LOCUS       RG239732                9212 bp    DNA     circular  SYN 
DEFINITION  NCAM1 (tGFP-tagged) - Human neural cell adhesion molecule 1 (NCAM1), transcript variant 5
ACCESSION   NM_001242607
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3693..4397 
                     /label="GFP"  
     misc_feature    4444..5029 
                     /label="PolyA signal"  
     misc_feature    7121..7766 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Neo"  
     misc_feature    5114..5416 
                     /label="SV40 ori"  
     misc_feature    7781..8641 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="NCAM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCAAACT AAGGATCTCA TCTGGACTTT GTTTTTCCTG GGAACTGCAG 
     1081 TTTCTCTGCA GGTGGATATT GTTCCCAGCC AGGGGGAGAT CAGCGTTGGA GAGTCCAAAT 
     1141 TCTTCTTATG CCAAGTGGCA GGAGATGCCA AAGATAAAGA CATCTCCTGG TTCTCCCCCA 
     1201 ATGGAGAAAA GCTCACCCCA AACCAGCAGC GGATCTCAGT GGTGTGGAAT GATGATTCCT 
     1261 CCTCCACCCT CACCATCTAT AACGCCAACA TCGACGACGC CGGCATTTAC AAGTGTGTGG 
     1321 TTACAGGCGA GGATGGCAGT GAGTCAGAGG CCACCGTCAA CGTGAAGATC TTTCAGAAGC 
     1381 TCATGTTCAA GAATGCGCCA ACCCCACAGG AGTTCCGGGA GGGGGAAGAT GCCGTGATTG 
     1441 TGTGTGATGT GGTCAGCTCC CTCCCACCAA CCATCATCTG GAAACACAAA GGCCGAGATG 
     1501 TCATCCTGAA AAAAGATGTC CGATTCATAG TCCTGTCCAA CAACTACCTG CAGATCCGGG 
     1561 GCATCAAGAA AACAGATGAG GGCACTTATC GCTGTGAGGG CAGAATCCTG GCACGGGGGG 
     1621 AGATCAACTT CAAGGACATT CAGGTCATTG TGAATGTGCC ACCTACCATC CAGGCCAGGC 
     1681 AGAATATTGT GAATGCCACC GCCAACCTCG GCCAGTCCGT CACCCTGGTG TGCGATGCCG 
     1741 AAGGCTTCCC AGAGCCCACC ATGAGCTGGA CAAAGGATGG GGAACAGATA GAGCAAGAGG 
     1801 AAGACGATGA GAAGTACATC TTCAGCGACG ATAGTTCCCA GCTGACCATC AAAAAGGTGG 
     1861 ATAAGAACGA CGAGGCTGAG TACATCTGCA TTGCTGAGAA CAAGGCTGGC GAGCAGGATG 
     1921 CGACCATCCA CCTCAAAGTC TTTGCAAAAC CCAAAATCAC ATATGTAGAG AACCAGACTG 
     1981 CCATGGAATT AGAGGAGCAG GTCACTCTTA CCTGTGAAGC CTCCGGAGAC CCCATTCCCT 
     2041 CCATCACCTG GAGGACTTCT ACCCGGAACA TCAGCAGCGA AGAAAAGGCT TCGTGGACTC 
     2101 GACCAGAGAA GCAAGAGGTA CATGCACCAT GGAACTGGCA AGTGGGCAGA CAGAAAGGAC 
     2161 AGGCTGGCAG TGCAGGTTTC CCAGGATCTC ATGAGACTCT GGATGGGCAC ATGGTGGTGC 
     2221 GTAGCCATGC CCGTGTGTCG TCGCTGACCC TGAAGAGCAT CCAGTACACT GATGCCGGAG 
     2281 AGTACATCTG CACCGCCAGC AACACCATCG GCCAGGACTC CCAGTCCATG TACCTTGAAG 
     2341 TGCAATATGC CCCAAAGCTA CAGGGCCCTG TGGCTGTGTA CACTTGGGAG GGGAACCAGG 
     2401 TGAACATCAC CTGCGAGGTA TTTGCCTATC CCAGTGCCAC GATCTCATGG TTTCGGGATG 
     2461 GCCAGCTGCT GCCAAGCTCC AATTACAGCA ATATCAAGAT CTACAACACC CCCTCTGCCA 
     2521 GCTATCTGGA GGTGACCCCA GACTCTGAGA ATGATTTTGG GAACTACAAC TGTACTGCAG 
     2581 TGAACCGCAT TGGGCAGGAG TCCTTGGAAT TCATCCTTGT TCAAGCAGAC ACCCCCTCTT 
     2641 CACCATCCAT CGACCAGGTG GAGCCATACT CCAGCACAGC CCAGGTGCAG TTTGATGAAC 
     2701 CAGAGGCCAC AGGTGGGGTG CCCATCCTCA AATACAAAGC TGAGTGGAGA GCAGTTGGTG 
     2761 AAGAAGTATG GCATTCCAAG TGGTATGATG CCAAGGAAGC CAGCATGGAG GGCATCGTCA 
     2821 CCATCGTGGG CCTGAAGCCC GAAACAACGT ACGCCGTAAG GCTGGCGGCG CTCAATGGCA 
     2881 AAGGGCTGGG TGAGATCAGC GCGGCCTCCG AGTTCAAGAC GCAGCCAGTC CAAGGGGAAC 
     2941 CCAGTGCACC TAAGCTCGAA GGGCAGATGG GAGAGGATGG AAACTCTATT AAAGTGAACC 
     3001 TGATCAAGCA GGATGACGGC GGCTCCCCCA TCAGACACTA TCTGGTCAGG TACCGAGCGC 
     3061 TCTCCTCCGA GTGGAAACCA GAGATCAGGC TCCCGTCTGG CAGTGACCAC GTCATGCTGA 
     3121 AGTCCCTGGA CTGGAATGCT GAGTATGAGG TCTACGTGGT GGCTGAGAAC CAGCAAGGAA 
     3181 AATCCAAGGC GGCTCATTTT GTGTTCAGGA CCTCGGCCCA GCCCACAGCC ATCCCAGCCA 
     3241 ACGGCAGCCC CACCTCAGGC CTGAGCACCG GGGCCATCGT GGGCATCCTC ATCGTCATCT 
     3301 TCGTCCTGCT CCTGGTGGTT GTGGACATCA CCTGCTACTT CCTGAACAAG TGTGGCCTGT 
     3361 TCATGTGCAT TGCGGTCAAC CTGTGTGGAA AAGCCGGGCC CGGGGCCAAG GGCAAGGACA 
     3421 TGGAGGAGGG CAAGGCCGCC TTCTCGAAAG ATGAGTCCAA GGAGCCCATC GTGGAGGTTC 
     3481 GAACGGAGGA GGAGAGGACC CCAAACCATG ATGGAGGGAA ACACACAGAG CCCAACGAGA 
     3541 CCACGCCACT GACGGAGCCC GAGAAGGGCC CCGTAGAAGC AAAGCCAGAG TGCCAGGAGA 
     3601 CAGAAACGAA GCCAGCGCCA GCCGAAGTCA AGACGGTCCC CAATGACGCC ACACAGACAA 
     3661 AGGAGAACGA GAGCAAAGCA ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3721 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3781 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3841 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3901 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3961 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4021 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4081 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4141 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4201 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4261 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4321 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4381 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5101 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5281 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5341 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5401 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5461 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5521 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5581 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5641 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5701 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5761 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5821 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5881 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5941 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6001 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6061 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6121 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6181 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6241 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6301 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6361 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6421 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6481 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6541 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6601 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6661 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6721 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6781 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6841 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6901 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6961 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7021 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7081 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7141 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7201 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7261 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7321 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7381 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7441 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7501 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7561 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7621 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7681 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7741 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7801 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7861 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7921 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7981 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8041 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8101 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8161 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8221 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8281 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8341 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8401 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8461 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8521 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8581 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8641 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8701 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8761 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8821 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8881 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8941 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9001 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9061 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9121 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9181 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//