LOCUS       RG239349                8660 bp    DNA     circular  SYN 
DEFINITION  HELLS (tGFP-tagged) - Human helicase, lymphoid-specific (HELLS), transcript variant 8
ACCESSION   NM_001289073
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3141..3845 
                     /label="GFP"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    6569..7214 
                     /label="ColE1"  
     misc_feature    4931..5725 
                     /label="Neo"  
     misc_feature    4562..4864 
                     /label="SV40 ori"  
     misc_feature    7229..8089 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3120..3126 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3120 
                     /label="HELLS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAAGTGAAG AGAAGCCAGG AAATTTTGTC TGTGGCTAAA AAAAATAAAA 
     1081 AGGAGAATGA GGATGAAAAC TCCTCCTCTA CTAATCTCTG TGTGGAAGAT CTTCAGAAAA 
     1141 ATAAAGATTC GAATAGTATA ATTAAAGATA GATTGTCTGA AACGGTTAGG CAGAATACTA 
     1201 AATTCTTTTT TGACCCAGTC CGGAAGTGTA ATGGTCAGCC AGTACCTTTT CAACAACCAA 
     1261 AGCACTTCAC TGGAGGAGTG ATGCGATGGT ACCAAGTAGA AGGCATGGAA TGGCTTAGGA 
     1321 TGCTTTGGGA AAATGGAATT AATGGCATTT TAGCAGATGA AATGGGATTG GGTAAGACAG 
     1381 TTCAGTGCAT TGCTACTATT GCATTGATGA TTCAGAGAGG AGTACCAGGA CCTTTTCTTG 
     1441 TCTGTGGCCC TTTGTCTACA CTTCCTAACT GGATGGCTGA ATTCAAAAGA TTTACACCAG 
     1501 ATATCCCTAC AATGTTATAT CATGGAACCC AGGAGGAACG TCAAAAATTG GTAAGAAATA 
     1561 TTTACAAACG GAAAGGGACT TTGCAGATTC ATCCTGTGGT AATCACGTCA TTTGAAATAG 
     1621 CCATGAGAGA CCGAAATGCG TTACAGCATT GCTATTGGAA ATACTTAATA GTAGATGAAG 
     1681 GACACAGGAT TAAGAATATG AAGTGCCGTC TAATCAGGGA GTTAAAACGA TTCAATGCTG 
     1741 ATAACAAACT TCTTTTGACT GGTACTCCCT TGCAAAACAA TTTATCAGAA CTTTGGTCAT 
     1801 TGCTAAACTT TTTGTTGCCA GATGTATTTG ATGACTTGAA AAGCTTTGAG TCTTGGTTTG 
     1861 ACATCACTAG TCTTTCTGAA ACTGCTGAAG ATATTATTGC TAAAGAAAGA GAACAGAATG 
     1921 TATTGCATAT GCTGCACCAG ATTTTAACAC CTTTCTTATT GAGAAGACTG AAGTCTGATG 
     1981 TTGCTCTTGA AGTTCCTCCT AAACGAGAAG TAGTCGTTTA TGCTCCACTT TCAAAGAAGC 
     2041 AGGAGATCTT TTATACAGCC ATTGTGAACC GTACAATTGC AAACATGTTT GGATCCAGTG 
     2101 AGAAAGAAAC AATTGAGTTA AGTCCTACTG GTCGACCAAA ACGACGAACT AGAAAATCAA 
     2161 TAAATTACAG CAAAATAGAT GATTTCCCTA ATGAATTGGA AAAACTGATC AGTCAAATAC 
     2221 AGCCAGAGGT GGACCGAGAA AGAGCTGTTG TGGAAGTGAA TATCCCTGTA GAATCTGAAG 
     2281 TTAATCTGAA GCTGCAGAAT ATAATGATGC TACTTCGTAA ATGTTGTAAT CATCCATATT 
     2341 TGATTGAATA TCCTATAGAC CCTGTTACAC AAGAATTTAA GATCGATGAA GAATTGGTAA 
     2401 CAAATTCTGG GAAGTTCTTG ATTTTGGATC GAATGCTGCC AGAACTAAAA AAAAGAGGTC 
     2461 ACAAGGTGCT GCTTTTTTCA CAAATGACAA GCATGTTGGA CATTTTGATG GATTACTGCC 
     2521 ATCTCAGAGA TTTCAACTTC AGCAGGCTTG ATGGGTCCAT GTCTTACTCA GAGAGAGAAA 
     2581 AAAACATGCA CAGCTTCAAC ACGGATCCAG AGGTGTTTAT CTTCTTAGTG AGTACACGAG 
     2641 CTGGTGGCCT GGGCATTAAT CTGACTGCAG CAGATACAGT TATCATTTAT GATAGTGATT 
     2701 GGAACCCCCA GTCGGATCTT CAGGCCCAGG ATAGATGTCA TAGAATTGGT CAGACAAAGC 
     2761 CAGTTGTTGT TTATCGCCTT GTTACAGCAA ATACTATCGA TCAGAAAATT GTGGAAAGAG 
     2821 CAGCTGCTAA AAGGAAACTG GAAAAGTTGA TCATCCATAA AAATCATTTC AAAGGTGGTC 
     2881 AGTCTGGATT AAATCTGTCT AAGAATTTCT TAGATCCTAA GGAATTAATG GAATTATTAA 
     2941 AATCTAGAGA TTATGAAAGG GAAATAAAAG GATCAAGAGA GAAGGTCATT AGTGATAAAG 
     3001 ATCTAGAGTT GTTGTTAGAT CGAAGTGATC TTATTGATCA AATGAATGCT TCAGGACCAA 
     3061 TTAAAGAGAA GATGGGGATA TTCAAGATAT TAGAAAATTC TGAAGATTCC AGTCCTGAAT 
     3121 GTTTGTTTAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3181 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3241 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3301 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3361 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3421 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     3481 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     3541 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     3601 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     3661 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     3721 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     3781 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     3841 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3901 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3961 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4021 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4081 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4141 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4201 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4261 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4321 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4381 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4441 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4501 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     4561 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4621 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     4681 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     4741 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     4801 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     4861 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     4921 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     4981 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5041 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5101 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5161 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5221 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5281 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5341 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5401 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     5461 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     5521 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     5581 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     5641 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     5701 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     5761 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     5821 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     5881 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     5941 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6001 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6061 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6121 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6181 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6241 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6301 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6361 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6421 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     6481 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     6541 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     6601 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     6661 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     6721 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     6781 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     6841 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     6901 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     6961 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7021 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7081 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7141 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7201 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7261 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7321 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7381 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7441 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     7501 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     7561 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     7621 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     7681 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     7741 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     7801 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     7861 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     7921 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     7981 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8041 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8101 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8161 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8221 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8281 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8341 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8401 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     8461 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     8521 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     8581 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     8641 AAAAATTTAA CGCGAATTTT 
//