LOCUS       RG239701                9146 bp    DNA     circular  SYN 
DEFINITION  BRD8 (tGFP-tagged) - Human bromodomain containing 8 (BRD8), transcript variant 5
ACCESSION   NM_001300961
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3627..4331 
                     /label="GFP"  
     misc_feature    4378..4963 
                     /label="PolyA signal"  
     misc_feature    7055..7700 
                     /label="ColE1"  
     misc_feature    5417..6211 
                     /label="Neo"  
     misc_feature    5048..5350 
                     /label="SV40 ori"  
     misc_feature    7715..8575 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3606..3612 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3606 
                     /label="BRD8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCATCCG AGAGAAGCTA TGTTTAGCAT CTTCTGTCAT GAGAAGTGGC 
     1081 GATCAAAATT GGTAAGGGTA TCAGTTAGCA GAGCAATCAA GCCCTTTGCA GAACCTGGCC 
     1141 GCCCTCCAGA CTGGTTCTCT CAAAAACATT GTGCTTCCCA GTACTCGGAG CTTTTAGAGA 
     1201 CCACTGAGAC ACCAAAACGG AAACGAGGTG AAAAGGGAGA AGTGGTGGAA ACTGTTGAAG 
     1261 ATGTTATTGT TCGGAAATTG ACTGCTGAGC GAGTTGAAGA ACTAAAGAAA GTGATAAAGG 
     1321 AAACCCAGGA GAGATATAGA CGGCTAAAGA GAGATGCAGA ACTAATTCAA GCTGGACACA 
     1381 TGGACAGCAG ACTGGATGAG CTTTGCAATG ACATTGCAAC GAAAAAGAAA TTGGAAGAAG 
     1441 AGGAGGCTGA AGTAAAGAGG AAGGCTACAG ATGCTGCATA CCAGGCTCGT CAAGCAGTAA 
     1501 AAACACCCCC CCGGAGGTTA CCCACTGTGA TGGTTCGCTC TCCTATAGAT TCTGCCTCCC 
     1561 CAGGAGGTGA TTATCCACTT GGGGACTTGA CTCCAACCAC TATGGAAGAG GCTACCTCTG 
     1621 GGGTCAATGA GAGTGAAATG GCTGTGGCTT CTGGCCACCT GAACAGTACA GGTGTCCTCC 
     1681 TGGAGGTAGG CGGGGTCCTT CCCATGATAC ATGGTGGGGA GATACAGCAA ACACCCAATA 
     1741 CTGTTGCAGC CTCCCCTGCT GCATCAGGTG CTCCCACTCT TTCCCGGCTT TTAGAAGCTG 
     1801 GTCCTACACA GTTCACCACA CCTCTTGCTT CCTTCACTAC TGTTGCCAGT GAGCCTCCAG 
     1861 TTAAACTTGT GCCACCCCCT GTAGAGTCTG TGTCCCAAGC TACCATTGTC ATGATGCCTG 
     1921 CGCTGCCAGC ACCATCCTCT GCTCCGGCTG TCTCCACTAC TGAAAGTGTA GCTCCAGTGA 
     1981 GTCAACCCGA CAACTGTGTT CCCATGGAGG CTGTGGGGGA TCCACATACT GTGACTGTTT 
     2041 CCATGGACAG CAGTGAAATA TCCATGATCA TCAATTCTAT CAAAGAAGAG TGTTTTCGAT 
     2101 CAGGGGTAGC AGAGGCTCCT GTTGGATCAA AGGCTCCCAG CATAGATGGG AAGGAAGAAT 
     2161 TAGATCTGGC TGAGAAGATG GATATTGCTG TGTCTTACAC AGGTGAAGAG CTGGATTTTG 
     2221 AGACTGTTGG AGACATCATT GCCATCATTG AGGACAAGGT AGATGATCAT CCTGAAGTGC 
     2281 TGGATGTGGC AGCAGTGGAA GCAGCACTGT CATTTTGTGA AGAAAATGAT GATCCTCAGT 
     2341 CCCTGCCTGG CCCCTGGGAG CATCCTATCC AGCAGGAGCG GGACAAGCCA GTACCTCTCC 
     2401 CTGCACCAGA AATGACGGTC AAGCAAGAGA GACTGGACTT TGAGGAAACG GAAAACAAGG 
     2461 GAATACATGA ACTGGTGGAC ATCAGGGAGC CCAGTGCAGA GATCAAGGTG GAACCTGCAG 
     2521 AACCAGAGCC AGTCATTTCA GGAGCCGAAA TAGTAGCTGG AGTTGTTCCA GCCACAAGTA 
     2581 TGGAGCCACC AGAACTCAGG AGTCAGGACT TAGATGAGGA ACTGGGAAGT ACTGCAGCTG 
     2641 GAGAGATTGT TGAAGCAGAT GTTGCCATTG GGAAAGGCGA TGAGACTCCA CTTACAAATG 
     2701 TGAAGACAGA GGCATCCCCT GAAAGCATGT TGTCTCCATC ACATGGCTCA AATCCCATTG 
     2761 AAGATCCTTT AGAGGCAGAG ACTCAGCACA AGTTTGAAAT GTCAGACTCA TTGAAAGAAG 
     2821 AATCAGGGAC TATTTTTGGA AGCCAGATAA AGGATGCCCC AGGTGAGGAT GAGGAGGAAG 
     2881 ATGGTGTCAG TGAAGCGGCC AGCCTAGAGG AGCCTAAGGA AGAGGATCAA GGAGAAGGCT 
     2941 ACTTGTCAGA AATGGATAAT GAACCTCCTG TGAGCGAGAG TGATGATGGC TTCAGCATAC 
     3001 ACAATGCTAC ACTGCAGTCA CACACACTGG CAGACTCCAT CCCCAGCAGC CCTGCTTCTT 
     3061 CACAGTTCTC TGTCTGTAGT GAGGATCAGG AAGCTATTCA GGCACAGAAA ATTTGGAAGA 
     3121 AAGCCATCAT GCTTGTATGG AGAGCTGCAG CTAATCATAG GTATGCCAAT GTCTTCCTGC 
     3181 AGCCTGTTAC AGATGACATA GCACCTGGCT ACCACAGCAT TGTGCAGAGG CCTATGGATT 
     3241 TGTCAACTAT TAAGAAAAAC ATAGAAAATG GACTGATCCG AAGCACAGCT GAATTTCAGC 
     3301 GTGACATTAT GCTGATGTTT CAGAATGCTG TAATGTACAA TAGCTCAGAC CATGATGTCT 
     3361 ATCACATGGC AGTGGAGATG CAGCGAGATG TCTTGGAACA GATCCAGCAA TTCTTGGCCA 
     3421 CGCAGTTGAT TATGCAAACA TCCGAGTCTG GGATCAGTGC TAAAAGTCTT CGAGGGAGAG 
     3481 ATTCTACCCG CAAACAGGAT GCTTCAGAGA AGGACAGTGT CCCAATGGGC TCTCCTGCCT 
     3541 TCCTTCTCTC TCTCTTTGAT GGAGGAACCA GGGGACGCCG CTGTGCCATT GAAGCAGATA 
     3601 TGAAGATGAA AAAGACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3661 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3721 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3781 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3841 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3901 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     3961 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4021 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4081 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4141 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4201 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4261 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4321 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5041 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5101 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5161 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5221 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5281 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5341 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5401 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5461 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5521 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5581 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5641 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5701 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5761 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5821 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5881 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     5941 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6001 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6061 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6121 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6181 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6241 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6301 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6361 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6421 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6481 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6541 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6601 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6661 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6721 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6781 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6841 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6901 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     6961 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7021 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7081 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7141 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7201 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7261 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7321 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7381 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7441 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7501 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7561 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7621 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7681 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7741 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7801 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7861 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7921 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     7981 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8041 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8101 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8161 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8221 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8281 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8341 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8401 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8461 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8521 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8581 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8641 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8701 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8761 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8821 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8881 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     8941 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9001 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9061 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9121 TTTAACAAAA ATTTAACGCG AATTTT
//