LOCUS       RR200091                8268 bp    DNA     circular  SYN 
DEFINITION  Tacc2 (Myc-DDK-tagged ORF) - Rat transforming, acidic coiled-coil containing protein 2 (Tacc2), transcript variant 2, (10 ug)
ACCESSION   NM_001004418
VERSION     NM_001004418.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4425..4516 
                     /label="Myc-DDK"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    4603..4622 
                     /label="Restriction Enzyme"  
     misc_feature    5407..6079 
                     /label="ColE1"  
     misc_feature    6509..7303 
                     /label="Kan/Neo"  
     misc_feature    7338..7640 
                     /label="SV40 ori"  
     misc_feature    7726..8203 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4407..4413 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4407 
                     /label="Tacc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACGAG AACAGCACCT CGGACCACCA GAGGACTTCT TCAGTTCAGA 
     1081 GTCTAAGATC ACTGCAGCCA CCCGGGAACA GTCAAACTCC ACAGAAGCAA GGAGACTCAC 
     1141 CTGGATCCGG CGCTGCAAGC ATCCCTGGAA CCAATGATGT CATCCAGTCA GCTGCTCCCG 
     1201 AAGACCTGGG ACACCCACCC TTAGCTGATT CTTCCCGCTA TGGTGTTACT GTCAGCTCAG 
     1261 TCTCTACACA TCTGACAGTC CAGAGCACCT CCCCATCTGC TGCCCATGCA AGTCTTGCTC 
     1321 CTGTGGCCTC AGAACATGCA GCCTTGGCTT CCGCCGCAGC TGGTCCTGGA GTAGAAACCC 
     1381 CCACTGCCTC CTGCCAACAC CTGGCCAAGA ACATAAACAG GAGTTCCGAC TCTGAGGAAG 
     1441 CCTTTGAGAC CCCCGAGTCA ACAACCCCTG TCAAAGCTCC ACCAGCTCCT CCCCCTCCAC 
     1501 CCCCTGAAGT CACCCCGGAG CCTGAGGTCA TCGAACCACC AGCCCCAGAA GAACCAGGAT 
     1561 GCATTTCTGA GCCGGTCGTG GTTCCCGATG GCCCTCGCAG CGAGTCCGTG GAAGGAAGCC 
     1621 CTTTCCGTCC TTCGCACTCC TCCTCGGCGG TGTTCGATGA AGACAAGCCG ATAGCCAGCA 
     1681 GCGGGACATA CAACTTAGAC TTTGACAGCA TCGAGCTGGT GGATAACTTT CAGAGTTTGG 
     1741 AGCCATGCTC TGCCGACTAT AAGGGTCAGG AGTGTAAGGT GAGCACGCGG AGGAAATCCA 
     1801 CCGAGTCCGT GCCACCCTCC AAGACCACGC TGTCCCGCTC GCTCAGCCTG CAAGCCAGCG 
     1861 ACTTCGACGG TGCTTCGTGC CCCGGCAGCC CGGAAGCTGG GACCCTTGCC ACAGATGCGT 
     1921 GTGGCACAGG ATCCAACAGT GCTTCTAGCA CCCTTAAGCG AACTAAAAAA ACACGGCCAC 
     1981 CTTCCTTAAA AAAGAAACAA GCTACCAAGA AACCCACAGA AACCCCCCCA GTGAAGGAGA 
     2041 CCCAACAGGA GCCAGGTGAA GAGAGTCCGG TGCCCAGTGA GGAACACTTA GCACCAGAGA 
     2101 CAAAGACAGA ATCGGCCACA CCTGAGGGCA CTGGTTGCAC CTTGTCAGAA GAGACATCTC 
     2161 TGGAGTCTGC TGCTGTGCCC ACAGCTACCT GTCCTCTGAC TTTGGAGAGT GTGGAAGACG 
     2221 TTAGCCCTCT GGTTTCTGGA GGTGGCAGAG TGCAGAACTC ACCCCCTGTG GGGAGGAAAT 
     2281 CAGTGCCTCT TACCACAGCC TCTGAGGCAG TGGAGGTGAC GCTGCCGGAC GGTGGGGGGC 
     2341 AAGAGGACTT GCCTGCCAAA GGGCTGTCAG TGAGGCTGGA GTTTGACTAC TCTGAGGACA 
     2401 AGGGAAGTTG GGAGAGTCAG CAGGAGAACG CCCCTCCCAC CAAAAAGATA GGCAAGAAGC 
     2461 CAGTTGCCAA AATGCCCCTA AGGAGGCCAA AGCTGAAAAA GACCCCAGAG AAACTGGACA 
     2521 ACACTCCTGC CTCACCTCCA AGGTCCCCTG CTGAACCCAG TGACATCCCT ATTGCTAAAG 
     2581 GTACCTACAC CTTTGATATA GACAAGTGGG ATGACCCCAA TTTTAACCCT TTTTCCTCCA 
     2641 CCTCGAAAAT GCAAGAGTCT CCCACACTGT CCCAACAATC GTACAACTTT GACCCCGACG 
     2701 CCTGTGAAGA GTCCCTTGAC CCCTTTAAGG CATCCTCTAA GACCCCCAGT TCACCTTCTA 
     2761 AATCCCCAGC CTCTTTCGAG ATCCCAGCCA GCACCATCGA AGCGGACGGG GATGGGTTGA 
     2821 ATAAACCCGC CAAGAAGAAG AAGACTCCAT TGAAGACTGA CACATTTAGG GTGAAGAAGT 
     2881 CTCCAAAGCG GTCTCCTCTG TCTGATCCGC CTTCTCAGGA CCCCACTCCA GCTGCCACAC 
     2941 CGGAAGCACC TCCAGTCTCT ACCGTGGTCC ACGCCACAGA TGAGGAAAAG TTGGCCGTCA 
     3001 CTAGCCAGAA GTGGACATGT ATGACAGTGG ACTTGGATGC TGACAAACAG GATTTCCCGC 
     3061 AGCCCTCAGA CCTATCTAAC TTTGTAAATG AGACCAAATT CAATTCACCC TCGGAGGAGC 
     3121 TGGACTACAG AAACTCCTAT GAAATTGAAT ACATGGAAAA GCTTGGCTCC TCCTTACCTC 
     3181 AGGACGATGA CACTCCGAAG AAGCAGGCCT TGTACCTTAT GTTTGACACC CCTCAGGAGA 
     3241 GCCCCGTCAA GTCTCCTCCG GTCCGGATGT CGGATTCCCC AACTCCATGT TCAGGGTCGA 
     3301 GTTTTGAGGA CACCGAAGCC CTGGTGAATG CAACAGCAAA GCTCCAGCAT CCAGTCCCTA 
     3361 GAGGGCTGGC CTCCAATCAG GAGCCTCTCT TGCAGCTGCC GGAAAAATCC TCCCAGAAGG 
     3421 AGCTGGAGGC CATGGCCTTG GGCACCCCCG CAGAAGCAAT TGAAATCACA GCTCCAGAGG 
     3481 GTGCCTTTGC CTCTGCAGAT GCCCTCCTCA GCAGGCTGGC CCATCCTGCT TCTCTCTGTG 
     3541 GTGCACTTGG CTATCTGGAG CCTGATTTAG CAGAAAAGAA CCCCCCAGTA TTTGCCCAGA 
     3601 AACTTCAGGA GGAATTAGAG TTTGCTGTCA TGCGGATAGA AGCCTTGAAG CTGGCCAGGC 
     3661 AGATCGCCCT GGCTTCTCGC AGCCGCCAGG ACACCAAGAG GGAAGCCACT CACCCACCAG 
     3721 ATGTCTCCAT CTCCAAAACT ACCTTGTACT CCCGCATCGG GCCCACCGAG GTGGAGAAAC 
     3781 CCCCAGGCCT TCTGTTCCAG CAGCCAGACC TGGACTCTGC ACTCCAAGTT GCCAGAGCAG 
     3841 AGGTCATCGC CAAGGAGAGA GAAGTCTCAG AGTGGAGGGA TAAATATGAA GAAAGCCGGC 
     3901 GGGAAGTGGT AGAAATGAGG AAAATCGTGG CTGAATACGA GAAGACCATC GCACAGATGA 
     3961 TCGAGGATGA ACAGAGAGAG AAGTCCATCT CCCACCAAAC CGTGCAGCAG CTGGTGCTGG 
     4021 AGAAGGAGCA AGCCCTGGCT GACCTGAACT CTGTGGAGAA GTCTCTGGCT GACCTCTTCA 
     4081 GGCGATACGA GAAGATGAAG GAGGTGCTGG AAGGCTTTCG AAAGAATGAG GAGGTGCTGA 
     4141 AGAAGTGTGC ACAGGAGTAC CTGTCCCGCG TGAAGAAAGA GGAGCAGAGG TACCAGGCCC 
     4201 TGAAGGTGCA TGCAGAAGAG AAACTGGACA GAGCCAATGC AGAGATTGCC CAGGTTCGAG 
     4261 GCAAGGCCCA GCAGGAGCAG GCGGCCTACC AGGCTAGCCT GAGGAAGGAG CAGCTTCGAG 
     4321 TGGATGCTCT AGAAAGAACG CTGGAGCAGA AGAATAAAGA GATAGAAGAA CTCACCAAGA 
     4381 TTTGTGACGA ACTGATTGCC AAAATGGGGA AAAGCACGCG TACGCGGCCG CTCGAGCAGA 
     4441 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4501 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5221 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5281 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5341 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5401 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5461 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5521 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5581 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5641 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5701 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5761 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5821 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5881 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5941 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6001 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6061 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6121 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6181 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6241 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6301 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6361 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6421 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6481 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6541 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6601 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6661 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6721 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6781 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6841 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6901 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6961 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7021 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7081 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7141 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7201 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7261 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7321 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7381 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7441 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7501 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7681 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7741 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7801 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7861 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7921 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7981 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8041 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8101 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8161 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8221 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//