LOCUS       RR200257                8625 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged ORF) - Rat synaptojanin 2 (Synj2), transcript variant 3, (10 ug)
ACCESSION   NM_032071
VERSION     NM_032071.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGAGC AAAGGGCTGC GACTGCTGGC GCGGCTGGAC CCCACCGGCC 
     1081 CGAGCAGCGT GCTGCTGGAG GCGCGGGGCC GCGGCGACTG TCTACTCTTT GAGGCCGGCG 
     1141 CGGTGGCCAC GCTGGCTCCG GAAGAGAAGG AAGTCATTAA AGGACTGTAT GGGAAGCCGA 
     1201 CGGATGCCTA TGGCTGCCTC GGGGAGCTGA GTTTAAAATC CGGTGGCGTC CCCTTGAGCT 
     1261 TTCTGGTGTT GGTGACAGGC TGCACGTCAG TGGGCAGAAT TCCAGATGCA GAGATCTACA 
     1321 AAATCACTGG CACTGAGTTT TACCCCCTGC AGGAAGAGGC CAAGGAAGAG GACCGCCTGC 
     1381 CAGCCTTGAA GAAAATCCTG AGTTCAGGGG TGTTCTATTT CGCATGGCCC AATGATGGCG 
     1441 CCTGCTTCGA TCTGACCATC AGGGCTCAGA AACAGGGTGA TGACTGCTCT GAATGGGGGA 
     1501 CCTCCTTCTT CTGGAACCAG CTATTGCATG TGCCTCTGCG GCAGCACCAG GTGAACTGTC 
     1561 ATGACTGGTT GCTGAAAGTC ATCTGTGGGG TGGTGACCAT CCGCACGGTT TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGT CTCATCTCTC GCATCAGCTG TGAACGCGCA GGTGCTCGCT 
     1681 TCCTCACCCG GGGTGTGAAC GACGATGGCC ACGTGTCCAA CTTTGTGGAG ACAGAGCAGG 
     1741 CGATTTACAT GGATGATGGA GTGTCGTCCT TTGTCCAGAT CCGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAGCA GCCAGGGCTC CAGGTTGGCT CCCATCATCT GAGACTGCAC AGAGGCCTAG 
     1861 AGGCCAACGC CCCTGCTTTT GAAAGGCACA TGGTGCTTCT GAAGGAGCAG TACGGGCAGC 
     1921 AGGTGGTGGT GAACCTGCTG GGAAGCAGAG GCGGTGAGGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGTTGCT CTGGGCTTCT TGCCACGCAG GCGACACGCC TATGATAAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTAGG AAGCTAGAGA AATTGGAGAA CCTGTTGAGA CCTCAGTTAA 
     2101 AACTACACTG GGATGACTTT GGCGTATTTG CGAAGGGCGA GAATGTAAGT CCACGGTTTC 
     2161 AGAAAGGCAC TCTGCGGATG AACTGTCTCG ACTGTCTGGA CAGAACCAAC ACTGTACAGT 
     2221 GCTTCATTGC TCTTGAGGTG CTTCATCTGC AGCTTGAGAG CCTAGGGCTG AATTCCAAGC 
     2281 CCATCACTGA CCGCTTCGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGGCATGGCC 
     2341 TGAGCAAGGT GTTCACAGGG AGCAGGGCCC TGGAAGGAAA GGCCAAGGTG GGGAAGCTGA 
     2401 AGGATGGGGC TAGATCCATG TCTCGTACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTACTG CTAGTTGGAG ATGTCTACAA TGAAGAGTCT ACAGACAAAG 
     2521 GCAGGATGCT GCTGGACAAC ACGGCCCTTC TGGGTTTGGG GTCAAATAAA CAAAACAGTC 
     2581 TTTCAGGCAT GCTGGATGGA AAAGCAACAC CCAGGATCCT GAAGGCCATG ACAGAACGCC 
     2641 AGTCAGAATT CACAAATTTC AAGCGGATCC AGATTGCTAT GGGGACCTGG AATGTGAACG 
     2701 GAGGAAAACA ATTCCGTAGC AATCTCCTGG GGACCACTGA ACTCACTGAC TGGCTCCTGG 
     2761 ATGCACCCCA GCTCTCAGGA GCAGTGGACT CTCAGGACGA TGGCGGTCCC GCTGACATAT 
     2821 TTGCCGTCGG GTTTGAGGAG ATGGTGGAGC TGAGTGCAGG AAATATTGTC AATGCCAGCA 
     2881 CCACCAACAG GAAGATGTGG GGTGAGCAGC TCCAGAAAGC CATCTCCCGA TCCCATCGGT 
     2941 ACATCCTCTT GACCTCAGCA CAGCTGGTGG GCGTCTGTCT TTACATCTTT GTACGTCCGT 
     3001 ACCACGTCCC GTTCATCAGG GATGTGGCCA TCGACACCGT GAAGACCGGT ATGGGGGGAA 
     3061 AGGCAGGGAA TAAGGGCGCC GTGGGTATCC GCTTTCAGTT CCACAGCACT AGCTTCTGCT 
     3121 TCATCTGCAG TCACCTGACG GCTGGGCAGT CTCAGGTGAA GGAGAGGAAC GAAGACTACC 
     3181 GGGAGATCAC GCACAAGCTC TCCTTCCCTT CGGGGAGAAA CATATTTTCA CACGATTATG 
     3241 TGTTTTGGTG TGGCGATTTC AACTACCGCA TCGATCTTAC TTACGAAGAA GTCTTCTATT 
     3301 TTGTTAAACG CCAAGACTGG AAGAAACTTA TGGAATTTGA TCAGTTACAG CTACAGAAAT 
     3361 CAAGTGGGAA AATTTTTAAA GACTTTCATG AAGGAACCAT TAACTTCGGA CCCACCTACA 
     3421 AGTATGACGT TGGATCGGCT GCCTATGACA CAAGTGACAA GTGCCGGACC CCAGCCTGGA 
     3481 CAGACAGGGT GCTGTGGTGG AGGAAGAAGC ATCCGTACGA TAAGACAGCT GGCGAACTCA 
     3541 ACCTTCTAGA CAGTGATCTA GACGGTGATG CCAACATCAG ACATACCTGG TCTCCGGGCA 
     3601 CTCTCAAGTA CTACGGCCGT GCCGAGCTGC AGGCATCTGA TCACAGACCT GTGCTGGCCA 
     3661 TCGTAGAGGT GGAGGTTCAA GAGGTCGATG TGGGAGCCCG GGAGAGAGTC TTCCAGGAAG 
     3721 TGTCCTCTGT CCAAGGCCCG CTGGACGCCA CTGTTATTGT AAACCTTCAG TCTCCAACCC 
     3781 TTGAAGAGAG AAATGAATTT CCAGAGGACC TGCGCACAGA ACTTATGCAG ACTTTGGGGA 
     3841 ATTATGGGAC GATTATTCTG GTCAGGATCA ACCAAGGGCA GATGCTGGTG ACATTTGCAG 
     3901 ACAGCCACTC AGCTCTCAGT GTGCTGGATG TGGATGGTAT GAAGGTGAAA GGCAGGGCCG 
     3961 TGAAGATTCG ACCAAAGACC AAAGATTGGC TAGAAGGCTT GAGAGAGGAG CTCATTCGGA 
     4021 AGCGGGACAG CATGGCCCCT GTGTCTCCCA CCGCCAACTC CTGCTTGTTG GAGGAGAACT 
     4081 TTGACTTCAC AAGTCTGGAC TATGAGTCAG AAGGGGATGT TCTTGAAGAT GATGAAGACT 
     4141 ACTTAGCGGA TGAGTTTGGT CAGCCTGTAG TCTCAGACAG TGAGCTTGGA GGAGACGACT 
     4201 CTTCTGATAC CATGAGCGCC TCGACACCTG CCAGCAAGTC TCCCGCACTG GCTAAAAAGA 
     4261 AGCAACATCC AACGTACAAA GCTGGCTTAA TGGTGAAGAA GTCTGCCTCA GATGCGTCTA 
     4321 TCTCCTCCGG AACCCACGGG CAATATTCCA TCTTGCAGAC CGCGAAACTT CTTCCAGGAG 
     4381 CACCTCAGCA ACCGCCCAAG GCTCGAACTG GAATAAGTAA ACCTTACAAC GTCAAACAGA 
     4441 TCAAAACCAC CAATGCTCAG GAGGCGGAAG CAGCTATCCG GTGTCTCCTG GAAGCTGGCG 
     4501 GAGGTGTCCC AGAGTCAGCC CCGGGTGCCA CGCCCCTGAG AAACCAAGGG TCTTCTAAGC 
     4561 CAGAGGCCTC CCTGGGGCCT CCAGTCCTAC CGCGCCGGCC TGTTCCAAGG GTTCCCACTA 
     4621 TGAAGAAACC AACTTTGAGG AGAACAGGAA AGATCGTTTT CTGCTCTAGC TCACAAGCTT 
     4681 CTCAACCTTG CTCACTGCTG CAAAGACATG AGTTTGTAAG GACAGTAGCC GCCCAGAGAC 
     4741 TGACACCCGT AGATGCTTCC GGATCTTCCG TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//