LOCUS       RG235111                9548 bp    DNA     circular  SYN 
DEFINITION  STK31 (tGFP-tagged) - Homo sapiens serine/threonine kinase 31 (STK31), transcript variant 4
ACCESSION   NM_001260504
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4029..4733 
                     /label="GFP"  
     misc_feature    4780..5365 
                     /label="PolyA signal"  
     misc_feature    7457..8102 
                     /label="ColE1"  
     misc_feature    5819..6613 
                     /label="Neo"  
     misc_feature    5450..5752 
                     /label="SV40 ori"  
     misc_feature    8117..8977 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4008..4014 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4008 
                     /label="STK31"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAAGAT ACACATTACG ATAAAGTGGA AGATGTGGTT GGAAGTCACA 
     1081 TAGAAGATGC AGTAACATTT TGGGCCCAGA GTATCAATAG AAATAAGGAT ATCATGAAGA 
     1141 TTGGTTGCTC ACTGTCTGAA GTTTGCCCCC AGGCCAGTTC AGTTTTGGGG AATCTTGACC 
     1201 CAAACAAGAT TTATGGTGGA TTATTTTCTG AAGATCAGTG TTGGTACAGA TGCAAAGTAC 
     1261 TGAAAATCAT CAGCGTTGAA AAGTGTCTGG TGAGGTACAT TGACTATGGA AATACTGAAA 
     1321 TTCTAAATCG ATCTGATATA GTTGAAATTC CTTTGGAGCT GCAGTTTTCT AGTGTTGCCA 
     1381 AAAAGTATAA ACTTTGGGGA CTACACATTC CTTCTGATCA AGAAGTTACC CAGTTTGATC 
     1441 AGGGCACAAC CTTTTTGGGG AGCTTGATTT TTGAAAAGGA AATAAAAATG AGAATTAAAG 
     1501 CAACCTCTGA AGATGGAACA GTTATTGCTC AGGCTGAGTA TGGCAGTGTG GATATAGGGG 
     1561 AAGAGGTGCT TAAGAAAGGA TTTGCAGAGA AATGCAGACT TGCTTCCAGA ACTGACATCT 
     1621 GTGAGGAAAA AAAATTGGAT CCTGGTCAAC TTGTTCTCAG GAACCTCAAA AGCCCCATTC 
     1681 CTTTGTGGGG GCATAGATCA AACCAGTCAA CCTTCAGCAG GCCCAAGGGG CACTTAAGTG 
     1741 AGAAAATGAC TCTTGACTTG AAGGATGAAA ATGATGCAGG CAATCTTATA ACATTTCCAA 
     1801 AGGAAAGTTT GGCTGTTGGT GACTTTAATT TAGGGTCTAA CGTCAGCCTG GAAAAAATTA 
     1861 AGCAGGACCA GAAACTGATT GAAGAAAATG AAAAACTTAA AACAGAGAAG GACGCTCTTC 
     1921 TTGAAAGTTA TAAGGCGTTA GAATTGAAAG TAGAGCAGAT TGCCCAGGAG CTGCAGCAAG 
     1981 AGAAGGCAGC TGCTGTGGAT TTGACTAACC ACTTAGAATA CACTCTGAAG ACCTATATAG 
     2041 ATACCAGAAT GAAAAATCTG GCAGCTAAGA TGGAAATACT GAAAGAAATG AGGCATGTCG 
     2101 ACATCAGTGT CCGTTTCGGA AAAGACCTTT CAGATGCTAT ACAAGTGTTG GATGAAGGGT 
     2161 GCTTTACTAC TCCAGCTTCT TTGAATGGAT TAGAGATAAT ATGGGCAGAA TACAGTCTGG 
     2221 CTCAGGAGAA TATTAAAACT TGTGAATATG TGAGTGAAGG GAATATTTTG ATTGCCCAAA 
     2281 GAAATGAAAT GCAGCAGAAG CTGTACATGT CAGTAGAAGA TTTTATTCTG GAAGTTGATG 
     2341 AGTCATCTCT TAATAAACGC TTAAAAACAT TGCAGGATTT GTCAGTCTCT TTAGAAGCAG 
     2401 TGTATGGACA AGCCAAAGAA GGAGCAAATT CTGATGAAAT ACTTAAAAAA TTTTATGACT 
     2461 GGAAGTGTGA TAAAAGAGAG GAGTTCACCA GTGTTAGAAG TGAAACAGAC GCTTCTCTGC 
     2521 ACCGTCTTGT AGCATGGTTC CAAAGAACCT TAAAGGTTTT TGACCTATCT GTGGAAGGAT 
     2581 CACTGATTTC AGAAGACGCA ATGGATAATA TTGATGAAAT CCTAGAGAAG ACTGAGTCAA 
     2641 GTGTCTGCAA AGAGCTGGAG ATAGCTCTGG TTGATCAAGG TGATGCAGAC AAGGAGATAA 
     2701 TTTCAAATAC ATATAGTCAA GTACTGCAAA AGATTCATTC AGAGGAAAGG CTCATTGCCA 
     2761 CAGTACAAGC TAAGTACAAG GACAGTATTG AGTTTAAAAA GCAGCTTATT GAATATTTAA 
     2821 ATAAGAGTCC CAGTGTGGAT CACTTGCTAT CCATTAAGAA GACATTGAAA AGCTTAAAAG 
     2881 CTCTACTCAG ATGGAAATTG GTTGAAAAGA GTAATTTGGA AGAGTCAGAT GATCCTGATG 
     2941 GCTCTCAAAT TGAGAAAATA AAAGAAGAAA TAACTCAGCT GCGCAATAAT GTCTTTCAGG 
     3001 AAATTTATCA TGAGAGAGAG GAATATGAGA TGCTAACTAG TTTGGCACAG AAATGGTTCC 
     3061 CTGAGCTGCC TCTGCTTCAT CCTGAAATAG GATTACTCAA ATACATGAAC TCTGGTGGTC 
     3121 TCCTTACAAT GAGCTTGGAA CGAGATCTTC TTGATGCTGA GCCCATGAAG GAACTTAGCA 
     3181 GCAAGCGTCC TTTGGTACGT TCTGAGGTTA ATGGGCAGAT AATTCTGTTA AAGGGCTATT 
     3241 CTGTGGATGT TGACACAGAA GCCAAGGTGA TTGAGAGAGC AGCCACCTAC CATAGAGCTT 
     3301 GGAGAGAAGC TGAAGGAGAC TCAGGGTTAC TGCCATTGAT ATTCCTGTTT TTATGTAAGT 
     3361 CTGATCCTAT GGCTTATCTG ATGGTCCCAT ACTACCCTAG GGCAAACCTG AATGCTGTTC 
     3421 AAGCCAACAT GCCTTTAAAT TCAGAAGAAA CTTTAAAGGT CATGAAAGGT GTTGCCCAGG 
     3481 GTCTGCATAC ATTGCATAAG GCTGACATAA TTCATGGATC ACTTCATCAG AACAATGTAT 
     3541 TTGCTTTAAA CCGTGAACAA GGAATTGTTG GAGATTTTGA CTTCACCAAA TCTGTGAGTC 
     3601 AGCGAGCCTC GGTGAACATG ATGGTTGGTG ACTTGAGTTT GATGTCACCT GAGTTGAAAA 
     3661 TGGGAAAACC TGCTTCTCCA GGTTCAGACT TATATGCTTA TGGCTGCCTC TTATTATGGC 
     3721 TTTCTGTTCA AAATCAGGAG TTTGAGATAA ATAAAGATGG AATCCCCAAA GTGGATCAGT 
     3781 TTCATCTGGA TGATAAAGTC AAATCCCTCC TCTGTAGCTT GATATGTTAT AGAAGTTCAA 
     3841 TGACTGCTGA ACAAGTTTTA AATGCTGAAT GTTTCTTGAT GCCAAAGGAG CAATCAGTTC 
     3901 CAAACCCAGA AAAAGATACT GAATACACCC TATATAAAAA GGAAGAAGAA ATAAAGACGG 
     3961 AGAACTTGGA TAAATGTATG GAGAAGACAA GAAATGGTGA AGCCAACTTT GATTGTACGC 
     4021 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     4081 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4141 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4201 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4261 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4321 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4381 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4441 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4501 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4561 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4621 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4681 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4741 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4801 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4861 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4921 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4981 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5041 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5101 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5161 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5221 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5281 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5341 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5401 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5461 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5521 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5581 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5641 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5701 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5761 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5821 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5881 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5941 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     6001 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     6061 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6121 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6181 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6241 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6301 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6361 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6421 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6481 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6541 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6601 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6661 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6721 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6781 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6841 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6901 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6961 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     7021 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     7081 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7141 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7201 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7261 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7321 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7381 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7441 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7501 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7561 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7621 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7681 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7741 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7801 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7861 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7921 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7981 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     8041 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     8101 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8161 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8221 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8281 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8341 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8401 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8461 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8521 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8581 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8641 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8701 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8761 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8821 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8881 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8941 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     9001 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9061 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9121 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9181 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9241 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9301 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9361 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9421 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9481 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9541 CGAATTTT
//