LOCUS       RR200255                9369 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged ORF) - Rat synaptojanin 2 (Synj2), transcript variant 1, (10 ug)
ACCESSION   NM_001113371
VERSION     NM_001113371.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5526..5617 
                     /label="Myc-DDK"  
     misc_feature    5647..6232 
                     /label="PolyA signal"  
     misc_feature    5704..5723 
                     /label="Restriction Enzyme"  
     misc_feature    6508..7180 
                     /label="ColE1"  
     misc_feature    7610..8404 
                     /label="Kan/Neo"  
     misc_feature    8439..8741 
                     /label="SV40 ori"  
     misc_feature    8827..9304 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5508..5514 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5508 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGAGC AAAGGGCTGC GACTGCTGGC GCGGCTGGAC CCCACCGGCC 
     1081 CGAGCAGCGT GCTGCTGGAG GCGCGGGGCC GCGGCGACTG TCTACTCTTT GAGGCCGGCG 
     1141 CGGTGGCCAC GCTGGCTCCG GAAGAGAAGG AAGTCATTAA AGGACTGTAT GGGAAGCCGA 
     1201 CGGATGCCTA TGGCTGCCTC GGGGAGCTGA GTTTAAAATC CGGTGGCGTC CCCTTGAGCT 
     1261 TTCTGGTGTT GGTGACAGGC TGCACGTCAG TGGGCAGAAT TCCAGATGCA GAGATCTACA 
     1321 AAATCACTGG CACTGAGTTT TACCCCCTGC AGGAAGAGGC CAAGGAAGAG GACCGCCTGC 
     1381 CAGCCTTGAA GAAAATCCTG AGTTCAGGGG TGTTCTATTT CGCATGGCCC AATGATGGCG 
     1441 CCTGCTTCGA TCTGACCATC AGGGCTCAGA AACAGGGTGA TGACTGCTCT GAATGGGGGA 
     1501 CCTCCTTCTT CTGGAACCAG CTATTGCATG TGCCTCTGCG GCAGCACCAG GTGAACTGTC 
     1561 ATGACTGGTT GCTGAAAGTC ATCTGTGGGG TGGTGACCAT CCGCACGGTT TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGT CTCATCTCTC GCATCAGCTG TGAACGCGCA GGTGCTCGCT 
     1681 TCCTCACCCG GGGTGTGAAC GACGATGGCC ACGTGTCCAA CTTTGTGGAG ACAGAGCAGG 
     1741 CGATTTACAT GGATGATGGA GTGTCGTCCT TTGTCCAGAT CCGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAGCA GCCAGGGCTC CAGGTTGGCT CCCATCATCT GAGACTGCAC AGAGGCCTAG 
     1861 AGGCCAACGC CCCTGCTTTT GAAAGGCACA TGGTGCTTCT GAAGGAGCAG TACGGGCAGC 
     1921 AGGTGGTGGT GAACCTGCTG GGAAGCAGAG GCGGTGAGGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGTTGCT CTGGGCTTCT TGCCACGCAG GCGACACGCC TATGATAAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTAGG AAGCTAGAGA AATTGGAGAA CCTGTTGAGA CCTCAGTTAA 
     2101 AACTACACTG GGATGACTTT GGCGTATTTG CGAAGGGCGA GAATGTAAGT CCACGGTTTC 
     2161 AGAAAGGCAC TCTGCGGATG AACTGTCTCG ACTGTCTGGA CAGAACCAAC ACTGTACAGT 
     2221 GCTTCATTGC TCTTGAGGTG CTTCATCTGC AGCTTGAGAG CCTAGGGCTG AATTCCAAGC 
     2281 CCATCACTGA CCGCTTCGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGGCATGGCC 
     2341 TGAGCAAGGT GTTCACAGGG AGCAGGGCCC TGGAAGGAAA GGCCAAGGTG GGGAAGCTGA 
     2401 AGGATGGGGC TAGATCCATG TCTCGTACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTACTG CTAGTTGGAG ATGTCTACAA TGAAGAGTCT ACAGACAAAG 
     2521 GCAGGATGCT GCTGGACAAC ACGGCCCTTC TGGGTTTGGG GTCAAATAAA CAAAACAGTC 
     2581 TTTCAGGCAT GCTGGATGGA AAAGCAACAC CCAGGATCCT GAAGGCCATG ACAGAACGCC 
     2641 AGTCAGAATT CACAAATTTC AAGCGGATCC AGATTGCTAT GGGGACCTGG AATGTGAACG 
     2701 GAGGAAAACA ATTCCGTAGC AATCTCCTGG GGACCACTGA ACTCACTGAC TGGCTCCTGG 
     2761 ATGCACCCCA GCTCTCAGGA GCAGTGGACT CTCAGGACGA TGGCGGTCCC GCTGACATAT 
     2821 TTGCCGTCGG GTTTGAGGAG ATGGTGGAGC TGAGTGCAGG AAATATTGTC AATGCCAGCA 
     2881 CCACCAACAG GAAGATGTGG GGTGAGCAGC TCCAGAAAGC CATCTCCCGA TCCCATCGGT 
     2941 ACATCCTCTT GACCTCAGCA CAGCTGGTGG GCGTCTGTCT TTACATCTTT GTACGTCCGT 
     3001 ACCACGTCCC GTTCATCAGG GATGTGGCCA TCGACACCGT GAAGACCGGT ATGGGGGGAA 
     3061 AGGCAGGGAA TAAGGGCGCC GTGGGTATCC GCTTTCAGTT CCACAGCACT AGCTTCTGCT 
     3121 TCATCTGCAG TCACCTGACG GCTGGGCAGT CTCAGGTGAA GGAGAGGAAC GAAGACTACC 
     3181 GGGAGATCAC GCACAAGCTC TCCTTCCCTT CGGGGAGAAA CATATTTTCA CACGATTATG 
     3241 TGTTTTGGTG TGGCGATTTC AACTACCGCA TCGATCTTAC TTACGAAGAA GTCTTCTATT 
     3301 TTGTTAAACG CCAAGACTGG AAGAAACTTA TGGAATTTGA TCAGTTACAG CTACAGAAAT 
     3361 CAAGTGGGAA AATTTTTAAA GACTTTCATG AAGGAACCAT TAACTTCGGA CCCACCTACA 
     3421 AGTATGACGT TGGATCGGCT GCCTATGACA CAAGTGACAA GTGCCGGACC CCAGCCTGGA 
     3481 CAGACAGGGT GCTGTGGTGG AGGAAGAAGC ATCCGTACGA TAAGACAGCT GGCGAACTCA 
     3541 ACCTTCTAGA CAGTGATCTA GACGGTGATG CCAACATCAG ACATACCTGG TCTCCGGGCA 
     3601 CTCTCAAGTA CTACGGCCGT GCCGAGCTGC AGGCATCTGA TCACAGACCT GTGCTGGCCA 
     3661 TCGTAGAGGT GGAGGTTCAA GAGGTCGATG TGGGAGCCCG GGAGAGAGTC TTCCAGGAAG 
     3721 TGTCCTCTGT CCAAGGCCCG CTGGACGCCA CTGTTATTGT AAACCTTCAG TCTCCAACCC 
     3781 TTGAAGAGAG AAATGAATTT CCAGAGGACC TGCGCACAGA ACTTATGCAG ACTTTGGGGA 
     3841 ATTATGGGAC GATTATTCTG GTCAGGATCA ACCAAGGGCA GATGCTGGTG ACATTTGCAG 
     3901 ACAGCCACTC AGCTCTCAGT GTGCTGGATG TGGATGGTAT GAAGGTGAAA GGCAGGGCCG 
     3961 TGAAGATTCG ACCAAAGACC AAAGATTGGC TAGAAGGCTT GAGAGAGGAG CTCATTCGGA 
     4021 AGCGGGACAG CATGGCCCCT GTGTCTCCCA CCGCCAACTC CTGCTTGTTG GAGGAGAACT 
     4081 TTGACTTCAC AAGTCTGGAC TATGAGTCAG AAGGGGATGT TCTTGAAGAT GATGAAGACT 
     4141 ACTTAGCGGA TGAGTTTGGT CAGCCTGTAG TCTCAGACAG TGAGCTTGGA GGAGACGACT 
     4201 CTTCTGATAC CATGAGCGCC TCGACACCTG CCAGCAAGTC TCCCGCACTG GCTAAAAAGA 
     4261 AGCAACATCC AACGTACAAA GACGACGCTG ACCTGATGAC GTTAAAGCTG GAGCTGGAAG 
     4321 TTGCTGGGAA TTTTCGCCAC CGTTCTCCGA GCAGGTCCCT GTCTGTCCCC AATAGGCCTC 
     4381 GGCCACCTCA CCCTCCACAA AGACCCCCCC CTCCAACTGG CTTAATGGTG AAGAAGTCTG 
     4441 CCTCAGATGC GTCTATCTCC TCCGGAACCC ACGGGCAATA TTCCATCTTG CAGACCGCGA 
     4501 AACTTCTTCC AGGAGCACCT CAGCAACCGC CCAAGGCTCG AACTGGAATA AGTAAACCTT 
     4561 ACAACGTCAA ACAGATCAAA ACCACCAATG CTCAGGAGGC GGAAGCAGCT ATCCGGTGTC 
     4621 TCCTGGAAGC TGGCGGAGGT GTCCCAGAGT CAGCCCCGGG TGCCACGCCC CTGAGAAACC 
     4681 AAGGGTCTTC TAAGCCAGAG GCCTCCCTGG GGCCTCCAGT CCTACCGCGC CGGCCTGTTC 
     4741 CAAGGGTTCC CACTATGAAG AAACCAACTT TGAGGAGAAC AGGAAAGCCC ATGTTATCAG 
     4801 AAGAACAGTG TGAACAGCAG CCTGTCCATT TTACAATGGC TTCCCAGGAA ATGAACCTTG 
     4861 AGACCCCTCC TCCAATAACA GCTCCCATCC CGCCTGTTCC CAAACCAAGA ACATTTCAGC 
     4921 CTGGGAGAGG TGTTGAGAGG AGGCCGAGTG GTGGCAAGCC AGAACCGGAT GACGCTCCTC 
     4981 CTGTGACAGG CGCTGTGGAG TTATCGTCTC CAGAGGCCCC AGAGGCCCCG TCCCTGGCTC 
     5041 CCAAGGTGCC TCCTAGGAGG AAGAAGTCTG CACCAGCAGC CTTCCACCTG CAGGTCCTGC 
     5101 AGAGCAACAG CCAACTTCTG CAGGGCCTCA CATGCTCCAG CAGTTCTCCG TCCCCACCAA 
     5161 AGCCTGACAC CCCCCTGCTC TATCCGCAAA TGGCACTTGG CACTTCATCT GCTATAAGTC 
     5221 CCGAAACTGA TGGCCCCAGA GTGACGGAGC CCGAGGCAGC CTCTTTTCAT GGCGATTACC 
     5281 CAGATCCCTT CTGGAGCCTT CTCCACCACC CCAAGCTGTT GAATAATAAT ACCTGGCTGT 
     5341 CCAAGAGCTC TGAGCCTTTA GACTTGGGGT CCAGGACCCC TGAGAGGACA CACACAGACT 
     5401 CAGCGCAGGT CAATGCATCA GTGGTTGAGA GGGGGCTTCC ACCAGACCAT GGGGGTAAAG 
     5461 ACTTCAGTCA CTGGATGGCC GCCAGTAACA AAGACAAGAG GACAACATTA GGCGTTACGC 
     5521 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5581 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5641 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5701 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5761 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5821 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5881 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5941 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     6001 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6061 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6121 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6181 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6241 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6301 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6361 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6421 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6481 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6541 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6601 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6661 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6721 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6781 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6841 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6901 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6961 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7021 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7081 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7141 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7201 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7261 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7321 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7381 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7441 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7501 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7561 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7621 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7681 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7741 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7801 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7861 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7921 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7981 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     8041 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8101 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8161 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8221 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8281 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8341 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8401 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8461 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8521 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8581 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8641 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8701 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8761 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8821 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8881 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8941 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     9001 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9061 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9121 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9181 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9241 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9301 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9361 GCGAATTTT
//