LOCUS       RR200174                9333 bp    DNA     circular  SYN 
DEFINITION  Cdk12 (Myc-DDK-tagged ORF) - Rat Cdc2-related kinase, arginine/serine-rich (Crkrs), transcript variant 1, (10 ug)
ACCESSION   NM_001033867
VERSION     NM_001033867.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5490..5581 
                     /label="Myc-DDK"  
     misc_feature    5611..6196 
                     /label="PolyA signal"  
     misc_feature    5668..5687 
                     /label="Restriction Enzyme"  
     misc_feature    6472..7144 
                     /label="ColE1"  
     misc_feature    7574..8368 
                     /label="Kan/Neo"  
     misc_feature    8403..8705 
                     /label="SV40 ori"  
     misc_feature    8791..9268 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5472..5478 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5472 
                     /label="Cdk12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAATTCA GAGAGACATG GGGGCAAGAA GGACGGGAGT GGAGGAGCTT 
     1081 CTGGAACTTC GCAGCCGTCA TCGGGAGGCG GCAGCTCCAA CAGCAGGGAG CGTCACCGCT 
     1141 TGGTGTCGAA GCACAAGCGG CATAAGTCCA AGCACTCCAA AGACATGGGG TTGGTAACCC 
     1201 CCGAAGCGGC ATCTTTGGGT ACAATAATCA AACCATTGGT GGAGTATGAT GACATCAGCT 
     1261 CTGATTCAGA CACCTTCTCA GATGACATGG CCTTCAAATC AGACAGGAGG GAGAACGATG 
     1321 AACGTCGTGG AACGGATCGG AGTGACCGCC TGCACAGACA TCGTCACCAC CAGCACCGGC 
     1381 GGTCCCGAGA CTTGTTAAAA ACTAAACAGA CCGAAAAGGA AAAAAACCAG GAAGTCTCCA 
     1441 AATCTGGATC GATGAAGGAC CGGTTATCAG GTAGTTCGAA ACGTTCAGTG GAGGGGAACG 
     1501 ATGATTATGG GAAGGCACAA TTATCCAAAA GCAGCAGCAA GGAATCCAGG TCGTCCAAAA 
     1561 TGCACAAAGA GAAGACCCGG AAAGAACGAG AGTTAAAGTC TGGACACAAG GACCGTAGTA 
     1621 AAAGTCATCG GAAAAGGGAA ACACCCAAAA GTTACAAAAC GGTGGATAGC CCCAAACGGA 
     1681 GATCTAGGAG TCCCCATAGG AAATGGTCTG ACAGTTCCAA GCAAGATGAC AGCCCTTCTG 
     1741 GAGCTTCTTA TGGCCAAGAT TACGATCTTA GCCCCCCAAG GTCTCATACT TCTAGCAATT 
     1801 ATGACTCCTA CAAGAAGAGT CCGGGAAGTA CCTCAAGAAG GCAGTCAATC AGCCCACCTT 
     1861 ATAAAGAGCC TTCTGCTTAC CAGTCCAGCA CTCGGTCACC CAGTCCCTAC AGCCGACGAC 
     1921 AGAGGTCAGT GAGCCCCTAT AGCCGGAGAC GGTCATCCAG CTATGAAAGG AGCGGTTCTT 
     1981 ACAGCGGGAG ATCACCCAGC CCCTATGGCC GAAGGCGATC TAGCAGCCCT TTCTTGAGCA 
     2041 AGAGGTCTCT GAGTCGGAGT CCAATCTCCA GTAGGAAATC CATGAAGTCC AGAAGTAGAA 
     2101 GTCCTGCATA TTCAAGACAC TCATCTTCAC ATAGTAAAAA GAAGCGATCC GGGTCACGAA 
     2161 GTCGTCATTC CAGCATCTCA CCTGTCAGGC TTCCATTGAA TTCCAGCCTG GGAGCTGAAC 
     2221 TCAGTAGAAA AAAGAAGGAA AGAGCAGCTG CTGCAGCAGC AGCAAAATTG GATGGAAAAG 
     2281 AATCTAAGGG TTCACCTATA ATTTTGCCTA AAAAAGAGAA ATTTGAGGTG AAGGAGTCAG 
     2341 GGTTAGAGTC TAAAAAGTTA CCCAGAGGTA TAAAGTCAGA AAAATCTACC CCAGATACTG 
     2401 AACTGGTAAA TGTAGCACAT TCAAACACAG AGGTAAAAAA TTGTTTAGAT ACAGGGAAAG 
     2461 TAAAGTTGGA TGAGAACTTG CAGAAGCATC CTGTTAAGGA TTTGAAAGCA CAGGGAACAA 
     2521 AGGACACCAA ACCTGTAGCA CTGAAGGAGG TGATTGTTAC TTCAAAGGAG ACAGAGACAT 
     2581 CAGAAAAGGA GGCCCTTCCA CCTCTCCCCA CAATAACTTC TCCACCCCCT TTACCATCTA 
     2641 CTACTCCTCC ACCTCAGACA CCCCCTTTGC CACCTTTGCC TCCACTACCA GCTGTTCCAC 
     2701 TGCAGCCACC TTTGCCTCCT CCCCAACCAC CATTTAGTCA AGTTCCTGTT TCAAATACTT 
     2761 CAACTTTACC CTCTTCTCCT CACCCAAGGA CATCTACTTT ATCCTCTCAG ACAAATTCTC 
     2821 AGCCCCTTGT ACAGGTTTCT ATGAAGACTC AATTATCTGT AACAGCTGCT ATTCCACACC 
     2881 TGAAAACTTC AACATTGCCT CCTCTGCCCC TCCCTCCCCT ATTACCTGGA GATGATGACA 
     2941 TGGATAGTCC AAAAGAAATG CTTCCTTCAA AGCCTGCAAA GAAAGAGAAA GAGCAGAGGA 
     3001 CTCGCCACTT ACTCACAGAC TTGCCTCTTC CTCCTGAGCT ACCAGGTGGA GATCCATCTC 
     3061 CCCCAGATTC TCCAGAGCCA AAGGCAATTA CACCACCTCA ACAACCATAT AAAAAGAGAC 
     3121 CAAAAATTTG TTGTCCACGT TATGGAGAAA GAAGACAAAC AGAAAGTGAT TGGGGGAAGC 
     3181 GCTGCGTGGA CAAGTTTGAC ATTATTGGGA TTATTGGAGA AGGAACCTAT GGCCAAGTAT 
     3241 ATAAAGCCAA GGACAAAGAC ACAGGAGAAC TTGTAGCTCT GAAGAAGGTT CGGCTAGACA 
     3301 ATGAGAAAGA GGGCTTCCCA ATCACAGCCA TCCGGGAGAT TAAGATTCTT CGTCAGTTAG 
     3361 TTCATCAAAG TGTTGTAAAC ATGAAGGAGA TTGTCACAGA CAAACAAGAT GCACTGGATT 
     3421 TCAAGAAGGA CAAAGGTGCC TTTTACCTTG TATTTGAATA TATGGACCAT GACTTAATGG 
     3481 GACTGCTAGA ATCAGGTTTG GTGCACTTTT CTGAGGACCA TATCAAGTCA TTCATGAAAC 
     3541 AGCTAATGGA AGGACTGGAT TACTGTCACA AAAAGAATTT CCTCCATCGG GATATTAAAT 
     3601 GTTCTAACAT TTTGCTGAAT AACAGTGGGC AAATCAAACT GGCAGATTTT GGCCTTGCTC 
     3661 GGCTCTATAA CTCTGAAGAG AGTCGCCCTT ACACAAACAA AGTCATTACT CTGTGGTATC 
     3721 GACCTCCAGA GCTGCTTCTT GGAGAGGAAA GATACACACC AGCCATTGAT GTTTGGAGCT 
     3781 GTGGGTGCAT CCTTGGGGAA CTGTTCACAA AGAAACCTAT TTTTCAAGCC AATTTGGAAC 
     3841 TGGCTCAGCT AGAACTGATC AGTCGTCTCT GTGGTAGTCC TTGTCCAGCA GTGTGGCCTG 
     3901 ATGTTATCAA GCTGCCCTAC TTCAACACCA TGAAACCGAA AAAGCAATAC AGGAGACGCC 
     3961 TAAGAGAAGA ATTCTCTTTC ATTCCTTCAG CGGCACTTGA TCTGTTGGAC CACATGCTGA 
     4021 CACTGGATCC TAGCAAGCGC TGCACAGCGG AACAAACCCT ACAGAGTGAC TTTCTTAAAG 
     4081 ATGTGGAACT CAGCAAAATG GCACCTCCAG ACCTACCTCA CTGGCAGGAT TGCCATGAAT 
     4141 TGTGGAGTAA GAAACGTCGA CGGCAACGAC AAAGTGGTAT TGTGATAGAA GAGCAACCTC 
     4201 CATCCAAAGC TTCTCGAAAA GAAACTACCT CAGGGACAGC AGCTGAGCCT GTGAAGAACA 
     4261 GTAGCCCAGC ACCACCTCAA CCTGCTCCTG TCAAGGCAGA GCCTGGTCCA GGGGATGCAG 
     4321 TAGGGCTTGG TGACATCACA CAGCAGTTGA ATCAAAGTGA ATTGGCAGTG TTATTAAACC 
     4381 TGCTTCAGAG CCAAACTGAC CTGAGCATCC CTCAGATGGC ACAATTGCTT AACATCCACT 
     4441 CCAATCCAGA GATGCAACAG CAGCTTGAAG CCCTGAATCA GTCTATTAGT GCACTGACTG 
     4501 AAGCCAGTTC CCAGCAGCAA GACTCAGAAT CCATAGCCCC AGAGGAATCA TTGAAGGAGG 
     4561 TACCTTCTGT ATCTGTGGTC CTGCCTCCTG CTGAACAGAC AACTCCTGAA GCTTCAAACA 
     4621 CACCAGCTGA CATGCAGAAT ATGTTGGCAG TTCTCTTGAG TCAGCTGATG AAAACCCAAG 
     4681 AGCCAGCAGG TAACCTGGAG GAAAACACCA GTGACAAGAA TAGTGGGCCA CAGGGGCCCC 
     4741 GAAGAACCCC TACAATGCCA CAGGAGGAGG CAGCAGCATG TCCTCCTCAC ATTCTTCCAC 
     4801 CAGAGAAGAG GCCCCCTGAA CCCCCTGGAC CTCCACCGCC GCCACCTCCA CCCCCTCTGG 
     4861 TTGAAGGCGA TCTTTCCAGC GCCCCCCAGG AGTTGAATCC CGCCGTGACA GCCGCCTTGC 
     4921 TGCAACTTTT ATCCCAGCCT GAAGCAGAGC CTCCTGGCCA CCTGCCACAT GAGCACCAGG 
     4981 CCTTGAGACC AATGGAGTAC TCCACTCGAT CCCATCCAAA CAGGACTTAC GGAAATACTG 
     5041 ATGGGCCTGA AACAGGGTTC AGTGCCACTG ACACTGATGA ACGCAGTTCT GGTCCAGCCT 
     5101 TGACAGAATC TTTGGTTCAG ACCCTGGTGA AGAACAGGAC CTTCTCAGGC TCTGTGAGCC 
     5161 ACCTTGGGGA GTCCAACAGT TACCAGGGCA CAGGGTCAGT GCAGTTCCCA GGGGACCAGG 
     5221 ACCTCCGTTT TACTAGGGTT CCCTTAGCAT TACACTCAGT GGTTGGGCAA CCATTCCTGA 
     5281 AGTCTGAGGG AAATAGCAAC TCTGTGGTAC ATGCAGAGAC CAAATTGCAA AACTATGGGG 
     5341 AGCTGGGACC AGGAACCACT GGGGCCAACA GCTCAGGAAC AACCCTTCAG TGGGGGGGCC 
     5401 CAGCTCAGTC TTTTGGAAAA CCCTACCGAG GGGCTGCAAG AGTCCCACCA CGAGGGGGAA 
     5461 GAGGGAGAGG GGTTCCTTAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5521 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5581 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5641 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5701 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5761 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5821 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5881 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5941 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6001 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6061 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6121 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6181 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6241 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6301 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6361 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6421 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6481 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6541 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6601 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6661 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6721 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6781 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6841 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6901 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6961 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7021 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7081 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7141 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7201 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7261 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7321 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7381 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7441 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7501 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7561 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7621 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7681 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7741 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7801 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7861 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7921 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7981 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8041 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8101 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8161 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8221 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8281 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8341 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8401 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8461 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8521 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8581 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8641 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8701 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8761 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8821 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8881 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8941 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9001 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9061 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9121 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9181 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9241 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9301 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//