LOCUS RN202906 8121 bp DNA circular SYN DEFINITION Casr (untagged ORF) - Rat calcium-sensing receptor (Casr), (10 ug) ACCESSION NM_016996 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4278..4369 /label="Myc-DDK" misc_feature 4399..4984 /label="PolyA signal" misc_feature 4456..4475 /label="Restriction Enzyme" misc_feature 5260..5932 /label="ColE1" misc_feature 6362..7156 /label="Kan/Neo" misc_feature 7191..7493 /label="SV40 ori" misc_feature 7579..8056 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4260..4266 /label="Restriction Enzyme" misc_feature 1020..4260 /label="Casr" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCATCGTAC AGCTGCTGTT TGGCCCTCTT GGCTCTTGCC TGGCACTCCT 1081 CTGCCTATGG GCCTGACCAG CGAGCCCAAA AGAAGGGGGA CATTATCCTA GGAGGTCTCT 1141 TTCCTATCCA TTTTGGAGTA GCAGCCAAAG ATCAAGATCT GAAGTCAAGA CCAGAGTCTG 1201 TGGAGTGCAT TAGGTATAAC TTCCGTGGAT TCCGATGGTT ACAAGCCATG ATATTCGCCA 1261 TAGAGGAGAT AAACAGCAGC CCCTCCCTTC TTCCCAACAT GACACTGGGA TATAGGATAT 1321 TTGACACCTG TAACACCGTC TCCAAGGCGC TGGAAGCCAC CTTGAGTTTT GTTGCCCAGA 1381 ACAAAATCGA TTCTTTGAAC CTGGACGAGT TCTGCAACTG CTCTGAGCAC ATCCCTTCGA 1441 CCATTGCCGT GGTGGGAGCC ACCGGCTCCG GTGTCTCCAC GGCGGTAGCC AACCTGCTGG 1501 GACTTTTCTA CATCCCCCAG GTGAGCTACG CCTCCTCCAG CAGGCTCCTC AGCAATAAGA 1561 ACCAGTACAA ATCCTTCCTC CGCACCATTC CCAATGACGA ACACCAGGCA ACCGCGATGG 1621 CCGACATCAT CGAGTACTTC CGCTGGAACT GGGTGGGCAC AATTGCAGCT GATGACGACT 1681 ATGGCAGACC TGGCATTGAG AAGTTCCGAG AGGAAGCCGA AGAGAGGGAT ATCTGCATTG 1741 ATTTTAGCGA GCTCATCTCC CAGTACTCTG ACGAGGAAGA GATCCAGCAG GTGGTCGAAG 1801 TGATCCAAAA CTCTACGGCC AAGGTCATTG TCGTTTTCTC CAGCGGCCCG GACCTAGAAC 1861 CTCTCATCAA GGAGATTGTG CGGCGTAACA TCACAGGCAG GATCTGGCTG GCTAGCGAGG 1921 CCTGGGCCAG TTCCTCGCTG ATTGCTATGC CTGAGTATTT CCATGTAGTC GGGGGCACCA 1981 TTGGGTTCGG TCTGAAGGCT GGGCAGATTC CAGGCTTCCG AGAATTCCTA CAGAAAGTTC 2041 ATCCTAGGAA GTCTGTCCAC AATGGTTTTG CCAAAGAGTT TTGGGAAGAA ACTTTTAATT 2101 GCCACCTCCA AGAAGGCGCA AAAGGACCTT TACCTGTGGA CACCTTCGTG AGAAGTCACG 2161 AAGAAGGTGG CAACAGGTTA CTCAATAGCT CTACTGCCTT CCGACCCCTC TGCACAGGGG 2221 ATGAGAACAT CAACAGTGTG GAGACCCCTT ACATGGATTA CGAACATTTA CGGATATCCT 2281 ACAATGTGTA CTTAGCCGTC TACTCCATTG CGCATGCCCT ACAAGATATA TACACCTGCT 2341 TACCCGGAAG AGGGCTTTTC ACCAACGGGT CCTGTGCAGA CATCAAGAAG GTTGAGGCCT 2401 GGCAGGTCTT GAAGCACCTA CGGCACCTGA ACTTCACCAA CAACATGGGG GAGCAGGTGA 2461 CCTTCGATGA GTGTGGTGAT CTGGTGGGGA ACTATTCTAT CATCAACTGG CACCTCTCCC 2521 CAGAGGACGG CTCCATTGTG TTCAAGGAAG TTGGGTACTA CAATGTGTAT GCCAAGAAGG 2581 GAGAAAGACT CTTCATCAAT GAGGAGAAGA TCTTGTGGAG TGGGTTCTCC AGAGAGGTGC 2641 CTTTCTCCAA TTGCAGCCGG GACTGTCAGG CAGGGACCAG GAAGGGGATC ATCGAGGGAG 2701 AGCCCACCTG CTGCTTTGAG TGTGTGGAGT GTCCTGATGG AGAGTACAGT GGAGAGACAG 2761 ATGCGAGTGC CTGTGACAAG TGCCCGGATG ACTTCTGGTC CAATGAGAAC CACACTTCTT 2821 GCATTGCCAA GGAGATTGAG TTTCTGGCGT GGACCGAGCC CTTTGGAATC GCTCTCACTC 2881 TCTTTGCGGT GCTGGGCATT TTCCTGACCG CCTTTGTGCT GGGTGTCTTC ATCAAGTTCC 2941 GAAACACACC TATCGTCAAG GCCACCAACC GAGAACTGTC CTACCTCCTG CTCTTCTCCC 3001 TACTCTGCTG CTTCTCCAGC TCCTTGTTCT TCATTGGGGA GCCCCAGGAC TGGACGTGCC 3061 GCCTGCGACA GCCTGCTTTC GGCATCAGCT TTGTGCTCTG TATCTCGTGC ATCTTGGTGA 3121 AGACCAATCG CGTCCTCCTG GTATTTGAAG CCAAGATACC CACCAGCTTC CACCGGAAGT 3181 GGTGGGGGCT CAACCTGCAG TTCCTGCTGG TTTTCCTCTG CACCTTCATG CAGATCCTCA 3241 TCTGCATCAT CTGGCTCTAC ACGGCGCCCC CCTCTAGCTA CCGCAACCAT GAGCTGGAAG 3301 ACGAAATCAT CTTCATCACG TGCCATGAGG GCTCACTCAT GGCACTTGGC TCCCTGATCG 3361 GCTATACCTG CCTGCTGGCT GCCATCTGCT TCTTCTTTGC CTTCAAGTCC AGGAAGTTAC 3421 CAGAGAACTT CAACGAAGCC AAGTTCATTA CCTTCAGCAT GCTCATCTTC TTCATCGTCT 3481 GGATCTCCTT CATTCCAGCC TATGCCAGCA CCTACGGCAA GTTTGTCTCT GCCGTAGAGG 3541 TGATCGCCAT TTTGGCAGCC AGCTTTGGCT TACTAGCCTG CATCTTCTTC AACAAGGTCT 3601 ACATTATCCT CTTCAAGCCT TCCCGGAACA CCATTGAGGA AGTCCGCTCC AGCACCGCAG 3661 CACATGCTTT CAAAGTAGCA GCCCGCGCCA CTCTACGCCG TCCCAACATC TCCCGGAAGC 3721 GGTCCAGCAG CCTTGGAGGC TCCACCGGCT CCATTCCCTC CTCCTCCATC AGCAGCAAAA 3781 GCAACAGCGA AGACCGGTTC CCGCAGCCAG AGAGGCAGAA GCAACAGCAA CCGCTGTCCC 3841 TGACCCAGCA AGAACAGCAG CAGCAGCCCC TGACCCTCCA CCCACAGCAA CAGCAGCAGC 3901 CACAGCAGCC GAGATGCAAA CAGAAGGTCA TCTTCGGCAG TGGTACGGTC ACCTTCTCTC 3961 TGAGTTTTGA CGAGCCTCAG AAGAATGCCA TGGCCCACAG GAACTCCATG CGTCAGAACT 4021 CCCTGGAGGC CCAGAGGAGC AACGACACCT TGGGCAGACA CCAGGCCCTG CTTCCCCTAC 4081 AGTGTGCAGA TGCGGACTCA GAAATGACCA TTCAGGAAAC GGGCCTGCAA GGGCCCATGG 4141 TGGGGGACCA CCAGCCAGAA ATGGAAAGCT CAGATGAAAT GTCCCCAGCG CTGGTCATGT 4201 CCACCTCTCG GAGCTTCGTC ATTAGTGGTG GAGGTAGCTC TGTGACGGAA AACGTATTAC 4261 ACTCCTAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 4321 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 4381 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 4441 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 4501 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 4561 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 4621 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 4681 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 4741 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 4801 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 4861 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 4921 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 4981 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 5041 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 5101 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 5161 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 5221 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 5281 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 5341 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 5401 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 5461 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 5521 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 5581 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 5641 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 5701 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 5761 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 5821 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 5881 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 5941 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 6001 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 6061 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 6121 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 6181 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 6241 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 6301 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 6361 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 6421 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 6481 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 6541 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 6601 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 6661 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 6721 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 6781 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 6841 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 6901 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 6961 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 7021 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 7081 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 7141 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 7201 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 7261 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 7321 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 7441 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 7501 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 7561 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 7621 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 7681 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 7741 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 7801 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 7861 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 7921 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 7981 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 8041 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 8101 CAAAAATTTA ACGCGAATTT T //