LOCUS       RG235072                9401 bp    DNA     circular  SYN 
DEFINITION  THBS3 (tGFP-tagged) - Homo sapiens thrombospondin 3 (THBS3), transcript variant 2
ACCESSION   NM_001252607
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3882..4586 
                     /label="GFP"  
     misc_feature    4633..5218 
                     /label="PolyA signal"  
     misc_feature    7310..7955 
                     /label="ColE1"  
     misc_feature    5672..6466 
                     /label="Neo"  
     misc_feature    5303..5605 
                     /label="SV40 ori"  
     misc_feature    7970..8830 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3867 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="THBS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACGCAG GAACTTCGGG GGGCCCTGGC TCTTCTCCTC CTTTGCTTTT 
     1081 TCACATCTGC CAGTCAGGAT CTGCAGGTAA TTGACCTGCT GACTGTGGGC GAGTCTCGGC 
     1141 AGATGGTAGC TGTGGCAGAG AAGATCCGGA CAGCCTTGCT CACTGCTGGG GACATCTACC 
     1201 TCTTATCCAC CTTCCGCCTG CCCCCCAAGC AGGGTGGTGT CCTCTTTGGC CTCTATTCTC 
     1261 GCCAAGACAA CACTCGATGG CTGGAGGCCT CTGTTGTAGG CAAGATCAAC AAAGTACTGG 
     1321 TGCGATACCA GCGGGAGGAT GGCAAAGTCC ACGCCGTGAA CCTACAGCAA GCGGGCCTGG 
     1381 CTGATGGGCG CACACACACA GTTCTCCTGC GACTCCGAGG TCCCTCCAGA CCCAGCCCTG 
     1441 CCCTACATCT CTACGTGGAC TGCAAACTGG GTGACCAACA TGCAGGCCTT CCAGCACTGG 
     1501 CCCCCATTCC TCCAGCGGAG GTCGATGGGC TGGAGATTAG GACTGGACAG AAGGCGTATT 
     1561 TGAGGATGCA GGGCTTTGTG GAATCTATGA AAATTATTCT GGGTGGGTCC ATGGCCCGGG 
     1621 TAGGAGCCCT GAGTGAGTGT CCATTCCAAG GGGACGAGTC CATCCACAGT GCAGGGGAGC 
     1681 AGACCAAGGC GCTGGTCACC CAACTCACCC TCTTCAACCA GATCCTGGTG GAGCTGCGGG 
     1741 ATGATATACG AGACCAGGTG AAGGAAATGT CCCTGATCCG AAACACCATT ATGGAGTGTC 
     1801 AGGTGTGCGG CTTCCATGAG CAGCGTTCCC ACTGCAGCCC CAATCCCTGC TTCCGAGGTG 
     1861 TGGACTGCAT GGAAGTGTAC GAGTACCCAG GCTACCGCTG TGGGCCCTGC CCCCCTGGCC 
     1921 TGCAGGGCAA CGGCACCCAC TGCAGTGACA TCAATGAGTG TGCTCACGCT GACCCCTGTT 
     1981 TCCCGGGCTC CAGCTGCATC AACACCATGC CCGGCTTCCA CTGTGAGGCC TGTCCTCGAG 
     2041 GGTACAAGGG CACACAGGTG TCTGGTGTGG GCATTGACTA TGCCCGGGCC AGCAAACAGG 
     2101 TCTGCAATGA CATCGATGAA TGCAACGATG GCAACAATGG TGGCTGTGAC CCAAACTCCA 
     2161 TCTGCACCAA CACTGTGGGC TCTTTCAAGT GTGGTCCCTG CCGCCTGGGT TTCCTGGGCA 
     2221 ACCAGAGCCA GGGCTGCCTC CCAGCCCGGA CCTGCCACAG CCCAGCCCAC AGCCCCTGCC 
     2281 ACATCCATGC TCACTGTCTC TTTGAACGCA ATGGTGCAGT GTCCTGCCAG TGTAACGTGG 
     2341 GCTGGGCTGG GAATGGGAAC GTGTGTGGGA CTGACACAGA CATCGATGGC TACCCAGACC 
     2401 AAGCACTGCC CTGCATGGAC AACAACAAAC ACTGCAAACA GGACAACTGC CTTTTGACAC 
     2461 CCAACTCTGG GCAGGAAGAT GCTGATAATG ATGGTGTGGG GGACCAGTGT GATGATGATG 
     2521 CTGATGGGGA TGGGATCAAG AATGTTGAGG ACAACTGCCG GCTGTTCCCC AACAAAGACC 
     2581 AGCAGAACTC AGATACAGAT TCATTTGGTG ATGCCTGTGA CAATTGCCCC AACGTTCCCA 
     2641 ACAATGACCA GAAGGACACA GATGGCAATG GGGAAGGAGA TGCCTGTGAC AACGACGTGG 
     2701 ATGGGGATGG CATCCCCAAT GGATTGGACA ATTGCCCTAA AGTCCCCAAC CCACTACAGA 
     2761 CAGACAGGGA TGAGGACGGG GTGGGAGATG CTTGCGACAG CTGCCCTGAA ATGAGCAATC 
     2821 CTACCCAGAC AGATGCAGAC AGCGACCTGG TGGGGGATGT CTGTGATACT AATGAAGACA 
     2881 GCGATGGGGA TGGGCATCAG GACACCAAGG ACAACTGCCC ACAGCTGCCA AATAGCTCCC 
     2941 AGCTGGACTC TGATAACGAT GGACTTGGAG ATGAGTGTGA TGGGGATGAT GACAATGATG 
     3001 GCATCCCAGA TTATGTGCCT CCTGGTCCCG ATAACTGCCG CCTGGTACCC AATCCCAATC 
     3061 AGAAGGACTC AGATGGCAAT GGCGTTGGTG ATGTGTGTGA GGATGACTTT GACAATGATG 
     3121 CTGTGGTCGA CCCCCTGGAT GTGTGTCCTG AAAGTGCAGA GGTAACGCTT ACGGATTTTC 
     3181 GGGCCTATCA GACCGTCGTC CTGGATCCTG AGGGTGATGC TCAGATTGAC CCAAACTGGG 
     3241 TTGTGCTCAA CCAGGGCATG GAAATCGTTC AGACCATGAA CAGTGACCCT GGCTTGGCAG 
     3301 TTGGATACAC GGCCTTCAAT GGTGTGGACT TTGAAGGCAC CTTCCATGTG AACACAGTGA 
     3361 CTGATGATGA CTACGCAGGC TTTCTCTTCA GTTATCAAGA CAGTGGCCGC TTCTACGTAG 
     3421 TCATGTGGAA GCAGACCGAG CAGACCTACT GGCAGGCTAC ACCCTTCCGG GCGGTTGCCC 
     3481 AGCCCGGGCT GCAGCTCAAG GCAGTGACAT CAGTGTCTGG CCCAGGTGAG CACCTCCGAA 
     3541 ATGCCCTGTG GCATACTGGC CACACCCCTG ATCAGGTACG ACTGCTGTGG ACAGACCCAC 
     3601 GAAATGTGGG CTGGCGGGAC AAGACCTCCT ATCGCTGGCA GCTTCTGCAC CGGCCTCAAG 
     3661 TTGGCTACAT TCGGGTGAAG CTCTATGAGG GACCCCAGCT TGTGGCGGAT TCTGGGGTGA 
     3721 TCATTGACAC ATCCATGCGA GGGGGGCGTC TTGGTGTATT CTGCTTCTCC CAAGAAAACA 
     3781 TAATTTGGTC CAATCTCCAG TATCGATGCA ATGACACAGT GCCTGAGGAC TTTGAGCCAT 
     3841 TCCGGAGGCA GCTGCTCCAG GGAAGGGTGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3901 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3961 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     4021 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     4081 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     4141 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4201 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4261 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4321 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4381 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4441 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4501 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4561 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4621 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4681 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4741 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4801 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4861 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4921 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4981 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5041 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5101 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5161 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5221 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5281 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5341 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5401 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5461 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5521 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5581 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5641 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5701 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5761 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5821 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5881 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5941 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     6001 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     6061 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     6121 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6181 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6241 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6301 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6361 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6421 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6481 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6541 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6601 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6661 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6721 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6781 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6841 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6901 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6961 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     7021 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     7081 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     7141 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7201 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7261 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7321 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7381 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7441 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7501 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7561 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7621 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7681 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7741 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7801 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7861 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7921 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7981 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     8041 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     8101 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     8161 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8221 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8281 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8341 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8401 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8461 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8521 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8581 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8641 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8701 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8761 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8821 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8881 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8941 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     9001 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     9061 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     9121 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9181 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9241 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9301 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9361 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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