTACA CAGGCACGGT GCTCACCAGT ACCTTGGCCT 
     2161 TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG GCAAAGAACC 
     2221 TCTTCACCCA CCTGGATGAC GTCTCTGTCC TGCTACAGGA GATCATCACA GAGGCCAGAA 
     2281 ACCTCAGCAA CGCAGAGATC TGCTCCGTGT TCCTGCTGGA TCAGAATGAG CTGGTTGCCA 
     2341 AGGTGTTCGA TGGCGGTGTA GTGGACGATG AGAGTTATGA GATCCGCATC CCTGCGGACC 
     2401 AAGGCATCGC GGGCCACGTG GCGACCACGG GCCAGATCCT GAACATCCCA GATGCATACG 
     2461 CCCATCCGCT TTTCTATCGC GGCGTAGACG ACAGCACTGG CTTCCGCACG CGCAACATTC 
     2521 TCTGCTTCCC TATCAAGAAC GAGAACCAAG AGGTCATCGG TGTGGCTGAG CTAGTGAACA 
     2581 AGATCAATGG GCCATGGTTC AGCAAATTTG ATGAGGACCT GGCCACAGCC TTCTCCATCT 
     2641 ACTGTGGCAT TAGCATCGCT CACTCTCTCC TATACAAAAA GGTGAATGAA GCCCAGTACC 
     2701 GCAGCCACCT GGCCAACGAG ATGATGATGT ACCATATGAA GGTCTCTGAC GATGAATACA 
     2761 CCAAACTTCT CCATGATGGC ATCCAACCTG TGGCCGCCAT TGACTCCAAC TTTGCCAACT 
     2821 TCACCTATAC TCCTCGGTCT CTGCCTGAGG ACGATACTTC TATGGCCATC CTGAGCATGC 
     2881 TGCAAGACAT GAACTTCATC AATAACTACA AAATTGACTG CCCAACTCTG GCCCGGTTCT 
     2941 GCCTGATGGT GAAGAAAGGC TACCGGGATC CGCCCTACCA CAACTGGATG CACGCCTTCT 
     3001 CTGTCTCTCA CTTTTGCTAC TTGCTCTACA AGAATCTGGA GCTCTCCAAC TACCTCGAGG 
     3061 ACATCGAGAT CTTTGCGTTG TTTATTTCCT GCATGTGTCA CGACCTGGAC CACAGAGGCA 
     3121 CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC TGCACTCTAC AGCTCAGAGG 
     3181 GCTCTGTCAT GGAGAGGCAC CACTTTGCTC AGGCCATTGC TATCCTCAAC ACCCATGGCT 
     3241 GTAATATCTT TGACCACTTC TCTCGGAAGG ACTATCAGCG AATGCTGGAC CTGATGAGGG 
     3301 ACATCATCTT GGCCACGGAC CTGGCACACC ACCTCCGCAT CTTCAAGGAC CTGCAGAAGA 
     3361 TGGCTGAAGT GGGTTATGAC CGAAACAACA AGCAACACCA CAGGCTTCTT CTATGCCTCC 
     3421 TTATGACCTC CTGTGACCTC TCTGACCAGA CAAAGGGCTG GAAGACCACC AGAAAGATTG 
     3481 CGGAGCTGAT CTACAAAGAG TTCTTCTCCC AAGGAGACTT GGAGAAGGCC ATGGGCAACC 
     3541 GACCAATGGA GATGATGGAT CGGGAGAAGG CCTACATCCC TGAACTTCAG ATCAGCTTTA 
     3601 TGGAGCACAT CGCCATGCCC ATCTACAAGC TGTTACAAGA CCTGTTCCCC AAGGCAGCAG 
     3661 AGCTGTATGA ACGCGTGGCC TCCAATCGTG AGCACTGGAC CAAGGTGTCC CACAAGTTCA 
     3721 CCATCCGAGG CCTCCCCAGT AACAACTCGC TGGATTTCCT GGATGAGGAA TATGAGGTCC 
     3781 CTGATTTGGA CGTCACCAGG GCTCCTGTCA ATGGCTGCTG CAGCCTCGAG GGCAGCGGAC 
     3841 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3901 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3961 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4021 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4081 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4141 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4201 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4261 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4321 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4381 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4441 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4501 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4561 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4621 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4681 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4741 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4801 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4861 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4921 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4981 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5041 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5101 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5161 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5221 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5281 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5341 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5401 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5461 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5521 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5581 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5641 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5701 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5761 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5821 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5881 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5941 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6001 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6061 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6121 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6181 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6241 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6301 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6361 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6421 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6481 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6541 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6601 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6661 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6721 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6781 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6841 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6901 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7021 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7081 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7141 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7201 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7261 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7321 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7381 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7441 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7501 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7561 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7621 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7681 TTTAACGCGA ATTTT
//