LOCUS       RG239733                9212 bp    DNA     circular  SYN 
DEFINITION  HELLS (tGFP-tagged) - Human helicase, lymphoid-specific (HELLS), transcript variant 2
ACCESSION   NM_001289067
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3693..4397 
                     /label="GFP"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    7121..7766 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Neo"  
     misc_feature    5114..5416 
                     /label="SV40 ori"  
     misc_feature    7781..8641 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="HELLS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCGGAA CGGCCCGCGG GCAGCGGCGG CTCGGAGGCT CCAGCAATGG 
     1081 TTGAACAACT GGACACTGCT GTGATTACCC CGGCCATGCT AGAAGAGGAA GAACAGCTTG 
     1141 AAGCTGCTGG ACTAGAGAGA GAGCGGAAGA TGCTGGAAAA GGCTCGCATG TCTTGGGATA 
     1201 GAGAGTCGAC AGAAATTCGG TACCGTAGAC TTCAACATTT GCTTGAAAAA AGCAATATAT 
     1261 ACTCCAAATT TTTATTGACG AAAATGGAAC AGCAACAATT AGAGGAACAG AAGAAGAAAG 
     1321 AAAAATTGGA GAGAAAAAAG GAGTCTTTAA AAGTTAAAAA GGGTAAAAAT TCAATTGATG 
     1381 CAAGTGAAGA GAAGCCAGTT ATGAGGAAAA AAAGAGGAAG AGAAGATGAA TCATACAATA 
     1441 TTTCAGAGGT CATGTCAAAA GAGGAAATTT TGTCTGTGGC TAAAAAAAAT AAAAAGGAGA 
     1501 ATGAGGATGA AAACTCCTCC TCTACTAATC TCTGTGTGGA AGATCTTCAG AAAAATAAAG 
     1561 ATTCGAATAG TATAATTAAA GATAGATTGT CTGAAACGGT TAGGCAGAAT ACTAAATTCT 
     1621 TTTTTGACCC AGTCCGGAAG TGTAATGGTC AGCCAGTACC TTTTCAACAA CCAAAGCACT 
     1681 TCACTGGAGG AGTGATGCGA TGGTACCAAG TAGAAGGCAT GGAATGGCTT AGGATGCTTT 
     1741 GGGAAAATGG AATTAATGGC ATTTTAGCAG ATGAAATGGG ATTGGGTAAG ACAGTTCAGT 
     1801 GCATTGCTAC TATTGCATTG ATGATTCAGA GAGGAGTACC AGGACCTTTT CTTGTCTGTG 
     1861 GCCCTTTGTC TACACTTCCT AACTGGATGG CTGAATTCAA AAGATTTACA CCAGATATCC 
     1921 CTACAATGTT ATATCATGGA ACCCAGGAGG AACGTCAAAA ATTGGTAAGA AATATTTACA 
     1981 AACGGAAAGG GACTTTGCAG ATTCATCCTG TGGTAATCAC GTCATTTGAA ATAGCCATGA 
     2041 GAGACCGAAA TGCGTTACAG CATTGCTATT GGAAATACTT AATAGTAGAT GAAGGACACA 
     2101 GGATTAAGAA TATGAAGTGC CGTCTAATCA GGGAGTTAAA ACGATTCAAT GCTGATAACA 
     2161 AACTTCTTTT GACTGGTACT CCCTTGCAAA ACAATTTATC AGAACTTTGG TCATTGCTAA 
     2221 ACTTTTTGTT GCCAGATGTA TTTGATGACT TGAAAAGCTT TGAGTCTTGG TTTGACATCA 
     2281 CTAGTCTTTC TGAAACTGCT GAAGATATTA TTGCTAAAGA AAGAGAACAG AATGTATTGC 
     2341 ATATGCTGCA CCAGGAAAGA ATTCCTAGAA TGTATGTGTC AGAGTTTACA GATGAGGGCT 
     2401 TCTTTGAAGG CAAATTGCCC TCCAGAAAAG ATTTATATGA TATACGTCCA TATGAAAATA 
     2461 CTTATTTACT CAAGCTTCAT ACCAATTTTG AGATTTTAAC ACCTTTCTTA TTGAGAAGAC 
     2521 TGAAGTCTGA TGTTGCTCTT GAAGTTCCTC CTAAACGAGA AGTAGTCGTT TATGCTCCAC 
     2581 TTTCAAAGAA GCAGGAGATC TTTTATACAG CCATTGTGAA CCGTACAATT GCAAACATGT 
     2641 TTGGATCCAG TGAGAAAGAA ACAATTGAGT TAAGTCCTAC TGGTCGACCA AAACGACGAA 
     2701 CTAGAAAATC AATAAATTAC AGCAAAATAG ATGATTTCCC TAATGAATTG GAAAAACTGA 
     2761 TCAGTCAAAT ACAGCCAGAG GTGGACCGAG AAAGAGCTGT TGTGGAAGTG AATATCCCTG 
     2821 TAGAATCTGA AGTTAATCTG AAGCTGCAGA ATATAATGAT GCTACTTCGT AAATGTTGTA 
     2881 ATCATCCATA TTTGATTGAA TATCCTATAG ACCCTGTTAC ACAAGAATTT AAGATCGATG 
     2941 AAGAATTGGT AACAAATTCT GGGAAGTTCT TGATTTTGGA TCGAATGCTG CCAGAACTAA 
     3001 AAAAAAGAGG TCACAAGGTG CTGCTTTTTT CACAAATGAC AAGCATGTTG GACATTTTGA 
     3061 TGGATTACTG CCATCTCAGA GATTTCAACT TCAGCAGGCT TGATGGGTCC ATGTCTTACT 
     3121 CAGAGAGAGA AAAAAACATG CACAGCTTCA ACACGGATCC AGAGGTGTTT ATCTTCTTAG 
     3181 TGAGTACACG AGCTGGTGGC CTGGGCATTA ATCTGACTGC AGCAGATACA GTTATCATTT 
     3241 ATGATAGTGA TTGGAACCCC CAGTCGGATC TTCAGGCCCA GGATAGATGT CATAGAATTG 
     3301 GTCAGACAAA GCCAGTTGTT GTTTATCGCC TTGTTACAGC AAATACTATC GATCAGAAAA 
     3361 TTGTGGAAAG AGCAGCTGCT AAAAGGAAAC TGGAAAAGTT GATCATCCAT AAAAATCATT 
     3421 TCAAAGGTGG TCAGTCTGGA TTAAATCTGT CTAAGAATTT CTTAGATCCT AAGGAATTAA 
     3481 TGGAATTATT AAAATCTAGA GATTATGAAA GGGAAATAAA AGGATCAAGA GAGAAGGTCA 
     3541 TTAGTGATAA AGATCTAGAG TTGTTGTTAG ATCGAAGTGA TCTTATTGAT CAAATGAATG 
     3601 CTTCAGGACC AATTAAAGAG AAGATGGGGA TATTCAAGAT ATTAGAAAAT TCTGAAGATT 
     3661 CCAGTCCTGA ATGTTTGTTT ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3721 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3781 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3841 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3901 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3961 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4021 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4081 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4141 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4201 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4261 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4321 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4381 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5101 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5281 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5341 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5401 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5461 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5521 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5581 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5641 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5701 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5761 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5821 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5881 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5941 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6001 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6061 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6121 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6181 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6241 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6301 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6361 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6421 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6481 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6541 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6601 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6661 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6721 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6781 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6841 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6901 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6961 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7021 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7081 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7141 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7201 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7261 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7321 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7381 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7441 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7501 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7561 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7621 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7681 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7741 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7801 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7861 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7921 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7981 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8041 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8101 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8161 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8221 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8281 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8341 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8401 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8461 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8521 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8581 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8641 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8701 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8761 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8821 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8881 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8941 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9001 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9061 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9121 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9181 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//