LOCUS       RR200138                7764 bp    DNA     circular  SYN 
DEFINITION  Usp4 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 1, (10 ug)
ACCESSION   NM_001135012
VERSION     NM_001135012.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4012 
                     /label="Myc-DDK"  
     misc_feature    4042..4627 
                     /label="PolyA signal"  
     misc_feature    4099..4118 
                     /label="Restriction Enzyme"  
     misc_feature    4903..5575 
                     /label="ColE1"  
     misc_feature    6005..6799 
                     /label="Kan/Neo"  
     misc_feature    6834..7136 
                     /label="SV40 ori"  
     misc_feature    7222..7699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="Usp4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC CGGGGCACCC ATGAGCGACC GGATGTGGAG ACCCAGAAGA 
     1081 CGGAGCTCGG AGCCTTGATG GGGACCACGC TCCAGCGTGG GGCTCAGTGG TATCTGATCG 
     1141 ACAGCCGGTG GTTCAAGCAG TGGAAGAAGT ACGTCGGCTT TGACAGCTGG GACATGTACA 
     1201 ACGTGGGGGA GCACAATCTC TTTCCTGGAC CGATTGACAA CTCTGGGCTC TTCTCAGATC 
     1261 CTGAGAGTCA GACCTTGAAG GAACACTTAA TCGATGAGCT GGACTATGTG TTGGTTCCAA 
     1321 CTGAAGCCTG GAATAAATTG CTAAATTGGT ATGGCTGTGT GGAAGGCCAG CAGCCTATTG 
     1381 TCAGAAAAGT TGTGGAGCAT GGCCTGTTTG TCAAGCACTG CAAAGTGGAA GTGTATTTGC 
     1441 TGGAGTTGAA GCTCTGTGAG AACAGTGACC CCACCAATGT GCTAAGTTGC CATTTTAGCA 
     1501 AAGCAGATAC CATTGCAACT ATTGAGAAGG AGATGAGGAA GCTCTTCAAC ATCCCTGCAG 
     1561 AGCGTGAAAC ACGACTTTGG AACAAATACA TGAGCAACAC CTATGAGCAG TTGAGCAAGC 
     1621 TAGACAACAC TATCCAGGAT GCTGGGCTGT ACCAGGGTCA GGTGCTAGTA ATTGAGCCCC 
     1681 AAAATGAAGA CGGCACATGG CCCAGGCAGA CCCTGCAATC AAAATCAAGC ACTGCACCTA 
     1741 GCAGAAATTT TACTACCTCT TCAAAACCAT CCGCAAGTCC CTATTCCTCA ATGTCTGCCT 
     1801 CTCTCATTGC AAATGGTGAT AGCACTAACA GCTCTGGGAT GCACAACTCC GGTGTCAGCA 
     1861 GGGGTGGAGC TGGCTTCTCT GCCTCGTATA ATTGCCAGGA GCCCCCATCA CCTCATATCC 
     1921 AGCCTGGCCT CTGTGGACTC GGAAACCTGG GAAACACCTG TTTTATGAAT TCTGCTTTGC 
     1981 AGTGCCTGAG CAACACTGGT CCACTGACTG AGTACTTTCT CAAAGATGAG TATGAGGCCG 
     2041 AGATCAACAG AGACAACCCT CTGGGGATGA AAGGGGAGAT TGCAGAGGCC TATGCAGAGC 
     2101 TCATCAAGCA GATGTGGTCT GGAAGGGACA CCCACGTAGC ACCACGGATG TTCAAAACGC 
     2161 AAGTGGGACG TTTCGCCCCT CAGTTTTCTG GCTACCAACA ACAAGACTCT CAAGAGCTGT 
     2221 TAGCCTTTAT TCTAGATGGA TTGCATGAAG ACCTGAACCG AGTAAAGAAG AAGCCTTACC 
     2281 TGGAGCCAAA GGATGCCAAT GGGCGACCAG ATGCGGTGGT AGCAAAGGAA GCCTGGGAAA 
     2341 ATCACAGGCT GAGGAATGAC TCTGTGATTG TGGATACTTT CCATGGCCTT TTCAAATCTA 
     2401 CTTTGGTTTG CCCAGAATGT GCTAAAGTTT CTGTGACCTT TGACCCATTT TGCTATCTAA 
     2461 CTCTCCCACT GCCTTTGAAG AAAGATCGAA TTATGGAGGT CTTCCTGGTT CCTGCTGACC 
     2521 CTCATTGCAG ACCTATCCAG TACCGTGTGA CTGTGCCATT GATGGGGGCC ATTTCTGACC 
     2581 TGTGTGAAGC ACTCTCCAAG CTGTCTGGCA TTGCTGCAGA AAATATGGTG GTCACTGATG 
     2641 TGTATAATCA CCGCTTCCAC AAAATTTTCC AAATGGATGA AGGTTTAAGC CACATCACGC 
     2701 CTCGAGATGA CATTTTTGTG TATGAGATCT GCACCACTCC CATGGATGGC TCAGAATATA 
     2761 TCACTCTCCC AGTCTACTTC AGAGAAAAGA AGTCCAGGCC ATCGAGTACT TCCTCTGGGG 
     2821 CCGTGCTCTA TGGACAGCCA CTTCTTGTTT CTGTCCCTAA GCACAGACTA ACCCTCGAGT 
     2881 CCTTATACCA GGCTGTTTGT GAACGTATCA GCCGCTATAT AAAGCAGCCT TTGCCTGAGG 
     2941 AGTTTCTCAG CTCTCCCTTA GAGCCTGGGG CCTGCAATGG CTCTAGGGGT AGCTATGAAG 
     3001 GAGATGAAGA AGAAATGGAT CATCAAGAGG AAGGGAAAGA ACAGCTTTCT GAAGTGGAAG 
     3061 AAAGTGGTGA GGACAGTCAG GGAGGGGACC CTACTGAGAC CACCCAAAAG GCGAAAGGCC 
     3121 CGCCCCGCCA CAAGAGGCTT TTCACCTTCA GCCTCGTGAA CTCCTGTGGA ACTGCTGATA 
     3181 TCAACTCGCT GGCCACTGAC GGAAAACTCC TCAAGCTCAA CTCTCGATCC ACACTGGCCA 
     3241 TTGACTGGGA CAGTGAGACC CGAAGCCTTT ACTTTGATGA GCAAGAATCT GAGGCCTGTG 
     3301 AGAAGCACAC GAGCATGTCC CAGCCTCAGA AGAAGAAGAA GGCTGCAATA GCCCTGCGGG 
     3361 AGTGCATCGA GCTCTTTACT ACCATGGAGA CCCTTGGGGA GCATGACCCC TGGTACTGTC 
     3421 CCACCTGTAA GAAGCACCAA CAGGCAACAA AAAAGTTTGA TTTGTGGTCT TTGCCCAAGA 
     3481 TCCTGGTGGT TCATCTCAAA CGTTTCTCCT ATAACAGATA CTGGCGGGAT AAACTGGACA 
     3541 CAGTTGTGGA ATTCCCAGTA AGAGCTCTGA ACATGTCCGA GTTTGTGTGT GACCGGGCAG 
     3601 CAAGGCCTTA TGTCTATGAC CTGATTGCTG TGTCCAATCA CTATGGCGCT ATGGGGGTCG 
     3661 GTCACTATAC TGCATATGCG AAGAACAGAC TGAATGGGAA ATGGTATTAC TTTGATGATA 
     3721 GCAGCGTGTC CCTGGCCTCT GAGGATCAGA TAGTGACGAA GGCCGCCTAC GTGTTGTTTT 
     3781 ACCAGCGTCG GGATGATGAG TGTCCCAGCA CCTCCTCACC TGTCAGCTTC CCGGGTTCTG 
     3841 ATGGAGGGGC GAAGCTCAGC AGCTCACAGC AGGACTTGGG GGAAGAGGAG GCTTACACCA 
     3901 TGGACACCAA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3961 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4021 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4081 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4141 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4201 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4261 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4321 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4381 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4441 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4501 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4561 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4621 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4681 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5521 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5581 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5641 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5701 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5761 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5821 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5881 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5941 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6001 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6061 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6121 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6181 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6241 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6301 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6361 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6421 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6481 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6541 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6601 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6661 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6721 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6781 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6841 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6901 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6961 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7201 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7321 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7381 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7441 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7501 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7561 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7621 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7681 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7741 TAACAAAAAT TTAACGCGAA TTTT
//