LOCUS       RG235084                9455 bp    DNA     circular  SYN 
DEFINITION  SLC8A1 (tGFP-tagged) - Homo sapiens solute carrier family 8 (sodium/calcium exchanger), member 1 (SLC8A1), transcript variant E
ACCESSION   NM_001252624
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3936..4640 
                     /label="GFP"  
     misc_feature    4687..5272 
                     /label="PolyA signal"  
     misc_feature    7364..8009 
                     /label="ColE1"  
     misc_feature    5726..6520 
                     /label="Neo"  
     misc_feature    5357..5659 
                     /label="SV40 ori"  
     misc_feature    8024..8884 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3915..3921 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3915 
                     /label="SLC8A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACAACATG CGGCGATTAA GTCTTTCACC CACCTTTTCA ATGGGATTTC 
     1081 ATCTGTTAGT TACTGTGAGT CTCTTATTTT CCCATGTGGA CCATGTAATT GCTGAGACAG 
     1141 AAATGGAAGG AGAAGGAAAT GAAACTGGTG AATGTACTGG ATCATATTAC TGTAAGAAAG 
     1201 GGGTGATTTT GCCCATTTGG GAACCCCAAG ACCCTTCTTT TGGGGACAAA ATTGCTAGAG 
     1261 CTACTGTGTA TTTTGTGGCC ATGGTCTACA TGTTTCTTGG AGTCTCTATC ATAGCTGATC 
     1321 GGTTCATGTC CTCTATAGAA GTCATCACAT CTCAAGAAAA AGAAATAACC ATAAAGAAAC 
     1381 CCAATGGAGA GACCACCAAG ACAACTGTGA GGATCTGGAA TGAAACAGTT TCTAACCTGA 
     1441 CCTTGATGGC CCTGGGATCT TCTGCTCCTG AGATTCTCCT TTCAGTAATT GAAGTGTGTG 
     1501 GCCATAACTT CACTGCAGGA GACCTCGGTC CTAGCACCAT CGTGGGAAGT GCTGCATTCA 
     1561 ATATGTTCAT CATTATTGCA CTCTGTGTTT ATGTGGTGCC TGACGGAGAG ACAAGGAAGA 
     1621 TTAAGCATTT GCGTGTCTTC TTTGTGACAG CAGCCTGGAG CATCTTTGCC TACACCTGGC 
     1681 TTTACATTAT TTTGTCTGTC ATATCTCCTG GTGTTGTGGA GGTCTGGGAA GGTTTGCTTA 
     1741 CTTTCTTCTT CTTTCCCATC TGTGTTGTGT TCGCTTGGGT AGCGGATAGG AGACTTCTGT 
     1801 TTTACAAGTA TGTCTACAAG AGGTATCGAG CTGGCAAGCA GAGGGGGATG ATTATTGAAC 
     1861 ATGAAGGAGA CAGGCCATCT TCTAAGACTG AAATTGAAAT GGACGGGAAA GTGGTCAATT 
     1921 CTCATGTTGA AAATTTCTTA GATGGTGCTC TGGTTCTGGA GGTGGATGAG AGGGACCAAG 
     1981 ATGATGAAGA AGCTAGGCGA GAAATGGCTA GGATTCTGAA GGAACTTAAG CAGAAGCATC 
     2041 CAGATAAAGA AATAGAGCAA TTAATAGAAT TAGCTAACTA CCAAGTCCTA AGTCAGCAGC 
     2101 AAAAAAGTAG AGCATTTTAT CGCATTCAAG CTACTCGCCT CATGACTGGA GCTGGCAACA 
     2161 TTTTAAAGAG GCATGCAGCT GACCAAGCAA GGAAGGCTGT CAGCATGCAC GAGGTCAACA 
     2221 CTGAAGTGAC TGAAAATGAC CCTGTTAGTA AGATCTTCTT TGAACAAGGG ACATATCAGT 
     2281 GTCTGGAGAA CTGTGGTACT GTGGCCCTTA CCATTATCCG CAGAGGTGGT GATTTGACTA 
     2341 ACACTGTGTT TGTTGACTTC AGAACAGAGG ATGGCACAGC AAATGCTGGG TCTGATTATG 
     2401 AATTTACTGA AGGAACTGTG GTGTTTAAGC CTGGTGATAC CCAGAAGGAA ATCAGAGTGG 
     2461 GTATCATAGA TGATGATATC TTTGAGGAGG ATGAAAATTT CCTTGTGCAT CTCAGCAATG 
     2521 TCAAAGTATC TTCTGAAGCT TCAGAAGATG GCATACTGGA AGCCAATCAT GTTTCTACAC 
     2581 TTGCTTGCCT CGGATCTCCC TCCACTGCCA CTGTAACTAT TTTTGATGAT GACCACGCAG 
     2641 GCATTTTTAC TTTTGAGGAA CCTGTGACTC ATGTGAGTGA GAGCATTGGC ATCATGGAGG 
     2701 TGAAAGTATT GAGAACATCT GGAGCTCGAG GAAATGTTAT CGTTCCATAT AAAACCATCG 
     2761 AAGGGACTGC CAGAGGTGGA GGGGAGGATT TTGAGGACAC TTGTGGAGAG CTCGAATTCC 
     2821 AGAATGATGA AATTGTGAAG ATCATTACCA TTAGAATATT TGACCGTGAG GAATATGAGA 
     2881 AAGAGTGCAG TTTCTCCCTT GTGCTTGAGG AACCAAAATG GATAAGAAGA GGAATGAAAG 
     2941 GTGGCTTCAC AATAACAGGA AAATACCTGT TTGGCCAACC TGTCTTCAGG AAGGTTCATG 
     3001 CTAGAGAACA TCCGATTCTC TCTACTGTAA TCACCATTGC AGACGAATAT GATGACAAGC 
     3061 AGCCACTGAC CAGCAAAGAG GAAGAGGAGA GGCGCATTGC AGAAATGGGG CGCCCCATCC 
     3121 TGGGAGAGCA CACCAAGTTG GAAGTGATCA TTGAAGAATC CTATGAATTC AAGAGTACTG 
     3181 TGGACAAACT CATTAAGAAG ACAAACCTGG CCCTTGTGGT TGGGACTAAC AGCTGGAGAG 
     3241 AACAGTTCAT TGAAGCTATC ACTGTCAGTG CTGGGGAAGA TGATGACGAC GATGAATGTG 
     3301 GGGAAGAGAA GCTGCCCTCC TGTTTCGATT ACGTGATGCA CTTTCTGACT GTGTTCTGGA 
     3361 AGGTCCTGTT TGCCTTCGTC CCCCCTACTG AATACTGGAA TGGCTGGGCG TGTTTCATTG 
     3421 TCTCCATCCT CATGATTGGC CTACTGACAG CTTTCATTGG AGACCTGGCT TCCCACTTTG 
     3481 GCTGCACCAT TGGCCTGAAA GATTCTGTGA CTGCAGTCGT GTTCGTCGCA CTTGGAACAT 
     3541 CAGTGCCAGA CACATTTGCC AGCAAAGTGG CAGCCACCCA GGACCAGTAT GCAGACGCCT 
     3601 CCATAGGTAA CGTCACGGGC AGCAACGCGG TGAATGTCTT CCTGGGAATC GGTGTGGCCT 
     3661 GGTCCATCGC TGCCATCTAC CACGCAGCCA ATGGGGAACA GTTCAAAGTG TCCCCTGGCA 
     3721 CACTAGCTTT CTCTGTCACT CTCTTCACCA TTTTTGCTTT CATCAATGTG GGGGTGCTGC 
     3781 TGTATCGGCG GAGGCCAGAA ATCGGAGGTG AGCTGGGTGG GCCCCGGACT GCCAAGCTCC 
     3841 TCACATCCTG CCTCTTTGTG CTCCTATGGC TCTTGTACAT TTTCTTCTCC TCCCTGGAGG 
     3901 CCTACTGCCA CATAAAAGGC TTCACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3961 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     4021 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     4081 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4141 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4201 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4261 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4321 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4381 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4441 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4501 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4561 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4621 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4681 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4741 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4801 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4861 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4921 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4981 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5041 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5101 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5161 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5221 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5281 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5341 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5401 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5461 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5521 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5581 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5641 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5701 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5761 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5821 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5881 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5941 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     6001 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     6061 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6121 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6181 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6241 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6301 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6361 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6421 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6481 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6541 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6841 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6901 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6961 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     7021 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     7081 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7141 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7201 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7261 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7321 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7381 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7441 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7501 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7561 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7621 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7681 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7741 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7801 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7861 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7921 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7981 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     8041 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     8101 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8161 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8221 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8281 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8341 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8401 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8461 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8521 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8581 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8641 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8701 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8761 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8821 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8881 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8941 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     9001 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     9061 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9121 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9181 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9241 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9301 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9361 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9421 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//