LOCUS       RR200027                8031 bp    DNA     circular  SYN 
DEFINITION  Tsc22d1 (Myc-DDK-tagged ORF) - Rat TSC22 domain family, member 1 (Tsc22d1), transcript variant 1, (10 ug)
ACCESSION   NM_001109912
VERSION     NM_001109912.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4188..4279 
                     /label="Myc-DDK"  
     misc_feature    4309..4894 
                     /label="PolyA signal"  
     misc_feature    4366..4385 
                     /label="Restriction Enzyme"  
     misc_feature    5170..5842 
                     /label="ColE1"  
     misc_feature    6272..7066 
                     /label="Kan/Neo"  
     misc_feature    7101..7403 
                     /label="SV40 ori"  
     misc_feature    7489..7966 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4170..4176 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4170 
                     /label="Tsc22d1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAGCCG CCAGAGTCCA CCGCCGCGGC GGCCGCTGCT GCAGACATTA 
     1081 GTGCTAGGAA GATGGCGCAC CCGGCAATGT TCCCTCGAAG GGGCAGCGGT GGTGGCAGCG 
     1141 CCTCTGCTCT CAGTGCAGCA GGTACCGGCG TCAGTGGTGC TGCTCCATCT TCTGAAGATT 
     1201 TTCCTCCTCC TTCTTCGCTG CTCCAGCCGC CGCCTCCTGC TGCATCTTCC ACACAGGGAC 
     1261 CACAGCCTCC GCCTCCACAA AGCCTGAACC TCCTTTCACA GGCTCAGCTG CAGGGACAGC 
     1321 CTCTTGCGCC AGGCGGAACT CAGATGAAAA AGAAAAGTGG CTTCCAGATA ACTAGCGTTA 
     1381 CTCCGGCTCA GATCTCTGCC AGCATCAGCT CGAACAACAG CATAGCAGAG GACACCGAGA 
     1441 GCTATGATGA CCTGGATGAG TCGCACACGG AAGATCTCTC TTCTTCCGAG ATCCTTGATG 
     1501 TCTCCCTTTC CAGGGCCACT GACTTAGGTG AGCCTGAACG CAGTTCCTCC GAGGAGACTC 
     1561 TGAATAACTT CCAGGAAGCT GAGACACCTG GGGCAGTCTC TCCTAACCAG CCCCACCTTC 
     1621 CTCAGCCTCA TTTGCCTCAC CTTCCACAAC AGAACGTTGT GATCAATGGG AACGCTCATC 
     1681 CACACCACCT CCATCACCAC CATCACATTC ATCATGGGCA CCACCTTCAC CACGGACACC 
     1741 ACCATTCATC ACATGCTGCT GTGGCCAGTA CATCCGTTCC TGGAGGGCCA CCACCCACGA 
     1801 GCCCCGTGTC CAGGAAACTC TCTACAGCTG GAAGTTCTGA CGGTGGTGTG CCAGTCCCAC 
     1861 CTACTTCTGC TGTATCATCG GCTGGCTTAC CTGCCTCAGT GATGACGAGC ATCCGTGCTC 
     1921 CAAGCACTCC AGGCAGCATA GGTGTAAATT CAGTTACCGG CACGAATGCA ACGAATAATG 
     1981 TTAACATTGC TGCTGTGGGT GGTTTCAGTC CCGGTGTGGC GAACAGCGTG CATCCCAATG 
     2041 CTGTTAGTAT AAGTGGTGGG CCTGGAGTTA CCAGCGGTGT TAATGTGAAT GTCTTGAGTG 
     2101 GCATGGGCAA CGGTACCATT TCTTCTCCTG TCCTTAACGC AGCTGCAGGT ATCACGGTGG 
     2161 GAGTGGTTTC AAGTCAGCAG CAGCAGCCGC AGCAGCCGCC GCCAACAGTT AACACATCAA 
     2221 GGTTCAGAGT TGTGAAGTTA GACTCTACTT CTGAACCCTT TAAAAAAGGC AGATGGACTT 
     2281 GCACTGAATT CTATGAGAGA GAAAATGCTG TGCCAGGTCC AGAAGGCGTG GCCATAAATA 
     2341 AAGTGGTGGA GACCGTGAAG CAAACCCCCA CGGAAGCGTC CTCTTCGGAG AGGGAGAGCA 
     2401 CTAGTGGGAG TTCCGTGAGC AGTAGTGTCA GCACACTGAG TCACTACACA GAGAGTGTGG 
     2461 GAAGCGGAGA GATGGGAGCC CCTACTGTGG TGGTGCAGCA GCAGCAGCCG CTACCACCAG 
     2521 CACCACCAGG TCTTCAAGGC GTGGCCCTCC AACAGCTAGA TTTCAGTAGC CCTGGTCCAC 
     2581 AGAGTATTTC TCAGTCACAG ATGTCACAGG TACAACTACA GCCTCAAGAA TTGAGCTTTC 
     2641 AGCAGAAGCC AAATCTTCAA CCTGTTCCTC TGCAAGCCAC TATGAGCGCA GCAACTGGTA 
     2701 TCCAGCCTTC ACCTGTCAAC GTGGTGGGTG TAACGTCAGC TGTAGGTCAA CAGCCTTCCA 
     2761 TTTCCAGTCT GGCTCAGCCC CAGCTGCCGT ATCCTCAGCC AGCTCCTCCC GTGCAAACTC 
     2821 CTCTTCCAGG GGCACCACCC CAGCAGTTAC AATATGGGCA ACAGCAACCA ATGGTTCCTA 
     2881 CGCAGATAGC CCCAGGCCAT GGCCAGCCAG CAACTCAAAA CCCTCCTTCA GAGTACGCAC 
     2941 AGCAGCAGCC AATATTCCAA GCGGCAATGT CCTCTGGACA GTCCAGTTCC ACAGGCACAG 
     3001 GAGCGTCAGT GATTCCCGTG GCTCAGCCAC AAGGGATCCA GCTGCCAGTG CAGCCCACAG 
     3061 CAGTACAAGC ACAACCTGCA GGGGCGACTG GGCAGCCCAT TGGCCAGGCT CCAACAGCAG 
     3121 TGTCTACTGT ACCAGCTGGT GGTCAGATTG CAAGTATCGG TCAGCAGGCA AACATACCCA 
     3181 CTGCAGTACA GCCACCCTCT ACCCAAGTTA CACCTTCAGT TGTTCAGCAA GGTGCTCCTC 
     3241 CATCTTCACA AGTAGTCCTA CCTGCTCCAA CTGGGATCAT TCATCAGGGC GTTCAAACCA 
     3301 GTGCTCCAAG CCTTCCTCAG CAATTGGTTA TTGCACCCCA GAGTACCATG GTAGCGGTGC 
     3361 CTCCCCAGAC ACAGGGAGTG GAAGCAGTGG CTCATGGAGT TGTTTCCCAC CAGTTGCCCA 
     3421 CAGGTAGTCC TTTGCCCTCT GCTAGCACCA TTTCTGTTAC AAGTCAGGTT AGTTCAGCTG 
     3481 GTCCTTCTGG AATGTCTTCT GTCCCAACAA ACTTAGTTCC ACCACAGAAT ATAGCACAAC 
     3541 CCCCAGCCAC CCAAAATGGC AGTTTGGTTC AAAGTGTTAG TCAATCTCCA TTGATAGCCA 
     3601 CTAACATAAA TTTGCCTTTG GCACAACAGA TACCGCTAAG TTCTACTCAG TTCTCTACAC 
     3661 AATCATTAGC TCAGGCAATT GGAAGCCAAA TGGAAGACAC CAGGCGTCCA GCGGAGCCCT 
     3721 CCTCAGGCGG CTTACCTCAG ACTCTCAGTG GTGACAGTGG GGGAGTGTCA GCAGTCTCAG 
     3781 ATGGGAGTAG CAGCAGCCTA GCAGCCTCTG CTTCTCTCTT CCCGTTGAAG GTGCTACCGC 
     3841 TGACAACACC CCTGGTGGAT GGCGAGGACG AGAGCTCTGG TGCAAGTGTG GTAGCTATCG 
     3901 ACAACAAAAT AGAGCAAGCT ATGGATCTGG TGAAAAGCCA TTTGATGTAT GCAGTTAGAG 
     3961 AGGAAGTGGA GGTTCTGAAG GAGCAGATCA AAGAACTAAT AGAGAAAAAC TCCCAACTGG 
     4021 AGCAGGAGAA CAATCTGTTG AAGACACTGG CCAGTCCGGA GCAGCTCGCC CAGTTTCAGG 
     4081 CCCAGCTGCA GACTGGCTCC CCTCCGGCTA CCACGCAGCC ACAGGGGACC ACACAGCCCC 
     4141 CTGCACAGCC AGCGTCCCAG GGCTCAGGAT CAACCGCAAC GCGTACGCGG CCGCTCGAGC 
     4201 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4261 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4321 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4381 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4441 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4501 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4561 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4621 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4681 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4741 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4801 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4861 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4921 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4981 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5041 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5101 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5161 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5221 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5281 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5341 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5401 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5461 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5521 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5581 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5641 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5701 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5761 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5821 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5881 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5941 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6001 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6061 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6121 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6181 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6241 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6301 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6361 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6421 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6481 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6541 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6601 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6661 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6721 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6781 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6841 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6901 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6961 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7021 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7081 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7141 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7201 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7261 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7321 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7381 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7441 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7501 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7561 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7621 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7681 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7741 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7801 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7861 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7921 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7981 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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