LOCUS       RG239856                9521 bp    DNA     circular  SYN 
DEFINITION  BNC1 (tGFP-tagged) - Human basonuclin 1 (BNC1), transcript variant 2
ACCESSION   NM_001301206
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4002..4706 
                     /label="GFP"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    7430..8075 
                     /label="ColE1"  
     misc_feature    5792..6586 
                     /label="Neo"  
     misc_feature    5423..5725 
                     /label="SV40 ori"  
     misc_feature    8090..8950 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3981..3987 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3981 
                     /label="BNC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTGTAGA AACATGTTCT TCTCATTTAA GGCATCTCTT TGTGGCTGCG 
     1081 GGGCTGCCAC CGCTCCGAGT CTGACAGCTA TCAGCTGTAC TCTGAACTGT AGTTGCCAAA 
     1141 GTTTCAAACC CGGGAAAATA AACCACCGTC AGTGTGACCA ATGCAAGCAT GGATGGGTGG 
     1201 CCCACGCTCT AAGTAAGCTA AGGATCCCCC CCATGTATCC AACAAGCCAG GTGGAGATTG 
     1261 TCCAGTCCAA TGTAGTGTTT GATATTAGCA GCCTCATGCT CTATGGGACC CAGGCCATCC 
     1321 CCGTTCGCCT AAAAATCCTA CTGGACCGGC TCTTCAGTGT GTTGAAGCAA GATGAGGTTC 
     1381 TCCAGATCCT CCATGCCTTG GACTGGACAC TTCAGGATTA TATCCGTGGA TACGTACTGC 
     1441 AGGATGCATC AGGAAAGGTG TTGGATCACT GGAGCATCAT GACCAGTGAG GAAGAAGTGG 
     1501 CCACCTTGCA GCAGTTCCTT CGTTTTGGAG AGACCAAATC TATAGTTGAA CTCATGGCAA 
     1561 TTCAAGAGAA AGAAGAGCAA TCCATCATCA TACCACCTTC CACAGCAAAT GTAGATATCA 
     1621 GGGCTTTCAT CGAGAGCTGC AGTCACAGGA GTTCTAGCCT CCCCACTCCT GTGGACAAAG 
     1681 GAAACCCCAG CAGTATACAC CCCTTTGAGA ACCTCATAAG CAACATGACT TTCATGCTGC 
     1741 CTTTCCAGTT CTTCAACCCT CTGCCTCCTG CACTGATAGG GTCATTGCCC GAACAATATA 
     1801 TGTTGGAGCA GGGTCATGAC CAAAGTCAGG ACCCCAAACA GGAAGTCCAT GGGCCCTTCC 
     1861 CTGACAGCAG CTTCTTAACT TCCAGTTCCA CACCATTTCA GGTTGAAAAA GATCAGTGTT 
     1921 TAAACTGTCC GGATGCTATT ACTAAAAAAG AAGACAGCAC CCATTTAAGT GACTCCAGCT 
     1981 CATACAACAT TGTCACTAAG TTTGAAAGGA CACAGTTATC CCCTGAGGCC AAAGTGAAGC 
     2041 CTGAGAGGAA TAGCCTTGGT ACAAAGAAGG GCCGGGTGTT CTGCACTGCA TGTGAGAAGA 
     2101 CCTTCTATGA CAAAGGCACC CTCAAAATCC ACTACAATGC CGTCCACTTG AAGATCAAGC 
     2161 ATAAGTGCAC CATCGAAGGG TGTAACATGG TGTTCAGCTC CCTAAGGAGC CGGAATCGCC 
     2221 ATAGCGCCAA CCCCAACCCT CGGCTGCACA TGCCAATGAA CAGAAATAAC CGGGACAAAG 
     2281 ACCTCAGGAA CAGCCTGAAC CTGGCCAGCT CTGAGAACTA CAAGTGCCCA GGTTTCACAG 
     2341 TGACGTCCCC AGACTGTAGG CCTCCTCCCA GCTACCCTGG TTCAGGAGAG GATTCCAAAG 
     2401 GCCAACCAGC CTTCCCAAAC ATTGGGCAAA ATGGTGTGCT TTTTCCCAAC CTAAAGACAG 
     2461 TCCAGCCAGT CCTTCCTTTC TACCGCAGTC CAGCCACGCC TGCCGAGGTA GCAAACACGC 
     2521 CTGGGATACT CCCTTCCCTC CCGCTGTTGT CCTCTTCAAT CCCAGAACAG CTCATTTCAA 
     2581 ACGAAATGCC ATTTGATGCC CTTCCCAAGA AGAAATCCAG GAAGTCCAGT ATGCCTATCA 
     2641 AAATAGAGAA AGAAGCTGTG GAAATAGCTA ATGAGAAAAG ACACAACCTC AGCTCAGATG 
     2701 AAGACATGCC CCTACAGGTG GTCAGTGAAG ATGAGCAGGA GGCCTGCAGT CCTCAGTCAC 
     2761 ACAGAGTATC TGAGGAGCAG CATGTACAGT CAGGAGGCTT AGGGAAGCCT TTCCCTGAAG 
     2821 GGGAGAGGCC CTGCCATCGT GAATCAGTAA TTGAGTCCAG TGGAGCCATC AGCCAAACCC 
     2881 CTGAGCAGGC CACACACAAT TCAGAGAGGG AGACTGAGCA GACACCAGCA TTGATCATGG 
     2941 TGCCAAGGGA GGTCGAGGAT GGTGGCCATG AACACTACTT CACACCTGGG ATGGAACCCC 
     3001 AAGTTCCTTT TTCTGACTAC ATGGAACTGC AGCAGCGCCT GCTGGCTGGG GGACTCTTCA 
     3061 GTGCTTTGTC CAACAGGGGA ATGGCTTTTC CTTGTCTTGA AGATTCTAAA GAACTGGAGC 
     3121 ACGTGGGTCA GCATGCATTA GCAAGGCAGA TAGAAGAAAA TCGCTTCCAG TGTGACATCT 
     3181 GCAAGAAGAC CTTTAAAAAT GCTTGTAGTG TGAAAATTCA TCACAAGAAT ATGCATGTCA 
     3241 AAGAAATGCA CACATGCACA GTGGAGGGCT GTAATGCTAC CTTTCCCTCC CGCAGGAGCA 
     3301 GAGACAGACA CAGCTCAAAC CTAAACCTCC ACCAAAAAGC ATTGAGCCAG GAAGCATTGG 
     3361 AGAGTAGTGA AGATCATTTC CGTGCAGCTT ACCTTCTGAA AGATGTGGCT AAGGAAGCCT 
     3421 ATCAGGATGT GGCTTTTACA CAGCAAGCCT CCCAGACATC TGTCATCTTC AAAGGAACAA 
     3481 GTCGAATGGG CAGTCTGGTT TACCCAATAA CGCAAGTCCA CAGTGCCAGC CTGGAGAGCT 
     3541 ACAACTCTGG CCCCTTGAGC GAGGGCACCA TCCTGGATTT GAGCACTACC TCGAGCATGA 
     3601 AGTCAGAGAG TAGCAGCCAT TCTTCCTGGG ACTCTGACGG GGTGAGTGAG GAAGGCACTG 
     3661 TGCTTATGGA GGACAGTGAT GGGAACTGTG AAGGGTCGAG CCTTGTCCCT GGGGAAGATG 
     3721 AGTACCCCAT CTGTGTCCTG ATGGAGAAGG CTGACCAGAG CCTTGCTAGC CTGCCTTCTG 
     3781 GGTTGCCCAT AACCTGTCAT CTCTGCCAAA AGACATACAG TAACAAAGGG ACCTTTAGGG 
     3841 CCCACTACAA AACTGTGCAC CTCCGGCAGC TCCACAAATG CAAAGTACCA GGCTGCAACA 
     3901 CCATGTTTTC GTCTGTTCGC AGTCGAAACA GACACAGCCA GAATCCCAAC CTGCACAAAA 
     3961 GCCTGGCCTC ATCTCCAAGT CACCTCCAGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     4021 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     4081 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     4141 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     4201 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4261 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4321 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4381 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4441 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4501 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4561 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4621 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4681 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4741 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4801 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4861 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4921 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4981 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5041 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5101 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5161 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5221 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5281 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5341 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5401 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5461 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5521 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5581 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5641 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5701 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5761 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5821 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5881 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5941 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     6001 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     6061 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     6121 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     6181 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6241 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6301 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6361 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6421 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6481 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6541 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6601 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6661 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6721 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6781 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6841 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6901 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6961 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     7021 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     7081 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     7141 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     7201 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7261 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7321 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7381 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7441 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7501 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7561 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7621 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7681 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7741 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7801 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7861 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7921 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7981 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     8041 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     8101 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     8161 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     8221 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8281 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8341 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8401 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8461 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8521 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8581 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8641 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8701 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8761 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8821 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8881 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8941 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     9001 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     9061 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     9121 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     9181 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9241 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9301 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9361 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9421 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9481 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//