LOCUS       RG239860                9536 bp    DNA     circular  SYN 
DEFINITION  SCUBE3 (tGFP-tagged) - Human signal peptide, CUB domain, EGF-like 3 (SCUBE3), transcript variant 2
ACCESSION   NM_001303136
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4017..4721 
                     /label="GFP"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    7445..8090 
                     /label="ColE1"  
     misc_feature    5807..6601 
                     /label="Neo"  
     misc_feature    5438..5740 
                     /label="SV40 ori"  
     misc_feature    8105..8965 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3996..4002 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3996 
                     /label="SCUBE3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTCGGGG CGCGTACCCG GGCTCTGCCT GCTTGTCCTG CTGGTCCACG 
     1081 CCCGCGCCGC CCAGTACAGC AAAGCCGCGC AAGATGTGGA TGAGTGTGTG GAGGGGACTG 
     1141 ACAACTGCCA CATCGATGCT ATCTGCCAGA ACACCCCGAG GTCATACAAG TGCATCTGCA 
     1201 AGTCTGGCTA CACAGGGGAC GGCAAACACT GCAAAGACGT GGATGAGTGC GAGCGAGAGG 
     1261 ATAATGCAGG TTGTGTGCAT GACTGTGTCA ACATCCCTGG CAATTACCGG TGTACCTGCT 
     1321 ATGATGGATT CCACCTGGCA CATGACGGAC ACAACTGTCT GGATGTGGAC GAGTGTGCCG 
     1381 AGGGCAACGG CGGCTGTCAG CAGAGCTGTG TCAACATGAT GGGCAGCTAT GAGTGCCACT 
     1441 GCCGGGAAGG CTTCTTCCTC AGCGACAACC AGCATACCTG TATCCAGCGG CCAGAAGGAA 
     1501 TGAATTGCAT GAACAAGAAC CACGGCTGTG CCCACATTTG CCGGGAGACA CCCAAGGGGG 
     1561 GTATTGCCTG TGAATGCCGT CCTGGCTTTG AGCTTACCAA GAACCAACGG GACTGTAAAT 
     1621 TGACATGCAA CTATGGTAAC GGCGGCTGCC AGCACACGTG TGATGACACA GAGCAGGGTC 
     1681 CCCGGTGCGG CTGCCATATC AAGTTTGTGC TCCATACCGA CGGGAAGACA TGCATCGAGA 
     1741 CCTGTGCTGT CAACAACGGG GGCTGTGACA GTAAGTGCCA TGATGCAGCG ACTGGTGTCC 
     1801 ACTGCACCTG CCCTGTGGGC TTCATGCTGC AGCCAGACAG GAAGACGTGC AAAGATATAG 
     1861 ATGAGTGCCG CTTAAACAAC GGGGGCTGTG ACCATATTTG CCGCAACACA GTGGGCAGCT 
     1921 TCGAATGCAG TTGCAAGAAA GGCTATAAGC TTCTCATCAA TGAGAGGAAC TGCCAGGATA 
     1981 TAGACGAGTG TTCCTTTGAT CGAACCTGTG ACCACATATG TGTCAACACA CCAGGAAGCT 
     2041 TCCAGTGTCT CTGCCATCGT GGCTACCTGT TGTATGGTAT CACCCACTGT GGGGATGTGG 
     2101 ATGAATGCAG CATCAACCGG GGAGGTTGCC GCTTTGGCTG CATCAACACT CCTGGCAGCT 
     2161 ACCAGTGTAC CTGCCCAGCA GGCCAGGGTC GGCTGCACTG GAATGGCAAA GATTGCACAG 
     2221 AGCCACTGAA GTGTCAGGGC AGTCCTGGGG CCTCGAAAGC CATGCTCAGC TGCAACCGGT 
     2281 CTGGCAAGAA GGACACCTGT GCCCTGACCT GTCCCTCCAG GGCCCGATTT TTGCCAGAGT 
     2341 CTGAGAATGG CTTCACGGTG AGCTGTGGGA CCCCCAGCCC CAGGGCTGCT CCAGCCCGAG 
     2401 CTGGCCACAA TGGGAACAGC ACCAACTCCA ACCACTGCCA TGAGGCTGCA GTGCTGTCCA 
     2461 TTAAACAACG GGCCTCCTTC AAGATCAAGG ATGCCAAATG CCGTTTGCAC CTGCGAAACA 
     2521 AAGGCAAAAC AGAGGAGGCT GGCAGAATCA CAGGGCCAGG TGGTGCCCCC TGCTCTGAAT 
     2581 GCCAGGTCAC CTTCATCCAC CTTAAGTGTG ACTCCTCTCG GAAGGGCAAG GGCCGACGGG 
     2641 CCCGGACCCC TCCAGGCAAA GAGGTCACAA GGCTCACCCT GGAACTGGAG GCAGAGGTCA 
     2701 GAGCCGAAGA AACCACAGCC AGCTGTGGGC TGCCCTGCCT CCGACAGCGA ATGGAACGGC 
     2761 GGCTGAAAGG ATCCCTGAAG ATGCTCAGAA AGTCCATCAA CCAGGACCGC TTCCTGCTGC 
     2821 GCCTGGCAGG CCTTGATTAT GAGCTGGCCC ACAAGCCGGG CCTGGTAGCC GGGGAGCGAG 
     2881 CAGAGCCGAT GGAGTCCTGT AGGCCCGGGC AGCACCGTGC TGGGACCAAG TGTGTCAGCT 
     2941 GCCCGCAGGG AACGTATTAC CACGGCCAGA CGGAGCAGTG TGTGCCATGC CCAGCGGGCA 
     3001 CCTTCCAGGA GAGAGAAGGG CAGCTCTCCT GCGACCTTTG CCCTGGGAGT GATGCCCACG 
     3061 GGCCTCTTGG AGCCACCAAC GTCACCACGT GTGCAGGTCA GTGCCCACCT GGCCAACACT 
     3121 CTGTAGATGG GTTCAAGCCC TGTCAGCCAT GCCCACGTGG CACCTACCAA CCTGAAGCAG 
     3181 GACGGACCCT ATGCTTCCCT TGTGGTGGGG GCCTCACCAC CAAGCATGAA GGGGCCATTT 
     3241 CCTTCCAAGA CTGTGACACC AAAGTCCAGT GCTCCCCAGG GCACTACTAC AACACCAGCA 
     3301 TCCACCGCTG TATTCGCTGT GCCATGGGCT CCTATCAGCC CGACTTCCGT CAGAACTTCT 
     3361 GCAGCCGCTG TCCAGGAAAC ACAAGCACAG ACTTTGATGG CTCTACCAGT GTGGCCCAAT 
     3421 GCAAGAATCG TCAGTGTGGT GGGGAGCTGG GTGAGTTCAC TGGCTATATT GAGTCCCCCA 
     3481 ACTACCCGGG CAACTACCCA GCTGGTGTGG AGTGCATCTG GAACATCAAC CCCCCACCCA 
     3541 AGCGCAAGAT CCTTATCGTG GTACCAGAGA TCTTCCTGCC ATCTGAGGAT GAGTGTGGGG 
     3601 ACGTCCTCGT CATGAGAAAG AACTCATCCC CATCCTCCAT TACCACTTAT GAGACCTGCC 
     3661 AGACCTACGA GCGTCCCATT GCCTTCACTG CCCGTTCCAG GAAGCTCTGG ATCAACTTCA 
     3721 AGACAAGCGA GGCCAACAGC GCCCGTGGCT TCCAGATTCC CTATGTTACC TATGATGAGG 
     3781 ACTATGAGCA GCTGGTAGAA GACATTGTGC GAGATGGCCG GCTCTATGCC TCTGAAAACC 
     3841 ACCAGGAGAT TTTAAAGGAC AAGAAGCTCA TCAAGGCCTT CTTTGAGGTG CTAGCCCACC 
     3901 CCCAGAACTA CTTCAAGTAC ACAGAGAAAC ACAAGGAGAT GCTGCCAAAA TCCTTCATCA 
     3961 AGCTGCTCCG CTCCAAAGTT TCCAGCTTCC TGAGGCCCTA CAAAACGCGT ACGCGGCCGC 
     4021 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     4081 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     4141 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     4201 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     4261 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4321 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4381 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4441 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4501 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4561 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4621 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4681 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4741 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4801 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4861 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4921 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4981 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5041 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5101 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5161 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5221 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5281 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5341 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5401 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5461 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5521 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5581 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5641 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5701 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5761 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5821 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5881 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5941 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     6001 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     6061 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     6121 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     6181 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     6241 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6301 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6361 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6421 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6481 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6541 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6601 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6661 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6721 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6781 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6841 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6901 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6961 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     7021 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     7081 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     7141 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     7201 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     7261 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7321 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7381 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7441 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7501 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7561 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7621 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7681 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7741 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7801 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7861 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7921 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7981 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     8041 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     8101 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     8161 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     8221 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     8281 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8341 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8401 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8461 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8521 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8581 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8641 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8701 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8761 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8821 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8881 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8941 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     9001 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     9061 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     9121 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     9181 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     9241 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9301 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9361 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9421 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9481 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//