LOCUS       RR200191                8658 bp    DNA     circular  SYN 
DEFINITION  Shank2 (Myc-DDK-tagged ORF) - Rat SH3 and multiple ankyrin repeat domains 2 (Shank2), transcript variant 3, (10 ug)
ACCESSION   NM_133440
VERSION     NM_133440.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="Shank2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCGTC CCCGGCGGTG GAGCAGCCAC CGTGATGATG ACCGGTTACA 
     1081 ATAATGGTCG CTATCCCCGG AATTCTCTCT ACAGTGACTG CATTATTGAG GACAAGACGG 
     1141 TGGTCCTGCA GAAGAAAGAC AACGAGGGCT TTGGATTTGT GCTCCGAGGG GCAAAAGCTG 
     1201 ATACCCCCAT TGAGGAATTC ACACCCACGC CGGCATTTCC AGCCCTGCAG TACCTGGAGT 
     1261 CCGTGGATGA AGGAGGGGTG GCATGGCAAG CCGGACTAAG GACCGGGGAC TTCTTGATTG 
     1321 AGGTTAACAA TGAAAATGTC GTCAAGGTGG GCCACAGGCA GGTGGTGAAC ATGATCCGCC 
     1381 AGGGAGGGAA TCACCTCGTC CTTAAGGTCG TCACGGTGAC CAGGAATCTA GACCCTGATG 
     1441 ATACAGCCAG AAAGAAAGCT CCCCCACCTC CAAAGCGGGC TCCGACCACG GCCCTCACCC 
     1501 TGCGTTCCAA GTCCATGACA GCGGAATTGG AGGAACTCGT GGATAAAGCC TCAGTCCGGA 
     1561 AGAAGAAGGA TAAACCGGAA GAGATAGTCC CAGCCTCCAA GCCCTCCAGG ACTGCAGAGA 
     1621 ACGTGGCCAT AGAATCCAGG GTGGCAACCA TCAAGCAGCG GCCCACAAGC CGGTGCTTTC 
     1681 CAGCTGCCTC TGATGTGAAC TCCGTGTACG AGCGCCAAGG GATTGCTGTA ATGACGCCCA 
     1741 CGGTCCCTGG GAGCCCGAAA GGCCCATTTC TGGGCCTCCC TCGAGGTACG ATGCGAAGGC 
     1801 AGAAATCGAT AGACAGCAGA ATCTTTCTAT CAGGGATAAC AGAGGAAGAG CGGCAGTTTC 
     1861 TGGCTCCCCC AATGCTGAAG TTCACCCGAA GCTTGTCCAT GCCAGACACT TCTGAGGACA 
     1921 TCCCCCCTCC GCCACAGTCT GTGCCCCCCT CTCCCCCTCC ACCTTCCCCC ACCACATACA 
     1981 ACTGTCCCAG GTCCCCGACT CCAAGAGTCT ATGGGACAAT TAAGCCTGCG TTCAACCAGA 
     2041 ACCCCGTCGC CAAGGTGCCC CCAGCCACCA GGTCTGACAC TGTGGCCACC ATGATGCGGG 
     2101 AAAAGGGGAT GTTCTACAGG AGAGAGCTGG ACCGCTTTTC CCTGGACTCA GAAGACGTCT 
     2161 ACAGCCGCAG CCCCGCCCCA CAGGCTGCCT TCCGCACCAA GCGGGGACAG ATGCCTGAGA 
     2221 ACCCGTACTC GGAGGTGGGA AAGATAGCCA GCAAGGCCGT CTATGTCCCT GCCAAGCCAG 
     2281 CCAGGCGGAA GGGCATGCTG GTAAAGCAGT CCAACGTGGA GGACAGCCCT GAGAAGACGT 
     2341 GCTCCATCCC CATCCCAACC ATCATAGTCA AGGAACCCTC CACCAGCAGC AGCGGCAAGA 
     2401 GCAGCCAGGG GAGCAGCATG GAGATCGACC CCCAGGCCAC TGAGCCCGGC CAGCTGCGGC 
     2461 CAGATGACAG CCTCACCGTC AGCAGCCCCT TCGCGGCAGC CATCGCTGGG GCCGTGCGTG 
     2521 ACCGGGAGAA GCGTCTGGAA GCCAGGAGGA ATTCCCCGGC CTTCCTCTCC ACCGACCTGG 
     2581 GAGATGAGGA CGTGGGTCTG GGGCCTCCTG CTCCCCGGAT GCAGCCCTCC AAGTTTCCCG 
     2641 AGGAGGGTGG GTTTGGTGAT GAGGATGAAA CGGAACAGCC GCTATTGCCT ACCCCTGGAG 
     2701 CAGCGCCCAG GGAGCTGGAG AACCACTTCC TAGGTGGTGG TGAGGCTGGT GCTCAGGGGG 
     2761 AGGCTGGGGG ACCCCTGAGT TCCACATCCA AAGCCAAGGG GCCTGAAAGT GGCCCAGCAG 
     2821 CCGCCCTCAA GAGCAGCAGC CCAGCCAGCC CTGAGAATTA CGTGCACCCA CTCACGGGGC 
     2881 GGCTGCTCGA CCCCAGCTCC CCGCTGGCCC TGGCACTGTC CGCCAGGGAC CGAGCCATGC 
     2941 AGGAGTCCCA GCAAGGACAC AAGGGGGAGG CCCCCAAGGC CGACCTTAAC AAGCCTCTCT 
     3001 ACATCGATAC CAAAATGCGG CCCAGTGTGG AGTCCGGCTT TCCACCGGTC ACCAGACAGA 
     3061 ACACCAGGGG TCCCCTGCGA AGGCAGGAGA CAGAGAACAA GTATGAGACG GACCTGAGCA 
     3121 AGGACCGGAG GGCTGACGAC AAGAAGAACA TGCTGATCAA CATCGTGGAC ACTGCCCAGC 
     3181 AGAAGTCAGC CGGCCTACTG ATGGTGCACA CAGTGGACAT TCCCGTGGCG GGGCCACCCC 
     3241 TGGAGGAAGA GGAGGACAGA GAGGATGGGG ATACAAAGCC AGACCACTCA CCCTCCACAG 
     3301 TGCCAGAAGG CGTTCCCAAA ACCGAAGGTG CTTTACAGAT CTCCGCTGCC CCGGAGCCCG 
     3361 CCGCTGCGCC CGGCAGGACC ATCGTGGCAG CGGGCTCCGT GGAAGAGGCG GTAATTCTGC 
     3421 CATTCCGCAT CCCCCCTCCC CCTCTGGCGT CCGTGGACTT GGATGAGGAC TTTCTTTTCA 
     3481 CAGAACCATT GCCTCCTCCC CTGGAATTCG CCAATAGTTT TGATATCCCC GACGACCGGG 
     3541 CAGCTTCAGT TCCCGCTCTG GCTGACCTGG TCAAGCAGAA GAAAAGTGAC ACCCCTCAGC 
     3601 CCCCTTCGTT GAACTCCAGC CAACCAGCCA ACTCTACAGA CAGTAAGAAG CCAGCCGGCA 
     3661 TCTCAAACTG TCTGCCCTCC TCATTCCTGC CACCCCCCGA AAGCTTTGAC GCGGTCACCG 
     3721 ACTCGGGGAT CGAGGAGGTG GACAGCCGGA GTAGCAGCGA CCACCACCTG GAGACAACCA 
     3781 GCACCATCTC CACAGTGTCC AGCATCTCCA CGCTGTCTTC AGAGGGCGGG GAGAGCATGG 
     3841 ATACTTGCAC AGTCTATGCA GACGGGCAAG CCTTTGTGGT TGACAAGCCC CCAGTACCTC 
     3901 CAAAGCCAAA AATGAAGCCC ATCGTTCACA AAAGCAACGC ACTTTACCAA GACACGCTCC 
     3961 CAGAAGAGGA CACAGATGGC TTTGTGATCC CCCCACCTGC ACCCCCGCCC CCGCCCGGCA 
     4021 GTGCCCAGGC CGGTGTGGCG AAGGTCATCC AGCCAAGGAC CTCCAAGTTG TGGGGTGATG 
     4081 TTACAGAGGT CAAAAGCCCA ATTCTCTCAG GCCCAAAGGC AAATGTCATT AGTGAGTTAA 
     4141 ACTCCATTCT GCAGCAGATG AACAGGGGGA AATCGGTCAA GCCTGGGGAA GGGCTGGAGC 
     4201 TGCCCGTGGG AGCCAAGTCA GCTAACCTCG CTCCAAGAAG CCCGGAGGTC ATGAGCACTG 
     4261 TCTCAGGTAC ACGGAGCACG ACGGTCACCT TCACTGTCCG CCCCGGAACC TCCCAGCCCA 
     4321 TCACCCTACA GAGCCGGCCC CCTGACTATG AAAGCAGGAC CTCAGGACCG AGACGTGCCC 
     4381 CAAGCCCTGT GGTTTCGCCA ACGGAATTGA GCAAAGAGAT TCTGCCCACC CCTCCGTCTG 
     4441 CTGCAGCAGC CTCTCCCTCC CCCACACTCT CAGATGTCTT TAGCCTTCCA AGCCAGTCCC 
     4501 CTGCAGGGGA CCTCTTTGGC TTGAACCCAG CAGGACGCAG CAGGTCACCA TCTCCTTCAA 
     4561 TATTGCAACA GCCAATCTCA AATAAGCCTT TTACAACTAA GCCTGTCCAC CTGTGGACGA 
     4621 AACCAGATGT GGCAGACTGG CTGGAAAGTC TGAACTTGGG TGAACACAAG GAGACGTTCA 
     4681 TGGACAATGA GATTGACGGC AGCCATCTGC CAAACCTCCA GAAGGAAGAC TTGATAGATC 
     4741 TTGGGGTGAC TCGAGTTGGG CACAGGATGA ACATAGAAAG GGCTTTGAAA CAGCTGCTGG 
     4801 ACAGAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//