LOCUS       RG239790                9341 bp    DNA     circular  SYN 
DEFINITION  POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 5
ACCESSION   NM_001282776
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3820..4524 
                     /label="GFP"  
     misc_feature    4571..5156 
                     /label="PolyA signal"  
     misc_feature    7248..7893 
                     /label="ColE1"  
     misc_feature    5610..6404 
                     /label="Neo"  
     misc_feature    5241..5543 
                     /label="SV40 ori"  
     misc_feature    7908..8768 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3792..3799 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3792 
                     /label="POLR1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACTGTGTG AGGGAAGAAC ATTCCGCTGT CAATATGAAC CTCCACTACT 
     1081 TGGAAAATGG CACTGTTATG TTGAACTTTA TTTACCGAAA AGAACTGTTC TTTCTTCCTT 
     1141 TGGGATTTGC ACTTAAGGCA CTTGTCAGCT TTTCTGATTA TCAGATCTTT CAGGAGCTCA 
     1201 TCAAAGGAAA AGAGGATGAT TCTTTCCTTA GGAACTCTGT TTCTCAGATG TTAAGGATTG 
     1261 TAATGGAAGA GGGTTGTTCG ACACAAAAAC AGGTCCTTAA CTACCTAGGT GAATGCTTCA 
     1321 GAGTAAAACT CAATGTTCCT GACTGGTACC CAAATGAGCA AGCTGCGGAG TTCCTGTTTA 
     1381 ACCAGTGCAT CTGTATCCAC TTGAAATCCA ATACTGAAAA GTTTTATATG CTTTGTCTCA 
     1441 TGACGCGAAA GCTCTTTGCT TTAGCCAAAG GAGAGTGCAT GGAGGACAAT CCTGATAGTT 
     1501 TGGTGAACCA GGAAGTCCTC ACACCGGGTC AGCTCTTCCT TATGTTCCTG AAGGAAAAAC 
     1561 TGGAAGGTTG GTTAGTGTCT ATTAAAATAG CTTTTGATAA GAAGGCTCAG AAGACCAGTG 
     1621 TTTCCATGAA CACTGACAAT TTGATGAGGA TTTTTACAAT GGGCATAGAC CTTACAAAAC 
     1681 CATTTGAATA CCTTTTTGCT ACTGGGAATC TGCGTTCTAA AACAGGTCTT GGCCTCCTAC 
     1741 AAGATTCTGG ACTTTGTGTT GTGGCTGACA AGCTGAACTT CATACGCTAC CTCTCCCATT 
     1801 TCCGCTGCGT GCACAGAGGG GCTGATTTTG CCAAGATGAG GACCACCACA GTACGCAGGC 
     1861 TGCTGCCAGA GTCCTGGGGC TTCCTTTGTC CCGTGCATAC CCCAGACGGG GAGCCCTGTG 
     1921 GCCTGATGAA CCACCTAACT GCCGTATGTG AGGTTGTCAC ACAGTTTGTG TATACGGCAT 
     1981 CTATTCCAGC TTTACTGTGC AACTTGGGGG TCACTCCCAT TGATGGAGCT CCCCACCGAT 
     2041 CATACAGTGA GTGCTACCCT GTCCTGCTGG ACGGTGTCAT GGTTGGCTGG GTGGATAAGG 
     2101 ATCTTGCTCC AGGCATCGCA GATTCTCTTC GTCATTTTAA GGTGTTGAGA GAGAAAAGAA 
     2161 TTCCTCCCTG GATGGAAGTG GTCCTTATAC CCATGACAGG AAAACCAAGT CTGTACCCAG 
     2221 GATTGTTCCT TTTTACCACT CCTTGTAGAC TGGTACGGCC TGTGCAGAAC TTAGCATTGG 
     2281 GCAAAGAAGA GCTAATTGGA ACTATGGAAC AGATCTTCAT GAATGTCGCT ATCTTTGAGG 
     2341 ATGAAGTTTT TGCTGGAGTT ACCACACACC AGGAACTCTT TCCACACAGC CTGCTGAGTG 
     2401 TGATTGCCAA CTTCATCCCT TTCTCTGATC ACAACCAGAG TCCACGGAAC ATGTACCAAT 
     2461 GCCAGATGGG TAAGCAAACT ATGGGCTTTC CACTTCTCAC TTATCAAGAC CGATCGGATA 
     2521 ACAAACTGTA TCGTCTTCAG ACTCCTCAGA GTCCCTTGGT GAGACCCTCC ATGTATGATT 
     2581 ATTATGACAT GGATAACTAT CCAATTGGGA CCAATGCCAT CGTTGCTGTG ATTTCTTACA 
     2641 CTGGCTATGA TATGGAAGAT GCCATGATTG TGAATAAGGC CTCTTGGGAA CGAGGCTTTG 
     2701 CCCATGGAAG TGTCTACAAG TCTGAGTTCA TAGACCTCTC TGAAAAAATT AAACAAGGAG 
     2761 ATAGTAGCCT GGTGTTTGGC ATCAAACCTG GTGACCCACG CGTTCTGCAG AAGTTAGATG 
     2821 ACGATGGATT GCCGTTTATA GGAGCAAAAC TGCAGTACGG AGATCCGTAT TACAGCTACC 
     2881 TCAACCTCAA CACCGGGGAA AGTTTTGTGA TGTACTATAA GAGTAAAGAA AATTGTGTTG 
     2941 TGGATAACAT CAAAGTGTGC AGTAATGACA CTGGGAGTGG AAAATTCAAG TGTGTTTGCA 
     3001 TCACTATGAG AGTGCCTCGG AACCCAACTA TCGGAGATAA ATTTGCCAGT CGCCATGGGC 
     3061 AGAAGGGCAT TTTAAGCAGA TTGTGGCCGG CTGAGGACAT GCCTTTTACT GAGAGTGGGA 
     3121 TGGTCCCAGA CATTCTGTTC AATCCCCATG GTTTTCCATC CCGCATGACC ATTGGGATGT 
     3181 TAATTGAGAG TATGGCCGGG AAGTCTGCAG CTTTGCATGG TCTCTGCCAT GATGCTACAC 
     3241 CCTTCATCTT CTCAGAGGAG AACTCGGCCT TAGAATACTT TGGTGAGATG TTAAAGGCTG 
     3301 CTGGCTACAA TTTCTATGGC ACCGAGAGGT TATATAGTGG CATCAGTGGG CTAGAACTGG 
     3361 AAGCAGACAT CTTCATAGGA GTGGTTTATT ATCAGCGCTT ACGCCATATG GTCTCAGACA 
     3421 AATTTCAAGT AAGGACAACT GGAGCCCGAG ACAGAGTCAC CAACCAGCCT ATTGGGGGAA 
     3481 GAAATGTCCA GGGTGGAATC CGTTTTGGGG AGATGGAACG GGATGCGCTT TTAGCTCATG 
     3541 GTACATCTTT TCTCCTTCAT GACCGCCTCT TCAACTGCTC AGATCGGTCG GTAGCCCATG 
     3601 TGTGTGTGAA GTGTGGCAGT TTACTCTCTC CACTGTTGGA GAAGCCACCC CCTTCTTGGT 
     3661 CTGCCATGCG CAACAGAAAA TACAACTGTA CTCTGTGTAG TCGCAGTGAC ACTATCGATA 
     3721 CTGTTTCTGT GCCTTATGTT TTTCGGTATT TTGTAGCTGA ACTGGCAGCT ATGAACATCA 
     3781 AAGTGAAACT GGATGTTGTT AGCGGACCGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3841 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3901 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3961 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     4021 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4081 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4141 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4201 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4261 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4321 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4381 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4441 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4501 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5221 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5281 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5341 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5401 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5461 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5521 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5581 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5641 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5701 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5761 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5821 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5881 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5941 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     6001 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6061 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6121 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6181 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6241 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6301 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6361 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6421 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6481 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6541 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6601 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6661 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6721 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6781 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6841 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6901 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6961 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     7021 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7081 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7141 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7201 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7261 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7321 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7381 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7441 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7501 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7561 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7621 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7681 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7741 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7801 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7861 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7921 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7981 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     8041 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8101 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8161 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8221 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8281 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8341 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8401 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8461 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8521 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8581 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8641 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8701 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8761 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8821 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8881 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8941 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     9001 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9061 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9121 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9181 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9241 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9301 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//