LOCUS       RR200193                8637 bp    DNA     circular  SYN 
DEFINITION  Shank2 (Myc-DDK-tagged ORF) - Rat SH3 and multiple ankyrin repeat domains 2 (Shank2), transcript variant 2, (10 ug)
ACCESSION   NM_001004133
VERSION     NM_001004133.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4794..4885 
                     /label="Myc-DDK"  
     misc_feature    4915..5500 
                     /label="PolyA signal"  
     misc_feature    4972..4991 
                     /label="Restriction Enzyme"  
     misc_feature    5776..6448 
                     /label="ColE1"  
     misc_feature    6878..7672 
                     /label="Kan/Neo"  
     misc_feature    7707..8009 
                     /label="SV40 ori"  
     misc_feature    8095..8572 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4776..4782 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4776 
                     /label="Shank2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCGTC CCCGGCGGTG GAGCAGCCAC CGTGATGATG ACCGGTTACA 
     1081 ATAATGGTCG CTATCCCCGG AATTCTCTCT ACAGTGACTG CATTATTGAG GACAAGACGG 
     1141 TGGTCCTGCA GAAGAAAGAC AACGAGGGCT TTGGATTTGT GCTCCGAGGG GCAAAAGCTG 
     1201 ATACCCCCAT TGAGGAATTC ACACCCACGC CGGCATTTCC AGCCCTGCAG TACCTGGAGT 
     1261 CCGTGGATGA AGGAGGGGTG GCATGGCAAG CCGGACTAAG GACCGGGGAC TTCTTGATTG 
     1321 AGGTTAACAA TGAAAATGTC GTCAAGGTGG GCCACAGGCA GGTGGTGAAC ATGATCCGCC 
     1381 AGGGAGGGAA TCACCTCGTC CTTAAGGTCG TCACGGTGAC CAGGAATCTA GACCCTGATG 
     1441 ATACAGCCAG AAAGAAAGCT CCCCCACCTC CAAAGCGGGC TCCGACCACG GCCCTCACCG 
     1501 TGCGTTCCAA GTCCATGACA GCGGAATTGG AGGAACTCGT GGATAAAGAT AAACCGGAAG 
     1561 AGATAGTCCC AGCCTCCAAG CCCTCCAGGA CTGCAGAGAA CGTGGCCATA GAATCCAGGG 
     1621 TGGCAACCAT CAAGCAGCGG CCCACAAGCC GGTGCTTTCC AGCTGCCTCT GATGTGAACT 
     1681 CCGTGTACGA GCGCCAAGGG ATTGCTGTAA TGACGCCCAC GGTCCCTGGG AGCCCGAAAG 
     1741 GCCCATTTCT GGGCCTCCCT CGAGGTACGA TGCGAAGGCA GAAATCGATA GACAGCAGAA 
     1801 TCTTTCTATC AGGGATAACA GAGGAAGAGC GGCAGTTTCT GGCTCCCCCA ATGCTGAAGT 
     1861 TCACCCGAAG CTTGTCCATG CCAGACACTT CTGAGGACAT CCCCCCTCCG CCACAGTCTG 
     1921 TGCCCCCCTC TCCCCCTCCA CCTTCCCCCA CCACATACAA CTGTCCCAGG TCCCCGACTC 
     1981 CAAGAGTCTA TGGGACAATT AAGCCTGCGT TCAACCAGAA CCCCGTCGCC AAGGTGCCCC 
     2041 CAGCCACCAG GTCTGACACT GTGGCCACCA TGATGCGGGA AAAGGGGATG TTCTACAGGA 
     2101 GAGAGCTGGA CCGCTTTTCC CTGGACTCAG AAGACGTCTA CAGCCGCAGC CCCGCCCCAC 
     2161 AGGCTGCCTT CCGCACCAAG CGGGGACAGA TGCCTGAGAA CCCGTACTCG GAGGTGGGAA 
     2221 AGATAGCCAG CAAGGCCGTC TATGTCCCTG CCAAGCCAGC CAGGCGGAAG GGCATGCTGG 
     2281 TAAAGCAGTC CAACGTGGAG GACAGCCCTG AGAAGACGTG CTCCATCCCC ATCCCAACCA 
     2341 TCATAGTCAA GGAACCCTCC ACCAGCAGCA GCGGCAAGAG CAGCCAGGGG AGCAGCATGG 
     2401 AGATCGACCC CCAGGCCACT GAGCCCGGCC AGCTGCGGCC AGATGACAGC CTCACCGTCA 
     2461 GCAGCCCTTT CGCGGCAGCC ATCGCTGGGG CCGTGCGTGA CCGGGAGAAG CGTCTGGAAG 
     2521 CCAGGAGGAA TTCCCCGGCC TTCCTCTCCA CCGACCTGGG AGATGAGGAC GTGGGTCTGG 
     2581 GGCCTCCTGC TCCCCGGATG CAGCCCTCCA AGTTTCCCGA GGAGGGTGGG TTTGGTGATG 
     2641 AGGATGAAAC GGAACAGCCG CTATTGCCTA CCCCTGGAGC AGCGCCCAGG GAGCTGGAGA 
     2701 ACCACTTCCT AGGTGGTGGT GAGGCTGGTG CTCAGGGGGA GGCTGGGGGA CCCCTGAGTT 
     2761 CCACATCCAA AGCCAAGGGG CCTGAAAGTG GCCCAGCAGC CGCCCTCAAG AGCAGCAGCC 
     2821 CAGCCAGCCC TGAGAATTAC GTGCACCCAC TCACGGGGCG GCTGCTCGAC CCCAGCTCCC 
     2881 CGCTGGCCCT GGCACTGTCC GCCAGGGACC GAGCCATGCA GGAGTCCCAG CAAGGACACA 
     2941 AGGGGGAGGC CCCCAAGGCC GACCTTAACA AGCCTCTTTA CATCGATACC AAAATGCGGC 
     3001 CCAGTGTGGA GTCCGGCTTT CCACCGGTCA CCAGACAGAA CACCAGGGGT CCCCTGCGAA 
     3061 GGCAGGAGAC AGAGAACAAG TATGAGACGG ACCTGAGCAA GGACCGGAGG GCTGACGACA 
     3121 AGAAGAACAT GCTGATCAAC ATCGTGGACA CTGCCCAGCA GAAGTCAGCC GGCCTACTGA 
     3181 TGGTGCACAC AGTGGACATT CCCGTGGCGG GGCCACCCCT GGAGGAAGAG GAGGACAGAG 
     3241 AGGATGGGGA TACAAAGCCA GACCACTCAC CCTCCACAGT GCCAGAAGGC GTTCCCAAAA 
     3301 CCGAAGGTGC TTTACAGATC TCCGCTGCCC CGGAGCCCGC CGCTGCGCCC GGCAGGACCA 
     3361 TCGTGGCAGC GGGCTCCGTG GAAGAGGCGG TAATTCTGCC ATTCCGCATC CCCCCTCCCC 
     3421 CTCTGGCGTC CGTGGACTTG GATGAGGACT TTCTTTTCAC AGAACCATTG CCTCCTCCCC 
     3481 TGGAATTCGC CAATAGTTTT GATATCCCCG ACGACCGGGC AGCTTCAGTT CCCGCTCTGG 
     3541 CTGACCTGGT CAAGCAGAAG AAAAGTGACA CCCCTCAGCC CCCTTCGTTG AACTCCAGCC 
     3601 AACCAGCCAA CTCTACAGAC AGTAAGAAGC CAGCCGGCAT CTCAAACTGT CTGCCCTCCT 
     3661 CATTCCTGCC ACCCCCCGAA AGCTTTGACG CGGTCACCGA CTCGGGGATC GAGGAGGTGG 
     3721 ACAGCCGGAG TAGCAGCGAC CACCACCTGG AGACAACCAG CACCATCTCC ACAGTGTCCA 
     3781 GCATCTCCAC GCTGTCTTCA GAGGGCGGGG AGAGCATGGA TACTTGCACA GTCTATGCAG 
     3841 ACGGGCAAGC CTTTGTGGTT GACAAGCCCC CAGTACCTCC AAAGCCAAAA ATGAAGCCCA 
     3901 TCGTTCACAA AAGCAACGCA CTTTACCAAG ACACGCTCCC AGAAGAGGAC ACAGATGGCT 
     3961 TTGTGATCCC CCCACCTGCA CCCCCGCCCC CGCCCGGCAG TGCCCAGGCC GGTGTGGCGA 
     4021 AGGTCATCCA GCCAAGGACC TCCAAGTTGT GGGGTGATGT TACAGAGGTC AAAAGCCCAA 
     4081 TTCTCTCAGG CCCAAAGGCA AATGTCATTA GTGAGTTAAA CTCCATTCTG CAGCAGATGA 
     4141 ACAGGGGGAA ATCGGTCAAG CCTGGGGAAG GGCTGGAGCT GCCCGTGGGA GCCAAGTCAG 
     4201 CTAACCTCGC TCCAAGAAGC CCGGAGGTCA TGAGCACTGT CTCAGGTACA CGGAGCACGA 
     4261 CGGTCACCTT CACTGTCCGC CCCGGAACCT CCCAGCCCAT CACCCTACAG AGCCGGCCCC 
     4321 CTGACTATGA AAGCAGGACC TCAGGACCGA GACGTGCCCC AAGCCCTGTG GTTTCGCCAA 
     4381 CGGAATTGAG CAAAGAGATT CTGCCCACCC CTCCGTCTGC TGCAGCAGCC TCTCCCTCCC 
     4441 CCACACTCTC AGATGTCTTT AGCCTTCCAA GCCAGTCCCC TGCAGGGGAC CTCTTTGGCT 
     4501 TGAACCCAGC AGGACGCAGC AGGTCACCAT CTCCTTCAAT ATTGCAACAG CCAATCTCAA 
     4561 ATAAGCCTTT TACAACTAAG CCTGTCCACC TGTGGACGAA ACCAGATGTG GCAGACTGGC 
     4621 TGGAAAGTCT GAACTTGGGT GAACACAAGG AGACGTTCAT GGACAATGAG ATTGACGGCA 
     4681 GCCATCTGCC AAACCTCCAG AAGGAAGACT TGATAGATCT TGGGGTGACT CGAGTTGGGC 
     4741 ACAGGATGAA CATAGAAAGG GCTTTGAAAC AGCTGCTGGA CAGAACGCGT ACGCGGCCGC 
     4801 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4861 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4921 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4981 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5041 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5101 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5161 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5221 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5281 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5341 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5401 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5461 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5521 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5581 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5641 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5701 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5761 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5821 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5881 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5941 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6001 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6061 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6121 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6181 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6241 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6301 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6361 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6421 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6481 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6541 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6601 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6661 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6721 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6781 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6841 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6901 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6961 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7021 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7081 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7141 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7201 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7261 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7321 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7381 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7441 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7501 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7561 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7621 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7681 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7741 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7801 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7861 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8041 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8101 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8161 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8221 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8281 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8341 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8401 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8461 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8521 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8581 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//