LOCUS       RR200252                7821 bp    DNA     circular  SYN 
DEFINITION  Trpc4 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily C, member 4 (Trpc4), transcript variant alpha, (10 ug)
ACCESSION   NM_080396
VERSION     NM_080396.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3976..4067 
                     /label="Myc-DDK"  
     misc_feature    4097..4682 
                     /label="PolyA signal"  
     misc_feature    4154..4173 
                     /label="Restriction Enzyme"  
     misc_feature    4958..5630 
                     /label="ColE1"  
     misc_feature    6060..6854 
                     /label="Kan/Neo"  
     misc_feature    6889..7191 
                     /label="SV40 ori"  
     misc_feature    7277..7754 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3951..3958 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3951 
                     /label="Trpc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAGTTC TATTACAAAC GAAATGTCAA CGCCCCCTAC CGAGACCGCA 
     1081 TCCCACTGAG GATCGTCAGG GCAGAATCTG AACTCTCACC ATCAGAGAAA GCCTACTTGA 
     1141 ATGCCGTGGA AAAGGGGGAC TATGCAAGCG TCAAGAAATC TCTGGAGGAA GCCGAGATTT 
     1201 ATTTTAAAAT CAACATTAAC TGCATTGACC CCCTTGGGAG GACTGCTCTT CTCATTGCCA 
     1261 TTGAAAATGA GAACCTGGAG CTGATTGAAC TGTTGTTGAG TTTCAATGTC TATGTTGGCG 
     1321 ATGCGCTACT TCACGCCATC AGGAAAGAGG TGGTTGGAGC CGTGGAGCTA CTGCTGAACC 
     1381 ACAAAAAGCC CAGCGGAGAG AAGCAGGTGC CTCCCATCCT CCTTGACAAA CAGTTCTCTG 
     1441 AATTCACCCC AGACATCACG CCTATCATCT TGGCTGCACA TACAAATAAT TATGAGATAA 
     1501 TCAAACTCTT GGTCCAGAAG GGTGTCTCGG TGCCCAGACC CCACGAGGTC CGCTGTAACT 
     1561 GTGTTGAGTG TGTCTCCAGC TCAGACGTGG ACAGCCTCAG GCACTCACGG TCCAGGCTCA 
     1621 ACATCTACAA GGCTTTGGCC AGCCCCTCGC TCATTGCGCT GTCAAGTGAA GACCCTTTCC 
     1681 TCACCGCCTT TCAGTTAAGC TGGGAGCTGC AAGAACTGAG TAAGGTGGAG AATGAATTCA 
     1741 AGTCGGAGTA TGAGGAGCTG TCTAGACAGT GCAAACAGTT TGCTAAGGAC CTCCTAGATC 
     1801 AGACACGGAG TTCCAGAGAG CTGGAAATCA TTCTTAATTA CCGTGATGAC AATAGCCTGA 
     1861 TCGAAGAACA GAGTGGAAAT GATCTTGCGA GGCTAAAATT AGCCATTAAG TACCGTCAAA 
     1921 AAGAGTTTGT TGCTCAGCCC AACTGCCAGC AGCTGCTCGC TTCCCGCTGG TACGATGAGT 
     1981 TCCCAGGCTG GAGGAGAAGA CACTGGGCGG TGAAGATGGT GACATGTTTC ATAATAGGAC 
     2041 TACTCTTCCC CGTCTTCTCC GTGTGCTACC TGATAGCTCC CAAAAGCCCA CTTGGACTGT 
     2101 TCATCAGAAA GCCATTTATC AAGTTTATCT GCCACACAGC CTCCTATCTG ACCTTTTTGT 
     2161 TTCTGCTGCT GCTAGCCTCT CAGCACATCG ACAGGTCAGA CTTGAACAGG CAAGGTCCAC 
     2221 CACCAACCAT CGTGGAGTGG ATGATATTAC CGTGGGTCCT GGGTTTTATA TGGGGAGAGA 
     2281 TTAAACAGAT GTGGGATGGC GGACTTCAGG ATTACATCCA CGACTGGTGG AATCTAATGG 
     2341 ACTTTGTGAT GAACTCCTTG TATCTGGCGA CAATCTCCTT GAAGATTGTC GCATTTGTAA 
     2401 AGTACAGTGC TCTGAACCCA CGGGAATCAT GGGACATGTG GCACCCCACC CTGGTGGCAG 
     2461 AGGCTTTATT CGCAATTGCA AACATCTTCA GTTCCCTCCG CCTGATCTCT CTGTTCACTG 
     2521 CCAATTCTCA CCTGGGGCCT CTGCAGATAT CTCTGGGAAG AATGCTCCTG GACATCCTAA 
     2581 AGTTCTTATT CATATACTGC CTCGTGCTGC TAGCTTTTGC AAATGGCCTA AATCAACTGT 
     2641 ACTTCTACTA TGAAGAAACG AAGGGGTTAA GCTGCAAAGG CATACGGTGC GAAAAACAGA 
     2701 ACAACGCGTT CTCCACGTTA TTTGAGACTC TACAGTCCCT GTTTTGGTCA ATATTTGGAC 
     2761 TCATCAATCT CTATGTTACC AATGTCAAAG CCCAGCATGA GTTCACTGAA TTTGTTGGGG 
     2821 CCACCATGTT TGGCACATAT AACGTCATCT CTCTGGTTGT CCTCCTGAAC ATGCTGATCG 
     2881 CTATGATGAA TAATTCTTAC CAACTAATTG CCGACCACGC AGATATAGAG TGGAAATTTG 
     2941 CTCGAACAAA GCTTTGGATG AGCTACTTTG AAGAAGGGGG TACCCTGCCT ACACCTTTCA 
     3001 ATGTCATCCC AAGCCCCAAG TCCCTGTGGT ACCTGGTCAA GTGGATATGG ACGCACTTAT 
     3061 GTAAGAAAAA GATGAGAAGA AAGCCAGAAA GCTTTGGGAC AATTGGGCGG CGTGCTGCTG 
     3121 ATAACTTGAG AAGGCATCAC CAATACCAAG AGGTGATGAG GAATCTGGTG AAGCGGTACG 
     3181 TGGCAGCCAT GATCAGAGAG GCAAAAACTG AAGAAGGCTT GACAGAGGAG AATGTTAAGG 
     3241 AACTAAAGCA AGACATTTCT AGCTTCCGCT TCGAAGTTCT GGGATTGCTC CGGGGAAGCA 
     3301 AGCTCTCAAC AATACAGTCA GCCAACGCAG CGAGTTCAGC CAGCTCCGCG GACTCCGATG 
     3361 AGAAGAGCCA CAGCGAAGGT AATGGCAAGG ACAAGAGAAA GAATCTCAGC CTCTTTGATT 
     3421 TAACCACTCT GATCCACCCG CGGTCGGCAG TCATTGCCTC CGAGAGACAT AACCTAAGCA 
     3481 ATGGTTCTGC CCTGGTGGTG CAGGAGCCGC CCAGGGAGAA GCAGAGGAAA GTGAATTTTG 
     3541 TGGCTGATAT CAAAAACTTC GGGTTATTTC ATAGACGGTC AAAGCAAAAT GCTGCTGAGC 
     3601 AAAACGCAAA CCAAATCTTC TCTGTTTCAG AAGAAATTAC TCGTCAACAG GCGGCAGGAG 
     3661 CACTTGAGAG AAATATCCAA CTGGAATCCC AAGGATTAGC TTCACGGGGT GACCGCAGCA 
     3721 TTCCTGGTCT CAATGAACAG TGTGTGCTAG TAGACCATAG AGAAAGGAAT ACGGACACTT 
     3781 TGGGTTTACA GGTAGGCAAG AGAGTGTGCT CCTCCTTCAA GTCGGAGAAG GTGGTGGTGG 
     3841 AAGACACCGT CCCTATTATA CCAAAGGAGA AACACGCCCA GGAGGAGGAC TCAAGCATAG 
     3901 ATTATGATTT AAGCCCCACG GACACAGTTG CCCATGAAGA TTATGTGACC ACGAGATTGA 
     3961 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4021 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4081 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4141 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4201 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4261 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4321 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4381 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4441 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4501 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4561 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4621 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4681 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4741 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4801 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4861 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4921 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4981 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5041 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5101 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5161 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5221 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5281 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5341 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5401 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5461 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5521 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5581 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5641 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5701 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5761 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5821 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5881 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5941 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6001 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6061 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6121 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6181 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6241 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6301 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6361 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6421 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6481 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6541 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6601 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6661 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6721 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6781 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6841 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6901 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6961 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7021 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7081 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7141 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7261 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7321 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7381 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7801 CAAAAATTTA ACGCGAATTT T
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