LOCUS       RR200179                8805 bp    DNA     circular  SYN 
DEFINITION  Syngap1 (Myc-DDK-tagged ORF) - Rat synaptic Ras GTPase activating protein 1 homolog (rat) (Syngap1), transcript variant 1, (10 ug)
ACCESSION   NM_181092
VERSION     NM_181092.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4962..5053 
                     /label="Myc-DDK"  
     misc_feature    5083..5668 
                     /label="PolyA signal"  
     misc_feature    5140..5159 
                     /label="Restriction Enzyme"  
     misc_feature    5944..6616 
                     /label="ColE1"  
     misc_feature    7046..7840 
                     /label="Kan/Neo"  
     misc_feature    7875..8177 
                     /label="SV40 ori"  
     misc_feature    8263..8740 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4944..4950 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4944 
                     /label="Syngap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCAGGTCT CGAGCCTCCA TCCATCGGGG GAGCATCCCC GCGATGTCCT 
     1081 ATGCCCCCTT CAGAGATGTA CGGGGACCCC CTATGCACCG AACCCAATAC GTTCATTCCC 
     1141 CGTATGACCG TCCCGGTTGG AACCCCCGGT TCTGCATCAT CTCTGGGAAC CAGCTGCTCA 
     1201 TGCTGGATGA GGATGAGATA CACCCCCTTC TGATCCGCGA CCGGAGGAGC GAGTCCAGCC 
     1261 GAAACAAACT GCTGAGACGC ACCGTCTCTG TGCCAGTGGA GGGGCGGCCC CACGGCGAGC 
     1321 ATGAATACCA CTTGGGTCGC TCGAGGAGGA AGAGTGTCCC CGGGGGGAAA CAGTACAGCA 
     1381 TGGAAGCCGC CCCCGCTGCG CCCTTCCGGC CCTCGCAAGG CTTCCTGAGC CGGAGGCTAA 
     1441 AAAGCTCCAT CAAACGTACA AAGTCACAAC CCAAACTTGA CCGGACCAGC AGCTTTCGAC 
     1501 AGATCCTGCC TCGCTTCCGA AGTGCTGACC ATGACCGGGC CCGGCTGATG CAGAGCTTCA 
     1561 AGGAGTCTCA CTCCCATGAG TCCCTGCTGA GTCCCAGCAG TGCTGCTGAG GCCCTGGAGC 
     1621 TCAACCTGGA TGAAGACTCC ATTATCAAGC CAGTACACAG CTCCATCCTG GGCCAGGAGT 
     1681 TCTGCTTTGA GGTAACAACA TCGTCTGGGA CAAAATGTTT TGCCTGTCGG TCTGCAGCCG 
     1741 AAAGGGACAA ATGGATTGAG AATCTACAGA GGGCTGTGAA ACCCAACAAG GACAACAGCC 
     1801 GCCGGGTAGA TAACGTGCTG AAACTATGGA TCATAGAAGC TCGAGAGCTG CCCCCCAAGA 
     1861 AGCGATATTA CTGCGAGTTA TGCCTGGACG ACATGCTCTA TGCACGGACC ACTTCCAAGC 
     1921 CCCGCTCAGC CTCAGGAGAC ACTGTCTTTT GGGGCGAGCA CTTCGAGTTT AACAACCTGC 
     1981 CTGCTGTCCG GGCGCTGCGG CTGCATCTGT ACCGTGACTC GGACAAAAAG CGGAAGAAGG 
     2041 ACAAGGCAGG CTACGTTGGC CTGGTGACTG TTCCAGTGGC CACCCTGGCT GGGCGCCACT 
     2101 TCACAGAGCA GTGGTACCCC GTGACCCTGC CAACAGGAAG TGGGGGCTCT GGGGGTATGG 
     2161 GCTCGGGGGG AGGAGGGGGG TCAGGGGGCG GCTCAGGGGG CAAAGGGAAA GGAGGCTGTC 
     2221 CTGCTGTGCG GCTGAAGGCC CGTTACCAGA CAATGAGTAT CCTGCCCATG GAGCTATATA 
     2281 AGGAGTTTGC AGAATATGTG ACCAACCACT ACCGCATGCT GTGTGCCGTG CTGGAGCCCG 
     2341 CCCTCAATGT CAAGGGCAAG GAGGAGGTCG CTAGTGCACT GGTTCACATC CTGCAAAGCA 
     2401 CAGGCAAGGC CAAGGACTTC CTTTCAGACA TGGCCATGTC AGAGGTAGAC CGGTTCATGG 
     2461 AGCGGGAACA CCTCATATTC CGCGAGAACA CGCTCGCCAC TAAAGCCATA GAAGAGTATA 
     2521 TGAGACTGAT TGGCCAGAAA TACCTCAAGG ATGCCATTGG GGAGTTCATC CGGGCTCTGT 
     2581 ATGAATCTGA GGAGAACTGT GAAGTAGACC CCATCAAGTG CACAGCGTCC AGTCTGGCAG 
     2641 AGCACCAGGC CAACCTGCGG ATGTGCTGTG AGTTGGCCCT GTGCAAGGTG GTCAACTCCC 
     2701 ATTGCGTGTT CCCGGGGGAG CTGAAGGAGG TGTTTGCATC ATGGCGGCTG CGCTGTGCAG 
     2761 AGCGGGGCCG GGAGGACATT GCTGACAGGC TGATCAGCGC CTCGCTCTTC CTGCGCTTCC 
     2821 TCTGCCCGGC CATCATGTCG CCCAGTCTGT TTGGACTGAT GCAGGAGTAC CCAGATGAGC 
     2881 AGACCTCACG AACCCTCACC CTCATCGCCA AGGTTATCCA GAACCTGGCC AACTTTTCCA 
     2941 AGTTTACCTC AAAGGAGGAC TTCCTGGGCT TCATGAACGA GTTTCTGGAG CTGGAGTGGG 
     3001 GTTCTATGCA GCAATTCTTG TATGAGATAT CCAACCTGGA CACACTGACC AACAGCAGCA 
     3061 GTTTTGAGGG CTACATAGAC TTGGGCCGCG AGCTCTCCAC ACTTCACGCC CTGCTCTGGG 
     3121 AGGTGCTGCC CCAGCTCAGC AAGGAAGCCC TCCTGAAGCT GGGCCCGCTG CCCCGGCTCC 
     3181 TCAGCGACAT CAGCACAGCC CTGAGGAACC CTAACATCCA AAGGCAGCCG AGCCGCCAGA 
     3241 GCGAGCGCGC TCGGTCTCAG CCCATGGTGC TGCGCGGGCC GTCAGCCGAG ATGCAGGGCT 
     3301 ACATGATGCG GGACCTCAAC AGCTCCATCG ACCTTCAGTC CTTCATGGCT CGAGGCCTCA 
     3361 ACAGCTCTAT GGACATGGCT CGCCTCCCCT CCCCAACCAA GGAGAAACCC CCGCCGCCCC 
     3421 CTCCCGGTGG GGGTAAAGAC CTGTTCTATG TGAGCCGGCC ACCACTGGCC CGGTCCTCCC 
     3481 CAGCATACTG CACGAGCAGC TCGGACATCA CAGAGCCGGA GCAGAAGATG CTGAGTGTCA 
     3541 ACAAGAGTGT GTCCATGCTG GACCTGCAGG GCGACGGGCC TGGGGGCCGC CTTAACAGCA 
     3601 GTAGTGTTTC CAACCTGGCA GCTGTTGGGG ACCTGTTGCA CTCAAGCCAG GCTTCACTGA 
     3661 CAGCAGCCTT GGGGTTGCGG CCTGCACCTG CCGGGCGCCT CTCCCAAGGG AGTGGCTCTT 
     3721 CCATCACAGC AGCCGGCATG CGCCTCAGCC AGATGGGTGT CACTACGGAT GGTGTCCCCG 
     3781 CCCAGCAACT GCGCATCCCT CTTTCCTTCC AGAACCCTCT CTTCCATATG GCTGCCGATG 
     3841 GACCAGGGCC CCCAGCAGGC CATGGAGGGA GCAGTGGCCA TGGTCCACCT TCCTCCCATC 
     3901 ACCACCACCA CCACCATCAC CATCACCGAG GGGGAGAACC CCCAGGGGAC ACTTTTGCCC 
     3961 CGTTCCATGG CTATAGCAAG AGCGAGGACC TCTCTACAGG GGTCCCTAAG CCCCCTGCGG 
     4021 CCTCCATCCT TCACAGCCAC AGCTACAGTG ATGAGTTTGG ACCCTCTGGT ACTGATTTTA 
     4081 CCCGTCGGCA GCTCTCACTT CAGGACAACC TACAGCACAT GCTCTCCCCG CCCCAGATCA 
     4141 CCATCGGTCC CCAGAGGCCA GCTCCCTCAG GGCCAGGAGG GGGCAGTGGT GGGGGCAGTG 
     4201 GTGGGGGCGG TGGGGGCCAG CCACCTCCCT TGCAGAGGGG CAAATCTCAG CAGTTGACAG 
     4261 TGAGTGCTGC CCAGAAACCC CGGCCGTCCA GCGGGAACCT ATTGCAGTCC CCGGAACCAA 
     4321 GTTATGGTCC TGCCCGTCCA CGGCAACAGA GCCTCAGCAA AGAGGGCAGC ATTGGGGGCA 
     4381 GCGGGGGCAG CGGTGGCGGA GGGGGTGGGG GGCTCAAGCC CTCCATCACC AAGCAGCATT 
     4441 CCCAGACTCC ATCCACGCTG AACCCCACGA TGCCGGCCTC GGAGCGGACT GTAGCCTGGG 
     4501 TGTCCAATAT GCCTCACCTG TCCGCTGACA TCGAGAGTGC ACACATTGAG CGGGAAGAGT 
     4561 ACAAGCTGAA GGAGTACTCG AAGTCCATGG ACGAGAGCCG ACTGGACAGG GTGAAGGAGT 
     4621 ACGAGGAGGA GATCCACTCA CTGAAGGAAA GGCTACACAT GTCCAACCGG AAGCTGGAAG 
     4681 AGTACGAGCG GAGGCTGCTG TCCCAGGAAG AGCAGACCAG CAAGATCCTG ATGCAGTACC 
     4741 AAGCCCGCCT GGAGCAGAGC GAGAAGCGCT TGAGGCAGCA GCAGGTGGAG AAGGACTCCC 
     4801 AGATCAAGAG CATCATTGGC AGGCTGATGC TGGTGGAGGA GGAGCTGCGC CGGGACCACC 
     4861 CCGCCATGGC TGAGCCGCTG CCTGAACCCA AGAAGAGGCT GCTCGACGCT CAGAGAGGCA 
     4921 GCTTCCCCCC TTGGGTCCAA CAAACCCGCG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4981 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5041 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5101 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5161 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5221 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5281 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5341 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5401 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5461 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5521 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5581 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5641 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5701 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5761 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5821 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5881 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5941 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6001 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6061 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6121 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6181 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6241 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6301 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6361 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6421 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6481 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6541 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6601 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6661 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6721 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6781 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6841 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6901 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6961 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7021 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7081 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7141 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7201 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7261 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7321 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7381 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7441 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7501 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7561 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7621 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7681 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7741 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7801 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7861 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7921 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7981 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8041 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8221 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8341 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8401 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8461 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8521 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8581 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8641 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8701 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8761 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//