LOCUS       RR200175                8655 bp    DNA     circular  SYN 
DEFINITION  Cdk12 (Myc-DDK-tagged ORF) - Rat Cdc2-related kinase, arginine/serine-rich (Crkrs), transcript variant 2, (10 ug)
ACCESSION   NM_138916
VERSION     NM_138916.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4812..4903 
                     /label="Myc-DDK"  
     misc_feature    4933..5518 
                     /label="PolyA signal"  
     misc_feature    4990..5009 
                     /label="Restriction Enzyme"  
     misc_feature    5794..6466 
                     /label="ColE1"  
     misc_feature    6896..7690 
                     /label="Kan/Neo"  
     misc_feature    7725..8027 
                     /label="SV40 ori"  
     misc_feature    8113..8590 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4794..4800 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4794 
                     /label="Cdk12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAATTCA GAGAGACATG GGGGCAAGAA GGACGGGAGT GGAGGAGCTT 
     1081 CTGGAACTTC GCAGCCGTCA TCGGGAGGCG GCAGCTCCAA CAGCAGGGAG CGTCACCGCT 
     1141 TGGTGTCGAA GCACAAGCGG CATAAGTCCA AGCACTCCAA AGACATGGGG TTGGTAACCC 
     1201 CCGAAGCGGC ATCTTTGGGT ACAATAATCA AACCATTGGT GGAGTATGAT GACATCAGCT 
     1261 CTGATTCAGA CACCTTCTCA GATGACATGG CCTTCAAATC AGACAGGAGG GAGAACGATG 
     1321 AACGTCGTGG AACGGATCGG AGTGACCGCC TGCACAGACA TCGTCACCAC CAGCACCGGC 
     1381 GGTCCCGAGA CTTGTTAAAA ACTAAACAGA CCGAAAAGGA AAAAAACCAG GAAGTCTCCA 
     1441 AATCTGGATC GATGAAGGAC CGGTTATCAG GTAGTTCGAA ACGTTCAGTG GAGGGGAACG 
     1501 ATGATTATGG GAAGGCACAA TTATCCAAAA GCAGCAGCAA GGAATCCAGG TCGTCCAAAA 
     1561 TGCACAAAGA GAAGACCCGG AAAGAACGAG AGTTAAAGTC TGGACACAAG GACCGTAGTA 
     1621 AAAGTCATCG GAAAAGGGAA ACACCCAAAA GTTACAAAAC GGTGGATAGC CCCAAACGGA 
     1681 GATCTAGGAG TCCCCATAGG AAATGGTCTG ACAGTTCCAA GCAAGATGAC AGCCCTTCTG 
     1741 GAGCTTCTTA TGGCCAAGAT TACGATCTTA GCCCCCCAAG GTCTCATACT TCTAGCAATT 
     1801 ATGACTCCTA CAAGAAGAGT CCGGGAAGTA CCTCAAGAAG GCAGTCAATC AGCCCACCTT 
     1861 ATAAAGAGCC TTCTGCTTAC CAGTCCAGCA CTCGGTCACC CAGTCCCTAC AGCCGACGAC 
     1921 AGAGGTCAGT GAGCCCCTAT AGCCGGAGAC GGTCATCCAG CTATGAAAGG AGCGGTTCTT 
     1981 ACAGCGGGAG ATCACCCAGC CCCTATGGCC GAAGGCGATC TAGCAGCCCT TTCTTGAGCA 
     2041 AGAGGTCTCT GAGTCGGAGT CCAATCTCCA GTAGGAAATC CATGAAGTCC AGAAGTAGAA 
     2101 GTCCTGCATA TTCAAGACAC TCATCTTCAC ATAGTAAAAA GAAGCGATCC GGGTCACGAA 
     2161 GTCGTCATTC CAGCATCTCA CCTGTCAGGC TTCCATTGAA TTCCAGCCTG GGAGCTGAAC 
     2221 TCAGTAGAAA AAAGAAGGAA AGAGCAGCTG CTGCAGCAGC AGCAAAATTG GATGGAAAAG 
     2281 AATCTAAGGG TTCACCTATA ATTTTGCCTA AAAAAGAGAA ATTTGAGGTG AAGGAGTCAG 
     2341 GGTTAGAGTC TAAAAAGTTA CCCAGAGGTA TAAAGTCAGA AAAATCTACC CCAGATACTG 
     2401 AACTGGTAAA TGTAGCACAT TCAAACACAG AGGTAAAAAA TTGTTTAGAT ACAGGGAAAG 
     2461 TAAAGTTGGA TGAGAACTTG CAGAAGCATC CTGTTAAGGA TTTGAAAGCA CAGGGAACAA 
     2521 AGGACACCAA ACCTGTAGCA CTGAAGGAGG TGATTGTTAC TTCAAAGGAG ACAGAGACAT 
     2581 CAGAAAAGGA GGCCCTTCCA CCTCTCCCCA CAATAACTTC TCCACCCCCT TTACCATCTA 
     2641 CTACTCCTCC ACCTCAGACA CCCCCTTTGC CACCTTTGCC TCCACTACCA GCTGTTCCAC 
     2701 TGCAGCCACC TTTGCCTCCT CCCCAACCAC CATTTAGTCA AGTTCCTGTT TCAAATACTT 
     2761 CAACTTTACC CTCTTCTCCT CACCCAAGGA CATCTACTTT ATCCTCTCAG ACAAATTCTC 
     2821 AGCCCCTTGT ACAGGTTTCT ATGAAGACTC AATTATCTGT AACAGCTGCT ATTCCACACC 
     2881 TGAAAACTTC AACATTGCCT CCTCTGCCCC TCCCTCCCCT ATTACCTGGA GATGATGACA 
     2941 TGGATAGTCC AAAAGAAATG CTTCCTTCAA AGCCTGCAAA GAAAGAGAAA GAGCAGAGGA 
     3001 CTCGCCACTT ACTCACAGAC TTGCCTCTTC CTCCTGAGCT ACCAGGTGGA GATCCATCTC 
     3061 CCCCAGATTC TCCAGAGCCA AAGGCAATTA CACCACCTCA ACAACCATAT AAAAAGAGAC 
     3121 CAAAAATTTG TTGTCCACGT TATGGAGAAA GAAGACAAAC AGAAAGTGAT TGGGGGAAGC 
     3181 GCTGCGTGGA CAAGTTTGAC ATTATTGGGA TTATTGGAGA AGGAACCTAT GGCCAAGTAT 
     3241 ATAAAGCCAA GGACAAAGAC ACAGGAGAAC TTGTAGCTCT GAAGAAGGTT CGGCTAGACA 
     3301 ATGAGAAAGA GGGCTTCCCA ATCACAGCCA TCCGGGAGAT TAAGATTCTT CGTCAGTTAG 
     3361 TTCATCAAAG TGTTGTAAAC ATGAAGGAGA TTGTCACAGA CAAACAAGAT GCACTGGATT 
     3421 TCAAGAAGGA CAAAGGTGCC TTTTACCTTG TATTTGAATA TATGGACCAT GACTTAATGG 
     3481 GACTGCTAGA ATCAGGTTTG GTGCACTTTT CTGAGGACCA TATCAAGTCA TTCATGAAAC 
     3541 AGCTAATGGA AGGACTGGAT TACTGTCACA AAAAGAATTT CCTCCATCGG GATATTAAAT 
     3601 GTTCTAACAT TTTGCTGAAT AACAGTGGGC AAATCAAACT GGCAGATTTT GGCCTTGCTC 
     3661 GGCTCTATAA CTCTGAAGAG AGTCGCCCTT ACACAAACAA AGTCATTACT CTGTGGTATC 
     3721 GACCTCCAGA GCTGCTTCTT GGAGAGGAAA GATACACACC AGCCATTGAT GTTTGGAGCT 
     3781 GTGGGTGCAT CCTTGGGGAA CTGTTCACAA AGAAACCTAT TTTTCAAGCC AATTTGGAAC 
     3841 TGGCTCAGCT AGAACTGATC AGTCGTCTCT GTGGTAGTCC TTGTCCAGCA GTGTGGCCTG 
     3901 ATGTTATCAA GCTGCCCTAC TTCAACACCA TGAAACCGAA AAAGCAATAC AGGAGACGCC 
     3961 TAAGAGAAGA ATTCTCTTTC ATTCCTTCAG CGGCACTTGA TCTGTTGGAC CACATGCTGA 
     4021 CACTGGATCC TAGCAAGCGC TGCACAGCGG AACAAACCCT ACAGAGTGAC TTTCTTAAAG 
     4081 ATGTGGAACT CAGCAAAATG GCACCTCCAG ACCTACCTCA CTGGCAGGAT TGCCATGAAT 
     4141 TGTGGAGTAA GAAACGTCGA CGGCAACGAC AAAGTGGTAT TGTGATAGAA GAGCAACCTC 
     4201 CATCCAAAGC TTCTCGAAAA GAAACTACCT CAGGGACAGC AGCTGAGCCT GTGAAGAACA 
     4261 GTAGCCCAGC ACCACCTCAA CCTGCTCCTG TCAAGGCAGA GCCTGGTCCA GGGGATGCAG 
     4321 TAGGGCTTGG TGACATCACA CAGCAGTTGA ATCAAAGTGA ATTGGCAGTG TTATTAAACC 
     4381 TGCTTCAGAG CCAAACTGAC CTGAGCATCC CTCAGATGGC ACAATTGCTT AACATCCACT 
     4441 CCAATCCAGA GATGCAACAG CAGCTTGAAG CCCTGAATCA GTCTATTAGT GCACTGACTG 
     4501 AAGCCAGTTC CCAGCAGCAA GACTCAGAAT CCATAGCCCC AGAGGAATCA TTGAAGGAGG 
     4561 TACCTTCTGT ATCTGTGGTC CTGCCTCCTG CTGAACAGAC AACTCCTGAA GCTTCAAACA 
     4621 CACCAGCTGA CATGCAGAAT ATGTTGGCAG TTCTCTTGAG TCAGCTGATG AAAACCCAAG 
     4681 AGCCAGCAGG TAACCTGGAG GAAAACACCA GTGACAAGAA TAGTGGGCCA CAGGGGCCCC 
     4741 GAAGAACCCC TACAATGCCA CAGGAGGAGG CAGCAGGTAA ACAGACCGGT CATGAATCTC 
     4801 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4861 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4921 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4981 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5041 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5101 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5161 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5221 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5281 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5341 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5401 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5461 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5521 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5581 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5641 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5701 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6481 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6541 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6601 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6661 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6721 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6781 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6841 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6901 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6961 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7021 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7081 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7141 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7201 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7261 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7321 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7381 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7441 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7501 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7561 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7621 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7681 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7741 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7801 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7861 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8221 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8281 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8341 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8401 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8461 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8521 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8581 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8641 TTTAACGCGA ATTTT
//