LOCUS       RG239371                8678 bp    DNA     circular  SYN 
DEFINITION  MRVI1 (tGFP-tagged) - Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 5
ACCESSION   NM_001206880
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3159..3863 
                     /label="GFP"  
     misc_feature    3910..4495 
                     /label="PolyA signal"  
     misc_feature    6587..7232 
                     /label="ColE1"  
     misc_feature    4949..5743 
                     /label="Neo"  
     misc_feature    4580..4882 
                     /label="SV40 ori"  
     misc_feature    7247..8107 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3138..3144 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3138 
                     /label="IRAG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAAAAGCT CCCCAGAGTG AAGAGAGGCT GGCCAGAGGA GGAAAGGAGA 
     1081 ATAACTCAGT TTTAGCCTGT GGAGCCCAGG CCTCTTGGAG CATCTTTGGG GCTGACGCAG 
     1141 CGGAGGTTCC GGGCACACGT GGCCACTCCC AGCAGGAGGC TGCCATGCCC CACATTCCCG 
     1201 AGGACGAGGA GCCCCCCGGA GAGCCACAGG CAGCCCAGAG CCCTGCCGGC CAAGGTCCTC 
     1261 CTGCCGCAGG AGTATCTTGC AGTCCAACTC CCACGATTGT CCTGACTGGG GATGCCACTT 
     1321 CACCAGAAGG AGAAACCGAC AAAAACCTGG CCAACAGAGT TCACAGTCCC CACAAGAGGC 
     1381 TTTCTCACCG ACACTTGAAG GTGTCCACTG CCTCCCTGAC ATCTGTGGAC CCCGCGGGGC 
     1441 ACATCATTGA CCTGGTGAAT GACCAGCTGC CAGACATCAG CATCTCAGAG GAGGACAAGA 
     1501 AGAAAAACCT GGCGCTGCTG GAAGAAGCCA AGTTGGTGAG TGAGCGATTC CTGACCCGCC 
     1561 GTGGGAGGAA GTCCAGGAGC AGCCCCGGAG ACTCCCCATC AGCTGTTTCC CCGAACCTCA 
     1621 GCCCCAGCGC TTCTCCTACA TCCTCTCGGA GCAACTCACT TACAGTCCCC ACCCCGCCAG 
     1681 GTTTGGATGT GTGCAGTGGC CCGCCATCCC CTCTGCCTGG AGCACCACCA CAGAAGGGGG 
     1741 ATGAGGCCGA CGTCTCTTCA CCTCACCCTG GCGAGCCTGA GCACATCCTG ATGAGAAATC 
     1801 AGAACTTAGT GGGGCTCAAG CTTCCAGACC TTAGTGAAGC AGCTGAGCAG GAAAAAGGGC 
     1861 TTCCTTCTGA ACTCTCCCCA GCTATTGAGG AAGAAGAGTC AAAGAGTGGC TTAGATGTCA 
     1921 TGCCTAATAT TTCTGATGTG CTGCTGCGCA AACTGCGGGT CCACAGGAGT CTCCCTGGAA 
     1981 GTGCCCCTCC ACTCACTGAA AAGGAAGTTG AGAACGTGTT TGTGCAACTG TCCTTGGCCT 
     2041 TTAGAAATGA CAGCTACACT CTGGAATCTA GAATTAACCA GGCTGAAAGG GAACGCAACC 
     2101 TGACAGAGGA GAACACTGAG AAAGAACTGG AAAACTTCAA AGCTTCCATT ACGTCCTCAG 
     2161 CTTCACTCTG GCACCACTGT GAGCACCGGG AAACCTACCA GAAGTTGCTG GAGGACATCG 
     2221 CTGTCCTGCA CCGCCTGGCT GCCCGCCTCT CCAGCCGAGC TGAGGTGGTA GGCGCCGTCC 
     2281 GCCAGGAAAA GCGCATGTCG AAAGCAACGG AAGTGATGAT GCAGTATGTG GAGAATCTAA 
     2341 AGAGGACGTA TGAGAAGGAC CATGCGGAGC TCATGGAGTT TAAAAAGCTT GCAAATCAGA 
     2401 ATTCAAGCCG CAGCTGTGGC CCCTCTGAAG ATGGGGTCCC TCGCACGGCA CGGTCCATGT 
     2461 CCCTCACGCT GGGAAAGAAT ATGCCTCGCC GGAGGGTCAG CGTTGCTGTG GTTCCTAAGT 
     2521 TTAATGCCCT GAATCTGCCT GGCCAAACTC CCAGCTCATC ATCCATTCCC TCCTTACCAG 
     2581 CCTTGTCGGA ATCACCCAAT GGGAAAGGCA GCCTACCTGT CACTTCAGCA CTGCCTGCAC 
     2641 TTTTGGAAAA TGGAAAGACA AATGGGGACC CAGATTGTGA AGCCTCTGCT CCTGCGCTGA 
     2701 CCCTGAGCTG CCTGGAGGAG CTTAGTCAGG AGACCAAGGC CAGGATGGAG GAAGAAGCCT 
     2761 ACAGCAAGGG ATTCCAAGAA GGTCTAAAGA AGACCAAAGA ACTTCAAGAC CTGAAGGAGG 
     2821 AGGAGGAAGA ACAGAAGAGT GAGAGTCCTG AGGAACCTGA AGAGGTAGAA GAAACTGAGG 
     2881 AAGAGGAAAA GGGCCCAAGA AGCAGCAAAC TTGAAGAATT GGTCCATTTC TTACAAGTCA 
     2941 TGTATCCCAA ACTGTGTCAG CACTGGCAAG TGATCTGGAT GATGGCTGCA GTGATGCTGG 
     3001 TCTTGACTGT TGTGCTGGGG CTCTACAATT CCTATAACTC TTGTGCAGAG CAGGCTGATG 
     3061 GGCCCCTTGG AAGATCCACT TGCTCGGCAG CCCAGAGGGA CTCCTGGTGG AGCTCAGGAC 
     3121 TCCAGCATGA GCAGCCTACA GAGCAGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3181 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3241 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3301 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3361 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3421 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3481 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3541 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     3601 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     3661 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     3721 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     3781 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     3841 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3901 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3961 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4021 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4081 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4141 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4201 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4261 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4321 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4381 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4441 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4501 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4561 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4621 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4681 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     4741 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     4801 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     4861 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     4921 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     4981 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5041 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5101 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5161 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5221 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5281 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5341 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5401 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5461 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5521 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     5581 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     5641 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     5701 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     5761 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     5821 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     5881 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     5941 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6001 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6061 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6121 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6181 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6241 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6301 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6361 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6421 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6481 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6541 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     6601 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     6661 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     6721 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     6781 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     6841 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     6901 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     6961 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7021 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7081 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7141 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7201 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7261 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7321 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7381 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7441 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7501 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7561 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     7621 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     7681 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     7741 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     7801 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     7861 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     7921 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     7981 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8041 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8101 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8161 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8221 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8281 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8341 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8401 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8461 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8521 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     8581 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     8641 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//