LOCUS       RG239844                9488 bp    DNA     circular  SYN 
DEFINITION  TEK (tGFP-tagged) - Human TEK tyrosine kinase, endothelial (TEK), transcript variant 3
ACCESSION   NM_001290078
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3969..4673 
                     /label="GFP"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    7397..8042 
                     /label="ColE1"  
     misc_feature    5759..6553 
                     /label="Neo"  
     misc_feature    5390..5692 
                     /label="SV40 ori"  
     misc_feature    8057..8917 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3948..3954 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3948 
                     /label="TEK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCAGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTCTG 
     1081 CTTCCTTCCT ACCAGCTACT TTAACTATGA CTGTGGACAA GGGAGATAAC GTGAACATAT 
     1141 CTTTCAAAAA GGTATTGATT AAAGAAGAAG ATGCAGTGAT TTACAAAAAT GGTTCCTTCA 
     1201 TCCATTCAGT GCCCCGGCAT GAAGTACCTG ATATTCTAGA AGTACACCTG CCTCATGCTC 
     1261 AGCCCCAGGA TGCTGGAGTG TACTCGGCCA GGTATATAGG AGGAAACCTC TTCACCTCGG 
     1321 CCTTCACCAG GCTGATAGTC CGGAGATGTG AAGCCCAGAA GTGGGGACCT GAATGCAACC 
     1381 ATCTCTGTAC TGCTTGTATG AACAATGGTG TCTGCCATGA AGATACTGGA GAATGCATTT 
     1441 GCCCTCCTGG GTTTATGGGA AGGACGTGTG AGAAGGCTTG TGAACTGCAC ACGTTTGGCA 
     1501 GAACTTGTAA AGAAAGGTGC AGTGGACAAG AGGGATGCAA GTCTTATGTG TTCTGTCTCC 
     1561 CTGACCCCTA TGGGTGTTCC TGTGCCACAG GCTGGAAGGG TCTGCAGTGC AATGAAGGCA 
     1621 TACCGAGGAT GACCCCAAAG ATAGTGGATT TGCCAGATCA TATAGAAGTA AACAGTGGTA 
     1681 AATTTAATCC CATTTGCAAA GCTTCTGGCT GGCCGCTACC TACTAATGAA GAAATGACCC 
     1741 TGGTGAAGCC GGATGGGACA GTGCTCCATC CAAAAGACTT TAACCATACG GATCATTTCT 
     1801 CAGTAGCCAT ATTCACCATC CACCGGATCC TCCCCCCTGA CTCAGGAGTT TGGGTCTGCA 
     1861 GTGTGAACAC AGTGGCTGGG ATGGTGGAAA AGCCCTTCAA CATTTCTGTT AAAGTTCTTC 
     1921 CAAAGCCCCT GAATGCCCCA AACGTGATTG ACACTGGACA TAACTTTGCT GTCATCAACA 
     1981 TCAGCTCTGA GCCTTACTTT GGGGATGGAC CAATCAAATC CAAGAAGCTT CTATACAAAC 
     2041 CCGTTAATCA CTATGAGGCT TGGCAACATA TTCAAGTGAC AAATGAGATT GTTACACTCA 
     2101 ACTATTTGGA ACCTCGGACA GAATATGAAC TCTGTGTGCA ACTGGTCCGT CGTGGAGAGG 
     2161 GTGGGGAAGG GCATCCTGGA CCTGTGAGAC GCTTCACAAC AGCTTCTATC GGACTCCCTC 
     2221 CTCCAAGAGG TCTAAATCTC CTGCCTAAAA GTCAGACCAC TCTAAATTTG ACCTGGCAAC 
     2281 CAATATTTCC AAGCTCGGAA GATGACTTTT ATGTTGAAGT GGAGAGAAGG TCTGTGCAAA 
     2341 AAAGTGATCA GCAGAATATT AAAGTTCCAG GCAACTTGAC TTCGGTGCTA CTTAACAACT 
     2401 TACATCCCAG GGAGCAGTAC GTGGTCCGAG CTAGAGTCAA CACCAAGGCC CAGGGGGAAT 
     2461 GGAGTGAAGA TCTCACTGCT TGGACCCTTA GTGACATTCT TCCTCCTCAA CCAGAAAACA 
     2521 TCAAGATTTC CAACATTACA CACTCCTCAG CTGTGATTTC TTGGACAATA TTGGATGGCT 
     2581 ATTCTATTTC TTCTATTACT ATCCGTTACA AGGTTCAAGG CAAGAATGAA GACCAGCACG 
     2641 TTGATGTGAA GATAAAGAAT GCCACCATCA CTCAGTATCA GCTCAAGGGC CTAGAGCCTG 
     2701 AAACAGCATA CCAGGTGGAC ATTTTTGCAG AGAACAACAT AGGGTCAAGC AACCCAGCCT 
     2761 TTTCTCATGA ACTGGTGACC CTCCCAGAAT CTCAAGCACC AGCGGACCTC GGAGGGGGGA 
     2821 AGATGCTGCT TATAGCCATC CTTGGCTCTG CTGGAATGAC CTGCCTGACT GTGCTGTTGG 
     2881 CCTTTCTGAT CATATTGCAA TTGAAGAGGG CAAATGTGCA AAGGAGAATG GCCCAAGCCT 
     2941 TCCAAAACAG GGAAGAACCA GCTGTGCAGT TCAACTCAGG GACTCTGGCC CTAAACAGGA 
     3001 AGGTCAAAAA CAACCCAGAT CCTACAATTT ATCCAGTGCT TGACTGGAAT GACATCAAAT 
     3061 TTCAAGATGT GATTGGGGAG GGCAATTTTG GCCAAGTTCT TAAGGCGCGC ATCAAGAAGG 
     3121 ATGGGTTACG GATGGATGCT GCCATCAAAA GAATGAAAGA ATATGCCTCC AAAGATGATC 
     3181 ACAGGGACTT TGCAGGAGAA CTGGAAGTTC TTTGTAAACT TGGACACCAT CCAAACATCA 
     3241 TCAATCTCTT AGGAGCATGT GAACATCGAG GCTACTTGTA CCTGGCCATT GAGTACGCGC 
     3301 CCCATGGAAA CCTTCTGGAC TTCCTTCGCA AGAGCCGTGT GCTGGAGACG GACCCAGCAT 
     3361 TTGCCATTGC CAATAGCACC GCGTCCACAC TGTCCTCCCA GCAGCTCCTT CACTTCGCTG 
     3421 CCGACGTGGC CCGGGGCATG GACTACTTGA GCCAAAAACA GTTTATCCAC AGGGATCTGG 
     3481 CTGCCAGAAA CATTTTAGTT GGTGAAAACT ATGTGGCAAA AATAGCAGAT TTTGGATTGT 
     3541 CCCGAGGTCA AGAGGTGTAT GTGAAAAAGA CAATGGGAAG GCTCCCAGTG CGCTGGATGG 
     3601 CCATCGAGTC ACTGAATTAC AGTGTGTACA CAACCAACAG TGATGTATGG TCCTATGGTG 
     3661 TGTTACTATG GGAGATTGTT AGCTTAGGAG GCACACCCTA CTGCGGGATG ACTTGTGCAG 
     3721 AACTCTACGA GAAGCTGCCC CAGGGCTACA GACTGGAGAA GCCCCTGAAC TGTGATGATG 
     3781 AGGTGTATGA TCTAATGAGA CAATGCTGGC GGGAGAAGCC TTATGAGAGG CCATCATTTG 
     3841 CCCAGATATT GGTGTCCTTA AACAGAATGT TAGAGGAGCG AAAGACCTAC GTGAATACCA 
     3901 CGCTTTATGA GAAGTTTACT TATGCAGGAA TTGACTGTTC TGCTGAAGAA GCGGCCACGC 
     3961 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     4021 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4081 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4141 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4201 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4261 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4321 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4381 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4441 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4501 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4561 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4621 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4681 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4741 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4801 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4861 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4921 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4981 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5041 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5101 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5161 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5221 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5281 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5341 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5401 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5461 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5521 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5581 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5641 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5701 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5761 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5821 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5881 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5941 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     6001 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6061 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6121 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6181 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6241 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6301 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6361 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6421 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6481 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6541 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6601 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6661 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6721 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6781 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6841 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6901 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6961 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     7021 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7081 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7141 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7201 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7261 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7321 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7381 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7441 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7501 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7561 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7621 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7681 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7741 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7801 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7861 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7921 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7981 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     8041 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8101 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8161 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8221 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8281 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8341 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8401 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8461 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8521 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8581 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8641 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8701 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8761 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8821 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8881 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8941 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9001 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9061 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9121 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9181 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9241 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9301 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9361 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9421 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9481 CGAATTTT
//