LOCUS       RG239874                9572 bp    DNA     circular  SYN 
DEFINITION  SORBS1 (tGFP-tagged) - Human sorbin and SH3 domain containing 1 (SORBS1), transcript variant 10
ACCESSION   NM_001290296
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4053..4757 
                     /label="GFP"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    7481..8126 
                     /label="ColE1"  
     misc_feature    5843..6637 
                     /label="Neo"  
     misc_feature    5474..5776 
                     /label="SV40 ori"  
     misc_feature    8141..9001 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4032..4038 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4032 
                     /label="SORBS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTGAA TGTGATGGTG GTTCCAAAGC TGTGATGAAT GGCTTGGCAC 
     1081 CTGGCAGCAA TGGGCAAGAC AAAGCAACTG CCGACCCTTT ACGCGCACGC TCTATTTCTG 
     1141 CTGTTAAAAT CATTCCTGTG AAGACAGTGA AAAACGCCTC AGGCCTAGTT CTCCCTACAG 
     1201 ACATGGATCC TACAAAAATC TGCACTGGGA AGGGAGCGGT GACTCTCCGG GCCTCGTCTT 
     1261 CCTACAGGGA AACCCCAAGC AGTAGCCCTG CGAGCCCTCA GGAAACCCGG CAACACGAAA 
     1321 GCAAACCAGA TGAGTGGAGG CTTTCTTCCA GTGCTGATGC CAATGGAAAT GCCCAGCCCT 
     1381 CTTCACTCGC TGCCAAGGGC TACAGAAGTG TGCATCCCAA CCTTCCTTCT GACAAGTCCC 
     1441 AGGATGCCAC TTCCTCCAGT GCAGCCCAGC CGGAGGTAAT AGTTGTCCCT CTCTACCTGG 
     1501 TTAATACTGA CAGAGGGCAA GAAGGCACTG CCAGACCTCC AACACCTCTG GGGCCTCTTG 
     1561 GCTGCGTCCC CACAATCCCA GCGACTGCCT CTGCCGCCTC ACCTCTGACC TTCCCGACTC 
     1621 TAGATGATTT CATTCCCCCT CATCTGCAGA GGTGGCCCCA CCACAGCCAG CCAGCCCGCG 
     1681 CCTCTGGCTC CTTTGCCCCC ATTAGCCAGA CGCCACCATC CTTCTCACCA CCACCTCCGC 
     1741 TGGTCCCTCC TGCCCCGGAG GACCTCCGCA GAGTCTCGGA GCCTGACCTC ACGGGAGCTG 
     1801 TTTCGAGTAC CGATTCCAGT CCTCTACTAA ATGAAGTTTC TTCTTCCCTT ATTGGAACTG 
     1861 ATTCCCAAGC CTTTCCATCA GTTAGCAAGC CTTCATCCGC CTATCCCTCC ACAACGATTG 
     1921 TCAATCCTAC TATTGTGCTC TTGCAACACA ATCGAGAACA GCAAAAACGA CTCAGTAGCC 
     1981 TTTCAGATCC TGTCTCAGAA AGAAGAGTGG GAGAGCAGGA CTCAGCACCA ACCCAGGAAA 
     2041 AACCCACCTC ACCTGGCAAG GCTATTGAAA AAAGAGCAAA GGATGACAGT AGGCGGGTGG 
     2101 TGAAGAGCAC TCAGGACTTA AGCGATGTTT CCATGGATGA AGTGGGCATC CCACTCCGGA 
     2161 ACACTGAGAG ATCAAAAGAC TGGTACAAGA CTATGTTTAA ACAGATCCAC AAACTGAACA 
     2221 GAGACACTCC TGAAGAAAAC CCTTATTTCC CTACGTACAA ATTCCCTGAA CTTCCTGAAA 
     2281 TCCAGCAAAC TTCCGAAGAT GATGATTCAG ATCTGTACTC TCCCAGATAC TCATTTTCTG 
     2341 AAGACACAAA ATCTCCCCTT TCTGTGCCTC GCTCAAAAAG TGAGATGAGC TACATTGATG 
     2401 GTGAGAAGGT AGTCAAGAGG TCGGCCACAC TACCCCTCCC AGCCCGCTCT TCCTCACTGA 
     2461 AGTCAAGCTC AGAAAGAAAT GACTGGGAAC CCCCAGATAA GAAAGTAGAC ACAAGAAAAT 
     2521 ATCGTGCAGA GCCCAAGAGC ATTTACGAAT ATCAGCCTGG CAAGTCTTCC GTTCTGACCA 
     2581 ACGAAAAGAT GAGTCGGGAT ATAAGCCCAG AAGAGATAGA TTTAAAGAAT GAACCTTGGT 
     2641 ATAAATTCTT TTCGGAATTG GAGTTTGGGA AACCGCCTCC CAAAAAGATA TGGGATTATA 
     2701 CTCCTGGAGA CTGCTCTATC CTTCCTAGAG AGGATAGAAA GACTAATCTA GACAAAGATC 
     2761 TCAGCCTCTG CCAGACAGAG TTAGAGGCAG ATTTAGAAAA AATGGAGACG CTTAATAAAG 
     2821 CACCCAGTGC AAACGTGCCA CAGAGCTCAG CCATCAGCCC TACTCCGGAA ATTTCTTCAG 
     2881 AGACTCCTGG ATATATATAT TCTTCCAACT TCCATGCAGT GAAGAGGGAA TCAGACGGGG 
     2941 CTCCTGGGGA TCTCACTAGC TTGGAGAATG AGAGACAAAT TTATAAAAGT GTCTTGGAAG 
     3001 GTGGTGACAT CCCTCTTCAG GGCCTGAGTG GGCTCAAGCG ACCATCCAGC TCTGCTTCCA 
     3061 CTAAAGATTC AGAATCGCCA AGACATTTTA TACCAGCTGA TTACTTGGAA TCCACGGAAG 
     3121 AATTTATTCG AAGACGTCAT GATGATAAAG AGAAACTTTT AGCGGACCAG AGACGACTTA 
     3181 AACGCGAGCA AGAAGAGGCT GATATTGCAG CTCGACGCCA CACAGGCGTC ATTCCGACGC 
     3241 ACCATCAGTT TATCACTAAT GAGCGCTTTG GGGACCTCCT CAATATAGAC GATACTGCAA 
     3301 AAAGGAAATC TGGGTCAGAG ATGAGACCTG CCAGAGCCAA ATTTGACTTT AAAGCTCAGA 
     3361 CACTAAAGGA GCTTCCTCTG CAGAAGGGAG ATATTGTTTA CATTTATAAG CAAATTGATC 
     3421 AGAACTGGTA TGAAGGAGAA CACCACGGCC GGGTGGGAAT CTTCCCACGC ACCTACATCG 
     3481 AGCTTCTTCC TCCTGCTGAG AAGGCACAGC CCAAAAAGTT GACACCAGTG CAGGTTTTGG 
     3541 AATATGGAGA AGCTATTGCT AAGTTTAACT TTAATGGTGA TACACAAGTA GAAATGTCCT 
     3601 TCAGAAAGGG TGAGAGGATC ACACTGCTCC GGCAGGTAGA TGAGAACTGG TACGAAGGGA 
     3661 GGATCCCGGG GACATCCCGA CAAGGCATCT TCCCCATCAC CTACGTGGAT GTGATCAAGC 
     3721 GACCACTGGT GAAAAACCCT GTGGATTACA TGGACCTGCC TTTCTCCTCC TCCCCAAGTC 
     3781 GCAGTGCCAC TGCAAGCCCA CAGCAACCTC AAGCCCAGCA GCGAAGAGTC ACCCCCGACA 
     3841 GGAGTCAAAC CTCACAAGAT TTATTTAGCT ATCAAGCATT ATATAGCTAT ATACCACAGA 
     3901 ATGATGATGA GTTGGAACTC CGCGATGGAG ATATCGTTGA TGTCATGGAA AAATGTGACG 
     3961 ATGGATGGTT TGTTGGTACT TCAAGAAGGA CAAAGCAGTT TGGTACTTTT CCAGGCAACT 
     4021 ATGTAAAACC TTTGTATCTA ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     4081 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     4141 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     4201 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     4261 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4321 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4381 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4441 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4501 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4561 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4621 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4681 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4741 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5461 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5521 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5581 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5641 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5701 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5761 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5821 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5881 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5941 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     6001 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     6061 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     6121 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     6181 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     6241 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6301 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6361 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6421 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6481 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6541 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6601 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6661 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6721 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6781 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6841 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6901 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6961 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     7021 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     7081 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     7141 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     7201 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     7261 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7321 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7381 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7441 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7501 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7561 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7621 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7681 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7741 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7801 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7861 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7921 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7981 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     8041 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     8101 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     8161 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     8221 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     8281 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8341 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8401 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8461 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8521 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8581 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8641 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8701 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8761 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8821 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8881 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8941 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     9001 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     9061 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     9121 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     9181 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     9241 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9301 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9361 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9421 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9481 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9541 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//