LOCUS       RG239694                9134 bp    DNA     circular  SYN 
DEFINITION  RAPGEF4 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 3
ACCESSION   NM_001282899
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3615..4319 
                     /label="GFP"  
     misc_feature    4366..4951 
                     /label="PolyA signal"  
     misc_feature    7043..7688 
                     /label="ColE1"  
     misc_feature    5405..6199 
                     /label="Neo"  
     misc_feature    5036..5338 
                     /label="SV40 ori"  
     misc_feature    7703..8563 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3594..3600 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3594 
                     /label="RAPGEF4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGACTT CTGGCTCCTC CTTATGGTGT TATGGAAACG GGCTCTAACA 
     1081 ATGACAGGAT TCCTGACAAG GAGAACACAC CTCTCATTGA ACCTCACGTT CCTCTTCGTC 
     1141 CTGCTAACAC CATTACCAAG GTCCCTTCAG AGAAGATCCT CAGAGCTGGA AAAATTTTAC 
     1201 GAAATGCCAT TCTCTCTCGA GCACCTCACA TGATAAGAGA TAGAAAATAC CACCTAAAGA 
     1261 CATACAGACA ATGCTGTGTG GGAACTGAAC TGGTGGACTG GATGATGCAG CAGACACCAT 
     1321 GTGTTCACTC CCGGACTCAA GCTGTTGGCA TGTGGCAAGT CCTGTTAGAA GATGGTGTTC 
     1381 TCAACCACGT GGACCAGGAG CACCATTTCC AAGACAAATA TTTATTCTAT CGATTTCTGG 
     1441 ATGATGAGCA CGAGGATGCC CCTTTGCCTA CTGAGGAGGA GAAGAAGGAG TGTGATGAGG 
     1501 AGCTCCAGGA CACCATGCTG CTGCTGTCAC AGATGGGCCC CGACGCCCAC ATGAGGATGA 
     1561 TCCTTCGCAA ACCACCTGGC CAGAGGACTG TGGATGACCT AGAGATTATC TATGAGGAGC 
     1621 TTCTTCATAT TAAAGCCTTA TCCCATCTTT CTACCACAGT GAAACGAGAG TTAGCAGGTG 
     1681 TTCTCATTTT TGAGTCTCAC GCCAAAGGAG GGACTGTGTT GTTTAACCAG GGGGAAGAAG 
     1741 GTACCTCCTG GTACATTATT CTAAAAGGAT CAGTGAATGT AGTCATTTAC GGCAAGGGTG 
     1801 TGGTCTGCAC CCTGCATGAA GGAGATGACT TCGGCAAGTT AGCACTAGTG AATGATGCCC 
     1861 CACGAGCTGC CTCTATCGTC TTACGAGAAG ATAACTGCCA TTTCTTAAGA GTAGACAAGG 
     1921 AGGATTTCAA CCGGATCCTA AGGGACGTGG AGGCGAATAC AGTCAGACTT AAAGAACATG 
     1981 ACCAAGATGT CTTGGTGCTG GAGAAGGTCC CAGCAGGGAA CAGAGCTTCT AATCAAGGAA 
     2041 ACTCACAGCC TCAGCAAAAG TATACTGTGA TGTCAGGAAC ACCTGAAAAA ATTTTAGAGC 
     2101 ATTTTCTAGA AACAATACGC CTTGAGGCAA CTTTAAATGA AGCAACAGAT TCTGTTTTAA 
     2161 ATGACTTTAT TATGATGCAC TGTGTTTTTA TGCCAAATAC CCAGCTTTGC CCGGCACTGG 
     2221 TGGCCCACTA CCACGCACAG CCTTCACAAG GTACAGAACA GGAGAAAATG GATTATGCCC 
     2281 TCAACAATAA GAGGCGAGTC ATCCGCCTGG TTCTACAGTG GGCTGCCATG TATGGAGACC 
     2341 TCCTGCAAGA GGATGACGTG TCTATGGCCT TCCTGGAGGA GTTTTATGTA TCTGTATCAG 
     2401 ATGATGCCCG GATGATTGCT GCCCTCAAGG AGCAACTGCC AGAGTTGGAG AAGATTGTCA 
     2461 AGCAAATCTC AGAAGATGCA AAGGCACCAC AAAAGAAGCA CAAGGTTCTT TTGCAACAGT 
     2521 TCAATACGGG CGATGAGAGA GCCCAGAAGC GCCAGCCTAT CCGCGGCTCT GATGAAGTTC 
     2581 TGTTTAAGGT CTATTGCATG GACCACACCT ACACAACCAT TCGGGTGCCA GTGGCCACTT 
     2641 CGGTGAAGGA AGTCATCAGT GCAGTTGCCG ACAAGCTGGG CTCCGGGGAG GGCCTGATCA 
     2701 TAGTCAAGAT GAGTTCCGGA GGAGAAAAGG TGGTGCTCAA ACCTAATGAT GTTTCAGTAT 
     2761 TTACGACGCT CACCATTAAT GGACGCCTGT TTGCTTGCCC GCGAGAGCAA TTCGATTCAC 
     2821 TGACTCCCTT ACCAGAACAG GAAGGCCCAA CTGTTGGAAC AGTGGGAACT TTTGAACTGA 
     2881 TGAGCTCCAA AGATTTAGCA TACCAGATGA CAATTTATGA TTGGGAACTC TTCAACTGCG 
     2941 TGCATGAGCT GGAGCTAATC TATCACACAT TTGGAAGGCA TAATTTTAAA AAGACCACAG 
     3001 CAAACTTGGA TTTGTTCCTG AGGAGATTTA ATGAAATTCA GTTTTGGGTC GTCACTGAGA 
     3061 TCTGCCTTTG TTCTCAGCTC AGCAAGCGTG TTCAGCTATT AAAAAAATTT ATTAAGATAG 
     3121 CAGCCCACTG TAAGGAGTAT AAAAATCTGA ATTCCTTTTT TGCCATCGTC ATGGGACTAA 
     3181 GTAACGTTGC TGTGAGCCGC TTGGCACTAA CGTGGGAGAA ACTGCCAAGC AAGTTCAAGA 
     3241 AGTTCTATGC GGAGTTTGAA AGTTTAATGG ACCCTTCAAG GAACCACAGG GCCTACAGGC 
     3301 TGACAGTAGC TAAGCTGGAA CCTCCTCTCA TCCCCTTCAT GCCTTTGCTC ATTAAAGATA 
     3361 TGACATTTAC TCATGAGGGG AACAAGACGT TCATTGACAA TCTAGTAAAC TTTGAAAAAA 
     3421 TGCGCATGAT TGCAAATACG GCCAGAACAG TGAGATACTA CAGGAGCCAA CCCTTCAATC 
     3481 CTGATGCAGC TCAAGCTAAT AAGAACCATC AGGATGTCCG GAGTTATGTA CGGCAATTAA 
     3541 ATGTGATTGA CAACCAGAGA ACTTTATCAC AGATGTCACA CAGATTAGAG CCTCGTCGAC 
     3601 CAACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3661 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3721 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     3781 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     3841 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     3901 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     3961 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4021 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4081 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4141 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4201 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4261 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4321 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4381 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4441 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4501 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4561 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4621 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4681 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4741 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4801 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4861 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4921 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4981 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5041 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5101 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5161 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5221 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5281 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5341 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5401 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5461 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5521 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5581 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5641 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5701 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     5761 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     5821 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     5881 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     5941 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6001 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6061 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6121 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6181 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6241 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6301 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6361 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6421 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6481 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6541 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6601 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6661 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6721 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     6781 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     6841 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     6901 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     6961 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7021 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7081 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7141 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7201 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7261 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7321 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7381 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7441 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7501 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7561 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7621 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7681 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7741 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     7801 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     7861 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     7921 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     7981 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8041 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8101 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8161 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8221 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8281 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8341 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8401 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8461 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8521 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8581 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8641 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8701 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     8761 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     8821 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     8881 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     8941 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9001 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9061 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9121 TTAACGCGAA TTTT
//