LOCUS       RR200192                9291 bp    DNA     circular  SYN 
DEFINITION  Shank2 (Myc-DDK-tagged ORF) - Rat SH3 and multiple ankyrin repeat domains 2 (Shank2), transcript variant 4, (10 ug)
ACCESSION   NM_133441
VERSION     NM_133441.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5448..5539 
                     /label="Myc-DDK"  
     misc_feature    5569..6154 
                     /label="PolyA signal"  
     misc_feature    5626..5645 
                     /label="Restriction Enzyme"  
     misc_feature    6430..7102 
                     /label="ColE1"  
     misc_feature    7532..8326 
                     /label="Kan/Neo"  
     misc_feature    8361..8663 
                     /label="SV40 ori"  
     misc_feature    8749..9226 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5430..5436 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5430 
                     /label="Shank2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTTTG TTAAATGCCT TCACCAAGAA GGAAGTGCCC TTCCGAGAGG 
     1081 CCCCAGCTTA TTCCAACCGC AGGCGGCGGC CCCCCAACAC ATTGGCTGCC CCCAGAGTTC 
     1141 TTCTGCGTTC CAATAGCGAC AACAACCTCA ATGCCGGTGC GCCTGAATGG GCTGTCTGCT 
     1201 CTGCAGCCAC CTCCCACCGA AGCCTCTCAC CCCAGCTGCT GCAGCAGACA CCCAGCAAAC 
     1261 CTGATGGAGC CACAAAGAGC CTTGGAAGCT ACGCCCCTGG GCCCCGCAGC CGCTCTCCCT 
     1321 CACTCAACAG GCTGGGTGGC GCTGGTGAGG ATGGCAAGAG GCCACAGCCG CCACATTGGC 
     1381 ATGTGGGGTC GCCCTTCACT CCTGGTGCCA ACAAGGACTC CCTCTCGACC TTTGAGTACC 
     1441 CAGGGCCCCG GAGGAAGCTG TATAGTGCGG TGCCTGGGAG ACTCTTCGTC GCCATCAAGC 
     1501 CATACCAACC CCAAGTCGAC GGCGAGATCC CCCTTCACCG AGGCGACAGG GTCAAAGTTC 
     1561 TGAGCATCGG CGAGGGCGGC TTCTGGGAAG GCAGCGCCCG TGGCCACATC GGGTGGTTCC 
     1621 CAGCTGAGTG TGTGGAGGAG GTACAATGTA AGCCCCGGGA CAGCCAAGCA GAAACCCGTG 
     1681 CGGACCGCAG CAAGAAGCTC TTTCGGCATT ACACAGTGGG CTCCTATGAC AGCTTTGATG 
     1741 CTGCCAGTGA CTGCATTATT GAGGACAAGA CGGTGGTCCT GCAGAAGAAA GACAACGAGG 
     1801 GCTTTGGATT TGTGCTCCGA GGGGCAAAAG CTGATACCCC CATTGAGGAA TTCACACCCA 
     1861 CGCCGGCATT TCCAGCCCTG CAGTACCTGG AGTCCGTGGA TGAAGGAGGG GTGGCATGGC 
     1921 AAGCCGGACT AAGGACCGGG GACTTCTTGA TTGAGGTTAA CAATGAAAAT GTCGTCAAGG 
     1981 TGGGCCACAG GCAGGTGGTG AACATGATCC GCCAGGGAGG GAATCACCTC GTCCTTAAGG 
     2041 TCGTCACGGT GACCAGGAAT CTAGACCCTG ATGATACAGC CAGAAAGAAA GCTCCCCCAC 
     2101 CTCCAAAGCG GGCTCCGACC ACGGCCCTCA CCCTGCGTTC CAAGTCCATG ACAGCGGAAT 
     2161 TGGAGGAACT CGTGGATAAA GCCTCAGTCC GGAAGAAGAA GGATAAACCG GAAGAGATAG 
     2221 TCCCAGCCTC CAAGCCCTCC AGGACTGCAG AGAACGTGGC CATAGAATCC AGGGTGGCAA 
     2281 CCATCAAGCA GCGGCCCACA AGCCGGTGCT TTCCAGCTGC CTCTGATGTG AACTCCGTGT 
     2341 ACGAGCGCCA AGGGATTGCT GTAATGACGC CCACGGTCCC TGGGAGCCCG AAAGGCCCAT 
     2401 TTCTGGGCCT CCCTCGAGGT ACGATGCGAA GGCAGAAATC GATAGACAGC AGAATCTTTC 
     2461 TATCAGGGAT AACAGAGGAA GAGCGGCAGT TTCTGGCTCC CCCAATGCTG AAGTTCACCC 
     2521 GAAGCTTGTC CATGCCAGAC ACTTCTGAGG ACATCCCCCC TCCGCCACAG TCTGTGCCCC 
     2581 CCTCTCCCCC TCCACCTTCC CCCACCACAT ACAACTGTCC CAGGTCCCCG ACTCCAAGAG 
     2641 TCTATGGGAC AATTAAGCCT GCGTTCAACC AGAACCCCGT CGCCAAGGTG CCCCCAGCCA 
     2701 CCAGGTCTGA CACTGTGGCC ACCATGATGC GGGAAAAGGG GATGTTCTAC AGGAGAGAGC 
     2761 TGGACCGCTT TTCCCTGGAC TCAGAAGACG TCTACAGCCG CAGCCCCGCC CCACAGGCTG 
     2821 CCTTCCGCAC CAAGCGGGGA CAGATGCCTG AGAACCCGTA CTCGGAGGTG GGAAAGATAG 
     2881 CCAGCAAGGC CGTCTATGTC CCTGCCAAGC CAGCCAGGCG GAAGGGCATG CTGGTAAAGC 
     2941 AGTCCAACGT GGAGGACAGC CCTGAGAAGA CGTGCTCCAT CCCCATCCCA ACCATCATAG 
     3001 TCAAGGAACC CTCCACCAGC AGCAGCGGCA AGAGCAGCCA GGGGAGCAGC ATGGAGATCG 
     3061 ACCCCCAGGC CACTGAGCCC GGCCAGCTGC GGCCAGATGA CAGCCTCACC GTCAGCAGCC 
     3121 CCTTCGCGGC AGCCATCGCT GGGGCCGTGC GTGACCGGGA GAAGCGTCTG GAAGCCAGGA 
     3181 GGAATTCCCC GGCCTTCCTC TCCACCGACC TGGGAGATGA GGACGTGGGT CTGGGGCCTC 
     3241 CTGCTCCCCG GATGCAGCCC TCCAAGTTTC CCGAGGAGGG TGGGTTTGGT GATGAGGATG 
     3301 AAACGGAACA GCCGCTATTG CCTACCCCTG GAGCAGCGCC CAGGGAGCTG GAGAACCACT 
     3361 TCCTAGGTGG TGGTGAGGCT GGTGCTCAGG GGGAGGCTGG GGGACCCCTG AGTTCCACAT 
     3421 CCAAAGCCAA GGGGCCTGAA AGTGGCCCAG CAGCCGCCCT CAAGAGCAGC AGCCCAGCCA 
     3481 GCCCTGAGAA TTACGTGCAC CCACTCACGG GGCGGCTGCT CGACCCCAGC TCCCCGCTGG 
     3541 CCCTGGCACT GTCCGCCAGG GACCGAGCCA TGCAGGAGTC CCAGCAAGGA CACAAGGGGG 
     3601 AGGCCCCCAA GGCCGACCTT AACAAGCCTC TCTACATCGA TACCAAAATG CGGCCCAGTG 
     3661 TGGAGTCCGG CTTTCCACCG GTCACCAGAC AGAACACCAG GGGTCCCCTG CGAAGGCAGG 
     3721 AGACAGAGAA CAAGTATGAG ACGGACCTGA GCAAGGACCG GAGGGCTGAC GACAAGAAGA 
     3781 ACATGCTGAT CAACATCGTG GACACTGCCC AGCAGAAGTC AGCCGGCCTA CTGATGGTGC 
     3841 ACACAGTGGA CATTCCCGTG GCGGGGCCAC CCCTGGAGGA AGAGGAGGAC AGAGAGGATG 
     3901 GGGATACAAA GCCAGACCAC TCACCCTCCA CAGTGCCAGA AGGCGTTCCC AAAACCGAAG 
     3961 GTGCTTTACA GATCTCCGCT GCCCCGGAGC CCGCCGCTGC GCCCGGCAGG ACCATCGTGG 
     4021 CAGCGGGCTC CGTGGAAGAG GCGGTAATTC TGCCATTCCG CATCCCCCCT CCCCCTCTGG 
     4081 CGTCCGTGGA CTTGGATGAG GACTTTCTTT TCACAGAACC ATTGCCTCCT CCCCTGGAAT 
     4141 TCGCCAATAG TTTTGATATC CCCGACGACC GGGCAGCTTC AGTTCCCGCT CTGGCTGACC 
     4201 TGGTCAAGCA GAAGAAAAGT GACACCCCTC AGCCCCCTTC GTTGAACTCC AGCCAACCAG 
     4261 CCAACTCTAC AGACAGTAAG AAGCCAGCCG GCATCTCAAA CTGTCTGCCC TCCTCATTCC 
     4321 TGCCACCCCC CGAAAGCTTT GACGCGGTCA CCGACTCGGG GATCGAGGAG GTGGACAGCC 
     4381 GGAGTAGCAG CGACCACCAC CTGGAGACAA CCAGCACCAT CTCCACAGTG TCCAGCATCT 
     4441 CCACGCTGTC TTCAGAGGGC GGGGAGAGCA TGGATACTTG CACAGTCTAT GCAGACGGGC 
     4501 AAGCCTTTGT GGTTGACAAG CCCCCAGTAC CTCCAAAGCC AAAAATGAAG CCCATCGTTC 
     4561 ACAAAAGCAA CGCACTTTAC CAAGACACGC TCCCAGAAGA GGACACAGAT GGCTTTGTGA 
     4621 TCCCCCCACC TGCACCCCCG CCCCCGCCCG GCAGTGCCCA GGCCGGTGTG GCGAAGGTCA 
     4681 TCCAGCCAAG GACCTCCAAG TTGTGGGGTG ATGTTACAGA GGTCAAAAGC CCAATTCTCT 
     4741 CAGGCCCAAA GGCAAATGTC ATTAGTGAGT TAAACTCCAT TCTGCAGCAG ATGAACAGGG 
     4801 GGAAATCGGT CAAGCCTGGG GAAGGGCTGG AGCTGCCCGT GGGAGCCAAG TCAGCTAACC 
     4861 TCGCTCCAAG AAGCCCGGAG GTCATGAGCA CTGTCTCAGG TACACGGAGC ACGACGGTCA 
     4921 CCTTCACTGT CCGCCCCGGA ACCTCCCAGC CCATCACCCT ACAGAGCCGG CCCCCTGACT 
     4981 ATGAAAGCAG GACCTCAGGA CCGAGACGTG CCCCAAGCCC TGTGGTTTCG CCAACGGAAT 
     5041 TGAGCAAAGA GATTCTGCCC ACCCCTCCGT CTGCTGCAGC AGCCTCTCCC TCCCCCACAC 
     5101 TCTCAGATGT CTTTAGCCTT CCAAGCCAGT CCCCTGCAGG GGACCTCTTT GGCTTGAACC 
     5161 CAGCAGGACG CAGCAGGTCA CCATCTCCTT CAATATTGCA ACAGCCAATC TCAAATAAGC 
     5221 CTTTTACAAC TAAGCCTGTC CACCTGTGGA CGAAACCAGA TGTGGCAGAC TGGCTGGAAA 
     5281 GTCTGAACTT GGGTGAACAC AAGGAGACGT TCATGGACAA TGAGATTGAC GGCAGCCATC 
     5341 TGCCAAACCT CCAGAAGGAA GACTTGATAG ATCTTGGGGT GACTCGAGTT GGGCACAGGA 
     5401 TGAACATAGA AAGGGCTTTG AAACAGCTGC TGGACAGAAC GCGTACGCGG CCGCTCGAGC 
     5461 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5521 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5581 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5641 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5701 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5761 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5821 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5881 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5941 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6001 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6061 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6121 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6181 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6241 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6301 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6361 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6421 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6481 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6541 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6601 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6661 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6721 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6781 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6841 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6901 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6961 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7021 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7081 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7141 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7201 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7261 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7321 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7381 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7441 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7501 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7561 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7621 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7681 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7741 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7801 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7861 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7921 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7981 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8041 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8101 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8161 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8221 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8281 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8341 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8401 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8461 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8521 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8641 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8701 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8761 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8821 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8881 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8941 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9001 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9061 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9121 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9181 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9241 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//