LOCUS       RG239785                9317 bp    DNA     circular  SYN 
DEFINITION  DGKH (tGFP-tagged) - Human diacylglycerol kinase, eta (DGKH), transcript variant 6
ACCESSION   NM_001297429
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3798..4502 
                     /label="GFP"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    7226..7871 
                     /label="ColE1"  
     misc_feature    5588..6382 
                     /label="Neo"  
     misc_feature    5219..5521 
                     /label="SV40 ori"  
     misc_feature    7886..8746 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3783 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="DGKH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAACATT TCTCAGGGAT GCACAACTGG TACGCCTGCT CCCACGCCCG 
     1081 ACCCACCTTC TGTAACGTGT GCAGAGAGAG TCTTTCTGGA GTCACCTCCC ATGGCCTGTC 
     1141 CTGCGAAGGT ACACACTGCC TGCAAAGATT TATACCATCC AATATGTCCA CTTGGTCAAT 
     1201 GTAAAGTATC TATCATACCT CCAATTGCAC TAAACAGCAC CGATTCCGAT GGTTTCTGTA 
     1261 GAGCAACATT TTCGTTCTGT GTTAGTCCTC TATTGGTTTT TGTCAATTCT AAGAGTGGAG 
     1321 ATAATCAGGG AGTAAAGTTC CTCCGTCGCT TTAAACAGTT GCTAAATCCG GCTCAGGTGT 
     1381 TTGATTTAAT GAATGGAGGT CCTCATTTAG GTTTAAGATT ATTTCAGAAG TTTGACAATT 
     1441 TCCGGATTCT TGTTTGTGGA GGCGATGGAA GTGTAGGTTG GGTTTTGTCA GAAATCGATA 
     1501 AGCTCAACTT GAATAAACAG TGTCAGCTGG GAGTGTTGCC TTTGGGTACA GGAAATGACC 
     1561 TTGCCCGAGT TCTTGGCTGG GGAGGTTCAT ATGACGATGA CACCCAACTT CCTCAGATCC 
     1621 TAGAGAAACT GGAACGAGCC AGTACCAAAA TGTTGGACAG GTGGAGTATA ATGACATATG 
     1681 AACTCAAATT GCCACCAAAA GCTTCCCTAC TTCCAGGACC TCCAGAAGCA TCTGAAGAAT 
     1741 TTTATATGAC GATTTATGAA GACTCAGTTG CAACGCATCT TACAAAAATC CTCAATTCTG 
     1801 ATGAACATGC AGTGGTCATA TCTTCTGCCA AGACGCTATG TGAAACTGTA AAGGACTTCG 
     1861 TTGCCAAAGT AGAAAAGACG TATGACAAAA CCTTGGAAAA TGCCGTTGTA GCTGATGCCG 
     1921 TGGCCAGTAA ATGTTCAGTC CTAAACGAGA AGCTCGAACA ACTGCTGCAG GCTTTGCACA 
     1981 CAGATTCCCA GGCTGCGCCT GTTCTCCCTG GCCTCAGCCC TCTCATTGTG GAAGAAGATG 
     2041 CTGTGGAATC GTCCAGTGAA GAGTCCCTGG GTGAAAGCAA AGAGCAGCTT GGGGATGACG 
     2101 TTACAAAACC TTCCTCCCAG AAAGCCGTCA AACCAAGGGA AATCATGTTG CGGGCAAATA 
     2161 GTTTAAAGAA AGCAGTGAGG CAAGTCATTG AGGAAGCCGG AAAAGTTATG GATGACCCGA 
     2221 CAGTTCACCC CTGTGAACCA GCTAATCAGT CCTCTGATTA TGACAGCACA GAAACAGATG 
     2281 AATCTAAGGA GGAAGCTAAA GATGATGGTG CCAAAGAATC AATAACTGTT AAAACTGCAC 
     2341 CTCGGTCTCC AGATGCCCGG GCAAGTTATG GCCATTCCCA AACTGATTCT GTCCCTGGTC 
     2401 CAGCTGTGGC AGCCAGCAAA GAAAACCTCC CTGTGCTCAA TACCAGAATA ATCTGCCCAG 
     2461 GTTTAAGAGC AGGACTGGCT GCCTCAATTG CTGGGAGTTC GATTATCAAC AAAATGTTAC 
     2521 TGGCAAACAT TGATCCTTTT GGTGCCACGC CGTTTATTGA CCCGGATCTA GATTCCGTAG 
     2581 ATGGATATTC AGAAAAATGT GTCATGAACA ATTACTTTGG GATTGGATTA GATGCAAAAA 
     2641 TTTCATTAGA ATTTAATAAT AAAAGAGAGG AGCACCCTGA AAAATGCAGG AGCCGAACTA 
     2701 AAAACTTGAT GTGGTATGGA GTCCTTGGAA CCCGGGAGTT ATTACAGAGA TCGTACAAGA 
     2761 ATTTAGAACA AAGGGTTCAA CTTGAGTGTG ATGGGCAGTA TATTCCTCTT CCCAGCTTGC 
     2821 AAGGCATAGC CGTGTTGAAC ATTCCCAGCT ATGCTGGAGG CACTAACTTT TGGGGTGGAA 
     2881 CTAAAGAGGA TGATATATTT GCTGCACCAT CCTTTGATGA CAAGATCCTG GAAGTTGTAG 
     2941 CAATATTTGA TAGCATGCAA ATGGCAGTTT CAAGGGTCAT TAAACTGCAG CATCATCGAA 
     3001 TAGCCCAGTG CCGTACAGTG AAAATCACTA TATTTGGTGA CGAAGGAGTC CCAGTGCAAG 
     3061 TGGATGGTGA AGCGTGGGTT CAGCCTCCAG GGATTATCAA AATTGTGCAC AAAAACAGAG 
     3121 CACAAATGCT AACAAGGGAC AGAGCCTTTG AGAGCACTCT GAAATCTTGG GAAGATAAGC 
     3181 AGAAGTGTGA TTCTGGTAAA CCAGTTCTCC GAACCCATTT GTACATCCAT CACGCCATTG 
     3241 ACTTGGCAAC AGAAGAGGTG TCGCAGATGC AGCTATGCTC CCAGGCTGCA GAGGAGCTCA 
     3301 TTACTAGGAT ATGTGACGCA GCCACAATTC ACTGTCTTTT GGAGCAAGAA CTGGCCCATG 
     3361 CTGTGAATGC CTGCTCCCAT GCCCTGAATA AAGCCAACCC AAGGTGCCCG GAGAGTCTTA 
     3421 CAAGAGACAC TGCCACTGAA ATAGCCATCA ATGTGAAGGC GCTGTATAAT GAAACAGAAT 
     3481 CTTTGCTAGT TGGCAGGGTT CCTTTGCAGC TGGAATCGCC ACATGAAGAG CGAGTATCCA 
     3541 ATGCCTTACA CTCTGTGGAG GTGGAATTAC AGAAACTGAC AGAGATTCCT TGGCTTTATT 
     3601 ATATCTTACA CCCAAATGAG GATGAGGAAC CTCCTATGGA TTGCACCAAA AGGAACAACA 
     3661 GAAGCACCGT ATTTCGAATA GTGCCAAAGT TTAAAAAGGA AAAGGTTCAG AAGCAGAAGA 
     3721 CAAGTTCACA GCCTGGATCT GGGGATACCG AAAGTGGGTC ATGTGAAGCG AATTCTCCAG 
     3781 GGAATACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3841 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3901 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3961 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4021 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4081 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4141 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4201 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4261 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4321 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4381 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4441 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4501 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5221 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5281 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5341 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5401 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5461 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5521 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5581 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5641 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5701 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5761 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5821 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5881 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5941 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6001 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6061 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6121 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6181 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6241 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6301 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6361 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6421 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6481 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6541 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6601 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6661 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6721 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6781 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6841 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6901 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6961 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7021 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7081 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7141 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7201 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7261 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7321 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7381 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7441 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7501 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7561 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7621 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7681 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7741 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7801 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7861 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7921 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7981 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8041 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8101 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8161 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8221 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8281 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8341 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8401 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8461 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8521 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8581 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8641 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8701 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8761 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8821 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8881 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8941 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9001 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9061 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9121 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9181 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9241 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9301 AATTTAACGC GAATTTT
//