LOCUS       RR200180                8628 bp    DNA     circular  SYN 
DEFINITION  Syngap1 (Myc-DDK-tagged ORF) - Rat synaptic Ras GTPase activating protein 1 homolog (rat) (Syngap1), transcript variant 2, (10 ug)
ACCESSION   NM_001113409
VERSION     NM_001113409.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Syngap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTAAGG CCCCCCACCC CGACCCCGTC AGGGGGCTCC GGTTCAGGTT 
     1081 CCTTGCCCCC TCCTTCCCAC CGCCAGCCTC TCCGCCGCCG CTGCTCTTCC TGCTGCTTTC 
     1141 CGGGGGAATA CCACTTGGGT CGCTCGAGGA GGAAGAGTGT CCCCGGGGGG AAACAGTACA 
     1201 GCATGGAAGC CGCCCCCGCT GCGCCCTTCC GGCCCTCGCA AGGCTTCCTG AGCCGGAGGC 
     1261 TAAAAAGCTC CATCAAACGT ACAAAGTCAC AACCCAAACT TGACCGGACC AGCAGCTTTC 
     1321 GACAGATCCT GCCTCGCTTC CGAAGTGCTG ACCATGACCG GGCCCGGCTG ATGCAGAGCT 
     1381 TCAAGGAGTC TCACTCCCAT GAGTCCCTGC TGAGTCCCAG CAGTGCTGCT GAGGCCCTGG 
     1441 AGCTCAACCT GGATGAAGAC TCCATTATCA AGCCAGTACA CAGCTCCATC CTGGGCCAGG 
     1501 AGTTCTGCTT TGAGGTAACA ACATCGTCTG GGACAAAATG TTTTGCCTGT CGGTCTGCAG 
     1561 CCGAAAGGGA CAAATGGATT GAGAATCTAC AGAGGGCTGT GAAACCCAAC AAGGACAACA 
     1621 GCCGCCGGGT AGATAACGTG CTGAAACTAT GGATCATAGA AGCTCGAGAG CTGCCCCCCA 
     1681 AGAAGCGATA TTACTGCGAG TTATGCCTGG ACGACATGCT CTATGCACGG ACCACTTCCA 
     1741 AGCCCCGCTC AGCCTCAGGA GACACTGTCT TTTGGGGCGA GCACTTCGAG TTTAACAACC 
     1801 TGCCTGCTGT CCGGGCGCTG CGGCTGCATC TGTACCGTGA CTCGGACAAA AAGCGGAAGA 
     1861 AGGACAAGGC AGGCTACGTT GGCCTGGTGA CTGTTCCAGT GGCCACCCTG GCTGGGCGCC 
     1921 ACTTCACAGA GCAGTGGTAC CCCGTGACCC TGCCAACAGG AAGTGGGGGC TCTGGGGGTA 
     1981 TGGGCTCGGG GGGAGGAGGG GGGTCAGGGG GCGGCTCAGG GGGCAAAGGG AAAGGAGGCT 
     2041 GTCCTGCTGT GCGGCTGAAG GCCCGTTACC AGACAATGAG TATCCTGCCC ATGGAGCTAT 
     2101 ATAAGGAGTT TGCAGAATAT GTGACCAACC ACTACCGCAT GCTGTGTGCC GTGCTGGAGC 
     2161 CCGCCCTCAA TGTCAAGGGC AAGGAGGAGG TCGCTAGTGC ACTGGTTCAC ATCCTGCAAA 
     2221 GCACAGGCAA GGCCAAGGAC TTCCTTTCAG ACATGGCCAT GTCAGAGGTA GACCGGTTCA 
     2281 TGGAGCGGGA ACACCTCATA TTCCGCGAGA ACACGCTCGC CACTAAAGCC ATAGAAGAGT 
     2341 ATATGAGACT GATTGGCCAG AAATACCTCA AGGATGCCAT TGGGGAGTTC ATCCGGGCTC 
     2401 TGTATGAATC TGAGGAGAAC TGTGAAGTAG ACCCCATCAA GTGCACAGCG TCCAGTCTGG 
     2461 CAGAGCACCA GGCCAACCTG CGGATGTGCT GTGAGTTGGC CCTGTGCAAG GTGGTCAACT 
     2521 CCCATTGCGT GTTCCCGGGG GAGCTGAAGG AGGTGTTTGC ATCATGGCGG CTGCGCTGTG 
     2581 CAGAGCGGGG CCGGGAGGAC ATTGCTGACA GGCTGATCAG CGCCTCGCTC TTCCTGCGCT 
     2641 TCCTCTGCCC GGCCATCATG TCGCCCAGTC TGTTTGGACT GATGCAGGAG TACCCAGATG 
     2701 AGCAGACCTC ACGAACCCTC ACCCTCATCG CCAAGGTTAT CCAGAACCTG GCCAACTTTT 
     2761 CCAAGTTTAC CTCAAAGGAG GACTTCCTGG GCTTCATGAA CGAGTTTCTG GAGCTGGAGT 
     2821 GGGGTTCTAT GCAGCAATTC TTGTATGAGA TATCCAACCT GGACACACTG ACCAACAGCA 
     2881 GCAGTTTTGA GGGCTACATA GACTTGGGCC GCGAGCTCTC CACACTTCAC GCCCTGCTCT 
     2941 GGGAGGTGCT GCCCCAGCTC AGCAAGGAAG CCCTCCTGAA GCTGGGCCCG CTGCCCCGGC 
     3001 TCCTCAGCGA CATCAGCACA GCCCTGAGGA ACCCTAACAT CCAAAGGCAG CCGAGCCGCC 
     3061 AGAGCGAGCG CGCTCGGTCT CAGCCCATGG TGCTGCGCGG GCCGTCAGCC GAGATGCAGG 
     3121 GCTACATGAT GCGGGACCTC AACAGCTCCA TCGACCTTCA GTCCTTCATG GCTCGAGGCC 
     3181 TCAACAGCTC TATGGACATG GCTCGCCTCC CCTCCCCAAC CAAGGAGAAA CCCCCGCCGC 
     3241 CCCCTCCCGG TGGGGGTAAA GACCTGTTCT ATGTGAGCCG GCCACCACTG GCCCGGTCCT 
     3301 CCCCAGCATA CTGCACGAGC AGCTCGGACA TCACAGAGCC GGAGCAGAAG ATGCTGAGTG 
     3361 TCAACAAGAG TGTGTCCATG CTGGACCTGC AGGGCGACGG GCCTGGGGGC CGCCTTAACA 
     3421 GCAGTAGTGT TTCCAACCTG GCAGCTGTTG GGGACCTGTT GCACTCAAGC CAGGCTTCAC 
     3481 TGACAGCAGC CTTGGGGTTG CGGCCTGCAC CTGCCGGGCG CCTCTCCCAA GGGAGTGGCT 
     3541 CTTCCATCAC AGCAGCCGGC ATGCGCCTCA GCCAGATGGG TGTCACTACG GATGGTGTCC 
     3601 CCGCCCAGCA ACTGCGCATC CCTCTTTCCT TCCAGAACCC TCTCTTCCAT ATGGCTGCCG 
     3661 ATGGACCAGG GCCCCCAGCA GGCCATGGAG GGAGCAGTGG CCATGGTCCA CCTTCCTCCC 
     3721 ATCACCACCA CCACCACCAT CACCATCACC GAGGGGGAGA ACCCCCAGGG GACACTTTTG 
     3781 CCCCGTTCCA TGGCTATAGC AAGAGCGAGG ACCTCTCTAC AGGGGTCCCT AAGCCCCCTG 
     3841 CGGCCTCCAT CCTTCACAGC CACAGCTACA GTGATGAGTT TGGACCCTCT GGTACTGATT 
     3901 TTACCCGTCG GCAGCTCTCA CTTCAGGACA ACCTACAGCA CATGCTCTCC CCGCCCCAGA 
     3961 TCACCATCGG TCCCCAGAGG CCAGCTCCCT CAGGGCCAGG AGGGGGCAGT GGTGGGGGCA 
     4021 GTGGTGGGGG CGGTGGGGGC CAGCCACCTC CCTTGCAGAG GGGCAAATCT CAGCAGTTGA 
     4081 CAGTGAGTGC TGCCCAGAAA CCCCGGCCGT CCAGCGGGAA CCTATTGCAG TCCCCGGAAC 
     4141 CAAGTTATGG TCCTGCCCGT CCACGGCAAC AGAGCCTCAG CAAAGAGGGC AGCATTGGGG 
     4201 GCAGCGGGGG CAGCGGTGGC GGAGGGGGTG GGGGGCTCAA GCCCTCCATC ACCAAGCAGC 
     4261 ATTCCCAGAC TCCATCCACG CTGAACCCCA CGATGCCGGC CTCGGAGCGG ACTGTAGCCT 
     4321 GGGTGTCCAA TATGCCTCAC CTGTCCGCTG ACATCGAGAG TGCACACATT GAGCGGGAAG 
     4381 AGTACAAGCT GAAGGAGTAC TCGAAGTCCA TGGACGAGAG CCGACTGGAC AGGGTGAAGG 
     4441 AGTACGAGGA GGAGATCCAC TCACTGAAGG AAAGGCTACA CATGTCCAAC CGGAAGCTGG 
     4501 AAGAGTACGA GCGGAGGCTG CTGTCCCAGG AAGAGCAGAC CAGCAAGATC CTGATGCAGT 
     4561 ACCAAGCCCG CCTGGAGCAG AGCGAGAAGC GCTTGAGGCA GCAGCAGGTG GAGAAGGACT 
     4621 CCCAGATCAA GAGCATCATT GGCAGGCTGA TGCTGGTGGA GGAGGAGCTG CGCCGGGACC 
     4681 ACCCCGCCAT GGCTGAGCCG CTGCCTGAAC CCAAGAAGAG GCTGCTCGAC GCTCAGAGAG 
     4741 GCAGCTTCCC CCCTTGGGTC CAACAAACCC GCGTGACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//