LOCUS       RG239699                9140 bp    DNA     circular  SYN 
DEFINITION  HSPH1 (tGFP-tagged) - Human heat shock 105kDa/110kDa protein 1 (HSPH1), transcript variant 3
ACCESSION   NM_001286504
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3621..4325 
                     /label="GFP"  
     misc_feature    4372..4957 
                     /label="PolyA signal"  
     misc_feature    7049..7694 
                     /label="ColE1"  
     misc_feature    5411..6205 
                     /label="Neo"  
     misc_feature    5042..5344 
                     /label="SV40 ori"  
     misc_feature    7709..8569 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3600..3606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3600 
                     /label="HSPH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAACGGCG GCCGTTCTCC GGGGACCGGC TGCCCATTGG GTAGAATCTT 
     1081 TCCAGAAGGC TCGAGAAGAA GGAAGCGGAA GTGGCACGTG GAGGGGCCGG TGGAGGCGCC 
     1141 GGTCAGTCAT ATCATTTGGA TCAAAAAATA GAACAATCGG AGTTGCAGCC AAAAATCAGC 
     1201 AAATCACTCA TGCAAACAAT ACGGTGTCTA ACTTCAAAAG ATTTCATGGC CGAGCATTCA 
     1261 ATGACCCCTT CATTCAAAAG GAGAAGGAAA ACTTGAGTTA CGATTTGGTT CCATTGAAAA 
     1321 ATGGTGGAGT TGGAATAAAG GTAATGTACA TGGGTGAAGA ACATCTATTT AGTGTGGAGC 
     1381 AGATAACAGC CATGTTGTTG ACTAAGCTGA AGGAAACTGC TGAAAACAGC CTCAAGAAAC 
     1441 CAGTAACAGA TTGTGTTATT TCAGTCCCCT CCTTCTTTAC AGATGCTGAG AGGCGATCTG 
     1501 TGTTAGATGC TGCACAGATT GTTGGCCTAA ACTGTTTAAG ACTTATGAAT GACATGACAG 
     1561 CTGTTGCTTT GAATTACGGA ATTTATAAGC AGGATCTCCC AAGCCTGGAT GAGAAACCTC 
     1621 GGATAGTGGT TTTTGTTGAT ATGGGACATT CAGCTTTTCA AGTGTCTGCT TGTGCTTTTA 
     1681 ACAAGGGAAA ATTGAAGGTA CTGGGAACAG CTTTTGATCC TTTCTTAGGA GGAAAAAACT 
     1741 TCGATGAAAA GTTAGTGGAA CATTTTTGTG CAGAATTTAA AACTAAGTAC AAGTTGGATG 
     1801 CAAAATCCAA AATACGAGCA CTCCTACGTC TGTATCAGGA ATGTGAAAAA CTGAAAAAGC 
     1861 TAATGAGCTC TAACAGCACA GACCTTCCAC TGAATATCGA ATGCTTTATG AATGATAAAG 
     1921 ATGTTTCCGG AAAGATGAAC AGGTCACAAT TTGAAGAACT CTGTGCTGAA CTTCTGCAAA 
     1981 AGATAGAAGT ACCCCTTTAT TCACTGTTGG AACAAACTCA TCTCAAAGTA GAAGATGTGA 
     2041 GTGCAGTTGA GATTGTTGGA GGCGCTACAC GAATTCCAGC TGTGAAGGAA AGAATTGCCA 
     2101 AATTCTTTGG AAAAGATATT AGCACAACAC TCAATGCAGA TGAAGCAGTA GCCAGAGGAT 
     2161 GTGCATTACA GTGTGCAATA CTTTCCCCGG CATTTAAAGT TAGAGAATTT TCCGTCACAG 
     2221 ATGCAGTTCC TTTTCCAATA TCTCTGATCT GGAACCATGA TTCAGAAGAT ACTGAAGGTG 
     2281 TTCATGAAGT CTTTAGTCGA AACCATGCTG CTCCTTTCTC CAAAGTTCTC ACCTTTCTGA 
     2341 GAAGGGGGCC TTTTGAGCTA GAAGCTTTCT ATTCTGATCC CCAAGGAGTT CCATATCCAG 
     2401 AAGCAAAAAT AGGCCGCTTT GTAGTTCAGA ATGTTTCTGC ACAGAAAGAT GGAGAAAAAT 
     2461 CTAGAGTAAA AGTCAAAGTG CGAGTCAACA CCCATGGCAT TTTCACCATC TCTACGGCAT 
     2521 CTATGGTGGA GAAAGTCCCA ACTGAGGAGA ATGAAATGTC TTCTGAAGCT GACATGGAGT 
     2581 GTCTGAATCA GAGACCACCA GAAAACCCAG ACACTGATAA AAATGTCCAG CAAGACAACA 
     2641 GTGAAGCTGG AACACAGCCC CAGGTACAAA CTGATGCTCA ACAAACCTCA CAGTCTCCCC 
     2701 CTTCACCTGA ACTTACCTCA GAAGAAAACA AAATCCCAGA TGCTGACAAA GCAAATGAAA 
     2761 AAAAAGTTGA CCAGCCTCCA GAAGCTAAAA AGCCCAAAAT AAAGGTGGTG AATGTTGAGC 
     2821 TGCCTATTGA AGCCAACTTG GTCTGGCAGT TAGGGAAAGA CCTTCTTAAC ATGTATATTG 
     2881 AGACAGAGGG TAAGATGATA ATGCAAGATA AATTGGAAAA AGAAAGGAAT GATGCTAAAA 
     2941 ATGCAGTTGA GGAATATGTG TATGAGTTCA GAGACAAGCT GTGTGGACCA TATGAAAAAT 
     3001 TTATATGTGA GCAGGATCAT CAAAATTTTT TGAGACTCCT CACAGAAACT GAAGACTGGC 
     3061 TGTATGAAGA AGGAGAGGAC CAAGCTAAAC AAGCATATGT TGACAAGTTG GAAGAATTAA 
     3121 TGAAAATTGG CACTCCAGTT AAAGTTCGGT TTCAGGAAGC TGAAGAACGG CCAAAAATGT 
     3181 TTGAAGAACT AGGACAGAGG CTGCAGCATT ATGCCAAGAT AGCAGCTGAC TTCAGAAATA 
     3241 AGGATGAGAA ATACAACCAT ATTGATGAGT CTGAAATGAA AAAAGTGGAG AAGTCTGTTA 
     3301 ATGAAGTGAT GGAATGGATG AATAATGTCA TGAATGCTCA GGCTAAAAAG AGTCTTGATC 
     3361 AGGATCCAGT TGTACGTGCT CAGGAAATTA AAACAAAAAT CAAGGAATTG AACAACACAT 
     3421 GTGAACCCGT TGTAACACAA CCGAAACCAA AAATTGAATC ACCCAAACTG GAAAGAACTC 
     3481 CAAATGGCCC AAATATTGAT AAAAAGGAAG AAGATTTAGA AGACAAAAAC AATTTTGGTG 
     3541 CTGAACCTCC ACATCAGAAT GGTGAATGTT ACCCTAATGA GAAAAATTCT GTTAATATGG 
     3601 ACTTGGACAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3661 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3721 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3781 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3841 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3901 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     3961 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4021 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4081 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4141 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4201 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4261 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4321 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4381 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4441 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4501 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4561 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4621 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4681 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4741 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4801 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4861 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4921 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4981 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5041 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5101 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5221 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5281 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5341 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5401 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5461 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5521 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5581 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5641 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5701 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5761 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5821 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5881 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     5941 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6001 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6061 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6121 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6181 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6241 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6301 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6361 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6421 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6481 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6541 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6601 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6661 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6721 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6781 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6841 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6901 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     6961 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7021 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7081 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7141 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7201 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7261 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7321 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7381 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7441 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7501 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7561 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7621 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7681 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7741 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7801 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7861 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7921 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     7981 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8041 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8101 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8161 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8221 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8281 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8341 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8401 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8461 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8521 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8581 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8641 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8701 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8761 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8821 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8881 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     8941 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9001 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9061 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9121 AAAAATTTAA CGCGAATTTT 
//