LOCUS       RR200256                9234 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged ORF) - Rat synaptojanin 2 (Synj2), transcript variant 2, (10 ug)
ACCESSION   NM_001113372
VERSION     NM_001113372.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5391..5482 
                     /label="Myc-DDK"  
     misc_feature    5512..6097 
                     /label="PolyA signal"  
     misc_feature    5569..5588 
                     /label="Restriction Enzyme"  
     misc_feature    6373..7045 
                     /label="ColE1"  
     misc_feature    7475..8269 
                     /label="Kan/Neo"  
     misc_feature    8304..8606 
                     /label="SV40 ori"  
     misc_feature    8692..9169 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5373..5379 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5373 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGAGC AAAGGGCTGC GACTGCTGGC GCGGCTGGAC CCCACCGGCC 
     1081 CGAGCAGCGT GCTGCTGGAG GCGCGGGGCC GCGGCGACTG TCTACTCTTT GAGGCCGGCG 
     1141 CGGTGGCCAC GCTGGCTCCG GAAGAGAAGG AAGTCATTAA AGGACTGTAT GGGAAGCCGA 
     1201 CGGATGCCTA TGGCTGCCTC GGGGAGCTGA GTTTAAAATC CGGTGGCGTC CCCTTGAGCT 
     1261 TTCTGGTGTT GGTGACAGGC TGCACGTCAG TGGGCAGAAT TCCAGATGCA GAGATCTACA 
     1321 AAATCACTGG CACTGAGTTT TACCCCCTGC AGGAAGAGGC CAAGGAAGAG GACCGCCTGC 
     1381 CAGCCTTGAA GAAAATCCTG AGTTCAGGGG TGTTCTATTT CGCATGGCCC AATGATGGCG 
     1441 CCTGCTTCGA TCTGACCATC AGGGCTCAGA AACAGGGTGA TGACTGCTCT GAATGGGGGA 
     1501 CCTCCTTCTT CTGGAACCAG CTATTGCATG TGCCTCTGCG GCAGCACCAG GTGAACTGTC 
     1561 ATGACTGGTT GCTGAAAGTC ATCTGTGGGG TGGTGACCAT CCGCACGGTT TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGT CTCATCTCTC GCATCAGCTG TGAACGCGCA GGTGCTCGCT 
     1681 TCCTCACCCG GGGTGTGAAC GACGATGGCC ACGTGTCCAA CTTTGTGGAG ACAGAGCAGG 
     1741 CGATTTACAT GGATGATGGA GTGTCGTCCT TTGTCCAGAT CCGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAGCA GCCAGGGCTC CAGGTTGGCT CCCATCATCT GAGACTGCAC AGAGGCCTAG 
     1861 AGGCCAACGC CCCTGCTTTT GAAAGGCACA TGGTGCTTCT GAAGGAGCAG TACGGGCAGC 
     1921 AGGTGGTGGT GAACCTGCTG GGAAGCAGAG GCGGTGAGGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGTTGCT CTGGGCTTCT TGCCACGCAG GCGACACGCC TATGATAAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTAGG AAGCTAGAGA AATTGGAGAA CCTGTTGAGA CCTCAGTTAA 
     2101 AACTACACTG GGATGACTTT GGCGTATTTG CGAAGGGCGA GAATGTAAGT CCACGGTTTC 
     2161 AGAAAGGCAC TCTGCGGATG AACTGTCTCG ACTGTCTGGA CAGAACCAAC ACTGTACAGT 
     2221 GCTTCATTGC TCTTGAGGTG CTTCATCTGC AGCTTGAGAG CCTAGGGCTG AATTCCAAGC 
     2281 CCATCACTGA CCGCTTCGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGGCATGGCC 
     2341 TGAGCAAGGT GTTCACAGGG AGCAGGGCCC TGGAAGGAAA GGCCAAGGTG GGGAAGCTGA 
     2401 AGGATGGGGC TAGATCCATG TCTCGTACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTACTG CTAGTTGGAG ATGTCTACAA TGAAGAGTCT ACAGACAAAG 
     2521 GCAGGATGCT GCTGGACAAC ACGGCCCTTC TGGGTTTGGG GTCAAATAAA CAAAACAGTC 
     2581 TTTCAGGCAT GCTGGATGGA AAAGCAACAC CCAGGATCCT GAAGGCCATG ACAGAACGCC 
     2641 AGTCAGAATT CACAAATTTC AAGCGGATCC AGATTGCTAT GGGGACCTGG AATGTGAACG 
     2701 GAGGAAAACA ATTCCGTAGC AATCTCCTGG GGACCACTGA ACTCACTGAC TGGCTCCTGG 
     2761 ATGCACCCCA GCTCTCAGGA GCAGTGGACT CTCAGGACGA TGGCGGTCCC GCTGACATAT 
     2821 TTGCCGTCGG GTTTGAGGAG ATGGTGGAGC TGAGTGCAGG AAATATTGTC AATGCCAGCA 
     2881 CCACCAACAG GAAGATGTGG GGTGAGCAGC TCCAGAAAGC CATCTCCCGA TCCCATCGGT 
     2941 ACATCCTCTT GACCTCAGCA CAGCTGGTGG GCGTCTGTCT TTACATCTTT GTACGTCCGT 
     3001 ACCACGTCCC GTTCATCAGG GATGTGGCCA TCGACACCGT GAAGACCGGT ATGGGGGGAA 
     3061 AGGCAGGGAA TAAGGGCGCC GTGGGTATCC GCTTTCAGTT CCACAGCACT AGCTTCTGCT 
     3121 TCATCTGCAG TCACCTGACG GCTGGGCAGT CTCAGGTGAA GGAGAGGAAC GAAGACTACC 
     3181 GGGAGATCAC GCACAAGCTC TCCTTCCCTT CGGGGAGAAA CATATTTTCA CACGATTATG 
     3241 TGTTTTGGTG TGGCGATTTC AACTACCGCA TCGATCTTAC TTACGAAGAA GTCTTCTATT 
     3301 TTGTTAAACG CCAAGACTGG AAGAAACTTA TGGAATTTGA TCAGTTACAG CTACAGAAAT 
     3361 CAAGTGGGAA AATTTTTAAA GACTTTCATG AAGGAACCAT TAACTTCGGA CCCACCTACA 
     3421 AGTATGACGT TGGATCGGCT GCCTATGACA CAAGTGACAA GTGCCGGACC CCAGCCTGGA 
     3481 CAGACAGGGT GCTGTGGTGG AGGAAGAAGC ATCCGTACGA TAAGACAGCT GGCGAACTCA 
     3541 ACCTTCTAGA CAGTGATCTA GACGGTGATG CCAACATCAG ACATACCTGG TCTCCGGGCA 
     3601 CTCTCAAGTA CTACGGCCGT GCCGAGCTGC AGGCATCTGA TCACAGACCT GTGCTGGCCA 
     3661 TCGTAGAGGT GGAGGTTCAA GAGGTCGATG TGGGAGCCCG GGAGAGAGTC TTCCAGGAAG 
     3721 TGTCCTCTGT CCAAGGCCCG CTGGACGCCA CTGTTATTGT AAACCTTCAG TCTCCAACCC 
     3781 TTGAAGAGAG AAATGAATTT CCAGAGGACC TGCGCACAGA ACTTATGCAG ACTTTGGGGA 
     3841 ATTATGGGAC GATTATTCTG GTCAGGATCA ACCAAGGGCA GATGCTGGTG ACATTTGCAG 
     3901 ACAGCCACTC AGCTCTCAGT GTGCTGGATG TGGATGGTAT GAAGGTGAAA GGCAGGGCCG 
     3961 TGAAGATTCG ACCAAAGACC AAAGATTGGC TAGAAGGCTT GAGAGAGGAG CTCATTCGGA 
     4021 AGCGGGACAG CATGGCCCCT GTGTCTCCCA CCGCCAACTC CTGCTTGTTG GAGGAGAACT 
     4081 TTGACTTCAC AAGTCTGGAC TATGAGTCAG AAGGGGATGT TCTTGAAGAT GATGAAGACT 
     4141 ACTTAGCGGA TGAGTTTGGT CAGCCTGTAG TCTCAGACAG TGAGCTTGGA GGAGACGACT 
     4201 CTTCTGATAC CATGAGCGCC TCGACACCTG CCAGCAAGTC TCCCGCACTG GCTAAAAAGA 
     4261 AGCAACATCC AACGTACAAA GCTGGCTTAA TGGTGAAGAA GTCTGCCTCA GATGCGTCTA 
     4321 TCTCCTCCGG AACCCACGGG CAATATTCCA TCTTGCAGAC CGCGAAACTT CTTCCAGGAG 
     4381 CACCTCAGCA ACCGCCCAAG GCTCGAACTG GAATAAGTAA ACCTTACAAC GTCAAACAGA 
     4441 TCAAAACCAC CAATGCTCAG GAGGCGGAAG CAGCTATCCG GTGTCTCCTG GAAGCTGGCG 
     4501 GAGGTGTCCC AGAGTCAGCC CCGGGTGCCA CGCCCCTGAG AAACCAAGGG TCTTCTAAGC 
     4561 CAGAGGCCTC CCTGGGGCCT CCAGTCCTAC CGCGCCGGCC TGTTCCAAGG GTTCCCACTA 
     4621 TGAAGAAACC AACTTTGAGG AGAACAGGAA AGCCCATGTT ATCAGAAGAA CAGTGTGAAC 
     4681 AGCAGCCTGT CCATTTTACA ATGGCTTCCC AGGAAATGAA CCTTGAGACC CCTCCTCCAA 
     4741 TAACAGCTCC CATCCCGCCT GTTCCCAAAC CAAGAACATT TCAGCCTGGG AGAGGTGTTG 
     4801 AGAGGAGGCC GAGTGGTGGC AAGCCAGAAC CGGATGACGC TCCTCCTGTG ACAGGCGCTG 
     4861 TGGAGTTATC GTCTCCAGAG GCCCCAGAGG CCCCGTCCCT GGCTCCCAAG GTGCCTCCTA 
     4921 GGAGGAAGAA GTCTGCACCA GCAGCCTTCC ACCTGCAGGT CCTGCAGAGC AACAGCCAAC 
     4981 TTCTGCAGGG CCTCACATGC TCCAGCAGTT CTCCGTCCCC ACCAAAGCCT GACACCCCCC 
     5041 TGCTCTATCC GCAAATGGCA CTTGGCACTT CATCTGCTAT AAGTCCCGAA ACTGATGGCC 
     5101 CCAGAGTGAC GGAGCCCGAG GCAGCCTCTT TTCATGGCGA TTACCCAGAT CCCTTCTGGA 
     5161 GCCTTCTCCA CCACCCCAAG CTGTTGAATA ATAATACCTG GCTGTCCAAG AGCTCTGAGC 
     5221 CTTTAGACTT GGGGTCCAGG ACCCCTGAGA GGACACACAC AGACTCAGCG CAGGTCAATG 
     5281 CATCAGTGGT TGAGAGGGGG CTTCCACCAG ACCATGGGGG TAAAGACTTC AGTCACTGGA 
     5341 TGGCCGCCAG TAACAAAGAC AAGAGGACAA CATTAGGCGT TACGCGTACG CGGCCGCTCG 
     5401 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5461 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5521 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5581 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5641 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5701 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5761 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5821 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5881 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5941 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6001 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6061 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6121 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6181 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6241 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6301 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6361 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6421 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6481 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6541 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6601 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6661 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6721 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6781 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6841 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6901 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6961 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7021 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7081 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7141 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7201 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7261 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7321 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7381 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7441 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7501 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7561 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7621 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7681 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7741 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7801 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7861 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7921 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7981 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8041 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8101 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8161 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8221 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8281 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8341 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8401 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8461 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8581 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8641 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8701 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8761 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8821 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8881 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8941 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9001 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9061 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9121 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9181 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//